Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9RQI5 (GLGB_BERDE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
OrganismBergeriella denitrificans (Neisseria denitrificans)
Taxonomic identifier494 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeBergeriella

Protein attributes

Sequence length762 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7627621,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188719

Sites

Active site4171Nucleophile By similarity
Active site4701Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9RQI5 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 9922F80406DDE3E3

FASTA76286,367
        10         20         30         40         50         60 
MNRNRHIRRG YHPEAGERQI IDSLFAATHS DPFAYLGRHR VNDEREAVRV LRPDAHHIDI 

        70         80         90        100        110        120 
IDRHTGAVIM PSEKIDERGL FAAVLPEHAP DYALLVTYHE GEAAVREEDD YRFGSALQHT 

       130        140        150        160        170        180 
DAWLLGEGTH LRPYETLGAH FAEMDGVSGV RFAVWAPNAR RVSVIGEFNG WDSRRHAMRP 

       190        200        210        220        230        240 
HTGNGLWDIF IPGVGLNALY KFSVLDANGN IREKADPYAF GAELRPTTAS VVRGLPAKAE 

       250        260        270        280        290        300 
APAFRRRANS VEAPISIYEV HLGSWRRNPE NNYWLTYTQL ADELVNYVKD MGFTHIELLP 

       310        320        330        340        350        360 
LSEYPFDGSW GYQATGLYAP TSRFGSPDEL KALIDAAHAA GISVILDWVA GHFPTDDHGL 

       370        380        390        400        410        420 
NTFDGTALYE HADPREGYHQ DWNTLIYNFG RNEVKNFLQG NALYWIERFG FDGIRVDAVA 

       430        440        450        460        470        480 
SMIYRNYSRK DGEWIPNRYG GSENLEAIAF LRQTNAVLKS ETPGAGSFAE ESTSFADVTR 

       490        500        510        520        530        540 
EAGLNFDFKW NMGWMNDTLR YMQEDPVHRK YHHGKMTFGM MYQYSENFVL PLSHDEVVHG 

       550        560        570        580        590        600 
KRSLLGKMPG DCWQQFANLR AYYGFMYGFP GKKLLFMGNE FAQGREWNYQ EGLDWHLLDE 

       610        620        630        640        650        660 
AGGWHKGVQD YVRDLNHIYT AHAPLYQLDQ QPEGFEWLVA DDSDNSVFVF ERRDRAGNRI 

       670        680        690        700        710        720 
IVISNFTPVV REHYRFGVNA PGRYTEILNS DRTQYQGSGI ANGADITAEN VPSHGKAQSL 

       730        740        750        760 
SLTLPPLATV YLYQKAAPAT EIQTALRADK QPAVKDKQAK AK 

« Hide

References

[1]"Molecular cloning, functional expression and purification of a glucan branching enzyme from Neisseria denitrificans."
Buettcher V., Quanz M., Willmitzer L.
Biochim. Biophys. Acta 1432:406-412(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 14686 / DSM 17675 / CIP 72.16 / JCM 21446 / NBRC 102155 / NCTC 10295.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF102867 Genomic DNA. Translation: AAF04747.1.

3D structure databases

ProteinModelPortalQ9RQI5.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA2.4.1.18. 3588.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_BERDE
AccessionPrimary (citable) accession number: Q9RQI5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries