Reviewed, UniProtKB/Swiss-Prot Q9RQ55 (ILVC_BUCDN)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:

ilvC

Organism

Buchnera aphidicola subsp. Diuraphis noxia

Taxonomic identifier

118101 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 489	489	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000151289
Sites
	Active site	131	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9RQ55-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: FFA5FD7B078DEF2C	FASTA	489	55,886
	10 20 30 40 50 60 MNYFNTLNFR QKINQIKKCR FMEKKEFNKK NEILKNKKIV IVGCGSQGLN QGLNMRDSGL 70 80 90 100 110 120 NISYALRKNS ILKKNQSWIN ATKNNFLVGD YESLIPNADL VINLTPDKQH SNVVKELQKL 130 140 150 160 170 180 MKKDACLGYS HGFNIVEKLR NIRKDITVIM VAPKCPGTEV REEYKRGFGV GTLIAVHNEN 190 200 210 220 230 240 DYSNMGLEVA KAWAFSTGGH RAGVLESSFV AEVKSDLMGE QTILCGMLQT ASLVCYEKLI 250 260 270 280 290 300 TTQNHPGYAG KLIQFGWETI TESLKHGGIT LMMDRLSNSS KIRAFKLSQE IKEFSQNLFQ 310 320 330 340 350 360 KHMDDIISGD FSSKMIQDWK NKDQNLLNWR YKTKNTSFEQ SPDYDKKILE QEYYDHGILM 370 380 390 400 410 420 VAILKAGIEL SFETMIQSGI MEESAYYESL HELPLIANTI ARKKLYEMNM VISDTAEYGN 430 440 450 460 470 480 YLFSESAYPI LKEFVKNINN TDLGSALPRN SVNNIELYNV NTMIRNHPIE IIGRKLRSYM KNMKTIIVG

References

[1]	"Sequence evolution in bacterial endosymbionts having extreme base compositions." Clark M.A., Moran N.A., Baumann P. Mol. Biol. Evol. 16:1586-1598(1999) [PubMed: 10555290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF130812 Genomic DNA. Translation: AAF13799.1.
3D structure databases
HSSP	HSSP built from PDB template 1NP3 based on UniProtKB Q9HVA2.
ModBase	Search...
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR014359. KetolA_reductoisomerase_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
PIRSF	PIRSF000116. IlvC_gammaproteo. 1 hit.
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_BUCDN

Accession

Primary (citable) accession number: Q9RQ55

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)