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Q9RPY1 (VIRB4_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type IV secretion system protein virB4
Gene names
Name:virB4
Ordered Locus Names:BRA0066, BS1330_II0066
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length831 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The VirB system could be required for the establishment of the replication niche in the host.

Induction

Specifically induced within macrophages by phagosome acidification. Induced at 37 degrees Celsius in minimal medium, suggesting that nutritional stress is a regulating signal.

Miscellaneous

Transcription of the operon is maximal in early exponential phase.

Sequence similarities

Belongs to the TrbE/VirB4 family.

Sequence caution

The sequence AAD56614.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processVirulence
   LigandATP-binding
Nucleotide-binding
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 831831Type IV secretion system protein virB4
PRO_0000290171

Regions

Nucleotide binding457 – 4648ATP Potential

Experimental info

Sequence conflict801W → R in AAD56614. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9RPY1 [UniParc].

Last modified June 12, 2007. Version 2.
Checksum: 3C8D3695FA986EE4

FASTA83194,546
        10         20         30         40         50         60 
MGAQSKYAQQ LNNERSLAPF IPFRSQVGPT TVITRDGDFV RTWRIAGLAF ETQDKEELLI 

        70         80         90        100        110        120 
RKDQLNTLFR AIASNNVALW SHNVRRRTWD HLKSFFSNPF CDALDKKYYG SFSGYRMMSN 

       130        140        150        160        170        180 
ELYLTVIYRP VPAKISRLFN VAVHRSHAEI LQEQQLAIRK LDEIGNQIET SLRRYGGDDG 

       190        200        210        220        230        240 
RGIEVLSTYE DKHGALCSQQ LEFYNFLLSG EWQKVRVPSC PLDEYLGTGW VYAGTETIEI 

       250        260        270        280        290        300 
RTANATRYAR GIDFKDYASH TEPGILNGLM YSDYEYVITQ SFSFMTKRDG KEFLTRQKQR 

       310        320        330        340        350        360 
LQNTEDGSAS QIMEMDIAID QLGRGDFVMG EYHYSLLVFA EDMETVRHNT SHAMNILQDN 

       370        380        390        400        410        420 
GFLATVIATA TDAAFYAQLP CNWRYRPRVA GLTSLNFAGL SCFHNFRAGK RDGNPWGQAL 

       430        440        450        460        470        480 
TLLKTPSGQP AYLNFHYSKG DEDNFDKKLL GNTRIIGQSG AGKTVLMNFC LAQAQKYLHN 

       490        500        510        520        530        540 
APMGMCNVFF DKDQGAKGTI LAIGGKYLAI RNGEPTGFNP FQMEPTAGNI LFLEKLVQVL 

       550        560        570        580        590        600 
VSRDGQHVTT TDESRISHAI RTVMRMRPEL RRLSTVLQNV TEGSDRQDRE NSVAKRLAKW 

       610        620        630        640        650        660 
CFDDGTGKRG TFWWVLDCPQ DQIDFNTHSN YGFDGTDFLD NADVRTPISM YLLHRMELAI 

       670        680        690        700        710        720 
DGRRFIYWMD EAWKWVDDEA FSEFANNKQL TIRKQNGLGV FATQMPSSLL NSKVASALVQ 

       730        740        750        760        770        780 
QVATEIYLPN PKADYHEYTD GFKVTNEEFD IIRSMSEESR MFLVKQGHHS MICRLELNGF 

       790        800        810        820        830 
DDELAILSGS SDNNELLDQV IAEVGDDPSV WLPVFQERRK ARIASSKSTG R 

« Hide

References

« Hide 'large scale' references
[1]"A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis Ptl type IV secretion systems is essential for intracellular survival of Brucella suis."
O'Callaghan D., Cazevieille C., Allardet-Servent A., Boschiroli M.L., Bourg G., Foulongne V., Frutos P., Kulakov Y., Ramuz M.
Mol. Microbiol. 33:1210-1220(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 1330.
[2]"The Brucella suis virB operon is induced intracellularly in macrophages."
Boschiroli M.L., Ouahrani-Bettache S., Foulongne V., Michaux-Charachon S., Bourg G., Allardet-Servent A., Cazevieille C., Liautard J.P., Ramuz M., O'Callaghan D.
Proc. Natl. Acad. Sci. U.S.A. 99:1544-1549(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], EXPRESSION CONDITIONS.
Strain: 1330.
[3]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[4]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF141604 Genomic DNA. Translation: AAD56614.1. Different initiation.
AE014292 Genomic DNA. Translation: AAN33278.1.
CP002998 Genomic DNA. Translation: AEM19558.1.
RefSeqNP_699273.1. NC_004311.2.
YP_005613784.1. NC_017250.1.

3D structure databases

ProteinModelPortalQ9RPY1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204722.BRA0066.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN33278; AAN33278; BRA0066.
AEM19558; AEM19558; BS1330_II0066.
GeneID1164503.
12139500.
KEGGbms:BRA0066.
bsi:BS1330_II0066.
PATRIC17792933. VBIBruSui107850_2285.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3451.
HOGENOMHOG000119263.
KOK03199.
OMAFKDYASH.
OrthoDBEOG6HMX9P.
ProtClustDBCLSK884268.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR004346. CagE_TrbE_VirB.
IPR018145. CagE_TrbE_VirB_cntrl_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF03135. CagE_TrbE_VirB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00929. VirB4_CagE. 1 hit.
ProtoNetSearch...

Other

PROQ9RPY1.

Entry information

Entry nameVIRB4_BRUSU
AccessionPrimary (citable) accession number: Q9RPY1
Secondary accession number(s): G0KER4, Q8FXK3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: June 12, 2007
Last modified: April 16, 2014
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella suis

Brucella suis (strain 1330): entries and gene names