Q9RPY1 (VIRB4_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type IV secretion system protein virB4

Gene names

Name:	virB4
Ordered Locus Names:	BRA0066, BS1330_II0066

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	831 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The VirB system could be required for the establishment of the replication niche in the host.
Induction	Specifically induced within macrophages by phagosome acidification. Induced at 37 degrees Celsius in minimal medium, suggesting that nutritional stress is a regulating signal.
Miscellaneous	Transcription of the operon is maximal in early exponential phase.
Sequence similarities	Belongs to the TrbE/VirB4 family.
Sequence caution	The sequence AAD56614.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Virulence
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 831	831	Type IV secretion system protein virB4		PRO_0000290171
Regions
	Nucleotide binding	457 – 464	8	ATP Potential
Experimental info
	Sequence conflict	80	1	W → R in AAD56614. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9RPY1 [UniParc]. Last modified June 12, 2007. Version 2. Checksum: 3C8D3695FA986EE4	FASTA	831	94,546
	10 20 30 40 50 60 MGAQSKYAQQ LNNERSLAPF IPFRSQVGPT TVITRDGDFV RTWRIAGLAF ETQDKEELLI 70 80 90 100 110 120 RKDQLNTLFR AIASNNVALW SHNVRRRTWD HLKSFFSNPF CDALDKKYYG SFSGYRMMSN 130 140 150 160 170 180 ELYLTVIYRP VPAKISRLFN VAVHRSHAEI LQEQQLAIRK LDEIGNQIET SLRRYGGDDG 190 200 210 220 230 240 RGIEVLSTYE DKHGALCSQQ LEFYNFLLSG EWQKVRVPSC PLDEYLGTGW VYAGTETIEI 250 260 270 280 290 300 RTANATRYAR GIDFKDYASH TEPGILNGLM YSDYEYVITQ SFSFMTKRDG KEFLTRQKQR 310 320 330 340 350 360 LQNTEDGSAS QIMEMDIAID QLGRGDFVMG EYHYSLLVFA EDMETVRHNT SHAMNILQDN 370 380 390 400 410 420 GFLATVIATA TDAAFYAQLP CNWRYRPRVA GLTSLNFAGL SCFHNFRAGK RDGNPWGQAL 430 440 450 460 470 480 TLLKTPSGQP AYLNFHYSKG DEDNFDKKLL GNTRIIGQSG AGKTVLMNFC LAQAQKYLHN 490 500 510 520 530 540 APMGMCNVFF DKDQGAKGTI LAIGGKYLAI RNGEPTGFNP FQMEPTAGNI LFLEKLVQVL 550 560 570 580 590 600 VSRDGQHVTT TDESRISHAI RTVMRMRPEL RRLSTVLQNV TEGSDRQDRE NSVAKRLAKW 610 620 630 640 650 660 CFDDGTGKRG TFWWVLDCPQ DQIDFNTHSN YGFDGTDFLD NADVRTPISM YLLHRMELAI 670 680 690 700 710 720 DGRRFIYWMD EAWKWVDDEA FSEFANNKQL TIRKQNGLGV FATQMPSSLL NSKVASALVQ 730 740 750 760 770 780 QVATEIYLPN PKADYHEYTD GFKVTNEEFD IIRSMSEESR MFLVKQGHHS MICRLELNGF 790 800 810 820 830 DDELAILSGS SDNNELLDQV IAEVGDDPSV WLPVFQERRK ARIASSKSTG R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis Ptl type IV secretion systems is essential for intracellular survival of Brucella suis." O'Callaghan D., Cazevieille C., Allardet-Servent A., Boschiroli M.L., Bourg G., Foulongne V., Frutos P., Kulakov Y., Ramuz M. Mol. Microbiol. 33:1210-1220(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1330.
[2]	"The Brucella suis virB operon is induced intracellularly in macrophages." Boschiroli M.L., Ouahrani-Bettache S., Foulongne V., Michaux-Charachon S., Bourg G., Allardet-Servent A., Cazevieille C., Liautard J.P., Ramuz M., O'Callaghan D. Proc. Natl. Acad. Sci. U.S.A. 99:1544-1549(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], EXPRESSION CONDITIONS. Strain: 1330.
[3]	"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[4]	"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF141604 Genomic DNA. Translation: AAD56614.1. Different initiation. AE014292 Genomic DNA. Translation: AAN33278.1. CP002998 Genomic DNA. Translation: AEM19558.1.
RefSeq	NP_699273.1. NC_004311.2. YP_005613784.1. NC_017250.1.
3D structure databases
ProteinModelPortal	Q9RPY1.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BRA0066.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN33278; AAN33278; BRA0066. AEM19558; AEM19558; BS1330_II0066.
GeneID	1164503. 12139500.
KEGG	bms:BRA0066. bsi:BS1330_II0066.
PATRIC	17792933. VBIBruSui107850_2285.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3451.
HOGENOM	HOG000119263.
KO	K03199.
OMA	FKDYASH.
ProtClustDB	CLSK884268.
Family and domain databases
InterPro	IPR004346. CagE_TrbE_VirB. IPR018145. CagE_TrbE_VirB_cntrl_dom. [Graphical view]
Pfam	PF03135. CagE_TrbE_VirB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00929. VirB4_CagE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

VIRB4_BRUSU

Accession

Primary (citable) accession number: Q9RPY1
Secondary accession number(s): G0KER4, Q8FXK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	June 12, 2007
Last modified:	May 1, 2013
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program