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Q9RPU5 (HPAG_SALDU) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase

Including the following 2 domains:

  1. 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase
    Short name=HHDD isomerase
    EC=5.3.3.10
    Alternative name(s):
    5-carboxymethyl-2-hydroxymuconate Delta-isomerase
  2. 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase
    EC=4.1.1.68
    Alternative name(s):
    5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
    Short name=OPET decarboxylase
Gene names
Name:hpaG
OrganismSalmonella dublin
Taxonomic identifier98360 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length429 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5-tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7-dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate).

Catalytic activity

5-carboxymethyl-2-hydroxymuconate = 5-carboxy-2-oxohept-3-enedioate.

5-oxopent-3-ene-1,2,5-tricarboxylate = 2-oxohept-3-enedioate + CO2.

Cofactor

Magnesium By similarity.

Pathway

Aromatic compound metabolism; 4-hydroxyphenylacetate degradation; pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate: step 4/7.

Aromatic compound metabolism; 4-hydroxyphenylacetate degradation; pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate: step 5/7.

Subunit structure

Monomer By similarity.

Sequence similarities

Belongs to the FAH family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4294294-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase
PRO_0000156834

Regions

Repeat1 – 215215Approximate
Repeat216 – 429214Approximate

Sites

Metal binding2761Divalent metal cation By similarity
Metal binding2781Divalent metal cation By similarity
Metal binding3071Divalent metal cation By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9RPU5 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: D32723999AC4084E

FASTA42947,186
        10         20         30         40         50         60 
MKGTVFAVAL NHRSQLDAWQ EAFSQPPYNA PPKTAVWFIK PRNTVIRHGE PILYPQGEKV 

        70         80         90        100        110        120 
LSGATVALIV GKTASRKRSE AAAEYIAGYA LANEVSLPEE SFYRPAIKAK CRDGFCPLGE 

       130        140        150        160        170        180 
MAPLSDVDNL TIITEINGRE ADHWNTADLQ RSAAQLLSAL SEFATLNPGD AILLGTPQNR 

       190        200        210        220        230        240 
VALRPGDRVR ILAKGLPALE NPVVAEDEFA RNQTFTWPLS ATGTLFALGL NYADHASELA 

       250        260        270        280        290        300 
FTPPKEPLVF IKAPNTFTEH HQTSVRPNNV EYMHYEAELV VVIGKTARKV SEAEAMEYVA 

       310        320        330        340        350        360 
GYTVCNDYAI RDYLENYYRP NLRVKSRDGL TPIGPWIVDK EAVSDPHNLT LRTFVNGELR 

       370        380        390        400        410        420 
QEGTTADLIF SIPFLISYLS EFMTLQPGDM IATGTPKGLS DVVPGDEVVL EIKGVGRLVN 


QIVCEESAN 

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References

[1]"Characterization of the hpa genetic locus from Salmonella dublin."
Galyov E.E., Wood M.W., Hedges S.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 2229.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF144422 Genomic DNA. Translation: AAD53501.1.

3D structure databases

ProteinModelPortalQ9RPU5.
SMRQ9RPU5. Positions 1-428.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00208; UER00419.
UPA00208; UER00420.

Family and domain databases

Gene3D3.90.850.10. 2 hits.
InterProIPR002529. Fumarylacetoacetase_C.
IPR011234. Fumarylacetoacetase_C-rel.
IPR012684. HPA_isomer/decarb_C.
IPR012686. HPA_isomer/decarb_N.
[Graphical view]
PfamPF01557. FAA_hydrolase. 2 hits.
[Graphical view]
SUPFAMSSF56529. SSF56529. 2 hits.
TIGRFAMsTIGR02303. HpaG-C-term. 1 hit.
TIGR02305. HpaG-N-term. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHPAG_SALDU
AccessionPrimary (citable) accession number: Q9RPU5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: October 16, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways