Q9RPU5 (HPAG_SALDU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase

Including the following 2 domains:

1. 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase
   Short name=HHDD isomerase
   EC=5.3.3.10
   Alternative name(s):
   5-carboxymethyl-2-hydroxymuconate Delta-isomerase
2. 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase
   EC=4.1.1.68
   Alternative name(s):
   5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
   Short name=OPET decarboxylase

Gene names

Name:

hpaG

Organism

Salmonella dublin

Taxonomic identifier

98360 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5-tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7-dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate).
Catalytic activity	5-carboxymethyl-2-hydroxymuconate = 5-carboxy-2-oxohept-3-enedioate. 5-oxopent-3-ene-1,2,5-tricarboxylate = 2-oxohept-3-enedioate + CO2.
Cofactor	Magnesium By similarity.
Pathway	Aromatic compound metabolism; 4-hydroxyphenylacetate degradation; pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate: step 4/7. Aromatic compound metabolism; 4-hydroxyphenylacetate degradation; pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate: step 5/7.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the FAH family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Domain	Repeat
Ligand	Metal-binding
Molecular function	Isomerase Lyase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	4-hydroxyphenylacetate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	5-carboxymethyl-2-hydroxymuconate delta-isomerase activity Inferred from electronic annotation. Source: EC 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase		PRO_0000156834
Regions
	Repeat	1 – 215	215	Approximate
	Repeat	216 – 429	214	Approximate
Sites
	Metal binding	276	1	Divalent metal cation By similarity
	Metal binding	278	1	Divalent metal cation By similarity
	Metal binding	307	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9RPU5 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: D32723999AC4084E	FASTA	429	47,186
	10 20 30 40 50 60 MKGTVFAVAL NHRSQLDAWQ EAFSQPPYNA PPKTAVWFIK PRNTVIRHGE PILYPQGEKV 70 80 90 100 110 120 LSGATVALIV GKTASRKRSE AAAEYIAGYA LANEVSLPEE SFYRPAIKAK CRDGFCPLGE 130 140 150 160 170 180 MAPLSDVDNL TIITEINGRE ADHWNTADLQ RSAAQLLSAL SEFATLNPGD AILLGTPQNR 190 200 210 220 230 240 VALRPGDRVR ILAKGLPALE NPVVAEDEFA RNQTFTWPLS ATGTLFALGL NYADHASELA 250 260 270 280 290 300 FTPPKEPLVF IKAPNTFTEH HQTSVRPNNV EYMHYEAELV VVIGKTARKV SEAEAMEYVA 310 320 330 340 350 360 GYTVCNDYAI RDYLENYYRP NLRVKSRDGL TPIGPWIVDK EAVSDPHNLT LRTFVNGELR 370 380 390 400 410 420 QEGTTADLIF SIPFLISYLS EFMTLQPGDM IATGTPKGLS DVVPGDEVVL EIKGVGRLVN QIVCEESAN

References

[1]	"Characterization of the hpa genetic locus from Salmonella dublin." Galyov E.E., Wood M.W., Hedges S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 2229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF144422 Genomic DNA. Translation: AAD53501.1.
3D structure databases
ProteinModelPortal	Q9RPU5.
SMR	Q9RPU5. Positions 1-428.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00208; UER00419. UPA00208; UER00420.
Family and domain databases
Gene3D	3.90.850.10. 2 hits.
InterPro	IPR002529. Fumarylacetoacetase_C. IPR011234. Fumarylacetoacetase_C-rel. IPR012684. HPA_isomer/decarb_C. IPR012686. HPA_isomer/decarb_N. [Graphical view]
Pfam	PF01557. FAA_hydrolase. 2 hits. [Graphical view]
SUPFAM	SSF56529. Fumarylacetoacetase_C-rel. 2 hits.
TIGRFAMs	TIGR02303. HpaG-C-term. 1 hit. TIGR02305. HpaG-N-term. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPAG_SALDU

Accession

Primary (citable) accession number: Q9RPU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	March 6, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program