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Protein

Rhamnolipids biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase

Gene

rhlG

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for the synthesis of the beta-hydroxy acid moiety of rhamnolipids.

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei148 – 1481SubstrateBy similarity
Active sitei162 – 1621Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 3825NADPBy similarityAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15945.
UniPathwayiUPA00657.

Names & Taxonomyi

Protein namesi
Recommended name:
Rhamnolipids biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Gene namesi
Name:rhlG
Ordered Locus Names:PA3387
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3387.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 256256Rhamnolipids biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductasePRO_0000054755Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3387.

Structurei

Secondary structure

1
256
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni3 – 53Combined sources
Beta strandi11 – 155Combined sources
Turni16 – 183Combined sources
Helixi20 – 3112Combined sources
Beta strandi35 – 395Combined sources
Helixi43 – 5311Combined sources
Beta strandi59 – 613Combined sources
Helixi69 – 8214Combined sources
Beta strandi87 – 915Combined sources
Helixi107 – 11610Combined sources
Helixi118 – 13417Combined sources
Beta strandi137 – 1393Combined sources
Beta strandi141 – 1466Combined sources
Helixi149 – 1513Combined sources
Helixi162 – 18019Combined sources
Helixi181 – 1833Combined sources
Beta strandi185 – 1928Combined sources
Turni198 – 2003Combined sources
Helixi201 – 2055Combined sources
Helixi207 – 2159Combined sources
Helixi225 – 23612Combined sources
Helixi238 – 2403Combined sources
Beta strandi247 – 2515Combined sources
Turni252 – 2554Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B4QX-ray2.30A/B1-256[»]
ProteinModelPortaliQ9RPT1.
SMRiQ9RPT1. Positions 1-256.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9RPT1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
InParanoidiQ9RPT1.
OMAiFSCIQQL.
OrthoDBiEOG6N3CR8.
PhylomeDBiQ9RPT1.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.

Sequencei

Sequence statusi: Complete.

Q9RPT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPYFSLAGR IALVTGGSRG IGQMIAQGLL EAGARVFICA RDAEACADTA
60 70 80 90 100
TRLSAYGDCQ AIPADLSSEA GARRLAQALG ELSARLDILV NNAGTSWGAA
110 120 130 140 150
LESYPVSGWE KVMQLNVTSV FSCIQQLLPL LRRSASAENP ARVINIGSVA
160 170 180 190 200
GISAMGEQAY AYGPSKAALH QLSRMLAKEL VGEHINVNVI APGRFPSRMT
210 220 230 240 250
RHIANDPQAL EADSASIPMG RWGRPEEMAA LAISLAGTAG AYMTGNVIPI

DGGFHL
Length:256
Mass (Da):26,831
Last modified:May 1, 2000 - v1
Checksum:i3DB7B481F34C89A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148964 Genomic DNA. Translation: AAD53514.1.
AE004091 Genomic DNA. Translation: AAG06775.1.
PIRiF83221.
RefSeqiNP_252077.1. NC_002516.2.
WP_003113116.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG06775; AAG06775; PA3387.
GeneIDi880033.
KEGGipae:PA3387.
PATRICi19841377. VBIPseAer58763_3546.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148964 Genomic DNA. Translation: AAD53514.1.
AE004091 Genomic DNA. Translation: AAG06775.1.
PIRiF83221.
RefSeqiNP_252077.1. NC_002516.2.
WP_003113116.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B4QX-ray2.30A/B1-256[»]
ProteinModelPortaliQ9RPT1.
SMRiQ9RPT1. Positions 1-256.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3387.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06775; AAG06775; PA3387.
GeneIDi880033.
KEGGipae:PA3387.
PATRICi19841377. VBIPseAer58763_3546.

Organism-specific databases

PseudoCAPiPA3387.

Phylogenomic databases

eggNOGiCOG1028.
InParanoidiQ9RPT1.
OMAiFSCIQQL.
OrthoDBiEOG6N3CR8.
PhylomeDBiQ9RPT1.

Enzyme and pathway databases

UniPathwayiUPA00657.
BioCyciMetaCyc:MONOMER-15945.

Miscellaneous databases

EvolutionaryTraceiQ9RPT1.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Pseudomonas aeruginosa rhlG gene encodes an NADPH-dependent beta-ketoacyl reductase which is specifically involved in rhamnolipid synthesis."
    Campos-Garcia J., Caro A.D., Najera R., Miller-Maier R.M., Al-Tahhan R.A., Soberon-Chavez G.
    J. Bacteriol. 180:4442-4451(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiRHLG_PSEAE
AccessioniPrimary (citable) accession number: Q9RPT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 1, 2000
Last modified: May 27, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.