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Protein

Rhamnolipids biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase

Gene

rhlG

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for the synthesis of the beta-hydroxy acid moiety of rhamnolipids.

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathwayi: rhamnolipid biosynthesis

This protein is involved in the pathway rhamnolipid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway rhamnolipid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei148SubstrateBy similarity1
Active sitei162Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 38NADPBy similarityAdd BLAST25

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15945.
UniPathwayiUPA00657.

Names & Taxonomyi

Protein namesi
Recommended name:
Rhamnolipids biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Gene namesi
Name:rhlG
Ordered Locus Names:PA3387
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3387.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000547551 – 256Rhamnolipids biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductaseAdd BLAST256

Proteomic databases

PaxDbiQ9RPT1.
PRIDEiQ9RPT1.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3387.

Structurei

Secondary structure

1256
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni3 – 5Combined sources3
Beta strandi11 – 15Combined sources5
Turni16 – 18Combined sources3
Helixi20 – 31Combined sources12
Beta strandi35 – 39Combined sources5
Helixi43 – 53Combined sources11
Beta strandi59 – 61Combined sources3
Helixi69 – 82Combined sources14
Beta strandi87 – 91Combined sources5
Helixi107 – 116Combined sources10
Helixi118 – 134Combined sources17
Beta strandi137 – 139Combined sources3
Beta strandi141 – 146Combined sources6
Helixi149 – 151Combined sources3
Helixi162 – 180Combined sources19
Helixi181 – 183Combined sources3
Beta strandi185 – 192Combined sources8
Turni198 – 200Combined sources3
Helixi201 – 205Combined sources5
Helixi207 – 215Combined sources9
Helixi225 – 236Combined sources12
Helixi238 – 240Combined sources3
Beta strandi247 – 251Combined sources5
Turni252 – 255Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2B4QX-ray2.30A/B1-256[»]
ProteinModelPortaliQ9RPT1.
SMRiQ9RPT1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9RPT1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CHR. Bacteria.
ENOG410XNW1. LUCA.
InParanoidiQ9RPT1.
OMAiFSCIQQL.
PhylomeDBiQ9RPT1.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9RPT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPYFSLAGR IALVTGGSRG IGQMIAQGLL EAGARVFICA RDAEACADTA
60 70 80 90 100
TRLSAYGDCQ AIPADLSSEA GARRLAQALG ELSARLDILV NNAGTSWGAA
110 120 130 140 150
LESYPVSGWE KVMQLNVTSV FSCIQQLLPL LRRSASAENP ARVINIGSVA
160 170 180 190 200
GISAMGEQAY AYGPSKAALH QLSRMLAKEL VGEHINVNVI APGRFPSRMT
210 220 230 240 250
RHIANDPQAL EADSASIPMG RWGRPEEMAA LAISLAGTAG AYMTGNVIPI

DGGFHL
Length:256
Mass (Da):26,831
Last modified:May 1, 2000 - v1
Checksum:i3DB7B481F34C89A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148964 Genomic DNA. Translation: AAD53514.1.
AE004091 Genomic DNA. Translation: AAG06775.1.
PIRiF83221.
RefSeqiNP_252077.1. NC_002516.2.
WP_003113116.1. NZ_ASJY01000542.1.

Genome annotation databases

EnsemblBacteriaiAAG06775; AAG06775; PA3387.
GeneIDi880033.
KEGGipae:PA3387.
PATRICi19841377. VBIPseAer58763_3546.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148964 Genomic DNA. Translation: AAD53514.1.
AE004091 Genomic DNA. Translation: AAG06775.1.
PIRiF83221.
RefSeqiNP_252077.1. NC_002516.2.
WP_003113116.1. NZ_ASJY01000542.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2B4QX-ray2.30A/B1-256[»]
ProteinModelPortaliQ9RPT1.
SMRiQ9RPT1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3387.

Proteomic databases

PaxDbiQ9RPT1.
PRIDEiQ9RPT1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06775; AAG06775; PA3387.
GeneIDi880033.
KEGGipae:PA3387.
PATRICi19841377. VBIPseAer58763_3546.

Organism-specific databases

PseudoCAPiPA3387.

Phylogenomic databases

eggNOGiENOG4105CHR. Bacteria.
ENOG410XNW1. LUCA.
InParanoidiQ9RPT1.
OMAiFSCIQQL.
PhylomeDBiQ9RPT1.

Enzyme and pathway databases

UniPathwayiUPA00657.
BioCyciMetaCyc:MONOMER-15945.

Miscellaneous databases

EvolutionaryTraceiQ9RPT1.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRHLG_PSEAE
AccessioniPrimary (citable) accession number: Q9RPT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.