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Q9RPT1

- RHLG_PSEAE

UniProt

Q9RPT1 - RHLG_PSEAE

Protein

Rhamnolipids biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase

Gene

rhlG

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Required for the synthesis of the beta-hydroxy acid moiety of rhamnolipids.

    Catalytic activityi

    (3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei148 – 1481SubstrateBy similarity
    Active sitei162 – 1621Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi14 – 3825NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15945.
    UniPathwayiUPA00657.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rhamnolipids biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (EC:1.1.1.100)
    Alternative name(s):
    3-ketoacyl-acyl carrier protein reductase
    Gene namesi
    Name:rhlG
    Ordered Locus Names:PA3387
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA3387.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 256256Rhamnolipids biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductasePRO_0000054755Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi208964.PA3387.

    Structurei

    Secondary structure

    1
    256
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni3 – 53
    Beta strandi11 – 155
    Turni16 – 183
    Helixi20 – 3112
    Beta strandi35 – 395
    Helixi43 – 5311
    Beta strandi59 – 613
    Helixi69 – 8214
    Beta strandi87 – 915
    Helixi107 – 11610
    Helixi118 – 13417
    Beta strandi137 – 1393
    Beta strandi141 – 1466
    Helixi149 – 1513
    Helixi162 – 18019
    Helixi181 – 1833
    Beta strandi185 – 1928
    Turni198 – 2003
    Helixi201 – 2055
    Helixi207 – 2159
    Helixi225 – 23612
    Helixi238 – 2403
    Beta strandi247 – 2515
    Turni252 – 2554

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2B4QX-ray2.30A/B1-256[»]
    ProteinModelPortaliQ9RPT1.
    SMRiQ9RPT1. Positions 1-256.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9RPT1.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1028.
    OMAiWGREEEM.
    OrthoDBiEOG6N3CR8.
    PhylomeDBiQ9RPT1.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.

    Sequencei

    Sequence statusi: Complete.

    Q9RPT1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHPYFSLAGR IALVTGGSRG IGQMIAQGLL EAGARVFICA RDAEACADTA    50
    TRLSAYGDCQ AIPADLSSEA GARRLAQALG ELSARLDILV NNAGTSWGAA 100
    LESYPVSGWE KVMQLNVTSV FSCIQQLLPL LRRSASAENP ARVINIGSVA 150
    GISAMGEQAY AYGPSKAALH QLSRMLAKEL VGEHINVNVI APGRFPSRMT 200
    RHIANDPQAL EADSASIPMG RWGRPEEMAA LAISLAGTAG AYMTGNVIPI 250
    DGGFHL 256
    Length:256
    Mass (Da):26,831
    Last modified:May 1, 2000 - v1
    Checksum:i3DB7B481F34C89A3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF148964 Genomic DNA. Translation: AAD53514.1.
    AE004091 Genomic DNA. Translation: AAG06775.1.
    PIRiF83221.
    RefSeqiNP_252077.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG06775; AAG06775; PA3387.
    GeneIDi880033.
    KEGGipae:PA3387.
    PATRICi19841377. VBIPseAer58763_3546.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF148964 Genomic DNA. Translation: AAD53514.1 .
    AE004091 Genomic DNA. Translation: AAG06775.1 .
    PIRi F83221.
    RefSeqi NP_252077.1. NC_002516.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2B4Q X-ray 2.30 A/B 1-256 [» ]
    ProteinModelPortali Q9RPT1.
    SMRi Q9RPT1. Positions 1-256.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA3387.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG06775 ; AAG06775 ; PA3387 .
    GeneIDi 880033.
    KEGGi pae:PA3387.
    PATRICi 19841377. VBIPseAer58763_3546.

    Organism-specific databases

    PseudoCAPi PA3387.

    Phylogenomic databases

    eggNOGi COG1028.
    OMAi WGREEEM.
    OrthoDBi EOG6N3CR8.
    PhylomeDBi Q9RPT1.

    Enzyme and pathway databases

    UniPathwayi UPA00657 .
    BioCyci MetaCyc:MONOMER-15945.

    Miscellaneous databases

    EvolutionaryTracei Q9RPT1.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    ProtoNeti Search...

    Publicationsi

    1. "The Pseudomonas aeruginosa rhlG gene encodes an NADPH-dependent beta-ketoacyl reductase which is specifically involved in rhamnolipid synthesis."
      Campos-Garcia J., Caro A.D., Najera R., Miller-Maier R.M., Al-Tahhan R.A., Soberon-Chavez G.
      J. Bacteriol. 180:4442-4451(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiRHLG_PSEAE
    AccessioniPrimary (citable) accession number: Q9RPT1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3