Q9RNZ9 (TFDD_DELAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chloromuconate cycloisomerase
EC=5.5.1.7

Gene names

Name:

tfdD

Organism

Delftia acidovorans (Pseudomonas acidovorans) (Comamonas acidovorans)

Taxonomic identifier

80866 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Delftia

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate.
Cofactor	Manganese By similarity.
Pathway	Aromatic compound metabolism; 3-chlorocatechol degradation.
Induction	By 2,4-dichlorophenoxypropionic acid (2,4-DCPP). Ref.4
Miscellaneous	Different ratios of isoforms are detectable, depending on concentration of dichlorprop.
Sequence similarities	Belongs to the mandelate racemase/muconate lactonizing enzyme family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Manganese Metal-binding
Molecular function	Isomerase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW cellular amino acid catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	chloromuconate cycloisomerase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: InterPro muconate cycloisomerase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Chloromuconate cycloisomerase		PRO_0000171258
Sites
	Active site	165	1	Proton acceptor By similarity
	Active site	323	1	Proton donor By similarity
	Metal binding	194	1	Manganese By similarity
	Metal binding	220	1	Manganese By similarity
	Metal binding	245	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9RNZ9 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: 195D3101CA18B31C	FASTA	370	39,465
	10 20 30 40 50 60 MKIEAISTTI VDVPTRRPLQ MSFTTVHKQS YVIVQVTAGG LVGIGEGGSV GGPTWGSESA 70 80 90 100 110 120 ETIKVIIDNY LAPLLIGKDA SNLSEARALM DRAVTGNLSA KAAIDIALHD LKARALNLSI 130 140 150 160 170 180 ADLIGGTMRK SIPIAWTLAS GDTARDIDSA LEMIEARRHN RFKVKLGART PAQDLEHIRS 190 200 210 220 230 240 IVKAVGDKAS VRVDVNQGWD EQTASIWIPR LEEAGVELVE QPVPRANFGA LRRLTEQNGV 250 260 270 280 290 300 AILADESLSS LSSAFELARD RAVDAFSLKL CNMGGIANTL KVAAIAEAAG ISSYGGTMLD 310 320 330 340 350 360 STVGTAAALH VYATLPSLPY GCELIGPWVL SDRLTQQDLE IKDFEVHLPV GSGLGVDLDH 370 DKVRHYTRAA

References

[1]	"A transposon encoding the complete 2,4-dichlorophenoxyacetic acid degradation pathway in the alkalitolerant strain Delftia acidovorans P4a." Hoffmann D., Kleinsteuber S., Mueller R.H., Babel W. Microbiology 149:2545-2556(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: P4a.
[2]	"Development and application of PCR primers for the detection of the tfd genes in Delftia acidovorans P4a involved in the degradation of 2,4-D." Hoffmann D., Kleinsteuber S., Mueller R.H., Babel W. Acta Biotechnol. 21:321-331(2001) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 67-268. Strain: P4a.
[3]	"Physiological and genetic characteristics of two bacterial strains utilizing phenoxypropionate and phenoxyacetate herbicides." Mueller R.H., Kleinsteuber S., Babel W. Microbiol. Res. 156:121-131(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-263. Strain: MC1.
[4]	"Regulation of catabolic enzymes during long-term exposure of Delftia acidovorans MC1 to chlorophenoxy herbicides." Benndorf D., Davidson I., Babel W. Microbiology 150:1005-1014(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-16, INDUCTION BY 2,4-DCPP. Strain: MC1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY078159 Genomic DNA. Translation: AAK57008.2. AF176244 Genomic DNA. Translation: AAD55083.1.
RefSeq	YP_006961882.1. NC_019283.1.
3D structure databases
ProteinModelPortal	Q9RNZ9.
SMR	Q9RNZ9. Positions 1-369.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	13918339.
Enzyme and pathway databases
UniPathway	UPA00083.
Family and domain databases
InterPro	IPR013370. Chloromuconate_cycloisomerase. IPR018110. Mandel_Rmase/mucon_lact_enz_CS. IPR013342. Mandelate_racemase_C. IPR013341. Mandelate_racemase_N. IPR001354. MR_MLE. [Graphical view]
PANTHER	PTHR13794. PTHR13794. 1 hit. PTHR13794:SF12. PTHR13794:SF12. 1 hit.
Pfam	PF02746. MR_MLE_N. 1 hit. [Graphical view]
SMART	SM00922. MR_MLE. 1 hit. [Graphical view]
TIGRFAMs	TIGR02534. mucon_cyclo. 1 hit.
PROSITE	PS00908. MR_MLE_1. 1 hit. PS00909. MR_MLE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TFDD_DELAC

Accession

Primary (citable) accession number: Q9RNZ9
Secondary accession number(s): Q93T14, Q9WXC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2004
Last modified:	April 3, 2013
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program