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Q9RNZ9 (TFDD_DELAC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chloromuconate cycloisomerase

EC=5.5.1.7
Gene names
Name:tfdD
OrganismDelftia acidovorans (Pseudomonas acidovorans) (Comamonas acidovorans)
Taxonomic identifier80866 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeDelftia

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate.

Cofactor

Manganese By similarity.

Pathway

Aromatic compound metabolism; 3-chlorocatechol degradation.

Induction

By 2,4-dichlorophenoxypropionic acid (2,4-DCPP). Ref.4

Miscellaneous

Different ratios of isoforms are detectable, depending on concentration of dichlorprop.

Sequence similarities

Belongs to the mandelate racemase/muconate lactonizing enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Chloromuconate cycloisomerase
PRO_0000171258

Sites

Active site1651Proton acceptor By similarity
Active site3231Proton donor By similarity
Metal binding1941Manganese By similarity
Metal binding2201Manganese By similarity
Metal binding2451Manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9RNZ9 [UniParc].

Last modified March 1, 2004. Version 2.
Checksum: 195D3101CA18B31C

FASTA37039,465
        10         20         30         40         50         60 
MKIEAISTTI VDVPTRRPLQ MSFTTVHKQS YVIVQVTAGG LVGIGEGGSV GGPTWGSESA 

        70         80         90        100        110        120 
ETIKVIIDNY LAPLLIGKDA SNLSEARALM DRAVTGNLSA KAAIDIALHD LKARALNLSI 

       130        140        150        160        170        180 
ADLIGGTMRK SIPIAWTLAS GDTARDIDSA LEMIEARRHN RFKVKLGART PAQDLEHIRS 

       190        200        210        220        230        240 
IVKAVGDKAS VRVDVNQGWD EQTASIWIPR LEEAGVELVE QPVPRANFGA LRRLTEQNGV 

       250        260        270        280        290        300 
AILADESLSS LSSAFELARD RAVDAFSLKL CNMGGIANTL KVAAIAEAAG ISSYGGTMLD 

       310        320        330        340        350        360 
STVGTAAALH VYATLPSLPY GCELIGPWVL SDRLTQQDLE IKDFEVHLPV GSGLGVDLDH 

       370 
DKVRHYTRAA 

« Hide

References

[1]"A transposon encoding the complete 2,4-dichlorophenoxyacetic acid degradation pathway in the alkalitolerant strain Delftia acidovorans P4a."
Hoffmann D., Kleinsteuber S., Mueller R.H., Babel W.
Microbiology 149:2545-2556(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: P4a.
[2]"Development and application of PCR primers for the detection of the tfd genes in Delftia acidovorans P4a involved in the degradation of 2,4-D."
Hoffmann D., Kleinsteuber S., Mueller R.H., Babel W.
Acta Biotechnol. 21:321-331(2001)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 67-268.
Strain: P4a.
[3]"Physiological and genetic characteristics of two bacterial strains utilizing phenoxypropionate and phenoxyacetate herbicides."
Mueller R.H., Kleinsteuber S., Babel W.
Microbiol. Res. 156:121-131(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-263.
Strain: MC1.
[4]"Regulation of catabolic enzymes during long-term exposure of Delftia acidovorans MC1 to chlorophenoxy herbicides."
Benndorf D., Davidson I., Babel W.
Microbiology 150:1005-1014(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-16, INDUCTION BY 2,4-DCPP.
Strain: MC1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY078159 Genomic DNA. Translation: AAK57008.2.
AF176244 Genomic DNA. Translation: AAD55083.1.
RefSeqYP_006961882.1. NC_019283.1.

3D structure databases

ProteinModelPortalQ9RNZ9.
SMRQ9RNZ9. Positions 1-369.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID13918339.

Enzyme and pathway databases

UniPathwayUPA00083.

Family and domain databases

Gene3D3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProIPR013370. Chloromuconate_cycloisomerase.
IPR029065. Enolase_C-like.
IPR029017. Enolase_N_like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N.
IPR001354. MR_MLE.
[Graphical view]
PANTHERPTHR13794. PTHR13794. 1 hit.
PfamPF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsTIGR02534. mucon_cyclo. 1 hit.
PROSITEPS00908. MR_MLE_1. 1 hit.
PS00909. MR_MLE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTFDD_DELAC
AccessionPrimary (citable) accession number: Q9RNZ9
Secondary accession number(s): Q93T14, Q9WXC9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: June 11, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways