Q9RNU9 (CAPP_STRCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxylase
Short name=PEPC
Short name=PEPCase
EC=4.1.1.31

Gene names

Name:	ppc
Ordered Locus Names:	SCO3127
ORF Names:	SCE66.06c

Organism

Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]

Taxonomic identifier

100226 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces › Streptomyces coelicolor

Protein attributes

Sequence length	911 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle By similarity. HAMAP-Rule MF_00595
Catalytic activity	Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-. HAMAP-Rule MF_00595
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the PEPCase type 1 family.

Ontologies

Keywords
Biological process	Carbon dioxide fixation
Ligand	Magnesium
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	carbon fixation Inferred from electronic annotation. Source: HAMAP oxaloacetate metabolic process Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP phosphoenolpyruvate carboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 911	911	Phosphoenolpyruvate carboxylase HAMAP-Rule MF_00595		PRO_0000166628
Sites
	Active site	137	1	By similarity
	Active site	569	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9RNU9 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 5192E0BB96881273	FASTA	911	101,297
	10 20 30 40 50 60 MSSADDQTTT TTSSELRADI RRLGDLLGET LVRQEGPELL ELVEKVRRLT REDGEAAAEL 70 80 90 100 110 120 LRGTELETAA KLVRAFSTYF HLANVTEQVH RGRELGAKRA AEGGLLARTA DRLKDADPEH 130 140 150 160 170 180 LRETVRNLNV RPVFTAHPTE AARRSVLNKL RRIAALLDTP VNESDRRRLD TRLAENIDLV 190 200 210 220 230 240 WQTDELRVVR PEPADEARNA IYYLDELHLG AVGDVLEDLT AELERAGVKL PDDTRPLTFG 250 260 270 280 290 300 TWIGGDRDGN PNVTPQVTWD VLILQHEHGI NDALEMIDEL RGFLSNSIRY AGATEELLAS 310 320 330 340 350 360 LQADLERLPE ISPRYKRLNA EEPYRLKATC IRQKLENTKQ RLAKGTPHED GRDYLGTAQL 370 380 390 400 410 420 IDDLRIVQTS LREHRGGLFA DGRLARTIRT LAAFGLQLAT MDVREHADAH HHALGQLFDR 430 440 450 460 470 480 LGEESWRYAD MPREYRTKLL AKELRSRRPL APSPAPVDAP GEKTLGVFQT VRRALEVFGP 490 500 510 520 530 540 EVIESYIISM CQGADDVFAA AVLAREAGLI DLHAGWAKIG IVPLLETTDE LKAADTILED 550 560 570 580 590 600 LLADPSYRRL VALRGDVQEV MLGYSDSSKF GGITTSQWEI HRAQRRLRDV AHRYGVRLRL 610 620 630 640 650 660 FHGRGGTVGR GGGPTHDAIL AQPWGTLEGE IKVTEQGEVI SDKYLIPALA RENLELTVAA 670 680 690 700 710 720 TLQASALHTA PRQSDEALAR WDAAMDVVSD AAHTAYRHLV EDPDLPTYFL ASTPVDQLAD 730 740 750 760 770 780 LHLGSRPSRR PGSGVSLDGL RAIPWVFGWT QSRQIVPGWY GVGSGLKALR EAGLDTVLDE 790 800 810 820 830 840 MHQQWHFFRN FISNVEMTLA KTDLRIAQHY VDTLVPDELK HVFDTIKAEH ELTVAEVLRV 850 860 870 880 890 900 TGESELLDAD PVLKQTFTIR DAYLDPISYL QVALLGRQRE AAAANEDPDP LLARALLLTV 910 NGVAAGLRNT G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Phosphoenolpyruvate carboxylase from Streptomyces coelicolor A3(2): purification of the enzyme, cloning of the ppc gene and over-expression of the protein in a streptomycete." Bramwell H., Nimmo H.G., Hunter I.S., Coggins J.R. Biochem. J. 293:131-136(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: A3(2) / NRRL B-16638.
[2]	"The ppc gene of Streptomyces coelicolor A3(2)." Ricketts A.D.J., Hunter I.S. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A3(2) / NRRL B-16638.
[3]	"Characterization of the phosphoenolpyruvate carboxylase gene from Streptomyces coelicolor A3(2) and a ppc gene disruption mutant." Alves A.M.C.R., te Poele E., White J., Bibb M.J., Dijkhuizen L. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A3(2) / NRRL B-16638.
[4]	"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF177946 Genomic DNA. Translation: AAD53311.1. AF160253 Genomic DNA. Translation: AAM54458.1. AL939115 Genomic DNA. Translation: CAB95920.1.
RefSeq	NP_627344.1. NC_003888.3.
3D structure databases
ProteinModelPortal	Q9RNU9.
ModBase	Search...
Protein-protein interaction databases
STRING	100226.SCO3127.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB95920; CAB95920; CAB95920.
GeneID	1098561.
KEGG	sco:SCO3127.
PATRIC	23736002. VBIStrCoe124346_3191.
Phylogenomic databases
eggNOG	COG2352.
HOGENOM	HOG000238647.
KO	K01595.
OMA	AIPWVFG.
ProtClustDB	CLSK902480.
Family and domain databases
HAMAP	MF_00595. PEPcase_type1.
InterPro	IPR021135. PEP_COase. IPR018129. PEP_COase_AS. IPR022805. PEP_COase_bac/pln-type. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Pfam	PF00311. PEPcase. 1 hit. [Graphical view]
PRINTS	PR00150. PEPCARBXLASE.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PROSITE	PS00781. PEPCASE_1. 1 hit. PS00393. PEPCASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAPP_STRCO

Accession

Primary (citable) accession number: Q9RNU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program