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Q9RN37

- USHA_SALPU

UniProt

Q9RN37 - USHA_SALPU

Protein

Protein UshA

Gene

ushA

Organism
Salmonella pullorum
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell.By similarity

    Catalytic activityi

    UDP-sugar + H2O = UMP + alpha-D-aldose 1-phosphate.
    A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

    Cofactori

    Binds 2 zinc ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi41 – 411Zinc 1By similarity
    Metal bindingi43 – 431Zinc 1By similarity
    Metal bindingi84 – 841Zinc 1By similarity
    Metal bindingi84 – 841Zinc 2By similarity
    Metal bindingi116 – 1161Zinc 2By similarity
    Sitei117 – 1171Transition state stabilizerBy similarity
    Sitei120 – 1201Transition state stabilizerBy similarity
    Metal bindingi217 – 2171Zinc 2By similarity
    Metal bindingi252 – 2521Zinc 2By similarity
    Metal bindingi254 – 2541Zinc 1By similarity
    Binding sitei429 – 4291SubstrateBy similarity

    GO - Molecular functioni

    1. 5'-nucleotidase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. nucleotide binding Source: UniProtKB-KW
    4. UDP-sugar diphosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nucleotide catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein UshA
    Including the following 2 domains:
    UDP-sugar hydrolase (EC:3.6.1.45)
    Alternative name(s):
    UDP-sugar diphosphatase
    UDP-sugar pyrophosphatase
    5'-nucleotidase (EC:3.1.3.5)
    Short name:
    5'-NT
    Gene namesi
    Name:ushA
    OrganismiSalmonella pullorum
    Taxonomic identifieri605 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

    Subcellular locationi

    Periplasm By similarity

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525By similarityAdd
    BLAST
    Chaini26 – 550525Protein UshAPRO_0000000032Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi258 ↔ 275By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiQ9RN37.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9RN37.
    SMRiQ9RN37. Positions 26-549.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni498 – 5047Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the 5'-nucleotidase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    3.90.780.10. 1 hit.
    InterProiIPR008334. 5'-Nucleotdase_C.
    IPR006146. 5'-Nucleotdase_CS.
    IPR006179. 5_nucleotidase/apyrase.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view]
    PANTHERiPTHR11575. PTHR11575. 1 hit.
    PfamiPF02872. 5_nucleotid_C. 1 hit.
    PF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR01607. APYRASEFAMLY.
    SUPFAMiSSF55816. SSF55816. 1 hit.
    SSF56300. SSF56300. 1 hit.
    PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
    PS00786. 5_NUCLEOTIDASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9RN37-1 [UniParc]FASTAAdd to Basket

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    MKFLKRGVAL ALLAAFALTT QPAQAYEKDK TYKITILHTN DHHGHFWRSE    50
    YGEYGLAAQK TLVDSIRKEV AQEGGGVLLL SGGDINTGVP ESDLQDAEPD 100
    FRGMNLIGYD AMAVGNHEFD NPLTVLRQQE KWAKFPFLSA NIYQKSTGER 150
    LFKPWAIFTR QDIKIAVIGL TTDDTAKIGN PEYFTDIEFR KPAEEAKVVI 200
    QELNMNEKPD VIIATTHMGH YDNGDHGSNA PGDVEMARSL PAGSLAMIVG 250
    GHSQDPVCMA SENKKQVNYV PGTPCAPDKQ NGIWIVQAHE WGKYVGRADF 300
    EFRNGEMKMV NYQLIPVNLK KKVTWDNGKS ERVLYTPEIA ENPQMLLLLT 350
    PFQNKGKVQL EVKIGSVNGL LEGDRSKVRF VQTNMGRVIL AAQIARTGAD 400
    FGVMSGGGIR DSIEAGDITY KSVLKVQPFG NIVVYADMSG KEVVDYLTRV 450
    AQMKPDSGAY PQLANVSFVA KEGKLTDLKI KGEPVDPAKT YRMATLSFNA 500
    TGGDGYPRID NKPGYVNTGF IDAEVLKEFI QQNSPLDAAA FTPKGEVSWL 550
    Length:550
    Mass (Da):60,604
    Last modified:May 1, 2000 - v1
    Checksum:i655BC7E8F0D4F62F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF188727 Genomic DNA. Translation: AAF05581.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF188727 Genomic DNA. Translation: AAF05581.1 .

    3D structure databases

    ProteinModelPortali Q9RN37.
    SMRi Q9RN37. Positions 26-549.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9RN37.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    3.90.780.10. 1 hit.
    InterProi IPR008334. 5'-Nucleotdase_C.
    IPR006146. 5'-Nucleotdase_CS.
    IPR006179. 5_nucleotidase/apyrase.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view ]
    PANTHERi PTHR11575. PTHR11575. 1 hit.
    Pfami PF02872. 5_nucleotid_C. 1 hit.
    PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR01607. APYRASEFAMLY.
    SUPFAMi SSF55816. SSF55816. 1 hit.
    SSF56300. SSF56300. 1 hit.
    PROSITEi PS00785. 5_NUCLEOTIDASE_1. 1 hit.
    PS00786. 5_NUCLEOTIDASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The cryptic ushA gene (ushA(c)) in natural isolates of Salmonella enterica (serotype Typhimurium) has been inactivated by a single missense mutation."
      Innes D., Beacham I.R., Beven C.-A., Douglas M., Laird M.W., Joly J.C., Burns D.M.
      Microbiology 147:1887-1896(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiUSHA_SALPU
    AccessioniPrimary (citable) accession number: Q9RN37
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2003
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3