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Q9RN37

- USHA_SALPU

UniProt

Q9RN37 - USHA_SALPU

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Protein

Protein UshA

Gene

ushA

Organism
Salmonella pullorum
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell.By similarity

Catalytic activityi

UDP-sugar + H2O = UMP + alpha-D-aldose 1-phosphate.
A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn(2+) ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi41 – 411Zinc 1By similarity
Metal bindingi43 – 431Zinc 1By similarity
Metal bindingi84 – 841Zinc 1By similarity
Metal bindingi84 – 841Zinc 2By similarity
Metal bindingi116 – 1161Zinc 2By similarity
Sitei117 – 1171Transition state stabilizerBy similarity
Sitei120 – 1201Transition state stabilizerBy similarity
Metal bindingi217 – 2171Zinc 2By similarity
Metal bindingi252 – 2521Zinc 2By similarity
Metal bindingi254 – 2541Zinc 1By similarity
Binding sitei429 – 4291SubstrateBy similarity

GO - Molecular functioni

  1. 5'-nucleotidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. nucleotide binding Source: UniProtKB-KW
  4. UDP-sugar diphosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. nucleotide catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein UshA
Including the following 2 domains:
UDP-sugar hydrolase (EC:3.6.1.45)
Alternative name(s):
UDP-sugar diphosphatase
UDP-sugar pyrophosphatase
5'-nucleotidase (EC:3.1.3.5)
Short name:
5'-NT
Gene namesi
Name:ushA
OrganismiSalmonella pullorum
Taxonomic identifieri605 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Subcellular locationi

Periplasm By similarity

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525By similarityAdd
BLAST
Chaini26 – 550525Protein UshAPRO_0000000032Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi258 ↔ 275By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ9RN37.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9RN37.
SMRiQ9RN37. Positions 26-549.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni498 – 5047Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9RN37-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFLKRGVAL ALLAAFALTT QPAQAYEKDK TYKITILHTN DHHGHFWRSE
60 70 80 90 100
YGEYGLAAQK TLVDSIRKEV AQEGGGVLLL SGGDINTGVP ESDLQDAEPD
110 120 130 140 150
FRGMNLIGYD AMAVGNHEFD NPLTVLRQQE KWAKFPFLSA NIYQKSTGER
160 170 180 190 200
LFKPWAIFTR QDIKIAVIGL TTDDTAKIGN PEYFTDIEFR KPAEEAKVVI
210 220 230 240 250
QELNMNEKPD VIIATTHMGH YDNGDHGSNA PGDVEMARSL PAGSLAMIVG
260 270 280 290 300
GHSQDPVCMA SENKKQVNYV PGTPCAPDKQ NGIWIVQAHE WGKYVGRADF
310 320 330 340 350
EFRNGEMKMV NYQLIPVNLK KKVTWDNGKS ERVLYTPEIA ENPQMLLLLT
360 370 380 390 400
PFQNKGKVQL EVKIGSVNGL LEGDRSKVRF VQTNMGRVIL AAQIARTGAD
410 420 430 440 450
FGVMSGGGIR DSIEAGDITY KSVLKVQPFG NIVVYADMSG KEVVDYLTRV
460 470 480 490 500
AQMKPDSGAY PQLANVSFVA KEGKLTDLKI KGEPVDPAKT YRMATLSFNA
510 520 530 540 550
TGGDGYPRID NKPGYVNTGF IDAEVLKEFI QQNSPLDAAA FTPKGEVSWL
Length:550
Mass (Da):60,604
Last modified:May 1, 2000 - v1
Checksum:i655BC7E8F0D4F62F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF188727 Genomic DNA. Translation: AAF05581.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF188727 Genomic DNA. Translation: AAF05581.1 .

3D structure databases

ProteinModelPortali Q9RN37.
SMRi Q9RN37. Positions 26-549.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9RN37.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProi IPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view ]
PANTHERi PTHR11575. PTHR11575. 1 hit.
Pfami PF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR01607. APYRASEFAMLY.
SUPFAMi SSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEi PS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The cryptic ushA gene (ushA(c)) in natural isolates of Salmonella enterica (serotype Typhimurium) has been inactivated by a single missense mutation."
    Innes D., Beacham I.R., Beven C.-A., Douglas M., Laird M.W., Joly J.C., Burns D.M.
    Microbiology 147:1887-1896(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiUSHA_SALPU
AccessioniPrimary (citable) accession number: Q9RN37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: May 1, 2000
Last modified: November 26, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3