SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9RN37

- USHA_SALPU

UniProt

Q9RN37 - USHA_SALPU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Protein UshA

Gene
ushA
Organism
Salmonella pullorum
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell By similarity.

Catalytic activityi

UDP-sugar + H2O = UMP + alpha-D-aldose 1-phosphate.
A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Binds 2 zinc ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi41 – 411Zinc 1 By similarity
Metal bindingi43 – 431Zinc 1 By similarity
Metal bindingi84 – 841Zinc 1 By similarity
Metal bindingi84 – 841Zinc 2 By similarity
Metal bindingi116 – 1161Zinc 2 By similarity
Sitei117 – 1171Transition state stabilizer By similarity
Sitei120 – 1201Transition state stabilizer By similarity
Metal bindingi217 – 2171Zinc 2 By similarity
Metal bindingi252 – 2521Zinc 2 By similarity
Metal bindingi254 – 2541Zinc 1 By similarity
Binding sitei429 – 4291Substrate By similarity

GO - Molecular functioni

  1. 5'-nucleotidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. nucleotide binding Source: UniProtKB-KW
  4. UDP-sugar diphosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. nucleotide catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein UshA
Including the following 2 domains:
UDP-sugar hydrolase (EC:3.6.1.45)
Alternative name(s):
UDP-sugar diphosphatase
UDP-sugar pyrophosphatase
5'-nucleotidase (EC:3.1.3.5)
Short name:
5'-NT
Gene namesi
Name:ushA
OrganismiSalmonella pullorum
Taxonomic identifieri605 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Subcellular locationi

Periplasm By similarity

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 By similarityAdd
BLAST
Chaini26 – 550525Protein UshAPRO_0000000032Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi258 ↔ 275 By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ9RN37.

Interactioni

Subunit structurei

Monomer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9RN37.
SMRiQ9RN37. Positions 26-549.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni498 – 5047Substrate binding By similarity

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9RN37-1 [UniParc]FASTAAdd to Basket

« Hide

MKFLKRGVAL ALLAAFALTT QPAQAYEKDK TYKITILHTN DHHGHFWRSE    50
YGEYGLAAQK TLVDSIRKEV AQEGGGVLLL SGGDINTGVP ESDLQDAEPD 100
FRGMNLIGYD AMAVGNHEFD NPLTVLRQQE KWAKFPFLSA NIYQKSTGER 150
LFKPWAIFTR QDIKIAVIGL TTDDTAKIGN PEYFTDIEFR KPAEEAKVVI 200
QELNMNEKPD VIIATTHMGH YDNGDHGSNA PGDVEMARSL PAGSLAMIVG 250
GHSQDPVCMA SENKKQVNYV PGTPCAPDKQ NGIWIVQAHE WGKYVGRADF 300
EFRNGEMKMV NYQLIPVNLK KKVTWDNGKS ERVLYTPEIA ENPQMLLLLT 350
PFQNKGKVQL EVKIGSVNGL LEGDRSKVRF VQTNMGRVIL AAQIARTGAD 400
FGVMSGGGIR DSIEAGDITY KSVLKVQPFG NIVVYADMSG KEVVDYLTRV 450
AQMKPDSGAY PQLANVSFVA KEGKLTDLKI KGEPVDPAKT YRMATLSFNA 500
TGGDGYPRID NKPGYVNTGF IDAEVLKEFI QQNSPLDAAA FTPKGEVSWL 550
Length:550
Mass (Da):60,604
Last modified:May 1, 2000 - v1
Checksum:i655BC7E8F0D4F62F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF188727 Genomic DNA. Translation: AAF05581.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF188727 Genomic DNA. Translation: AAF05581.1 .

3D structure databases

ProteinModelPortali Q9RN37.
SMRi Q9RN37. Positions 26-549.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9RN37.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProi IPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view ]
PANTHERi PTHR11575. PTHR11575. 1 hit.
Pfami PF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR01607. APYRASEFAMLY.
SUPFAMi SSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEi PS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The cryptic ushA gene (ushA(c)) in natural isolates of Salmonella enterica (serotype Typhimurium) has been inactivated by a single missense mutation."
    Innes D., Beacham I.R., Beven C.-A., Douglas M., Laird M.W., Joly J.C., Burns D.M.
    Microbiology 147:1887-1896(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiUSHA_SALPU
AccessioniPrimary (citable) accession number: Q9RN37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi