Q9RLV9 (PHLC_LISIV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sphingomyelinase C
Short name=SMase
EC=3.1.4.12

Alternative name(s):
Sphingomyelin phosphodiesterase

Gene names

Name:

smcL

Organism

Listeria ivanovii

Taxonomic identifier

1638 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Virulence factor that promotes intracellular proliferation by mediating the disruption of the phagocytic vacuole and the release of bacteria into the host cell cytosol. May act in concert with the phospholipases plcA and plcB and the hemolysin hly to mediate efficient escape from the vacuole. Ref.1
Catalytic activity	Sphingomyelin + H2O = N-acylsphingosine + choline phosphate.
Subcellular location	Secreted.
Disruption phenotype	A knockout mutant was shown to be weakly hemolytic on sheep blood agar, does not produce the external halo of incomplete hemolysis characteristic of L.ivanovii and does not give the typical shovel-shaped co-operative hemolytic "CAMP-like" reaction that happens with Rhodococcus equi. Is also much less virulent for mice infected intravenously. Ref.1
Miscellaneous	Complementation with smcL of a mutant lacking the membrane-damaging determinants hly, plcA and plcB (thus unable to grow intracellularly) was sufficient to promote bacterial intracellular proliferation.
Sequence similarities	Belongs to the neutral sphingomyelinase family.

Ontologies

Keywords
Biological process	Cytolysis Hemolysis Virulence
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW hemolysis in other organism Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sphingomyelin phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 28

28

Potential

Chain

29 – 335

307

Sphingomyelinase C

PRO_0000046031

Secondary structure

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335

Helix Strand Turn

Details...

Beta strand

46 – 54

9

Turn

57 – 59

3

Helix

65 – 73

9

Helix

76 – 78

3

Beta strand

82 – 89

8

Helix

92 – 101

10

Turn

102 – 105

4

Beta strand

121 – 124

4

Beta strand

138 – 143

6

Beta strand

145 – 152

8

Helix

158 – 162

5

Beta strand

166 – 174

9

Beta strand

177 – 185

9

Helix

195 – 216

22

Beta strand

222 – 229

8

Helix

237 – 246

10

Turn

263 – 265

3

Helix

267 – 272

6

Beta strand

282 – 287

6

Beta strand

296 – 300

5

Beta strand

308 – 312

5

Beta strand

315 – 319

5

Beta strand

322 – 325

4

Beta strand

328 – 332

5

Sequences

	Sequence		Length	Mass (Da)
	Q9RLV9 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 46F398E58A8434D7	FASTA	335	38,455
	10 20 30 40 50 60 MEKFKIIKTI PKICGAFIFL LFFTFLFGHY GELKTQASDE YPGNFKITSH NVYLFSRNIY 70 80 90 100 110 120 PNWGQMHRAD LIAQADYMKN NDVVILNEAF DTSASHRLLN NLREMYPHQT PVIGRSKHGW 130 140 150 160 170 180 DKTEGNYSNF ALEDGGVAVV SQWPIVEKSQ HIFQRGGGAD RLSNKGFAYV KIMKNGKPYH 190 200 210 220 230 240 IIGTHTPADD SLISKDTSRA IRAEQMQEIQ TFIAKKNIPK DEIIFIGGDL NVNYGTDEYH 250 260 270 280 290 300 DMFKLLNVSS PANFNGQMAT WDPTTNSMLK ESYPKAAPEY LDYIFVENGH ARPHSWHNKV 310 320 330 LHTKSPQWSV KSWFKTYTYQ DFSDHYPVVG FTDNN

References

[1]

"The smcL gene of Listeria ivanovii encodes a sphingomyelinase C that mediates bacterial escape from the phagocytic vacuole." Gonzalez-Zorn B., Dominguez-Bernal G., Suarez M., Ripio M.-T., Vega Y., Novella S., Vazquez-Boland J.-A. Mol. Microbiol. 33:510-523(1999) [PubMed: 10417642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE. Strain: ATCC 19119 / DSM 20750 / JCM 7681 / NCTC 11846 / SLCC 2379.

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Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y09477 Genomic DNA. Translation: CAA70683.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZWX	X-ray	1.90	A	36-335	[»]

ProteinModelPortal

Q9RLV9.

SMR

Q9RLV9. Positions 41-333.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BRENDA

3.1.4.12. 8286.

Family and domain databases

InterPro

IPR005135. Endo/exonuclease/phosphatase.
IPR017766. Sphingomyelinase/PLipase_C.
[Graphical view]

Pfam

PF03372. Exo_endo_phos. 1 hit.
[Graphical view]

SUPFAM

SSF56219. Exo_endo_phos. 1 hit.

TIGRFAMs

TIGR03395. Sphingomy. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PHLC_LISIV

Accession

Primary (citable) accession number: Q9RLV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	May 1, 2000
Last modified:	October 19, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program