Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9RLU5

- ALR_LACLM

UniProt

Q9RLU5 - ALR_LACLM

Protein

Alanine racemase

Gene

alr

Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (05 Mar 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.UniRule annotation

    Catalytic activityi

    L-alanine = D-alanine.UniRule annotation

    Cofactori

    Pyridoxal phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei40 – 401Proton acceptor; specific for D-alanineUniRule annotation
    Binding sitei136 – 1361SubstrateUniRule annotation
    Active sitei263 – 2631Proton acceptor; specific for L-alanineUniRule annotation
    Binding sitei310 – 3101Substrate; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. alanine racemase activity Source: UniProtKB-HAMAP
    2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. D-alanine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciLLAC416870:GCDT-1711-MONOMER.
    UniPathwayiUPA00042; UER00497.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine racemaseUniRule annotation (EC:5.1.1.1UniRule annotation)
    Gene namesi
    Name:alr
    Ordered Locus Names:llmg_1704
    OrganismiLactococcus lactis subsp. cremoris (strain MG1363)
    Taxonomic identifieri416870 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
    ProteomesiUP000000364: Chromosome

    Pathology & Biotechi

    Biotechnological usei

    Homoalanine fermentation combined with the disruption of the alr gene allowed the industrial and stereospecific production (>99%) of L-alanine, which is used as a food sweetener and for pharmaceutical applications.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367Alanine racemasePRO_0000114527Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei40 – 401N6-(pyridoxal phosphate)lysineUniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi416870.llmg_1704.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9RLU5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alanine racemase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0787.
    HOGENOMiHOG000031444.
    KOiK01775.
    OMAiIYSHLAL.
    OrthoDBiEOG6PP9NJ.

    Family and domain databases

    Gene3Di2.40.37.10. 1 hit.
    3.20.20.10. 1 hit.
    HAMAPiMF_01201. Ala_racemase.
    InterProiIPR000821. Ala_racemase.
    IPR009006. Ala_racemase/Decarboxylase_C.
    IPR011079. Ala_racemase_C.
    IPR001608. Ala_racemase_N.
    IPR020622. Ala_racemase_pyridoxalP-BS.
    IPR029066. PLP-binding_barrel.
    [Graphical view]
    PfamiPF00842. Ala_racemase_C. 1 hit.
    PF01168. Ala_racemase_N. 1 hit.
    [Graphical view]
    PRINTSiPR00992. ALARACEMASE.
    SMARTiSM01005. Ala_racemase_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF50621. SSF50621. 1 hit.
    SSF51419. SSF51419. 1 hit.
    TIGRFAMsiTIGR00492. alr. 1 hit.
    PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9RLU5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTSPHRNTS AIVDLKAIRN NIEKFKKHIN PNAEIWPAVK ADAYGHGSIE    50
    VSKAVSDLVG GFCVSNLDEA IELRNHLVTK PILVLSGIVP EDVDIAAALN 100
    ISLTAPSLEW LKLVVQEEAE LSDLKIHIGV DSGMGRIGIR DVEEANQMIE 150
    LADKYAINFE GIFTHFATAD MADETKFKNQ QARFNKIMAG LSRQPKFIHS 200
    TNTAAALWHK EQVQAIERLG ISMYGLNPSG KTLELPFEIE PALSLVSELT 250
    HIKKIAAGET VGYGATYETS EETWIGTVPI GYADGWTRQM QGFKVLVDGK 300
    FCEIVGRVCM DQMMIKLDKS YPLGTKVTLI GRDKANEITT TDVADWRGTI 350
    NYEVLCLLSD RIKRIYK 367
    Length:367
    Mass (Da):40,677
    Last modified:March 5, 2002 - v2
    Checksum:iB6DE1496315FA814
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y18148 Genomic DNA. Translation: CAB56755.2.
    AM406671 Genomic DNA. Translation: CAL98277.1.
    RefSeqiYP_001032982.1. NC_009004.1.

    Genome annotation databases

    EnsemblBacteriaiCAL98277; CAL98277; llmg_1704.
    GeneIDi4796695.
    KEGGillm:llmg_1704.
    PATRICi22284585. VBILacLac4574_1747.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y18148 Genomic DNA. Translation: CAB56755.2 .
    AM406671 Genomic DNA. Translation: CAL98277.1 .
    RefSeqi YP_001032982.1. NC_009004.1.

    3D structure databases

    ProteinModelPortali Q9RLU5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 416870.llmg_1704.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL98277 ; CAL98277 ; llmg_1704 .
    GeneIDi 4796695.
    KEGGi llm:llmg_1704.
    PATRICi 22284585. VBILacLac4574_1747.

    Phylogenomic databases

    eggNOGi COG0787.
    HOGENOMi HOG000031444.
    KOi K01775.
    OMAi IYSHLAL.
    OrthoDBi EOG6PP9NJ.

    Enzyme and pathway databases

    UniPathwayi UPA00042 ; UER00497 .
    BioCyci LLAC416870:GCDT-1711-MONOMER.

    Family and domain databases

    Gene3Di 2.40.37.10. 1 hit.
    3.20.20.10. 1 hit.
    HAMAPi MF_01201. Ala_racemase.
    InterProi IPR000821. Ala_racemase.
    IPR009006. Ala_racemase/Decarboxylase_C.
    IPR011079. Ala_racemase_C.
    IPR001608. Ala_racemase_N.
    IPR020622. Ala_racemase_pyridoxalP-BS.
    IPR029066. PLP-binding_barrel.
    [Graphical view ]
    Pfami PF00842. Ala_racemase_C. 1 hit.
    PF01168. Ala_racemase_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00992. ALARACEMASE.
    SMARTi SM01005. Ala_racemase_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50621. SSF50621. 1 hit.
    SSF51419. SSF51419. 1 hit.
    TIGRFAMsi TIGR00492. alr. 1 hit.
    PROSITEi PS00395. ALANINE_RACEMASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Conversion of Lactococcus lactis from homolactic to homoalanine fermentation through metabolic engineering."
      Hols P., Kleerebezem M., Schank A.N., Ferain T., Hugenholtz J., Delcour J., De Vos W.M.
      Nat. Biotechnol. 17:588-592(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363."
      Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.
      J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MG1363.

    Entry informationi

    Entry nameiALR_LACLM
    AccessioniPrimary (citable) accession number: Q9RLU5
    Secondary accession number(s): A2RLV4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 5, 2002
    Last sequence update: March 5, 2002
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3