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Q9RLU5

- ALR_LACLM

UniProt

Q9RLU5 - ALR_LACLM

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Protein

Alanine racemase

Gene
alr, llmg_1704
Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids By similarity.UniRule annotation

Catalytic activityi

L-alanine = D-alanine.UniRule annotation

Cofactori

Pyridoxal phosphate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401Proton acceptor; specific for D-alanine By similarity
Binding sitei136 – 1361Substrate By similarity
Active sitei263 – 2631Proton acceptor; specific for L-alanine By similarity
Binding sitei310 – 3101Substrate; via amide nitrogen By similarity

GO - Molecular functioni

  1. alanine racemase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. D-alanine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciLLAC416870:GCDT-1711-MONOMER.
UniPathwayiUPA00042; UER00497.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemase (EC:5.1.1.1)
Gene namesi
Name:alr
Ordered Locus Names:llmg_1704
OrganismiLactococcus lactis subsp. cremoris (strain MG1363)
Taxonomic identifieri416870 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000000364: Chromosome

Pathology & Biotechi

Biotechnological usei

Homoalanine fermentation combined with the disruption of the alr gene allowed the industrial and stereospecific production (>99%) of L-alanine, which is used as a food sweetener and for pharmaceutical applications.UniRule annotation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 367367Alanine racemaseUniRule annotationPRO_0000114527Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei40 – 401N6-(pyridoxal phosphate)lysine By similarity

Interactioni

Protein-protein interaction databases

STRINGi416870.llmg_1704.

Structurei

3D structure databases

ProteinModelPortaliQ9RLU5.

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.

Phylogenomic databases

eggNOGiCOG0787.
HOGENOMiHOG000031444.
KOiK01775.
OMAiIYSHLAL.
OrthoDBiEOG6PP9NJ.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9RLU5-1 [UniParc]FASTAAdd to Basket

« Hide

MKTSPHRNTS AIVDLKAIRN NIEKFKKHIN PNAEIWPAVK ADAYGHGSIE    50
VSKAVSDLVG GFCVSNLDEA IELRNHLVTK PILVLSGIVP EDVDIAAALN 100
ISLTAPSLEW LKLVVQEEAE LSDLKIHIGV DSGMGRIGIR DVEEANQMIE 150
LADKYAINFE GIFTHFATAD MADETKFKNQ QARFNKIMAG LSRQPKFIHS 200
TNTAAALWHK EQVQAIERLG ISMYGLNPSG KTLELPFEIE PALSLVSELT 250
HIKKIAAGET VGYGATYETS EETWIGTVPI GYADGWTRQM QGFKVLVDGK 300
FCEIVGRVCM DQMMIKLDKS YPLGTKVTLI GRDKANEITT TDVADWRGTI 350
NYEVLCLLSD RIKRIYK 367
Length:367
Mass (Da):40,677
Last modified:March 5, 2002 - v2
Checksum:iB6DE1496315FA814
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y18148 Genomic DNA. Translation: CAB56755.2.
AM406671 Genomic DNA. Translation: CAL98277.1.
RefSeqiYP_001032982.1. NC_009004.1.

Genome annotation databases

EnsemblBacteriaiCAL98277; CAL98277; llmg_1704.
GeneIDi4796695.
KEGGillm:llmg_1704.
PATRICi22284585. VBILacLac4574_1747.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y18148 Genomic DNA. Translation: CAB56755.2 .
AM406671 Genomic DNA. Translation: CAL98277.1 .
RefSeqi YP_001032982.1. NC_009004.1.

3D structure databases

ProteinModelPortali Q9RLU5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 416870.llmg_1704.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL98277 ; CAL98277 ; llmg_1704 .
GeneIDi 4796695.
KEGGi llm:llmg_1704.
PATRICi 22284585. VBILacLac4574_1747.

Phylogenomic databases

eggNOGi COG0787.
HOGENOMi HOG000031444.
KOi K01775.
OMAi IYSHLAL.
OrthoDBi EOG6PP9NJ.

Enzyme and pathway databases

UniPathwayi UPA00042 ; UER00497 .
BioCyci LLAC416870:GCDT-1711-MONOMER.

Family and domain databases

Gene3Di 2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPi MF_01201. Ala_racemase.
InterProi IPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view ]
Pfami PF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view ]
PRINTSi PR00992. ALARACEMASE.
SMARTi SM01005. Ala_racemase_C. 1 hit.
[Graphical view ]
SUPFAMi SSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsi TIGR00492. alr. 1 hit.
PROSITEi PS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Conversion of Lactococcus lactis from homolactic to homoalanine fermentation through metabolic engineering."
    Hols P., Kleerebezem M., Schank A.N., Ferain T., Hugenholtz J., Delcour J., De Vos W.M.
    Nat. Biotechnol. 17:588-592(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363."
    Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.
    J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MG1363.

Entry informationi

Entry nameiALR_LACLM
AccessioniPrimary (citable) accession number: Q9RLU5
Secondary accession number(s): A2RLV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 5, 2002
Last modified: June 11, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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