Reviewed, UniProtKB/Swiss-Prot Q9RKL4 (PUR2_STRCO)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	SCO4068
ORF Names:	SCD25.04

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151486
Regions
	Domain	107 – 303	197	ATP-grasp
	Nucleotide binding	133 – 184	52	ATP By similarity
Sites
	Metal binding	273	1	Magnesium or manganese By similarity
	Metal binding	275	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9RKL4-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 21715503DEEF70B3	FASTA	416	42,623
	10 20 30 40 50 60 MKVLVIGGGA REHALCRSLS LDPDVTALHC APGNAGIAEV AELHQVDALD GAAVTALAGR 70 80 90 100 110 120 LGAELVVVGP EAPLVAGVAD AVREAGIPVF GPSGEAARLE GSKAFAKDVM ACAGVPTARS 130 140 150 160 170 180 YVCTNPAEVD AALAAFGAPY VVKDDGLAAG KGVVVTDDVE AARAHANACD RVVVEEFLDG 190 200 210 220 230 240 PEVSLFAVTD GENVRPLQPA QDFKRALDGD EGPNTGGMGA YSPLPWADPK LVDEVVQSVL 250 260 270 280 290 300 QPTVDEMRRR GTPFSGLLYA GLAITSRGVR VIEFNARFGD PETQVVLARL KTPLAGLLMA 310 320 330 340 350 360 AATGNLADLE PLRWSDEAAV TVVVASHNYP GTPRTGDPIT GLDEVAAQDA PHAYVLHAGT 370 380 390 400 410 RAEGDAVVSA GGRVLSVTAT GADLTEARDR AYRAVARIGL DGSQHRTDIA AKAAGA

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL939118 Genomic DNA. Translation: CAB56348.1.
RefSeq	NP_628249.1.
3D structure databases
HSSP	HSSP built from PDB template 1GSO based on UniProtKB P15640.
ModBase	Search...
Genome annotation databases
GeneID	1099505.
GenomeReviews	Gene locus SCO4068 in contig AL645882_GR.
KEGG	sco:SCO4068.
NMPDR	fig|100226.1.peg.4024.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9RKL4.
OMA	IEYNCRF.
Enzyme and pathway databases
BioCyc	SCOE100226:SCO4068-MON.
BRENDA	6.3.4.13. 1084.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_STRCO

Accession

Primary (citable) accession number: Q9RKL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)