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Q9RKL4 (PUR2_STRCO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:SCO4068
ORF Names:SCD25.04
OrganismStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]
Taxonomic identifier100226 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group

Protein attributes

Sequence length416 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 416416Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151486

Regions

Domain107 – 303197ATP-grasp
Nucleotide binding133 – 18452ATP By similarity

Sites

Metal binding2731Magnesium or manganese By similarity
Metal binding2751Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9RKL4 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 21715503DEEF70B3

FASTA41642,623
        10         20         30         40         50         60 
MKVLVIGGGA REHALCRSLS LDPDVTALHC APGNAGIAEV AELHQVDALD GAAVTALAGR 

        70         80         90        100        110        120 
LGAELVVVGP EAPLVAGVAD AVREAGIPVF GPSGEAARLE GSKAFAKDVM ACAGVPTARS 

       130        140        150        160        170        180 
YVCTNPAEVD AALAAFGAPY VVKDDGLAAG KGVVVTDDVE AARAHANACD RVVVEEFLDG 

       190        200        210        220        230        240 
PEVSLFAVTD GENVRPLQPA QDFKRALDGD EGPNTGGMGA YSPLPWADPK LVDEVVQSVL 

       250        260        270        280        290        300 
QPTVDEMRRR GTPFSGLLYA GLAITSRGVR VIEFNARFGD PETQVVLARL KTPLAGLLMA 

       310        320        330        340        350        360 
AATGNLADLE PLRWSDEAAV TVVVASHNYP GTPRTGDPIT GLDEVAAQDA PHAYVLHAGT 

       370        380        390        400        410 
RAEGDAVVSA GGRVLSVTAT GADLTEARDR AYRAVARIGL DGSQHRTDIA AKAAGA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL939118 Genomic DNA. Translation: CAB56348.1.
RefSeqNP_628249.1. NC_003888.3.

3D structure databases

ProteinModelPortalQ9RKL4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING100226.SCO4068.

Proteomic databases

PRIDEQ9RKL4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB56348; CAB56348; CAB56348.
GeneID1099505.
KEGGsco:SCO4068.
PATRIC23737902. VBIStrCoe124346_4127.

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMAYKGFLYA.
OrthoDBEOG69SKD1.
PhylomeDBQ9RKL4.

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_STRCO
AccessionPrimary (citable) accession number: Q9RKL4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: May 1, 2000
Last modified: May 14, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways