Beta-mannosidase - Thermotoga maritima

Preferred order of sections

Names and origin

Protein names

Submitted name:
Beta-mannosidase EMBL CAB56854.1

Gene names

Name:

manB EMBL CAB56854.1

Organism

Thermotoga maritima EMBL CAB56854.1

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	680 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Calcium PDB 1OF3 PDB 1OF4 PDB 1OH4 Metal-binding PDB 1OF3 PDB 1OF4 PDB 1OH4
Technical term	3D-structure PDB 1OF3 PDB 1OF4 PDB 1OH4
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Region

563 – 568

6

Beta-D-mannose binding PDB 1OF4 PDB 1OH4

Region

608 – 610

3

Beta-D-mannose binding PDB 1OF4 PDB 1OH4

Sites

Metal binding

516

1

Calcium; via carbonyl oxygen PDB 1OF3 PDB 1OF4 PDB 1OH4

Metal binding

548

1

Calcium; via carbonyl oxygen PDB 1OF3 PDB 1OF4 PDB 1OH4

Metal binding

550

1

Calcium; via carbonyl oxygen PDB 1OF3 PDB 1OF4 PDB 1OH4

Metal binding

669

1

Calcium PDB 1OF3 PDB 1OF4 PDB 1OH4

Binding site

599

1

Beta-D-mannose PDB 1OF4 PDB 1OH4

Binding site

614

1

Beta-D-mannose PDB 1OF4 PDB 1OH4

Sequences

Sequence

Length

Mass (Da)

Tools

Q9RIK9 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 5BA3F6982194D1AA
Show »

FASTA

680

77,687

        10         20         30         40         50         60 
MGIGGDDSWS PSVSAEFLLL IVELSFVLFA SDEFVKVENG KFALNGKEFR FIGSNNYYMH 

        70         80         90        100        110        120 
YKSNGMIDSV LESARDMGIK VLRIWGFLDG ESYCRDKNTY MHPEPGVFGV PEGISNAQSG 

       130        140        150        160        170        180 
FERLDYTVAK AKELGIKLVI VLVNNWDDFG GMNQYVRWFG GTHHDDFYRD EKIKEEYKKY 

       190        200        210        220        230        240 
VSFLVNHVNT YTGVPYREEP TIMAWELANE PRCETDKSGN TLVEWVKEMS SYIKSLDPNH 

       250        260        270        280        290        300 
LVAVGDEGFF SNYEGFKPYG GEAEWAYNGW SGVDWKKLLS IETVDFGTFH LYPSHWGVSP 

       310        320        330        340        350        360 
ENYAQWGAKW IEDHIKIAKE IGKPVVLEEY GIPKSAPVNR TAIYRLWNDL VYDLGGDGAM 

       370        380        390        400        410        420 
FWMLAGIGEG SDRDERGYYP DYDGFRIVND DSPEAELIRE YAKLFNTGED IREDTCSFIL 

       430        440        450        460        470        480 
PKDGMEIKKT VEVRAGVFDY SNTFEKLSVK VEDLVFENEI EHLGYGIYGF DLDTTRIPDG 

       490        500        510        520        530        540 
EHEMFLEGHF QGKTVKDSIK AKVVNEARYV LAEEVDFSSP EEVKNWWNSG TWQAEFGSPD 

       550        560        570        580        590        600 
IEWNGEVGNG ALQLNVKLPG KSDWEEVRVA RKFERLSECE ILEYDIYIPN VEGLKGRLRP 

       610        620        630        640        650        660 
YAVLNPGWVK IGLDMNNANV ESAEIITFGG KEYRRFHVRI EFDRTAGVKE LHIGVVGDHL 

       670        680 
RYDGPIFIDN VRLYKRTGGM

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References

[1]	"Amino Acid Sequence of beta-mannosidase genes from the hyperthermophilic bacteria Thermotoga maritima and Thermotoga neapolitana." Parker K.N., Lam D., Duffaud G., Snead M.A., Mathur E.J., Kelly R.M. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: MSB8 EMBL CAB56854.1.
[2]	"Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate binding module, TmCBM27." Boraston A.B., Revett T.J., Boraston C.M., Nurizzo D., Davies G.J. Structure 11:665-675(2003) [PubMed: 12791255] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF 505-680 IN COMPLEX WITH BETA-D-MANNOSE AND CALCIUM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y17980 Genomic DNA. Translation: CAB56854.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OF3	X-ray	2.00	A/B	505-680	[»]
1OF4	X-ray	1.60	A	505-680	[»]
1OH4	X-ray	1.35	A	505-680	[»]

ProteinModelPortal

Q9RIK9.

SMR

Q9RIK9. Positions 507-680.

ModBase

Search...

Protein family/group databases

CAZy

CBM27. Carbohydrate-Binding Module Family 27.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR015295. Carb-bd_module_27.
IPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.

Pfam

PF09212. CBM27. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]

SUPFAM

SSF49785. Gal_bind_like. 1 hit.
SSF51445. Glyco_hydro_cat. 1 hit.

ProtoNet

Search...

Entry information

Entry name

Q9RIK9_THEMA

Accession

Primary (citable) accession number: Q9RIK9

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 2000
Last sequence update:	May 1, 2000
Last modified:	September 21, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)