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Q9RHX4 (PUR2_CORAM) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
OrganismCorynebacterium ammoniagenes (Brevibacterium ammoniagenes)
Taxonomic identifier1697 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length426 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 426426Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151445

Regions

Domain109 – 312204ATP-grasp
Nucleotide binding138 – 19356ATP By similarity

Sites

Metal binding2821Magnesium or manganese By similarity
Metal binding2841Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9RHX4 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: BF1E7CEDD889900E

FASTA42644,756
        10         20         30         40         50         60 
MRILVIGSGG REHAILKGLA ADPATTDLHV APGSPAFASL ATVHADYKEV ADPARMLELA 

        70         80         90        100        110        120 
QDIKPELVVI GPEIPLVAGV ADTLRAEGIA VFGPNADAAQ IEGSKAFAKE VMEAAGVATA 

       130        140        150        160        170        180 
RAQTLPPGMT DDDIEHELDY FGPMYVVKDD GLAAGKGVVV TADRAEARQH IHLVHAAGNP 

       190        200        210        220        230        240 
VLLESFLDGP EVSLFCLVDG ETVVPLLPAQ DHKRAYDNDE GPNTGGMGAY TPLPWLSAEG 

       250        260        270        280        290        300 
VDRIVREVCE PVAKQMVERG TPYSGLLYAG LAWGQEGPSV IEFNCRFGDP ETQPLLSLLK 

       310        320        330        340        350        360 
TPLAGVLNAV ATGTLDELPA LEWEDAYAVT VVLAAANYPE SPRKGDAITS PDLADTDKIL 

       370        380        390        400        410        420 
HAGTAVKDAE VISNGGRVLN VIGKGETLSA ARAAAYEVLE NIELADSFYR TDIGQAAEEG 


RISIDS 

« Hide

References

[1]"Sequence analysis of Corynebacterium ammoniagenes purD/B/C region."
Yonetani Y., Teshiba S.
Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 6872 / DSM 20305 / KCTC 1019 / NCTC 2399.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB003161 Genomic DNA. Translation: BAA89446.1.

3D structure databases

ProteinModelPortalQ9RHX4.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ9RHX4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_CORAM
AccessionPrimary (citable) accession number: Q9RHX4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: May 1, 2000
Last modified: February 19, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways