Reviewed, UniProtKB/Swiss-Prot Q9RHX4 (PUR2_CORAM)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:

purD

Organism

Corynebacterium ammoniagenes (Brevibacterium ammoniagenes)

Taxonomic identifier

1697 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151445
Regions
	Domain	109 – 312	204	ATP-grasp
	Nucleotide binding	138 – 193	56	ATP By similarity
Sites
	Metal binding	282	1	Magnesium or manganese By similarity
	Metal binding	284	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9RHX4-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: BF1E7CEDD889900E	FASTA	426	44,756
	10 20 30 40 50 60 MRILVIGSGG REHAILKGLA ADPATTDLHV APGSPAFASL ATVHADYKEV ADPARMLELA 70 80 90 100 110 120 QDIKPELVVI GPEIPLVAGV ADTLRAEGIA VFGPNADAAQ IEGSKAFAKE VMEAAGVATA 130 140 150 160 170 180 RAQTLPPGMT DDDIEHELDY FGPMYVVKDD GLAAGKGVVV TADRAEARQH IHLVHAAGNP 190 200 210 220 230 240 VLLESFLDGP EVSLFCLVDG ETVVPLLPAQ DHKRAYDNDE GPNTGGMGAY TPLPWLSAEG 250 260 270 280 290 300 VDRIVREVCE PVAKQMVERG TPYSGLLYAG LAWGQEGPSV IEFNCRFGDP ETQPLLSLLK 310 320 330 340 350 360 TPLAGVLNAV ATGTLDELPA LEWEDAYAVT VVLAAANYPE SPRKGDAITS PDLADTDKIL 370 380 390 400 410 420 HAGTAVKDAE VISNGGRVLN VIGKGETLSA ARAAAYEVLE NIELADSFYR TDIGQAAEEG RISIDS

References

[1]	"Sequence analysis of Corynebacterium ammoniagenes purD/B/C region." Yonetani Y., Teshiba S. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6872 / DSM 20305 / KCTC 1019 / NCTC 2399.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB003161 Genomic DNA. Translation: BAA89446.1.
3D structure databases
HSSP	HSSP built from PDB template 1GSO based on UniProtKB P15640.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.3.4.13. 259964.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_CORAM

Accession

Primary (citable) accession number: Q9RHX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	May 1, 2000
Last modified:	September 22, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)