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Protein

Lysine--tRNA ligase

Gene

lysS

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi405Magnesium 1By similarity1
Metal bindingi412Magnesium 1By similarity1
Metal bindingi412Magnesium 2By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.6. 623.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligase (EC:6.1.1.6)
Alternative name(s):
Lysyl-tRNA synthetase
Short name:
LysRS
Gene namesi
Name:lysS
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001525991 – 494Lysine--tRNA ligaseAdd BLAST494

Proteomic databases

PRIDEiQ9RHV9.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

Secondary structure

1494
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 22Combined sources15
Helixi38 – 45Combined sources8
Helixi50 – 56Combined sources7
Beta strandi59 – 72Combined sources14
Beta strandi75 – 82Combined sources8
Beta strandi85 – 92Combined sources8
Helixi93 – 106Combined sources14
Beta strandi112 – 121Combined sources10
Beta strandi127 – 138Combined sources12
Helixi154 – 159Combined sources6
Helixi161 – 167Combined sources7
Helixi170 – 191Combined sources22
Beta strandi201 – 205Combined sources5
Beta strandi208 – 211Combined sources4
Beta strandi215 – 218Combined sources4
Turni219 – 222Combined sources4
Beta strandi223 – 227Combined sources5
Helixi232 – 240Combined sources9
Beta strandi245 – 253Combined sources9
Beta strandi264 – 274Combined sources11
Helixi278 – 297Combined sources20
Beta strandi300 – 304Combined sources5
Beta strandi307 – 310Combined sources4
Beta strandi316 – 319Combined sources4
Helixi320 – 327Combined sources8
Helixi338 – 347Combined sources10
Helixi358 – 369Combined sources12
Helixi371 – 373Combined sources3
Beta strandi378 – 381Combined sources4
Helixi385 – 387Combined sources3
Beta strandi400 – 408Combined sources9
Beta strandi411 – 419Combined sources9
Helixi423 – 438Combined sources16
Helixi449 – 456Combined sources8
Beta strandi461 – 468Combined sources8
Helixi469 – 476Combined sources8
Helixi482 – 484Combined sources3
Beta strandi486 – 488Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A74X-ray1.80A/B/C/D2-494[»]
3E9HX-ray2.10A/B/C/D2-494[»]
3E9IX-ray2.20A/B/C/D2-494[»]
ProteinModelPortaliQ9RHV9.
SMRiQ9RHV9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9RHV9.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

CDDicd00775. LysRS_core. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PIRSFiPIRSF039101. LysRS2. 1 hit.
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9RHV9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHEELNDQL RVRREKLKKI EELGVDPFGK RFERTHKAEE LFELYGDLSK
60 70 80 90 100
EELEEQQIEV AVAGRIMTKR GMGKAGFAHI QDVTGQIQIY VRQDDVGEQQ
110 120 130 140 150
YELFKISDLG DIVGVRGTMF KTKVGELSIK VSSYEFLTKA LRPLPEKYHG
160 170 180 190 200
LKDIEQRYRQ RYLDLIMNPE SKKTFITRSL IIQSMRRYLD SHGYLEVETP
210 220 230 240 250
MMHAVAGGAA ARPFITHHNA LDMTLYMRIA IELHLKRLIV GGLEKVYEIG
260 270 280 290 300
RVFRNEGIST RHNPEFTMLE LYEAYADFRD IMKLTENLIA HIATEVLGTT
310 320 330 340 350
KIQYGEHLVD LTPEWRRLHM VDAIKEYVGV DFWRQMSDEE ARELAKEHGV
360 370 380 390 400
EVAPHMTFGH IVNEFFEQKV EDKLIQPTFI YGHPVEISPL AKKNPDDPRF
410 420 430 440 450
TDRFELFIVG REHANAFTEL NDPIDQRQRF EEQLKEREQG NDEAHEMDED
460 470 480 490
FLEALEYGMP PTGGLGIGVD RLVMLLTNSP SIRDVLLFPQ MRHK
Length:494
Mass (Da):57,406
Last modified:May 1, 2000 - v1
Checksum:i109D1A4FDD7F714C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012100 Genomic DNA. Translation: BAA88691.1.
PIRiJC7205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012100 Genomic DNA. Translation: BAA88691.1.
PIRiJC7205.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A74X-ray1.80A/B/C/D2-494[»]
3E9HX-ray2.10A/B/C/D2-494[»]
3E9IX-ray2.20A/B/C/D2-494[»]
ProteinModelPortaliQ9RHV9.
SMRiQ9RHV9.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ9RHV9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi6.1.1.6. 623.

Miscellaneous databases

EvolutionaryTraceiQ9RHV9.

Family and domain databases

CDDicd00775. LysRS_core. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PIRSFiPIRSF039101. LysRS2. 1 hit.
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYK_GEOSE
AccessioniPrimary (citable) accession number: Q9RHV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.