Q9RH76 (DAPA_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	bll5072

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.
Sequence caution	The sequence BAC50337.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_0000103086
Regions
	Region	52 – 53	2	Pyruvate binding By similarity
Sites
	Active site	165	1	Schiff-base intermediate with substrate By similarity
	Binding site	110	1	Pyruvate By similarity
	Site	137	1	Involved in proton transfer during cleavage By similarity
Experimental info
	Sequence conflict	192	1	D → V in AAF04320. Ref.1
	Sequence conflict	201 – 202	2	HG → QC in AAF04320. Ref.1
	Sequence conflict	230 – 231	2	HA → QR in AAF04320. Ref.1
	Sequence conflict	243	1	L → V in AAF04320. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9RH76 [UniParc]. Last modified February 28, 2003. Version 2. Checksum: 69A6DAA6B7319E91	FASTA	296	32,098
	10 20 30 40 50 60 MAAKTKFRGS FTALVTPFKN GSLDEAAFRS LVNWQISEGT NGLVPVGTTG ESPTLSHDEH 70 80 90 100 110 120 KKVVEWCIEE AKGRVPVVAG AGSNSTKEAI ELAQHAEKAG ADAVLVVTPY YNKPTQEGMY 130 140 150 160 170 180 QHFKAINDAI GIPIIIYNIP PRSVIDMSVD TMKRLWELKN IAGVKDATAS MVRVSQQRAA 190 200 210 220 230 240 MGEDFNQLSG EDATILGYMA HGGHGCISVT SNVAPRLCSE FHTAWQKGDH ATALKLHDKL 250 260 270 280 290 MPLHNNLFIE SNPAPIKYAM SLLGKLDETL RLPMVPVTEP TRVAVRSAMV HAGLIN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Extended DNA sequencing in the upstream region of sipF in Bradyrhizobium japonicum." Mueller P., Stingel D. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: USDA 110spc4.
[2]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF065159 Genomic DNA. Translation: AAF04320.1. BA000040 Genomic DNA. Translation: BAC50337.1. Different initiation.
RefSeq	NP_771712.1. NC_004463.1.
3D structure databases
ProteinModelPortal	Q9RH76.
SMR	Q9RH76. Positions 7-295.
ModBase	Search...
Proteomic databases
PRIDE	Q9RH76.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1051802.
GenomeReviews	Gene locus bll5072 in contig BA000040_GR.
KEGG	bja:bll5072.
NMPDR	fig|224911.1.peg.5072.
PATRIC	21193814. VBIBraJap65052_5152.
Phylogenomic databases
HOGENOM	HBG358848.
OMA	HGCISVV.
PhylomeDB	Q9RH76.
ProtClustDB	PRK03170.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL5072-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR020624. Dihydrodipicolinate_synth_CS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_BRAJA

Accession

Primary (citable) accession number: Q9RH76

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	February 28, 2003
Last modified:	January 25, 2012
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program