Beta-agarase A precursor - Zobellia galactanivorans (strain DSM 12802 / CIP 106680 / NCIMB 13871 / Dsij)

Preferred order of sections

Names and origin

Protein names

Submitted name:
Beta-agarase A EMBL AAF21820.1
EC=3.2.1.81 EMBL AAF21820.1

Gene names

Name:

agaA EMBL AAF21820.1

Organism

Zobellia galactanivorans (strain DSM 12802 / CIP 106680 / NCIMB 13871 / Dsij) EMBL AAF21820.1

Taxonomic identifier

63186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Zobellia

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

Ontologies

Keywords
Domain	Signal EMBL AAF21820.1
Ligand	Calcium PDB 1O4Y PDB 1URX Metal-binding PDB 1O4Y PDB 1URX
Molecular function	Glycosidase EMBL AAF21820.1 Hydrolase
Technical term	3D-structure PDB 1O4Y PDB 1URX
Gene Ontology (GO)
Molecular function	beta-agarase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Potential EMBL AAF21820.1

Chain

20 – 539

520

Potential EMBL AAF21820.1

PRO_5000055268

Chain

20 – 295

276

beta-agarase A EMBL AAF21820.1

PRO_5000055269

Regions

Region

82 – 92

11

Galactose binding PDB 1URX

Region

96 – 98

3

Galactose binding PDB 1URX

Sites

Metal binding

22

1

Calcium PDB 1O4Y PDB 1URX

Metal binding

47

1

Calcium; via carbonyl oxygen PDB 1O4Y PDB 1URX

Metal binding

49

1

Calcium PDB 1O4Y PDB 1URX

Metal binding

91

1

Calcium; via carbonyl oxygen PDB 1O4Y PDB 1URX

Metal binding

279

1

Calcium PDB 1O4Y PDB 1URX

Binding site

73

1

Galactose PDB 1URX

Binding site

144

1

Galactose PDB 1URX

Binding site

176

1

Galactose PDB 1URX

Binding site

271

1

Galactose PDB 1URX

Sequences

Sequence

Length

Mass (Da)

Tools

Q9RGX9 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 611AA1B1C509563A
Show »

FASTA

539

59,994

        10         20         30         40         50         60 
MKKNYLLLYF IFLLCGSIAA QDWNGIPVPA NPGNGMTWQL QDNVSDSFNY TSSEGNRPTA 

        70         80         90        100        110        120 
FTSKWKPSYI NGWTGPGSTI FNAAQAWTNG SQLAIQAQPA GNGKSYNGII TSKNKIQYPV 

       130        140        150        160        170        180 
YMEIKAKIMD QVLANAFWTL TDDETQEIDI MEGYGSDRGG TWFAQRMHLS HHTFIRNPFT 

       190        200        210        220        230        240 
DYQPMGDATW YYNGGTPWRS AYHRYGCYWK DPFTLEYYID GVKVRTVTRA EIDPNNHLGG 

       250        260        270        280        290        300 
TGLNQATNII IDCENQTDWR PAATQEELAD DSKNIFWVDW IRVYKPVAVS GGGNNGNDGA 

       310        320        330        340        350        360 
TEFQYDLGTD TSAVWPGYTR VSNTTRAGNF GWANTNDIGS RDRGASNGRN NINRDINFSS 

       370        380        390        400        410        420 
QTRFFTQDLS NGTYNVLITF GDTYARKNMN VAAEGQNKLT NINTNAGQYV SRSFDVNVND 

       430        440        450        460        470        480 
GKLDLRFSVG NGGDVWSITR IWIRKVTSNS ANLLAAKGLT LEDPVETTEF LYPNPAKTDD 

       490        500        510        520        530 
FVTVPNSEIG SSIIIYNSAG QVVKKVSVVS ENQKISLEGF AKGMYFINLN GQSTKLIVQ

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References

[1]	"The endo-beta-agarases AgaA and AgaB from the marine bacterium Zobellia galactanivorans: two paralogue enzymes with different molecular organizations and catalytic behaviours." Jam M., Flament D., Allouch J., Potin P., Thion L., Kloareg B., Czjzek M., Helbert W., Michel G., Barbeyron T. Biochem. J. 385:703-713(2005) [PubMed: 15456406] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Dsij EMBL AAF21820.1.
[2]	"The three-dimensional structures of two beta-agarases." Allouch J., Jam M., Helbert W., Barbeyron T., Kloareg B., Henrissat B., Czjzek M. J. Biol. Chem. 278:47171-47180(2003) [PubMed: 12970344] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.48 ANGSTROMS) OF 20-295 IN COMPLEX WITH CALCIUM.
[3]	"Parallel substrate binding sites in a beta-agarase suggest a novel mode of action on double-helical agarose." Allouch J., Helbert W., Henrissat B., Czjzek M. Structure 12:623-632(2004) [PubMed: 15062085] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 20-290 IN COMPLEX WITH CALCIUM AND GALACTOSE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF098954 Genomic DNA. Translation: AAF21820.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1O4Y	X-ray	1.48	A	20-295	[»]
1URX	X-ray	1.70	A	20-290	[»]

ProteinModelPortal

Q9RGX9.

SMR

Q9RGX9. Positions 20-289.

ModBase

Search...

Protein family/group databases

CAZy

GH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR008985. ConA-like_lec_gl.
IPR013320. ConA-like_subgrp.
[Graphical view]

Gene3D

G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.

ProtoNet

Search...

Entry information

Entry name

Q9RGX9_ZOBGA

Accession

Primary (citable) accession number: Q9RGX9

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 2000
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)