Na(+)-translocating NADH-quinone reductase subunit F - Vibrio harveyi (strain ATCC BAA-1116 / BB120)

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Q9RFV6 (NQRF_VIBHB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 90. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Na(+)-translocating NADH-quinone reductase subunit F
Short name=Na(+)-NQR subunit F
Short name=Na(+)-translocating NQR subunit F
EC=1.6.5.-

Alternative name(s):
NQR complex subunit F
NQR-1 subunit F

Gene names

Name:	nqrF
Ordered Locus Names:	VIBHAR_03270

Organism

Vibrio harveyi (strain ATCC BAA-1116 / BB120) [Complete proteome] [HAMAP]

Taxonomic identifier

338187 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio ›

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na⁺ ions from the cytoplasm to the periplasm. The first step is catalyzed by NqrF, which accepts electrons from NADH and reduces ubiquinone-1 to ubisemiquinone by a one-electron transfer pathway. HAMAP-Rule MF_00430
Catalytic activity	NADH + ubiquinone + Na⁺(In) = NAD⁺ + ubiquinol + Na⁺(Out). HAMAP-Rule MF_00430
Cofactor	Binds 1 2Fe-2S cluster By similarity. FAD By similarity.
Subunit structure	Composed of six subunits; NqrA, NqrB, NqrC, NqrD, NqrE and NqrF By similarity.
Subcellular location	Cell inner membrane Potential HAMAP-Rule MF_00430.
Sequence similarities	Belongs to the NqrF family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Ion transport Sodium transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	2Fe-2S FAD Flavoprotein Iron Iron-sulfur Metal-binding NAD Sodium Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	sodium ion transport Inferred from electronic annotation. Source: HAMAP
Cellular_component	Gram-negative-bacterium-type cell wall Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 407

407

Na(+)-translocating NADH-quinone reductase subunit F HAMAP-Rule MF_00430

PRO_0000074507

Regions

Transmembrane

5 – 24

Helical; Potential

Domain

32 – 126

2Fe-2S ferredoxin-type

Domain

129 – 269

141

FAD-binding FR-type

Region

272 – 389

118

Catalytic HAMAP-Rule MF_00430

Sites

Metal binding

Iron-sulfur (2Fe-2S) By similarity

Metal binding

Iron-sulfur (2Fe-2S) By similarity

Metal binding

Iron-sulfur (2Fe-2S) By similarity

Metal binding

110

Iron-sulfur (2Fe-2S) By similarity

Experimental info

Sequence conflict

80 – 82

VKV → RKI in AAF15416. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q9RFV6 [UniParc].

Last modified November 13, 2007. Version 2.
Checksum: F57A122254FBFF84
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FASTA

407

45,087

        10         20         30         40         50         60 
MDIILGVVMF TLIVLALVLV ILFAKSKLVP TGDITISVND DPSLAIVTQP GGKLLSALAG 

        70         80         90        100        110        120 
AGVFVSSACG GGGSCGQCRV KVKSGGGDIL PTELDHITKG EAREGERLAC QVAMKTDMDI 

       130        140        150        160        170        180 
ELPEEIFGVK KWECTVISND NKATFIKELK LQIPDGESVP FRAGGYIQIE APAHHVKYAD 

       190        200        210        220        230        240 
YDIPEEYRED WEKFNLFRYE SKVNEETIRA YSMANYPEEH GIIMLNVRIA TPPPNNPDVA 

       250        260        270        280        290        300 
PGIMSSFIWS LKEGDKCTIS GPFGEFFAKD TDAEMVFVGG GAGMAPMRSH IFDQLKRLHS 

       310        320        330        340        350        360 
KRKMSFWYGA RSKREMFYVE DFDGLAADND NFVWHCALSD PLPEDNWDGY TGFIHNVLYE 

       370        380        390        400 
NYLRDHEAPE DCEYYMCGPP MMNAAVIGML KDLGVEDENI LLDDFGG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and preliminary characterization of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio harveyi." Zhou W., Bertsova Y.V., Feng B., Tsatsos P., Verkhovskaya M.L., Gennis R.B., Bogachev A.V., Barquera B. Biochemistry 38:16246-16252(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	The Vibrio harveyi Genome Sequencing Project Bassler B., Clifton S.W., Fulton L., Delehaunty K., Fronick C., Harrison M., Markivic C., Fulton R., Tin-Wollam A.-M., Shah N., Pepin K., Nash W., Thiruvilangam P., Bhonagiri V., Waters C., Tu K.C., Irgon J., Wilson R.K. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1116 / BB120.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF165980 Genomic DNA. Translation: AAF15416.1. CP000789 Genomic DNA. Translation: ABU72218.1.
RefSeq	YP_001446445.1. NC_009783.1.
3D structure databases
ProteinModelPortal	Q9RFV6.
SMR	Q9RFV6. Positions 128-406.
ModBase	Search...
Protein-protein interaction databases
STRING	338187.VIBHAR_03270.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABU72218; ABU72218; VIBHAR_03270.
GeneID	5554705.
KEGG	vha:VIBHAR_03270.
PATRIC	20133171. VBIVibHar24526_3115.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2871.
HOGENOM	HOG000263661.
KO	K00351.
OMA	ANYPEEK.
ProtClustDB	PRK05464.
Family and domain databases
Gene3D	3.10.20.30. 1 hit.
HAMAP	MF_00430. NqrF.
InterPro	IPR001041. 2Fe-2S_ferredoxin-type. IPR012675. Beta-grasp_dom. IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR010205. NADH_Q_Rdtase_suF. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00111. Fer2. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00371. FPNCR.
SUPFAM	SSF54292. Ferredoxin. 1 hit. SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
TIGRFAMs	TIGR01941. nqrF. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NQRF_VIBHB

Accession

Primary (citable) accession number: Q9RFV6
Secondary accession number(s): A7N1U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents