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Q9RFN0

- BGAL_CARML

UniProt

Q9RFN0 - BGAL_CARML

Protein

Beta-galactosidase BgaB

Gene

bgaB

Organism
Carnobacterium maltaromaticum (Carnobacterium piscicola)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Capable of hydrolyzing the chromogen 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) at low temperatures. pNP-beta-galactoside, pNP-beta-fucoside and pNP-beta-galacturonide are also hydrolyzed, but not pNP-beta-galactosidase, pNP-alpha-galactoside, pNP-beta-mannoside, pNP-beta-arabanoside, pNP-beta-xyloside, pNP-beta-glucuronide nor pNP-betacellobioside at 20 degrees Celsius.1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

    Kineticsi

    1. KM=1.7 mM for o-nitrophenyl-beta-D-galactopyranoside (ONPG) (at 30 degrees Celsius)1 Publication
    2. KM=1.04 mM for ONPG (at 25 degrees Celsius)1 Publication

    Vmax=450 µmol/min/mg enzyme with ONPG as substrate (at 30 degrees Celsius)1 Publication

    Temperature dependencei

    Optimum temperature is 30 degrees Celsius for activity with ONPG as substrate. Loses half of its activity at 30 degrees Celsius within 1 hour. Inactivated at 40 degrees Celsius in 10 minutes. In the absence of glycerol, the enzyme is extremely unstable even at low-temperature incubation, with an 85% loss of activity at 20 degrees Celsius after 1 hour.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei109 – 1091SubstrateBy similarity
    Metal bindingi113 – 1131ZincBy similarity
    Binding sitei147 – 1471SubstrateBy similarity
    Active sitei148 – 1481Proton donorBy similarity
    Metal bindingi156 – 1561ZincBy similarity
    Metal bindingi158 – 1581ZincBy similarity
    Metal bindingi161 – 1611ZincBy similarity
    Active sitei302 – 3021NucleophileBy similarity
    Binding sitei310 – 3101SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. galactose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    CAZyiGH42. Glycoside Hydrolase Family 42.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase BgaB (EC:3.2.1.23)
    Short name:
    Beta-galBy similarity
    Gene namesi
    Name:bgaBImported
    OrganismiCarnobacterium maltaromaticum (Carnobacterium piscicola)
    Taxonomic identifieri2751 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesCarnobacteriaceaeCarnobacterium

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 668668Beta-galactosidase BgaBPRO_0000407686Add
    BLAST

    Proteomic databases

    PRIDEiQ9RFN0.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9RFN0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni350 – 3534Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9RFN0-1 [UniParc]FASTAAdd to Basket

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    MLQQKKLFYG GDYNPEQWSK AIILEDMRLM KKANVNYVSL NIFGWASIQP    50
    TEEGFDFSFL DEMLDLLWEN GIGIDLANGT ASPPAWLVKK HPEILPVTSQ 100
    GTPLVHGSRQ HYCPSNKVYR SYVIRLTEEV AKRYATHPGI VMWHVNNEYT 150
    CHISECYCES CEKSFRQWLQ MKYKKINTLN ECWSTKFWSQ SYSQWDEIFL 200
    PKEMPTFKNP AHQLDYKRFI SDQNLTLFKA EKKAIRSYSK DIPVMTNLMG 250
    LHKHVDGFAF AEEMDVVGWD SYPNPFEEKP YPQFLANDLT RSLKKKPFLV 300
    MEQAPSAVNW RRANGAKSPG QMRLWSYEAL AHGADGILFF QWRQSQGGAE 350
    KFHSGMVSHN QDTNSRIFKE VVQLGTEMSQ LDELVGTNYN AEVAIVFDWE 400
    NWWALELDAK PSGEINYIKQ MRDLYTIFHE LNIGVDFIHP KEDLSNYKLV 450
    LSIAQYLVTD DFSAKVKRYI KAGGHFLTTF FSGIVDEYDR VYLGGYPGAF 500
    KEVLGIYVEE FDPMPIGRKS QIKYGETYYT TELWKEVIHL QGAETIATFT 550
    EGYLMGQPAL TKFGYGKGKT YYMGTKLAKD GNMKFIQTIL AESKIQPLNQ 600
    VEIESENSKI SMTCRSNSSH DYIFLLNYGQ TSEKVKLKKG GQSLLDGSMV 650
    EGEVSVKAND VKIIKLTK 668
    Length:668
    Mass (Da):76,789
    Last modified:May 1, 2000 - v1
    Checksum:i6B256F81C06C4F17
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF184246 Genomic DNA. Translation: AAF16519.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF184246 Genomic DNA. Translation: AAF16519.1 .

    3D structure databases

    ProteinModelPortali Q9RFN0.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH42. Glycoside Hydrolase Family 42.

    Proteomic databases

    PRIDEi Q9RFN0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProi IPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001084. B-galactosidase. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Biochemical and phylogenetic analyses of a cold-active beta-galactosidase from the lactic acid bacterium Carnobacterium piscicola BA."
      Coombs J.M., Brenchley J.E.
      Appl. Environ. Microbiol. 65:5443-5450(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.
      Strain: BAImported.

    Entry informationi

    Entry nameiBGAL_CARML
    AccessioniPrimary (citable) accession number: Q9RFN0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3