Q9RFD6 (BCHL_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein
Short name=DPOR subunit L
Short name=LI-POR subunit L
EC=1.18.-.-

Gene names

Name:	bchL
Ordered Locus Names:	RHOS4_18930
ORF Names:	RSP_0288

Organism

Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) [Reference proteome] [HAMAP]

Taxonomic identifier

272943 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter › Rhodobacter sphaeroides

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). This reaction is light-independent By similarity. HAMAP-Rule MF_00355
Cofactor	Binds 1 4Fe-4S cluster per dimer By similarity.
Pathway	Porphyrin biosynthesis; bacteriochlorophyll biosynthesis (light-independent). HAMAP-Rule MF_00355
Subunit structure	Protochlorophyllide reductase is thought to be composed of three subunits; BchL, BchN and BchB. Homodimer of BchL subunit By similarity.
Sequence similarities	Belongs to the NifH/BchL/ChlL family.

Ontologies

Keywords
Biological process	Bacteriochlorophyll biosynthesis Chlorophyll biosynthesis Photosynthesis
Ligand	4Fe-4S ATP-binding Iron Iron-sulfur Metal-binding Nucleotide-binding
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	light-independent bacteriochlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway photosynthesis, dark reaction Inferred from electronic annotation. Source: InterPro
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on iron-sulfur proteins as donors Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 297

297

Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein HAMAP-Rule MF_00355

PRO_0000139582

Regions

Nucleotide binding

38 – 45

8

ATP Potential

Sites

Metal binding

126

1

Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity

Metal binding

160

1

Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity

Experimental info

Sequence conflict

14

1

G → V in AAF24272. Ref.1

Sequence conflict

194

1

V → A in CAB38722. Ref.2

Sequence conflict

261 – 262

2

AA → VV in AAF24272. Ref.1

Sequence conflict

269

1

L → V in CAB38722. Ref.2

Sequence conflict

273

1

L → F in AAF24272. Ref.1

Sequence conflict

278

1

D → Y in AAF24272. Ref.1

Sequence conflict

284

1

S → R in AAF24272. Ref.1

Sequence conflict

289

1

D → E in AAF24272. Ref.1

Secondary structure

1

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297

Helix Strand Turn

Details...

Beta strand

32 – 37

6

Helix

44 – 57

14

Beta strand

62 – 70

9

Helix

75 – 78

4

Helix

85 – 91

7

Helix

96 – 98

3

Helix

101 – 104

4

Helix

109 – 111

3

Beta strand

113 – 116

4

Helix

129 – 140

12

Turn

141 – 144

4

Beta strand

148 – 154

7

Helix

161 – 168

8

Beta strand

171 – 177

7

Helix

181 – 195

15

Turn

196 – 200

5

Beta strand

204 – 212

9

Helix

217 – 226

10

Beta strand

230 – 234

5

Helix

238 – 245

8

Turn

250 – 252

3

Helix

257 – 275

19

Helix

287 – 293

7

Sequences

	Sequence		Length	Mass (Da)
	Q9RFD6 [UniParc]. Last modified January 24, 2006. Version 2. Checksum: BDDE6F40A21952CE	FASTA	297	32,295
	10 20 30 40 50 60 MSPKDLTIPT GADGEGSVQV HLDEADKITG AKVFAVYGKG GIGKSTTSSN LSAAFSILGK 70 80 90 100 110 120 RVLQIGCDPK HDSTFTLTGS LVPTVIDVLK DVDFHPEELR PEDFVFEGFN GVMCVEAGGP 130 140 150 160 170 180 PAGTGCGGYV VGQTVKLLKQ HHLLDDTDVV IFDVLGDVVC GGFAAPLQHA DQAVVVTAND 190 200 210 220 230 240 FDSIYAMNRI IAAVQAKSKN YKVRLAGCVA NRSRATDEVD RFCKETNFRR LAHMPDLDAI 250 260 270 280 290 RRSRLKKKTL FEMDEDQDVL AARAEYIRLA ESLWRGLDPI DPHSLPDRDI FELLGFD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence analysis of the photosynthesis region of Rhodobacter sphaeroides 2.4.1." Choudhary M., Kaplan S. Nucleic Acids Res. 28:862-867(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The photosynthesis gene cluster of Rhodobacter sphaeroides." Naylor G.W., Addlesee H.A., Gibson L.C.D., Hunter C.N. Photosyn. Res. 62:121-139(1999) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF195122 Genomic DNA. Translation: AAF24272.1.
AJ010302 Genomic DNA. Translation: CAB38722.1.
CP000143 Genomic DNA. Translation: ABA79461.1.

PIR

T50728.

RefSeq

YP_353362.1. NC_007493.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3FWY	X-ray	1.63	A/B	2-297	[»]

ProteinModelPortal

Q9RFD6.

ModBase

Search...

Protein-protein interaction databases

STRING

272943.RSP_0288.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3719200.

KEGG

rsp:RSP_0288.

PATRIC

23153714. VBIRhoSph57909_2232.

Phylogenomic databases

eggNOG

COG1348.

HOGENOM

HOG000228825.

KO

K04037.

OMA

TSCNISV.

ProtClustDB

PRK13185.

Enzyme and pathway databases

BioCyc

RSPH272943:GJAS-1945-MONOMER.

UniPathway

UPA00671.

Family and domain databases

HAMAP

MF_00355. ChlL_BchL.

InterPro

IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR005971. Protochlorophyllide_ATP-bd.
[Graphical view]

Pfam

PF00142. Fer4_NifH. 1 hit.
[Graphical view]

PIRSF

PIRSF000363. Nitrogenase_iron. 1 hit.

PRINTS

PR00091. NITROGNASEII.

TIGRFAMs

TIGR01281. DPOR_bchL. 1 hit.

PROSITE

PS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9RFD6.

Entry information

Entry name

BCHL_RHOS4

Accession

Primary (citable) accession number: Q9RFD6
Secondary accession number(s): Q3J173, Q9Z5E1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	January 24, 2006
Last modified:	May 1, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program