Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

6,7-dimethyl-8-ribityllumazine synthase

Gene

ribH

Organism
Bartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1) (Rochalimaea henselae)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.UniRule annotation

Catalytic activityi

1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H2O + phosphate.UniRule annotation

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 6,7-dimethyl-8-ribityllumazine synthase (ribH)
  2. no protein annotated in this organism
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei185-amino-6-(D-ribitylamino)uracilUniRule annotation1
Active sitei83Proton donorUniRule annotation1
Binding sitei1085-amino-6-(D-ribitylamino)uracil; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei1221-deoxy-L-glycero-tetrulose 4-phosphateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processRiboflavin biosynthesis

Enzyme and pathway databases

BRENDAi2.5.1.78 7854
UniPathwayiUPA00275; UER00404

Names & Taxonomyi

Protein namesi
Recommended name:
6,7-dimethyl-8-ribityllumazine synthaseUniRule annotation (EC:2.5.1.78UniRule annotation)
Short name:
DMRL synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lumazine synthaseUniRule annotation
Gene namesi
Name:ribHUniRule annotation
Synonyms:ribE
Ordered Locus Names:BH07580
OrganismiBartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1) (Rochalimaea henselae)
Taxonomic identifieri283166 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBartonellaceaeBartonella
Proteomesi
  • UP000000421 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001347171 – 1556,7-dimethyl-8-ribityllumazine synthaseAdd BLAST155

Proteomic databases

PaxDbiQ9REF4
PRIDEiQ9REF4

Interactioni

Protein-protein interaction databases

STRINGi283166.BH07580

Structurei

3D structure databases

ProteinModelPortaliQ9REF4
SMRiQ9REF4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 515-amino-6-(D-ribitylamino)uracil bindingUniRule annotation3
Regioni75 – 775-amino-6-(D-ribitylamino)uracil bindingUniRule annotation3
Regioni80 – 811-deoxy-L-glycero-tetrulose 4-phosphate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the DMRL synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UTT Bacteria
COG0054 LUCA
HOGENOMiHOG000229250
KOiK00794
OMAiHGNKGTE
OrthoDBiPOG091H021R

Family and domain databases

CDDicd09209 Lumazine_synthase-I, 1 hit
Gene3Di3.40.50.960, 1 hit
HAMAPiMF_00178 Lumazine_synth, 1 hit
InterProiView protein in InterPro
IPR034964 LS
IPR002180 LS/RS
IPR036467 LS/RS_sf
PANTHERiPTHR21058 PTHR21058, 1 hit
PfamiView protein in Pfam
PF00885 DMRL_synthase, 1 hit
SUPFAMiSSF52121 SSF52121, 1 hit
TIGRFAMsiTIGR00114 lumazine-synth, 1 hit

Sequencei

Sequence statusi: Complete.

Q9REF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIEICKKLH VLIVEARFYD GISDALLTGA VSTLQKAEAT YDIVTVPGAL
60 70 80 90 100
EIPGAIAFAE KNSKIYYDGY VALGCVIRGE TYHFEIVAND SCRALMDLTI
110 120 130 140 150
HKHLAIGNGI LTVENEKQAW ARAKQDEKNK GGFAAQAALC MIALKKRFGE

IIKYG
Length:155
Mass (Da):16,953
Last modified:August 31, 2004 - v2
Checksum:i26590561428BC2AE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95L → S in CAB63092 (PubMed:10488170).Curated1
Sequence conflicti126D → A in CAB63092 (PubMed:10488170).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132928 Genomic DNA Translation: CAB63092.1
BX897699 Genomic DNA Translation: CAF27559.1
RefSeqiWP_011180660.1, NZ_LRIJ01000002.1

Genome annotation databases

EnsemblBacteriaiCAF27559; CAF27559; BH07580
GeneIDi29620737
KEGGibhe:BH07580

Similar proteinsi

Entry informationi

Entry nameiRISB_BARHE
AccessioniPrimary (citable) accession number: Q9REF4
Secondary accession number(s): Q6G3K9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: August 31, 2004
Last modified: March 28, 2018
This is version 95 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health