Q9REF4 (RISB_BARHE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6,7-dimethyl-8-ribityllumazine synthase
Short name=DMRL synthase
Short name=Lumazine synthase
EC=2.5.1.9

Alternative name(s):
Riboflavin synthase beta chain

Gene names

Name:	ribH
Synonyms:	ribE
Ordered Locus Names:	BH07580

Organism

Bartonella henselae (strain ATCC 49882 / Houston 1) (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

283166 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 155	155	6,7-dimethyl-8-ribityllumazine synthase HAMAP MF_00178		PRO_0000134717
Experimental info
	Sequence conflict	95	1	L → S in CAB63092. Ref.1
	Sequence conflict	126	1	D → A in CAB63092. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9REF4 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: 26590561428BC2AE	FASTA	155	16,953
	10 20 30 40 50 60 MTIEICKKLH VLIVEARFYD GISDALLTGA VSTLQKAEAT YDIVTVPGAL EIPGAIAFAE 70 80 90 100 110 120 KNSKIYYDGY VALGCVIRGE TYHFEIVAND SCRALMDLTI HKHLAIGNGI LTVENEKQAW 130 140 150 ARAKQDEKNK GGFAAQAALC MIALKKRFGE IIKYG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analysis of riboflavin synthesis genes in Bartonella henselae and use of the ribC gene for differentiation of Bartonella species by PCR." Bereswill S., Hinkelmann S., Kist M., Sander A. J. Clin. Microbiol. 37:3159-3166(1999) [PubMed: 10488170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.
[2]	"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ132928 Genomic DNA. Translation: CAB63092.1. BX897699 Genomic DNA. Translation: CAF27559.1.
RefSeq	YP_033570.1. NC_005956.1.
3D structure databases
ProteinModelPortal	Q9REF4.
SMR	Q9REF4. Positions 10-149.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2864803.
GenomeReviews	Gene locus BH07580 in contig BX897699_GR.
KEGG	bhe:BH07580.
NMPDR	fig|283166.1.peg.698.
PATRIC	20545332. VBIBarHen29080_0811.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG311126.
OMA	SSRALMD.
PhylomeDB	Q9REF4.
ProtClustDB	PRK00061.
Enzyme and pathway databases
BioCyc	BHEN283166:BH07580-MONOMER.
Family and domain databases
HAMAP	MF_00178. Lumazine_synth. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
KO	K00794.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
SUPFAM	SSF52121. DMRL_synthase. 1 hit.
TIGRFAMs	TIGR00114. Lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB_BARHE

Accession

Primary (citable) accession number: Q9REF4
Secondary accession number(s): Q6G3K9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	August 31, 2004
Last modified:	January 25, 2012
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program