Endo-1,4-beta-xylanase - Bacillus sp. 41M-1

Preferred order of sections

Names and origin

Protein names

Recommended name:
Endo-1,4-beta-xylanase RuleBase RU004392
EC=3.2.1.8 RuleBase RU004392

Gene names

Name:

xynJ EMBL BAA82316.1

Organism

Bacillus sp. 41M-1 EMBL BAA82316.1

Taxonomic identifier

98930 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans. RuleBase RU004392
Pathway	Glycan degradation; xylan degradation. RuleBase RU004392
Sequence similarities	Belongs to the glycosyl hydrolase 11 (cellulase G) family. RuleBase RU003433

Ontologies

Keywords
Biological process	Xylan degradation RuleBase RU003433 EMBL BAA82316.1
Ligand	Calcium PDB 2DCK PDB 2DCJ Metal-binding PDB 2DCK PDB 2DCJ
Molecular function	Glycosidase RuleBase RU003433 Hydrolase
Technical term	3D-structure PDB 2DCK PDB 2DCJ
Gene Ontology (GO)
Biological process	xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro endo-1,4-beta-xylanase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

28 – 354

327

xylanase J EMBL BAA82316.1

PRO_5000049413

Sites

Metal binding

240

1

Calcium 1 PDB 2DCK PDB 2DCJ

Metal binding

242

1

Calcium 1 PDB 2DCK PDB 2DCJ

Metal binding

259

1

Calcium 1; via carbonyl oxygen PDB 2DCK PDB 2DCJ

Metal binding

264

1

Calcium 2; via carbonyl oxygen PDB 2DCK PDB 2DCJ

Metal binding

340

1

Calcium 2 PDB 2DCK PDB 2DCJ

Metal binding

344

1

Calcium 2; via carbonyl oxygen PDB 2DCK PDB 2DCJ

Metal binding

345

1

Calcium 2 PDB 2DCK PDB 2DCJ

Metal binding

349

1

Calcium 1 PDB 2DCK PDB 2DCJ

Sequences

Sequence

Length

Mass (Da)

Tools

Q9RC94 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 5F81EBB473151DC8
Show »

FASTA

354

39,000

        10         20         30         40         50         60 
MKQVKIMFLM TMFLGIGLLF FSENAEAAIT SNEIGTHDGY DYEFWKDSGG SGSMTLNSGG 

        70         80         90        100        110        120 
TFSAQWSNVN NILFRKGKKF DETQTHQQIG NMSINYGATY NPNGNSYLTV YGWTVDPLVE 

       130        140        150        160        170        180 
FYIVDSWGTW RPPGGTPKGT INVDGGTYQI YETTRYNQPS IKGTATFQQY WSVRTSKRTS 

       190        200        210        220        230        240 
GTISVSEHFR AWESLGMNMG NMYEVALTVE GYQSSGSANV YSNTLTIGGQ SGGEQATRVE 

       250        260        270        280        290        300 
AESMTKGGPY TSNITSPFNG VALYANGDNV SFNHSFTKAN SSFSLRGASN NSNMARVDLR 

       310        320        330        340        350 
IGGQNRGTFY FGDQYPAVYT INNINHGIGN QLVELIVTAD DGTWDAYLDY LEIR

« Hide

References

[1]	"Nucleotide sequence and mutational analysis of the gene encoding the novel alkaline xylanase from alkaliphilic Bacillus sp. strain 41M-1." Nakai R., Wakabayashi K., Asano T., Aono R., Horikoshi K., Nakamura S. Nucleic Acids Symp. Ser. 31:235-236(1994) Cited for: NUCLEOTIDE SEQUENCE. Strain: 41M-1 EMBL BAA82316.1.
[2]	Nakamura S., Nakai R., Wakabayashi K. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 41M-1 EMBL BAA82316.1.
[3]	"A two-domain structure of alkaliphilic XynJ from Bacillus sp. 41M-1." Ihsanawati, Tanaka N., Nakamura S., Kumasaka T. Submitted (JAN-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH CALCIUM.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB029319 Genomic DNA. Translation: BAA82316.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DCJ	X-ray	2.24	A/B	1-354	[»]
2DCK	X-ray	2.10	A	1-354	[»]

ProteinModelPortal

Q9RC94.

SMR

Q9RC94. Positions 28-353.

ModBase

Search...

Protein family/group databases

CAZy

CBM36. Carbohydrate-Binding Module Family 36.
GH11. Glycoside Hydrolase Family 11.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR005084. CMB_fam6.
IPR008985. ConA-like_lec_gl.
IPR008979. Galactose-bd-like.
IPR001137. Glyco_hydro_11.
IPR013319. Glyco_hydro_11/12_cat.
IPR018208. Glyco_hydro_11_AS.
[Graphical view]

Gene3D

G3DSA:2.60.120.180. Glyco_hydro_11/12_cat. 1 hit.

Pfam

PF03422. CBM_6. 1 hit.
PF00457. Glyco_hydro_11. 1 hit.
[Graphical view]

PRINTS

PR00911. GLHYDRLASE11.

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.
SSF49785. Gal_bind_like. 1 hit.

PROSITE

PS51175. CBM6. 1 hit.
PS00776. GLYCOSYL_HYDROL_F11_1. 1 hit.
PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q9RC94_9BACI

Accession

Primary (citable) accession number: Q9RC94

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 2000
Last sequence update:	May 1, 2000
Last modified:	June 28, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)