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Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene

pdxA

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).UniRule annotation

Catalytic activityi

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

Cofactori

a divalent metal cationUniRule annotationNote: Binds 1 divalent metal cation per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei140 – 1401SubstrateUniRule annotation
Binding sitei141 – 1411SubstrateUniRule annotation
Metal bindingi170 – 1701Divalent metal cation; shared with dimeric partnerUniRule annotation
Metal bindingi214 – 2141Divalent metal cation; shared with dimeric partnerUniRule annotation
Metal bindingi269 – 2691Divalent metal cation; shared with dimeric partnerUniRule annotation
Binding sitei277 – 2771SubstrateUniRule annotation
Binding sitei295 – 2951SubstrateUniRule annotation

GO - Molecular functioni

  1. 4-hydroxythreonine-4-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD binding Source: InterPro

GO - Biological processi

  1. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
  2. pyridoxine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyridoxine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, NADP

Enzyme and pathway databases

BioCyciBHAL272558:GJC5-862-MONOMER.
UniPathwayiUPA00244; UER00312.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxythreonine-4-phosphate dehydrogenaseUniRule annotation (EC:1.1.1.262UniRule annotation)
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenaseUniRule annotation
Gene namesi
Name:pdxAUniRule annotation
Ordered Locus Names:BH0804
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001258: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3343344-hydroxythreonine-4-phosphate dehydrogenasePRO_0000188797Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272558.BH0804.

Structurei

3D structure databases

ProteinModelPortaliQ9RC88.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PdxA family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1995.
HOGENOMiHOG000040097.
KOiK00097.
OMAiTHGDING.
OrthoDBiEOG6GN6ZC.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_00536. PdxA.
InterProiIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamiPF04166. PdxA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00557. pdxA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9RC88-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKKPIIAIP MGDPAGIGPE ITVGALNKKE LYDVANPVVI GHGDMLEKML
60 70 80 90 100
PVMKADLTIN RITTVDEAMF EYGTIDVIHL DNLNVAEVKM GTVQAQCGKA
110 120 130 140 150
AFEYIRHAVQ LANDKKVDAL ATTPINKESL KAAEVPYIGH TEMLADLTKT
160 170 180 190 200
EDPLTMFEVH SMRIFFLTRH LSLKDAIDQM TKERVHDYLL RCDKALEKLG
210 220 230 240 250
VKERRFAVAG LNPHSGENGL FGREEMDEIT PGIELAKKDG INAVGPVPAD
260 270 280 290 300
SVFHHALNGR YDAVLSLYHD QGHIAAKMTD FERTISITNG LPFLRTSVDH
310 320 330
GTAFDIAGKG IASTVSMEEC IKLAAKYAPH FVTA
Length:334
Mass (Da):36,632
Last modified:May 1, 2000 - v1
Checksum:i24436A1866079820
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB024550 Genomic DNA. Translation: BAA83911.1.
BA000004 Genomic DNA. Translation: BAB04523.1.
PIRiD83750.
RefSeqiNP_241670.1. NC_002570.2.
WP_010896977.1. NC_002570.2.

Genome annotation databases

EnsemblBacteriaiBAB04523; BAB04523; BAB04523.
GeneIDi892178.
KEGGibha:BH0804.
PATRICi18938608. VBIBacHal18977_0842.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB024550 Genomic DNA. Translation: BAA83911.1.
BA000004 Genomic DNA. Translation: BAB04523.1.
PIRiD83750.
RefSeqiNP_241670.1. NC_002570.2.
WP_010896977.1. NC_002570.2.

3D structure databases

ProteinModelPortaliQ9RC88.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272558.BH0804.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB04523; BAB04523; BAB04523.
GeneIDi892178.
KEGGibha:BH0804.
PATRICi18938608. VBIBacHal18977_0842.

Phylogenomic databases

eggNOGiCOG1995.
HOGENOMiHOG000040097.
KOiK00097.
OMAiTHGDING.
OrthoDBiEOG6GN6ZC.

Enzyme and pathway databases

UniPathwayiUPA00244; UER00312.
BioCyciBHAL272558:GJC5-862-MONOMER.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_00536. PdxA.
InterProiIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamiPF04166. PdxA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00557. pdxA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic analysis of the chromosome of alkaliphilic Bacillus halodurans C-125."
    Takami H., Takaki Y., Nakasone K., Sakiyama T., Maeno G., Sasaki R., Hirama C., Fuji F., Masui N.
    Extremophiles 3:227-233(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.
  2. "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
    Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
    Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Entry informationi

Entry nameiPDXA_BACHD
AccessioniPrimary (citable) accession number: Q9RC88
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: May 1, 2000
Last modified: January 7, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site is located at the dimer interface.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.