Reviewed, UniProtKB/Swiss-Prot Q9RC53 (CITS_BACHD)

Names and origin

Protein names

Recommended name:
    Sensor protein citS
    EC=2.7.13.3

Gene names

Name:	citS
Ordered Locus Names:	BH3839

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Member of the two-component regulatory system citT/citS. Functions probably as a membrane-associated protein kinase that phosphorylates citT in response to environmental citrate or Mg2+-citrate complex By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Contains 1 histidine kinase domain. Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 538	538	Sensor protein citS		PRO_0000074734
Regions
	Topological domain	1 – 13	13	Cytoplasmic Potential
	Transmembrane	14 – 34	21	Potential
	Topological domain	35 – 174	140	Extracellular Potential
	Transmembrane	175 – 195	21	Potential
	Topological domain	196 – 538	343	Cytoplasmic Potential
	Domain	216 – 282	67	PAS
	Domain	339 – 534	196	Histidine kinase
Amino acid modifications
	Modified residue	342	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9RC53-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: A679B7A0D7662668	FASTA	538	59,814
	10 20 30 40 50 60 MKRRLFPLTF SAKMMGFIAL LIIAMFVLLG VFLNEQYART LEEQMGERAL SVAQAVALIP 70 80 90 100 110 120 ELREAFSAER PDEIIQPIAE SIRVETGAEF IVVGNTDLIR YAHPLPERIG QRMVGGDNER 130 140 150 160 170 180 ALVHGESYVS KAVGSLGPSI RGKVPVFDDN GKIIGIVSVG FLMEDIQQVI GERLIAMWQI 190 200 210 220 230 240 VVVIMILGLM GTWLVANTVK KATLGLEPEE IGQQFQQKEA ILQSIHEGVI AVNKEGKVTL 250 260 270 280 290 300 FNQAAMKYVD PELDKEDVLG RHVTDLVKHT RLPEVLQVGK GQYDQELRIG DKQAVVNRVP 310 320 330 340 350 360 IYYDHEIVGA VATFRDRNEI KKLSEELTNV KNYADALRAQ THEFSNKLNT ISGFLQLGKI 370 380 390 400 410 420 DEAVDFIQKE RKIQQEWIHF FIERVNDPTV SAVLLGKISQ AQELGIDVDI DPSSQLLTPL 430 440 450 460 470 480 QERQQELLVT MIGNLLENAF DALLASGIEN KKIYISFTDM GDDFIFEVED NGPGIPPQLM 490 500 510 520 530 ESIFEEGFST KEGTHRGFGL ALVKKAVHEL GGAIFLEEGE LGGACFVLTI PKHEAKEG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic analysis of the chromosome of alkaliphilic Bacillus halodurans C-125." Takami H., Takaki Y., Nakasone K., Sakiyama T., Maeno G., Sasaki R., Hirama C., Fuji F., Masui N. Extremophiles 3:227-233(1999) [PubMed: 10484179] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.
[2]	"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB024561 Genomic DNA. Translation: BAA83946.1. BA000004 Genomic DNA. Translation: BAB07558.1.
PIR	G84129.
RefSeq	NP_244706.1.
3D structure databases
HSSP	HSSP built from PDB template 1ID0 based on UniProtKB P23837.
ModBase	Search...
Genome annotation databases
GeneID	891422.
GenomeReviews	Gene locus BH3839 in contig BA000004_GR.
KEGG	bha:BH3839.
NMPDR	fig|272558.1.peg.3839.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9RC53.
OMA	LMESIFE.
Enzyme and pathway databases
BioCyc	BHAL272558:BH3839-MON.
BRENDA	2.7.13.3. 191865.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR000014. PAS. IPR013767. PAS_fold. IPR004358. Sig_transdc_His_kin-like_C. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF02518. HATPase_c. 1 hit. PF00989. PAS. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
PROSITE	PS50109. HIS_KIN. 1 hit. PS50112. PAS. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CITS_BACHD

Accession

Primary (citable) accession number: Q9RC53

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)