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Protein

Putative cellulose synthase 2

Gene

bcsABII-A

Organism
Komagataeibacter xylinus (Gluconacetobacter xylinus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).

Pathwayi: bacterial cellulose biosynthesis

This protein is involved in the pathway bacterial cellulose biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway bacterial cellulose biosynthesis and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei186Sequence analysis1
Binding sitei233SubstrateSequence analysis1
Binding sitei235SubstrateSequence analysis1
Active sitei330Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cellulose biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00694.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative cellulose synthase 2
Including the following 2 domains:
Cellulose synthase catalytic subunit [UDP-forming] (EC:2.4.1.12)
Cyclic di-GMP-binding domain
Alternative name(s):
Cellulose synthase 2 regulatory subunit
Gene namesi
Name:bcsABII-A
OrganismiKomagataeibacter xylinus (Gluconacetobacter xylinus)
Taxonomic identifieri28448 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeKomagataeibacter

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei24 – 44HelicalSequence analysisAdd BLAST21
Transmembranei71 – 91HelicalSequence analysisAdd BLAST21
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
Transmembranei404 – 424HelicalSequence analysisAdd BLAST21
Transmembranei427 – 447HelicalSequence analysisAdd BLAST21
Transmembranei465 – 485HelicalSequence analysisAdd BLAST21
Transmembranei514 – 534HelicalSequence analysisAdd BLAST21
Transmembranei543 – 563HelicalSequence analysisAdd BLAST21
Transmembranei1481 – 1501HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000592651 – 1518Putative cellulose synthase 2Add BLAST1518

Proteomic databases

PRIDEiQ9RBJ2.

Structurei

3D structure databases

ProteinModelPortaliQ9RBJ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini569 – 668PilZAdd BLAST100

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 731CatalyticAdd BLAST731
Regioni144 – 237Catalytic subdomain AAdd BLAST94
Regioni314 – 374Catalytic subdomain BAdd BLAST61
Regioni732 – 1518Cyclic di-GMP bindingBy similarityAdd BLAST787

Domaini

There are two conserved domains in the globular part of the catalytic subunit: the N-terminal domain (domain A) contains the conserved DXD motif and is possibly involved in catalysis and substrate binding. The C-terminal domain (domain B) contains the QXXRW motif and is present only in processive glycosyl transferases. It could be involved in the processivity function of the enzyme, possibly required for holding the growing glycan chain in the active site.

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 2 family.Curated
In the C-terminal section; belongs to the AcsB/BcsB family.Curated
Contains 1 PilZ domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003919. Cell_synth_A.
IPR003920. Cell_synth_B.
IPR018513. Cell_synthase_bac.
IPR005150. Cellulose_synth.
IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR009875. PilZ_domain.
[Graphical view]
PfamiPF03170. BcsB. 1 hit.
PF03552. Cellulose_synt. 1 hit.
PF00535. Glycos_transf_2. 1 hit.
PF07238. PilZ. 1 hit.
[Graphical view]
PRINTSiPR01440. CELLSNTHASEB.
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR03030. CelA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9RBJ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYGTWFTTGK VTDLLARTGL DRVPVWVPVV LGVVLMAFVG SVRIDPALQG
60 70 80 90 100
WVSVGTVTLL LVLNRRRGRG ITVFLMMLSL LVSLRYIVWR LTATVQFSNW
110 120 130 140 150
LQTALAVLLL LAEAYALMTL CLSYFQMAWP LRRREHPLPE DMAQWPSVDV
160 170 180 190 200
FVPSYNEELS LVRSTVLGAL DLDWPADRLN VYILDDGRRK AFHDFAVEAG
210 220 230 240 250
AGYIIRAENN HAKAGNLNHA LAVTDSPFAV IFDCDHVPTR GFLRRTIGWM
260 270 280 290 300
MADPNLALLQ TPHHFYAPDP FQRNLAGGMH VPPEGNMFYG LVQDGNDFWD
310 320 330 340 350
ATFFCGSCAI IRREAVMGIG GFATETVTED AHTALKMQRR GWGTAYLREP
360 370 380 390 400
LAAGLATERL ILHIGQRVRW ARGMIQIMRL DNPMLGAGLR WEQRLCYLSA
410 420 430 440 450
MSHFLFAIPR LTFLVSPLAF LFLGQNIIAA SPLAISVYAL PHIFHSVITL
460 470 480 490 500
SRIEGRWRYS FWSEIYETSL ALFLVRITIV TLLQPHKGKF NVTDKGGLLA
510 520 530 540 550
RGYFDWDAVY PNVILAGVLC AALLRGVFGI VWQFHDRLAL QSFILNTLWV
560 570 580 590 600
VISLIIVLAS IAVGRETRQT RNAPRVSVRL PVVVTDAHGR QMEGHTHDIS
610 620 630 640 650
LGGLAVGTRL ATPDMVGGEV TVRYDSARDG IHVGVPARVL DARDGTLRLR
660 670 680 690 700
WAVRDLEDER QVVSMVFGRN DAWAGWADFA PDRPLRSLAM VFRSIGGLLR
710 720 730 740 750
RRPAEAPRAL HEMGEGELPA TEEKLEKQSF VLKPVPRSAR HGATASAALF
760 770 780 790 800
VAFTALVPAA MAQEAPSPDQ SGVTAETPFG DSNTGVVPDA LPAIDPAVAD
810 820 830 840 850
RISDAEVTRT LTFRNLGATT GPLTLRGYSP LQGLDVVVPA NRVVTHAQLT
860 870 880 890 900
LSGALSPSLL PEASAVTVTL NEQYVGTLKV DPQHPQFGPV SFDIDPLYFT
910 920 930 940 950
GDNKLNFHFA GEYRRDCNDL FNEILWARIS DMSRITLTTV RITPERKLSR
960 970 980 990 1000
LPAPFFDPNQ RSTLRVPVVL PATGDRGALR AAGLVASWFG RIADFRKLSF
1010 1020 1030 1040 1050
PVSTTIPASG NAVEVGVNLP VDAEGGRPAG PMLAEVANPN DRWGTVLVVT
1060 1070 1080 1090 1100
GRTAQEVEVA ARALVFSPDT LGGVASKVVS DVSLETRHPY DAPAFVPTDR
1110 1120 1130 1140 1150
PVRFGELVGA ADLQGGGFAP AGMTLPFHLP PDLYTWRGRP FLMNMWVRAP
1160 1170 1180 1190 1200
GGPVVDLETS RVDVSLNNNY LQSYTLSPPG LWRKWSERLV NQHAGAVGHV
1210 1220 1230 1240 1250
TALPPWLLFG QNQLQFNFDA RPIDRGACRR TPGDIHMSVD SDSTLDFRRG
1260 1270 1280 1290 1300
YHFAEMPNLS YFAEAAFPFS RMADLSETTV VLPDHPDTGT TGAFLDLMGF
1310 1320 1330 1340 1350
FGASTWYPAA GVTVMGADEV AHTPPKGDIV VLGTAAQLGG AASGLLARSP
1360 1370 1380 1390 1400
YVIHDRHITV GQRMGLQGIW YLFQDHDHAG LKDGVTANLN APIAEAGVLL
1410 1420 1430 1440 1450
AAQSPYDSQR SVVAFTGDTP ERIHDLVLSL RNKGDLPSLQ GDLVLKNGDR
1460 1470 1480 1490 1500
FTSYRTAPVY TVGSLPLWLR LDWFLGHHPS ALYLAGLAGA GLAALGVWAW
1510
LRGWSRKRIA RDDLTGEL
Length:1,518
Mass (Da):166,405
Last modified:May 1, 2000 - v1
Checksum:i0EC99B35B6DE4543
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015803 Genomic DNA. Translation: BAA77593.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015803 Genomic DNA. Translation: BAA77593.1.

3D structure databases

ProteinModelPortaliQ9RBJ2.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Proteomic databases

PRIDEiQ9RBJ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00694.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003919. Cell_synth_A.
IPR003920. Cell_synth_B.
IPR018513. Cell_synthase_bac.
IPR005150. Cellulose_synth.
IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR009875. PilZ_domain.
[Graphical view]
PfamiPF03170. BcsB. 1 hit.
PF03552. Cellulose_synt. 1 hit.
PF00535. Glycos_transf_2. 1 hit.
PF07238. PilZ. 1 hit.
[Graphical view]
PRINTSiPR01440. CELLSNTHASEB.
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR03030. CelA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBCSA4_KOMXY
AccessioniPrimary (citable) accession number: Q9RBJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: May 1, 2000
Last modified: October 5, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.