Reviewed, UniProtKB/Swiss-Prot Q9RBJ0 (ISPH_ACIAD)

Names and origin

Protein names

Recommended name:
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
    EC=1.17.1.2

Gene names

Name:	ispH
Synonyms:	lytB
Ordered Locus Names:	ACIAD3322

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the ispH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_0000128762

Sequences

	Sequence		Length	Mass (Da)
	Q9RBJ0-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 29F071CEF2B9A762	FASTA	316	34,944
	10 20 30 40 50 60 MEIVLANPRG FCAGVDRAIA IVNRALECFN PPIYVRHEVV HNKFVVDDLR QRGAIFVDEL 70 80 90 100 110 120 DQVPDDSIVI FSAHGVSKAV QQEAEHRGLK VFDATCPLVT KVHIEVTKYA REGTEAILIG 130 140 150 160 170 180 HEGHPEVEGT MGQYDKSKGG HIYLVEDEAD VEALAVNHPE KLAFVTQTTL SIDDTAKVID 190 200 210 220 230 240 ALRTKFPQIQ GPRKDDICYA TQNRQDAVRD LASRCDVVLV VGSPNSSNSN RLRELAERMG 250 260 270 280 290 300 KAAYLVDNAD QLEQQWFDGV AKIGVTAGAS APEILIKQVI QRLQDWGAEA PKELDGREEN 310 ITFSLPKELR IQVTQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ComB, a novel competence gene required for natural transformation of Acinetobacter sp. BD413: identification, characterization and analysis of growth phase-dependent regulation." Herzberg C., Friedrich A., Averhoff B. Arch. Microbiol. 173:220-228(2000) [PubMed: 10763755] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF027189 Genomic DNA. Translation: AAD55804.1. CR543861 Genomic DNA. Translation: CAG69994.1.
RefSeq	YP_047816.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9RBJ0.
Genome annotation databases
GeneID	2879847.
GenomeReviews	Gene locus ACIAD3322 in contig CR543861_GR.
KEGG	aci:ACIAD3322.
NMPDR	fig|62977.3.peg.3009.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG335228.
OMA	HMQVARA.
PhylomeDB	Q9RBJ0.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD3322-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. ispH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_ACIAD

Accession

Primary (citable) accession number: Q9RBJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	May 1, 2000
Last modified:	February 9, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)