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Q9RAM7

- TYSY_METFK

UniProt

Q9RAM7 - TYSY_METFK

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Protein
Thymidylate synthase
Gene
thyA1, Mfla_0906
thyA2, Mfla_1050
Organism
Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei146 – 1461 By similarity

GO - Molecular functioni

  1. thymidylate synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. dTMP biosynthetic process Source: UniProtKB-HAMAP
  2. dTTP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

BioCyciMFLA265072:GHWJ-1073-MONOMER.
MFLA265072:GHWJ-929-MONOMER.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
Name:thyA1
Ordered Locus Names:Mfla_0906
AND
Name:thyA2
Ordered Locus Names:Mfla_1050
OrganismiMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
Taxonomic identifieri265072 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaMethylophilalesMethylophilaceaeMethylobacillus
ProteomesiUP000002440: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264Thymidylate synthaseUniRule annotation
PRO_0000140981Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi265072.Mfla_1050.

Structurei

3D structure databases

ProteinModelPortaliQ9RAM7.
SMRiQ9RAM7. Positions 1-264.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257900.
KOiK00560.
OMAiDEWARDD.
OrthoDBiEOG6K6V53.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9RAM7-1 [UniParc]FASTAAdd to Basket

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MKVYHDLMRH VLEQGHKKED RTGTGTLSVF GYQMRFDLSE GFPLLTTKKV    50
HLKSIIHELL WFLQGSTNIA YLKENGVTIW DEWADAEGNL GPVYGYQWRN 100
WPKPDGGHID QISEVIAAIK RNPDSRRLIV SAWNVADVDK MKLPPCHAFF 150
QFYVADGKLS CQLYQRSADI FLGVPFNIAS YALLTMMVAQ VCDLKLGDFV 200
HTLGDAHIYL NHLEQVKEQL SREPYPLPVM RINPEVKDIF AFRFEDFTLE 250
NYQSHPAIKA PVAV 264
Length:264
Mass (Da):30,282
Last modified:October 3, 2006 - v2
Checksum:iB2FF060956C70D40
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 542KS → NA in AAF21131. 1 Publication
Sequence conflicti121 – 1211R → S in AAF21131. 1 Publication
Sequence conflicti142 – 1421K → E in AAF21131. 1 Publication
Sequence conflicti149 – 1491F → L in AAF21131. 1 Publication
Sequence conflicti156 – 1561D → E in AAF21131. 1 Publication
Sequence conflicti190 – 1901Q → R in AAF21131. 1 Publication
Sequence conflicti195 – 1951K → E in AAF21131. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L78665 Genomic DNA. Translation: AAF21131.1.
CP000284 Genomic DNA. Translation: ABE49318.1.
CP000284 Genomic DNA. Translation: ABE49174.1.
RefSeqiYP_545015.1. NC_007947.1.
YP_545159.1. NC_007947.1.

Genome annotation databases

EnsemblBacteriaiABE49174; ABE49174; Mfla_0906.
ABE49318; ABE49318; Mfla_1050.
GeneIDi4000762.
4001311.
KEGGimfa:Mfla_0906.
mfa:Mfla_1050.
PATRICi32267431. VBIMetFla97085_0953.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L78665 Genomic DNA. Translation: AAF21131.1 .
CP000284 Genomic DNA. Translation: ABE49318.1 .
CP000284 Genomic DNA. Translation: ABE49174.1 .
RefSeqi YP_545015.1. NC_007947.1.
YP_545159.1. NC_007947.1.

3D structure databases

ProteinModelPortali Q9RAM7.
SMRi Q9RAM7. Positions 1-264.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 265072.Mfla_1050.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABE49174 ; ABE49174 ; Mfla_0906 .
ABE49318 ; ABE49318 ; Mfla_1050 .
GeneIDi 4000762.
4001311.
KEGGi mfa:Mfla_0906.
mfa:Mfla_1050.
PATRICi 32267431. VBIMetFla97085_0953.

Phylogenomic databases

eggNOGi COG0207.
HOGENOMi HOG000257900.
KOi K00560.
OMAi DEWARDD.
OrthoDBi EOG6K6V53.

Enzyme and pathway databases

UniPathwayi UPA00575 .
BioCyci MFLA265072:GHWJ-1073-MONOMER.
MFLA265072:GHWJ-929-MONOMER.

Family and domain databases

Gene3Di 3.30.572.10. 1 hit.
HAMAPi MF_00008. Thymidy_synth_bact.
InterProi IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view ]
Pfami PF00303. Thymidylat_synt. 1 hit.
[Graphical view ]
PRINTSi PR00108. THYMDSNTHASE.
SUPFAMi SSF55831. SSF55831. 1 hit.
TIGRFAMsi TIGR03284. thym_sym. 2 hits.
PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Organization of threonine biosynthesis genes from the obligate methylotroph Methylobacillus flagellatus."
    Marchenko G.N., Marchenko N.D., Tsygankov Y.D., Chistoserdov A.Y.
    Microbiology 145:3273-3282(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KT / ATCC 51484 / DSM 6875.

Entry informationi

Entry nameiTYSY_METFK
AccessioniPrimary (citable) accession number: Q9RAM7
Secondary accession number(s): Q1H2G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: October 3, 2006
Last modified: May 14, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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