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Q9RAM7

- TYSY_METFK

UniProt

Q9RAM7 - TYSY_METFK

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Protein

Thymidylate synthase

Gene

thyA1

more
Organism
Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei146 – 1461UniRule annotation

GO - Molecular functioni

  1. thymidylate synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. dTMP biosynthetic process Source: UniProtKB-HAMAP
  2. dTTP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

BioCyciMFLA265072:GHWJ-1073-MONOMER.
MFLA265072:GHWJ-929-MONOMER.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyA1UniRule annotation
Ordered Locus Names:Mfla_0906
AND
Name:thyA2UniRule annotation
Ordered Locus Names:Mfla_1050
OrganismiMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
Taxonomic identifieri265072 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaMethylophilalesMethylophilaceaeMethylobacillus
ProteomesiUP000002440: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264Thymidylate synthasePRO_0000140981Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi265072.Mfla_1050.

Structurei

3D structure databases

ProteinModelPortaliQ9RAM7.
SMRiQ9RAM7. Positions 1-264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257900.
KOiK00560.
OMAiDEWARDD.
OrthoDBiEOG6K6V53.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9RAM7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVYHDLMRH VLEQGHKKED RTGTGTLSVF GYQMRFDLSE GFPLLTTKKV
60 70 80 90 100
HLKSIIHELL WFLQGSTNIA YLKENGVTIW DEWADAEGNL GPVYGYQWRN
110 120 130 140 150
WPKPDGGHID QISEVIAAIK RNPDSRRLIV SAWNVADVDK MKLPPCHAFF
160 170 180 190 200
QFYVADGKLS CQLYQRSADI FLGVPFNIAS YALLTMMVAQ VCDLKLGDFV
210 220 230 240 250
HTLGDAHIYL NHLEQVKEQL SREPYPLPVM RINPEVKDIF AFRFEDFTLE
260
NYQSHPAIKA PVAV
Length:264
Mass (Da):30,282
Last modified:October 3, 2006 - v2
Checksum:iB2FF060956C70D40
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 542KS → NA in AAF21131. (PubMed:10589737)Curated
Sequence conflicti121 – 1211R → S in AAF21131. (PubMed:10589737)Curated
Sequence conflicti142 – 1421K → E in AAF21131. (PubMed:10589737)Curated
Sequence conflicti149 – 1491F → L in AAF21131. (PubMed:10589737)Curated
Sequence conflicti156 – 1561D → E in AAF21131. (PubMed:10589737)Curated
Sequence conflicti190 – 1901Q → R in AAF21131. (PubMed:10589737)Curated
Sequence conflicti195 – 1951K → E in AAF21131. (PubMed:10589737)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L78665 Genomic DNA. Translation: AAF21131.1.
CP000284 Genomic DNA. Translation: ABE49318.1.
CP000284 Genomic DNA. Translation: ABE49174.1.
RefSeqiWP_011479271.1. NC_007947.1.
YP_545015.1. NC_007947.1.
YP_545159.1. NC_007947.1.

Genome annotation databases

EnsemblBacteriaiABE49174; ABE49174; Mfla_0906.
ABE49318; ABE49318; Mfla_1050.
GeneIDi4000762.
4001311.
KEGGimfa:Mfla_0906.
mfa:Mfla_1050.
PATRICi32267431. VBIMetFla97085_0953.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L78665 Genomic DNA. Translation: AAF21131.1 .
CP000284 Genomic DNA. Translation: ABE49318.1 .
CP000284 Genomic DNA. Translation: ABE49174.1 .
RefSeqi WP_011479271.1. NC_007947.1.
YP_545015.1. NC_007947.1.
YP_545159.1. NC_007947.1.

3D structure databases

ProteinModelPortali Q9RAM7.
SMRi Q9RAM7. Positions 1-264.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 265072.Mfla_1050.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABE49174 ; ABE49174 ; Mfla_0906 .
ABE49318 ; ABE49318 ; Mfla_1050 .
GeneIDi 4000762.
4001311.
KEGGi mfa:Mfla_0906.
mfa:Mfla_1050.
PATRICi 32267431. VBIMetFla97085_0953.

Phylogenomic databases

eggNOGi COG0207.
HOGENOMi HOG000257900.
KOi K00560.
OMAi DEWARDD.
OrthoDBi EOG6K6V53.

Enzyme and pathway databases

UniPathwayi UPA00575 .
BioCyci MFLA265072:GHWJ-1073-MONOMER.
MFLA265072:GHWJ-929-MONOMER.

Family and domain databases

Gene3Di 3.30.572.10. 1 hit.
HAMAPi MF_00008. Thymidy_synth_bact.
InterProi IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view ]
Pfami PF00303. Thymidylat_synt. 1 hit.
[Graphical view ]
PRINTSi PR00108. THYMDSNTHASE.
SUPFAMi SSF55831. SSF55831. 1 hit.
TIGRFAMsi TIGR03284. thym_sym. 2 hits.
PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Organization of threonine biosynthesis genes from the obligate methylotroph Methylobacillus flagellatus."
    Marchenko G.N., Marchenko N.D., Tsygankov Y.D., Chistoserdov A.Y.
    Microbiology 145:3273-3282(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KT / ATCC 51484 / DSM 6875.

Entry informationi

Entry nameiTYSY_METFK
AccessioniPrimary (citable) accession number: Q9RAM7
Secondary accession number(s): Q1H2G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: October 3, 2006
Last modified: October 29, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3