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Q9RAM7 (TYSY_METFK) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA1
Ordered Locus Names:Mfla_0906
AND
Name:thyA2
Ordered Locus Names:Mfla_1050
OrganismMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]
Taxonomic identifier265072 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaMethylophilalesMethylophilaceaeMethylobacillus

Protein attributes

Sequence length264 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 264264Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000140981

Sites

Active site1461 By similarity

Experimental info

Sequence conflict53 – 542KS → NA in AAF21131. Ref.1
Sequence conflict1211R → S in AAF21131. Ref.1
Sequence conflict1421K → E in AAF21131. Ref.1
Sequence conflict1491F → L in AAF21131. Ref.1
Sequence conflict1561D → E in AAF21131. Ref.1
Sequence conflict1901Q → R in AAF21131. Ref.1
Sequence conflict1951K → E in AAF21131. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9RAM7 [UniParc].

Last modified October 3, 2006. Version 2.
Checksum: B2FF060956C70D40

FASTA26430,282
        10         20         30         40         50         60 
MKVYHDLMRH VLEQGHKKED RTGTGTLSVF GYQMRFDLSE GFPLLTTKKV HLKSIIHELL 

        70         80         90        100        110        120 
WFLQGSTNIA YLKENGVTIW DEWADAEGNL GPVYGYQWRN WPKPDGGHID QISEVIAAIK 

       130        140        150        160        170        180 
RNPDSRRLIV SAWNVADVDK MKLPPCHAFF QFYVADGKLS CQLYQRSADI FLGVPFNIAS 

       190        200        210        220        230        240 
YALLTMMVAQ VCDLKLGDFV HTLGDAHIYL NHLEQVKEQL SREPYPLPVM RINPEVKDIF 

       250        260 
AFRFEDFTLE NYQSHPAIKA PVAV 

« Hide

References

« Hide 'large scale' references
[1]"Organization of threonine biosynthesis genes from the obligate methylotroph Methylobacillus flagellatus."
Marchenko G.N., Marchenko N.D., Tsygankov Y.D., Chistoserdov A.Y.
Microbiology 145:3273-3282(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequence of Methylobacillus flagellatus KT."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KT / ATCC 51484 / DSM 6875.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L78665 Genomic DNA. Translation: AAF21131.1.
CP000284 Genomic DNA. Translation: ABE49318.1.
CP000284 Genomic DNA. Translation: ABE49174.1.
RefSeqYP_545015.1. NC_007947.1.
YP_545159.1. NC_007947.1.

3D structure databases

ProteinModelPortalQ9RAM7.
SMRQ9RAM7. Positions 1-264.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING265072.Mfla_1050.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE49174; ABE49174; Mfla_0906.
ABE49318; ABE49318; Mfla_1050.
GeneID4000762.
4001311.
KEGGmfa:Mfla_0906.
mfa:Mfla_1050.
PATRIC32267431. VBIMetFla97085_0953.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257900.
KOK00560.
OMANLGPVYG.
OrthoDBEOG6K6V53.
ProtClustDBPRK01827.

Enzyme and pathway databases

BioCycMFLA265072:GHWJ-1073-MONOMER.
MFLA265072:GHWJ-929-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 2 hits.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_METFK
AccessionPrimary (citable) accession number: Q9RAM7
Secondary accession number(s): Q1H2G9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: October 3, 2006
Last modified: February 19, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways