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Q9RAM7

- TYSY_METFK

UniProt

Q9RAM7 - TYSY_METFK

Protein

Thymidylate synthase

Gene

thyA1

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Organism
Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (03 Oct 2006)
      Previous versions | rss
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    • Comment

    Functioni

    Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei146 – 1461UniRule annotation

    GO - Molecular functioni

    1. thymidylate synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. dTMP biosynthetic process Source: UniProtKB-HAMAP
    2. dTTP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Enzyme and pathway databases

    BioCyciMFLA265072:GHWJ-1073-MONOMER.
    MFLA265072:GHWJ-929-MONOMER.
    UniPathwayiUPA00575.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
    Short name:
    TSUniRule annotation
    Short name:
    TSaseUniRule annotation
    Gene namesi
    Name:thyA1UniRule annotation
    Ordered Locus Names:Mfla_0906
    AND
    Name:thyA2UniRule annotation
    Ordered Locus Names:Mfla_1050
    OrganismiMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
    Taxonomic identifieri265072 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaMethylophilalesMethylophilaceaeMethylobacillus
    ProteomesiUP000002440: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 264264Thymidylate synthasePRO_0000140981Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi265072.Mfla_1050.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9RAM7.
    SMRiQ9RAM7. Positions 1-264.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0207.
    HOGENOMiHOG000257900.
    KOiK00560.
    OMAiDEWARDD.
    OrthoDBiEOG6K6V53.

    Family and domain databases

    Gene3Di3.30.572.10. 1 hit.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 2 hits.
    PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9RAM7-1 [UniParc]FASTAAdd to Basket

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    MKVYHDLMRH VLEQGHKKED RTGTGTLSVF GYQMRFDLSE GFPLLTTKKV    50
    HLKSIIHELL WFLQGSTNIA YLKENGVTIW DEWADAEGNL GPVYGYQWRN 100
    WPKPDGGHID QISEVIAAIK RNPDSRRLIV SAWNVADVDK MKLPPCHAFF 150
    QFYVADGKLS CQLYQRSADI FLGVPFNIAS YALLTMMVAQ VCDLKLGDFV 200
    HTLGDAHIYL NHLEQVKEQL SREPYPLPVM RINPEVKDIF AFRFEDFTLE 250
    NYQSHPAIKA PVAV 264
    Length:264
    Mass (Da):30,282
    Last modified:October 3, 2006 - v2
    Checksum:iB2FF060956C70D40
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti53 – 542KS → NA in AAF21131. (PubMed:10589737)Curated
    Sequence conflicti121 – 1211R → S in AAF21131. (PubMed:10589737)Curated
    Sequence conflicti142 – 1421K → E in AAF21131. (PubMed:10589737)Curated
    Sequence conflicti149 – 1491F → L in AAF21131. (PubMed:10589737)Curated
    Sequence conflicti156 – 1561D → E in AAF21131. (PubMed:10589737)Curated
    Sequence conflicti190 – 1901Q → R in AAF21131. (PubMed:10589737)Curated
    Sequence conflicti195 – 1951K → E in AAF21131. (PubMed:10589737)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L78665 Genomic DNA. Translation: AAF21131.1.
    CP000284 Genomic DNA. Translation: ABE49318.1.
    CP000284 Genomic DNA. Translation: ABE49174.1.
    RefSeqiYP_545015.1. NC_007947.1.
    YP_545159.1. NC_007947.1.

    Genome annotation databases

    EnsemblBacteriaiABE49174; ABE49174; Mfla_0906.
    ABE49318; ABE49318; Mfla_1050.
    GeneIDi4000762.
    4001311.
    KEGGimfa:Mfla_0906.
    mfa:Mfla_1050.
    PATRICi32267431. VBIMetFla97085_0953.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L78665 Genomic DNA. Translation: AAF21131.1 .
    CP000284 Genomic DNA. Translation: ABE49318.1 .
    CP000284 Genomic DNA. Translation: ABE49174.1 .
    RefSeqi YP_545015.1. NC_007947.1.
    YP_545159.1. NC_007947.1.

    3D structure databases

    ProteinModelPortali Q9RAM7.
    SMRi Q9RAM7. Positions 1-264.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 265072.Mfla_1050.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABE49174 ; ABE49174 ; Mfla_0906 .
    ABE49318 ; ABE49318 ; Mfla_1050 .
    GeneIDi 4000762.
    4001311.
    KEGGi mfa:Mfla_0906.
    mfa:Mfla_1050.
    PATRICi 32267431. VBIMetFla97085_0953.

    Phylogenomic databases

    eggNOGi COG0207.
    HOGENOMi HOG000257900.
    KOi K00560.
    OMAi DEWARDD.
    OrthoDBi EOG6K6V53.

    Enzyme and pathway databases

    UniPathwayi UPA00575 .
    BioCyci MFLA265072:GHWJ-1073-MONOMER.
    MFLA265072:GHWJ-929-MONOMER.

    Family and domain databases

    Gene3Di 3.30.572.10. 1 hit.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 2 hits.
    PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Organization of threonine biosynthesis genes from the obligate methylotroph Methylobacillus flagellatus."
      Marchenko G.N., Marchenko N.D., Tsygankov Y.D., Chistoserdov A.Y.
      Microbiology 145:3273-3282(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: KT / ATCC 51484 / DSM 6875.

    Entry informationi

    Entry nameiTYSY_METFK
    AccessioniPrimary (citable) accession number: Q9RAM7
    Secondary accession number(s): Q1H2G9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 25, 2003
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3