Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thymidylate synthase

Gene

thyA1

more
Organism
Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21dUMPUniRule annotation1
Binding sitei515,10-methylenetetrahydrofolateUniRule annotation1
Active sitei146NucleophileUniRule annotation1
Binding sitei1695,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei177dUMPUniRule annotation1
Binding sitei2635,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi126 – 127dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi166 – 169dUMPUniRule annotation4
Nucleotide bindingi207 – 209dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyA1UniRule annotation
Ordered Locus Names:Mfla_0906
AND
Name:thyA2UniRule annotation
Ordered Locus Names:Mfla_1050
OrganismiMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
Taxonomic identifieri265072 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesMethylophilaceaeMethylobacillus
Proteomesi
  • UP000002440 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409811 – 264Thymidylate synthaseAdd BLAST264

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi265072.Mfla_1050.

Structurei

3D structure databases

ProteinModelPortaliQ9RAM7.
SMRiQ9RAM7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V. Bacteria.
COG0207. LUCA.
HOGENOMiHOG000257900.
KOiK00560.
OMAiKQYLDLC.
OrthoDBiPOG091H02L6.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiView protein in InterPro
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
PfamiView protein in Pfam
PF00303. Thymidylat_synt. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
PROSITEiView protein in PROSITE
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9RAM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVYHDLMRH VLEQGHKKED RTGTGTLSVF GYQMRFDLSE GFPLLTTKKV
60 70 80 90 100
HLKSIIHELL WFLQGSTNIA YLKENGVTIW DEWADAEGNL GPVYGYQWRN
110 120 130 140 150
WPKPDGGHID QISEVIAAIK RNPDSRRLIV SAWNVADVDK MKLPPCHAFF
160 170 180 190 200
QFYVADGKLS CQLYQRSADI FLGVPFNIAS YALLTMMVAQ VCDLKLGDFV
210 220 230 240 250
HTLGDAHIYL NHLEQVKEQL SREPYPLPVM RINPEVKDIF AFRFEDFTLE
260
NYQSHPAIKA PVAV
Length:264
Mass (Da):30,282
Last modified:October 3, 2006 - v2
Checksum:iB2FF060956C70D40
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53 – 54KS → NA in AAF21131 (PubMed:10589737).Curated2
Sequence conflicti121R → S in AAF21131 (PubMed:10589737).Curated1
Sequence conflicti142K → E in AAF21131 (PubMed:10589737).Curated1
Sequence conflicti149F → L in AAF21131 (PubMed:10589737).Curated1
Sequence conflicti156D → E in AAF21131 (PubMed:10589737).Curated1
Sequence conflicti190Q → R in AAF21131 (PubMed:10589737).Curated1
Sequence conflicti195K → E in AAF21131 (PubMed:10589737).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L78665 Genomic DNA. Translation: AAF21131.1.
CP000284 Genomic DNA. Translation: ABE49318.1.
CP000284 Genomic DNA. Translation: ABE49174.1.
RefSeqiWP_011479271.1. NC_007947.1.

Genome annotation databases

EnsemblBacteriaiABE49174; ABE49174; Mfla_0906.
ABE49318; ABE49318; Mfla_1050.
KEGGimfa:Mfla_0906.
mfa:Mfla_1050.

Similar proteinsi

Entry informationi

Entry nameiTYSY_METFK
AccessioniPrimary (citable) accession number: Q9RAM7
Secondary accession number(s): Q1H2G9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: October 3, 2006
Last modified: August 30, 2017
This is version 95 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families