Q9RAM6 (KHSE_METFK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	Mfla_0361

Organism

Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]

Taxonomic identifier

265072 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Methylophilales › Methylophilaceae › Methylobacillus

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00301
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00301
Sequence similarities	Belongs to the pseudomonas-type thrB family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Homoserine kinase HAMAP MF_00301		PRO_0000172190
Experimental info
	Sequence conflict	273 – 274	2	WL → CV in AAF21132. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9RAM6 [UniParc]. Last modified October 3, 2006. Version 2. Checksum: 665D6F38E406891B	FASTA	319	35,697
	10 20 30 40 50 60 MSVFTTVSFE QMQQWLKGYD LGELLDLQGI ASGITNTNYF VTTDNGRYVL TLFEEHSAEE 70 80 90 100 110 120 LPNFLDLMTH LAERGIPCPH PVKNNAGRAL GELNGKPAAL VSCLAGRSLD NPMPQHCAAI 130 140 150 160 170 180 GEVLARMHIA GASFKAGMSN LRGQEWRIAT AAKVAPFLDE ENHRMLDAQL EFERTFDTRR 190 200 210 220 230 240 LPRGVIHADL FRDNVLMDGD KVGGVIDFYY ACHDALLYDI AIAVNDWCVN ADCTLDAVRV 250 260 270 280 290 300 RAFLDAYHAI RPLTGEEHAA WPGMLRVAAM RFWLSRLNDL YFPQAGELTH AKDPAYFERI 310 LKHSIAAREQ ILAVWVNAH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization of threonine biosynthesis genes from the obligate methylotroph Methylobacillus flagellatus." Marchenko G.N., Marchenko N.D., Tsygankov Y.D., Chistoserdov A.Y. Microbiology 145:3273-3282(1999) [PubMed: 10589737] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Methylobacillus flagellatus KT." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT / ATCC 51484 / DSM 6875.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L78666 Genomic DNA. Translation: AAF21132.1. CP000284 Genomic DNA. Translation: ABE48632.1.
RefSeq	YP_544473.1. NC_007947.1.
3D structure databases
ProteinModelPortal	Q9RAM6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9RAM6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3999289.
GenomeReviews	Gene locus Mfla_0361 in contig CP000284_GR.
KEGG	mfa:Mfla_0361.
NMPDR	fig|265072.7.peg.361.
PATRIC	32266251. VBIMetFla97085_0368.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2334.
HOGENOM	HBG309377.
OMA	ICLNAWC.
ProtClustDB	PRK05231.
Enzyme and pathway databases
BioCyc	MFLA265072:MFLA_0361-MONOMER.
Family and domain databases
HAMAP	MF_00301. Homoser_kinase_2. [Tree]
InterPro	IPR002575. Aminoglycoside_PTrfase. IPR005280. Homoserine_kinase_ThrB. IPR011009. Kinase-like_dom. [Graphical view]
KO	K02204.
Pfam	PF01636. APH. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
TIGRFAMs	TIGR00938. ThrB_alt. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KHSE_METFK

Accession

Primary (citable) accession number: Q9RAM6
Secondary accession number(s): Q1H4F5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	October 3, 2006
Last modified:	January 25, 2012
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program