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Protein

Bifunctional purine biosynthesis protein PurH

Gene

purH

Organism
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional purine biosynthesis protein PurH (purH)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional purine biosynthesis protein PurH (purH)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Transferase
Biological processPurine biosynthesis

Enzyme and pathway databases

BioCyciMAVI262316:G1G0E-947-MONOMER
UniPathwayiUPA00074; UER00133
UPA00074; UER00135

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthesis protein PurHUniRule annotation
Including the following 2 domains:
Phosphoribosylaminoimidazolecarboxamide formyltransferaseUniRule annotation (EC:2.1.2.3UniRule annotation)
Alternative name(s):
AICAR transformylaseUniRule annotation
IMP cyclohydrolaseUniRule annotation (EC:3.5.4.10UniRule annotation)
Alternative name(s):
ATICUniRule annotation
IMP synthaseUniRule annotation
InosinicaseUniRule annotation
Gene namesi
Name:purHUniRule annotation
Ordered Locus Names:MAP_0903
OrganismiMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Taxonomic identifieri262316 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
Proteomesi
  • UP000000580 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001921051 – 527Bifunctional purine biosynthesis protein PurHAdd BLAST527

Interactioni

Protein-protein interaction databases

STRINGi262316.MAP0903

Structurei

3D structure databases

ProteinModelPortaliQ9RAJ5
SMRiQ9RAJ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 156MGS-likePROSITE-ProRule annotationAdd BLAST148

Domaini

The IMP cyclohydrolase activity resides in the N-terminal region.UniRule annotation

Sequence similaritiesi

Belongs to the PurH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DC1 Bacteria
COG0138 LUCA
KOiK00602
OMAiDLLFAWK
OrthoDBiPOG091H00UT

Family and domain databases

Gene3Di3.40.140.20, 2 hits
3.40.50.1380, 1 hit
HAMAPiMF_00139 PurH, 1 hit
InterProiView protein in InterPro
IPR024051 AICAR_Tfase_dup_dom_sf
IPR016193 Cytidine_deaminase-like
IPR011607 MGS-like_dom
IPR036914 MGS-like_dom_sf
IPR002695 PurH-like
PANTHERiPTHR11692 PTHR11692, 1 hit
PfamiView protein in Pfam
PF01808 AICARFT_IMPCHas, 1 hit
PF02142 MGS, 1 hit
PIRSFiPIRSF000414 AICARFT_IMPCHas, 1 hit
SMARTiView protein in SMART
SM00798 AICARFT_IMPCHas, 1 hit
SM00851 MGS, 1 hit
SUPFAMiSSF52335 SSF52335, 1 hit
SSF53927 SSF53927, 1 hit
TIGRFAMsiTIGR00355 purH, 1 hit
PROSITEiView protein in PROSITE
PS51855 MGS, 1 hit

Sequencei

Sequence statusi: Complete.

Q9RAJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDDWRENA KRPIRRALIS VYDKTGLVEL AQGLTEAGVE IVSTGSTAKV
60 70 80 90 100
IAEKGIPVTR VEVLTGFPEV LDGRVKTLHP RVHAGLLADL RKPEHAAALE
110 120 130 140 150
QLGIAAFELV VVNLYPFTET VDSGAGIDEC VEQIDIGGPS MVRAAAKNHP
160 170 180 190 200
SVAVVVDPLG YDGVLAAVRH GGFTLAERKR LAALAFQHTA DYDIAVATWM
210 220 230 240 250
ESTLAPEHPP TTFPKWLGRS WRRSAMLRYG ENPHQQASLY SDPGAWPGLA
260 270 280 290 300
QAEQLHGKEM SYNNFTDADA AWRAAFDHEQ TCVAIIKHAN PCGIAISSIS
310 320 330 340 350
VADAHRKAHE CDPLSAFGGV IAANTEVSVE MAEYVSTIFT EVIIAPAYQP
360 370 380 390 400
AALEILTRKK NIRVLVASEP LTGGTELRPI SGGLLVQQRD ELDAHGDNPA
410 420 430 440 450
NWTLATGAPA DPATLADLVF AWRVCRAVKS NAIVIAAGGA TIGVGMGQVN
460 470 480 490 500
RVDAARLAVE RGGDRVRGAV AASDAFFPFP DGLETLTGAG VKAVVHPGGS
510 520
VRDDEVTAAA ANAGITLYLT GARHFAH
Length:527
Mass (Da):55,888
Last modified:March 15, 2004 - v2
Checksum:i8B4459CFB40EC338
GO

Sequence cautioni

The sequence AAS03220 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti63V → E in AAF05727 (Ref. 1) Curated1
Sequence conflicti522A → V in AAF05727 (Ref. 1) Curated1
Sequence conflicti526A → V in AAF05727 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF191543 Genomic DNA Translation: AAF05727.1
AE016958 Genomic DNA Translation: AAS03220.1 Different initiation.
RefSeqiWP_040963270.1, NC_002944.2

Genome annotation databases

EnsemblBacteriaiAAS03220; AAS03220; MAP_0903
GeneIDi2718397
KEGGimpa:MAP_0903
PATRICifig|262316.17.peg.944

Similar proteinsi

Entry informationi

Entry nameiPUR9_MYCPA
AccessioniPrimary (citable) accession number: Q9RAJ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 15, 2004
Last modified: March 28, 2018
This is version 101 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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