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Q9RAJ5 (PUR9_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

  1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
    EC=2.1.2.3
    Alternative name(s):
    AICAR transformylase
  2. IMP cyclohydrolase
    EC=3.5.4.10
    Alternative name(s):
    ATIC
    IMP synthase
    Inosinicase
Gene names
Name:purH
Ordered Locus Names:MAP_0903
OrganismMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10) [Complete proteome] [HAMAP]
Taxonomic identifier262316 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)

Protein attributes

Sequence length527 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP-Rule MF_00139

Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.

Domain

The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP-Rule MF_00139

Sequence similarities

Belongs to the PurH family.

Sequence caution

The sequence AAS03220.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 527527Bifunctional purine biosynthesis protein PurH HAMAP-Rule MF_00139
PRO_0000192105

Experimental info

Sequence conflict631V → E in AAF05727. Ref.1
Sequence conflict5221A → V in AAF05727. Ref.1
Sequence conflict5261A → V in AAF05727. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9RAJ5 [UniParc].

Last modified March 15, 2004. Version 2.
Checksum: 8B4459CFB40EC338

FASTA52755,888
        10         20         30         40         50         60 
MSTDDWRENA KRPIRRALIS VYDKTGLVEL AQGLTEAGVE IVSTGSTAKV IAEKGIPVTR 

        70         80         90        100        110        120 
VEVLTGFPEV LDGRVKTLHP RVHAGLLADL RKPEHAAALE QLGIAAFELV VVNLYPFTET 

       130        140        150        160        170        180 
VDSGAGIDEC VEQIDIGGPS MVRAAAKNHP SVAVVVDPLG YDGVLAAVRH GGFTLAERKR 

       190        200        210        220        230        240 
LAALAFQHTA DYDIAVATWM ESTLAPEHPP TTFPKWLGRS WRRSAMLRYG ENPHQQASLY 

       250        260        270        280        290        300 
SDPGAWPGLA QAEQLHGKEM SYNNFTDADA AWRAAFDHEQ TCVAIIKHAN PCGIAISSIS 

       310        320        330        340        350        360 
VADAHRKAHE CDPLSAFGGV IAANTEVSVE MAEYVSTIFT EVIIAPAYQP AALEILTRKK 

       370        380        390        400        410        420 
NIRVLVASEP LTGGTELRPI SGGLLVQQRD ELDAHGDNPA NWTLATGAPA DPATLADLVF 

       430        440        450        460        470        480 
AWRVCRAVKS NAIVIAAGGA TIGVGMGQVN RVDAARLAVE RGGDRVRGAV AASDAFFPFP 

       490        500        510        520 
DGLETLTGAG VKAVVHPGGS VRDDEVTAAA ANAGITLYLT GARHFAH 

« Hide

References

« Hide 'large scale' references
[1]"Identification of the purine biosynthetic genes purN and purH in Mycobacterium avium subsp. paratuberculosis."
Urbanic K.U., Raymond M., Mutharia L.M.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 19698 / CIP 103963 / DSM 44133 / TMC 807.
[2]"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis."
Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.
Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF191543 Genomic DNA. Translation: AAF05727.1.
AE016958 Genomic DNA. Translation: AAS03220.1. Different initiation.
RefSeqNP_959837.1. NC_002944.2.

3D structure databases

ProteinModelPortalQ9RAJ5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING262316.MAP0903.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS03220; AAS03220; MAP_0903.
GeneID2718397.
KEGGmpa:MAP0903.
PATRIC17994221. VBIMycAvi108102_0944.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0138.
KOK00602.
OMADLLFAWK.
OrthoDBEOG6QCDFF.
ProtClustDBPRK00881.

Enzyme and pathway databases

BioCycMAVI262316:GCQR-920-MONOMER.
UniPathwayUPA00074; UER00133.
UPA00074; UER00135.

Family and domain databases

Gene3D3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPMF_00139. PurH.
InterProIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERPTHR11692. PTHR11692. 1 hit.
PfamPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsTIGR00355. purH. 1 hit.
ProtoNetSearch...

Entry information

Entry namePUR9_MYCPA
AccessionPrimary (citable) accession number: Q9RAJ5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 15, 2004
Last modified: February 19, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways