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Protein

Bifunctional purine biosynthesis protein PurH

Gene

purH

Organism
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. IMP cyclohydrolase activity Source: UniProtKB-HAMAP
  2. phosphoribosylaminoimidazolecarboxamide formyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transferase

Keywords - Biological processi

Purine biosynthesis

Enzyme and pathway databases

BioCyciMAVI262316:GCQR-920-MONOMER.
UniPathwayiUPA00074; UER00133.
UPA00074; UER00135.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthesis protein PurHUniRule annotation
Including the following 2 domains:
Phosphoribosylaminoimidazolecarboxamide formyltransferaseUniRule annotation (EC:2.1.2.3UniRule annotation)
Alternative name(s):
AICAR transformylaseUniRule annotation
IMP cyclohydrolaseUniRule annotation (EC:3.5.4.10UniRule annotation)
Alternative name(s):
ATICUniRule annotation
IMP synthaseUniRule annotation
InosinicaseUniRule annotation
Gene namesi
Name:purHUniRule annotation
Ordered Locus Names:MAP_0903
OrganismiMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Taxonomic identifieri262316 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
ProteomesiUP000000580: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527Bifunctional purine biosynthesis protein PurHPRO_0000192105Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi262316.MAP0903.

Structurei

3D structure databases

ProteinModelPortaliQ9RAJ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The IMP cyclohydrolase activity resides in the N-terminal region.UniRule annotation

Sequence similaritiesi

Belongs to the PurH family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0138.
KOiK00602.
OMAiPCGVAEG.
OrthoDBiEOG6QCDFF.

Family and domain databases

Gene3Di3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPiMF_00139. PurH.
InterProiIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERiPTHR11692. PTHR11692. 1 hit.
PfamiPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsiTIGR00355. purH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9RAJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDDWRENA KRPIRRALIS VYDKTGLVEL AQGLTEAGVE IVSTGSTAKV
60 70 80 90 100
IAEKGIPVTR VEVLTGFPEV LDGRVKTLHP RVHAGLLADL RKPEHAAALE
110 120 130 140 150
QLGIAAFELV VVNLYPFTET VDSGAGIDEC VEQIDIGGPS MVRAAAKNHP
160 170 180 190 200
SVAVVVDPLG YDGVLAAVRH GGFTLAERKR LAALAFQHTA DYDIAVATWM
210 220 230 240 250
ESTLAPEHPP TTFPKWLGRS WRRSAMLRYG ENPHQQASLY SDPGAWPGLA
260 270 280 290 300
QAEQLHGKEM SYNNFTDADA AWRAAFDHEQ TCVAIIKHAN PCGIAISSIS
310 320 330 340 350
VADAHRKAHE CDPLSAFGGV IAANTEVSVE MAEYVSTIFT EVIIAPAYQP
360 370 380 390 400
AALEILTRKK NIRVLVASEP LTGGTELRPI SGGLLVQQRD ELDAHGDNPA
410 420 430 440 450
NWTLATGAPA DPATLADLVF AWRVCRAVKS NAIVIAAGGA TIGVGMGQVN
460 470 480 490 500
RVDAARLAVE RGGDRVRGAV AASDAFFPFP DGLETLTGAG VKAVVHPGGS
510 520
VRDDEVTAAA ANAGITLYLT GARHFAH
Length:527
Mass (Da):55,888
Last modified:March 15, 2004 - v2
Checksum:i8B4459CFB40EC338
GO

Sequence cautioni

The sequence AAS03220.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti63 – 631V → E in AAF05727 (Ref. 1) Curated
Sequence conflicti522 – 5221A → V in AAF05727 (Ref. 1) Curated
Sequence conflicti526 – 5261A → V in AAF05727 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF191543 Genomic DNA. Translation: AAF05727.1.
AE016958 Genomic DNA. Translation: AAS03220.1. Different initiation.
RefSeqiNP_959837.1. NC_002944.2.

Genome annotation databases

EnsemblBacteriaiAAS03220; AAS03220; MAP_0903.
GeneIDi2718397.
KEGGimpa:MAP0903.
PATRICi17994221. VBIMycAvi108102_0944.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF191543 Genomic DNA. Translation: AAF05727.1.
AE016958 Genomic DNA. Translation: AAS03220.1. Different initiation.
RefSeqiNP_959837.1. NC_002944.2.

3D structure databases

ProteinModelPortaliQ9RAJ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262316.MAP0903.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS03220; AAS03220; MAP_0903.
GeneIDi2718397.
KEGGimpa:MAP0903.
PATRICi17994221. VBIMycAvi108102_0944.

Phylogenomic databases

eggNOGiCOG0138.
KOiK00602.
OMAiPCGVAEG.
OrthoDBiEOG6QCDFF.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00133.
UPA00074; UER00135.
BioCyciMAVI262316:GCQR-920-MONOMER.

Family and domain databases

Gene3Di3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPiMF_00139. PurH.
InterProiIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERiPTHR11692. PTHR11692. 1 hit.
PfamiPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsiTIGR00355. purH. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of the purine biosynthetic genes purN and purH in Mycobacterium avium subsp. paratuberculosis."
    Urbanic K.U., Raymond M., Mutharia L.M.
    Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 19698 / CIP 103963 / DSM 44133 / TMC 807.
  2. "The complete genome sequence of Mycobacterium avium subspecies paratuberculosis."
    Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.
    Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-968 / K-10.

Entry informationi

Entry nameiPUR9_MYCPA
AccessioniPrimary (citable) accession number: Q9RAJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 15, 2004
Last modified: January 7, 2015
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.