Q9RAE7 (ALR2_RHIL3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase, catabolic
EC=5.1.1.1

Gene names

Name:	dadX
Ordered Locus Names:	pRL120416

Encoded on

Plasmid pRL12 Ref.2

Organism

Rhizobium leguminosarum bv. viciae (strain 3841) [Complete proteome] [HAMAP]

Taxonomic identifier

216596 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium › Rhizobium leguminosarum › Rhizobium leguminosarum bv. viciae

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA. HAMAP-Rule MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP-Rule MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Sequence similarities	Belongs to the alanine racemase family.
Sequence caution	The sequence CAB53547.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological_process	D-alanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Alanine racemase, catabolic HAMAP-Rule MF_01201		PRO_0000114554
Sites
	Active site	51	1	Proton acceptor; specific for D-alanine By similarity
	Active site	272	1	Proton acceptor; specific for L-alanine By similarity
	Binding site	150	1	Substrate By similarity
	Binding site	320	1	Substrate; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	51	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9RAE7 [UniParc]. Last modified June 27, 2006. Version 3. Checksum: BB38DAC35BD3DBF2	FASTA	377	40,548
	10 20 30 40 50 60 MDSDILVSRT RTATIAQGAT GYLMIDLAAL GRNYRKLVSM LAPVRAGAVV KADAYGLGAE 70 80 90 100 110 120 RVARTLYSEG CRHFFVAQFV EAVRLRPALA HDAQIFVLNG LQPGNEIACA EMGIVPVLNS 130 140 150 160 170 180 LAQWRQWSAA ARILKRCLPA VLQFDTGMSR LGFPREERRE LAAALRDGSN VEILFIMSHL 190 200 210 220 230 240 ASADDMGSEQ NGEQFAEMSR IADEFPGFDI SFANSGGVFL GEAYYGVLAR PGIALYGGAP 250 260 270 280 290 300 NAGEKNPMEP VVSLNVAVVQ TRTVPAGAKV GYGGAHVTQR ETRLATIAAG YADGLPRCLS 310 320 330 340 350 360 DRGAVYFKGV RLPIVGRVSM DSTTVDITAL PEGALTFGSL VEVLGRHQTL EDIARDAGTI 370 SYEILTGLGD RYDRQYR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of alanine dehydrogenase and its role in mixed secretion of ammonium and alanine by pea bacteroids." Allaway D.A., Lodwig E.M., Crompton L.A., Wood M., Parsons R., Wheeler T.R., Poole P.S. Mol. Microbiol. 36:508-515(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome of Rhizobium leguminosarum has recognizable core and accessory components." Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F., Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H., East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I., Chillingworth T., Clarke K. , Cronin A., Davis P., Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., Whitehead S., Parkhill J. Genome Biol. 7:R34.1-R34.20(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 3841.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ249196 Genomic DNA. Translation: CAB53547.1. Different initiation. AM236086 Genomic DNA. Translation: CAK12125.1.
RefSeq	YP_764923.1. NC_008378.1.
3D structure databases
ProteinModelPortal	Q9RAE7.
ModBase	Search...
Protein-protein interaction databases
STRING	216596.pRL120416.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAK12125; CAK12125; pRL120416.
GeneID	4398234.
KEGG	rle:pRL120416.
PATRIC	23134919. VBIRhiLeg32091_0421.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HOG000031445.
KO	K01775.
OMA	KNPMEPV.
ProtClustDB	CLSK881831.
Family and domain databases
Gene3D	2.40.37.10. 1 hit.
HAMAP	MF_01201. Ala_racemase.
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR2_RHIL3

Accession

Primary (citable) accession number: Q9RAE7
Secondary accession number(s): Q1M447

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	June 27, 2006
Last modified:	May 1, 2013
This is version 81 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program