Reviewed, UniProtKB/Swiss-Prot Q9RAE6 (DADA_RHIL3)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase small subunit
    EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	pRL120417

Encoded on

Plasmid pRL12 Ref.2

Organism

Rhizobium leguminosarum bv. viciae (strain 3841) [Complete proteome] [HAMAP]

Taxonomic identifier

216596 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity.
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity.
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW potassium ion transport Inferred from electronic annotation. Source: InterPro
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP cation transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_0000166148
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9RAE6-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 335AEDA6141AC006	FASTA	416	44,838
	10 20 30 40 50 60 MKVIVLGAGI VGVTSAYQLA KAGHDVTVVD RQPGPALETS FANAGEVSFG YCSPWAAPGI 70 80 90 100 110 120 PMKAMKWLFM KHAPLILRPK LDMAMLSWMA RMLSNCTSER YAINKSRMLR LADYSRIALA 130 140 150 160 170 180 DLRAETGIAY DERMQGTLQL FRTQQQLEAS AKDVKALAAD GIPYEVLDRD GCIRFEPALK 190 200 210 220 230 240 HVRDKIVGGL LTPKDETGDC FKFTNALAAK AEALGVRFAY GTTIKALDVE AGRVRGVITD 250 260 270 280 290 300 RERMSAEAVV VALGSYSPLL LKPLGIRLPV YPVKGYSLTI PIADASRAPE STVMDETYKI 310 320 330 340 350 360 AITRLGDRIR VGGMAEISGY TNDLGLARRS TLEYSVTDLF PGGDISKASF WSGLRPMTPD 370 380 390 400 410 GTPVIGPTKV AGLFLNTGHG TLGWTMSTGS ARLIGDLVGG GQPEIDARDL AITRYG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of alanine dehydrogenase and its role in mixed secretion of ammonium and alanine by pea bacteroids." Allaway D.A., Lodwig E.M., Crompton L.A., Wood M., Parsons R., Wheeler T.R., Poole P.S. Mol. Microbiol. 36:508-515(2000) [PubMed: 10792736] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome of Rhizobium leguminosarum has recognizable core and accessory components." Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F., Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H., East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I., Chillingworth T., Clarke K. , Cronin A., Davis P., Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., Whitehead S., Parkhill J. Genome Biol. 7:RESEARCH34.1-RESEARCH34.20(2006) [PubMed: 16640791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ249196 Genomic DNA. Translation: CAB53548.1. AM236086 Genomic DNA. Translation: CAK12126.1.
RefSeq	YP_764924.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4398233.
GenomeReviews	Gene locus pRL120417 in contig AM236086_GR.
KEGG	rle:pRL120417.
NMPDR	fig|216596.1.peg.6121.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9RAE6.
OMA	Q9RAE6. MFQKHAP.
Family and domain databases
HAMAP	MF_01202. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. IPR006036. K_uptake_TrkA. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
PRINTS	PR00335. KUPTAKETRKA.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

DADA_RHIL3

Accession

Primary (citable) accession number: Q9RAE6
Secondary accession number(s): Q1M446

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)