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Protein

DNA polymerase I

Gene

polA

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

In addition to polymerase activity, this DNA polymerase 5' to 3' exonuclease activity.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  3. exonuclease activity Source: UniProtKB-KW

GO - Biological processi

  1. DNA-dependent DNA replication Source: InterPro
  2. DNA repair Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-1274-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase I (EC:2.7.7.7)
Short name:
POL I
Gene namesi
Name:polA
Ordered Locus Names:RF_1239
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 922922DNA polymerase IPRO_0000101249Add
BLAST

Interactioni

Subunit structurei

Single-chain monomer with multiple functions.By similarity

Protein-protein interaction databases

STRINGi315456.RF_1239.

Structurei

3D structure databases

ProteinModelPortaliQ9RAA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 2832835'-3' exonucleaseAdd
BLAST
Domaini439 – 48749RPE1 insertAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.Curated
Contains 1 5'-3' exonuclease domain.Curated
Contains 1 RPE1 insert domain.Curated

Phylogenomic databases

eggNOGiCOG0258.
HOGENOMiHOG000020998.
KOiK02335.
OMAiKANRPPM.
OrthoDBiEOG6SJJH7.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.50.1010. 1 hit.
InterProiIPR020046. 5-3_exonucl_a-hlix_arch_N.
IPR020045. 5-3_exonuclease_C.
IPR002421. 5-3_exonuclease_N.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR005728. Rickett_RPE.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF01367. 5_3_exonuc. 1 hit.
PF02739. 5_3_exonuc_N. 1 hit.
PF00476. DNA_pol_A. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00475. 53EXOc. 1 hit.
SM00279. HhH2. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
TIGR01045. RPE1. 1 hit.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9RAA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQKNTLLLI DGYGFVFRAY YAQQPLTSPK GEPVGALYGF ASMLLKLLSD
60 70 80 90 100
FKPKHVAVVF DSGGKNFRHH IYPEYKANRP PPPEDLVVQL PLVRDVASNL
110 120 130 140 150
NFPILEKNGY EADDIIATFA AKTAALGEDV VVISSDKDLL QLMGENIKIY
160 170 180 190 200
DPLKGKYITE DDVVKKFGTT SDKLREVMAL IGDRSDNIPG VPSIGPKTAS
210 220 230 240 250
SLITQFGSVE NIFNSLEQVS SLKQRETLQN SKEAALISWQ LIGLDSNVDL
260 270 280 290 300
DFQLNNLEWS PPNSDKLTGF LQEYGFKSLY KRAENLFDIK INDHKEIVEN
310 320 330 340 350
KVTEAKEISN ASELADFAKK AEKIGIFGIY LLQHKGDNVA LILSLQNQSY
360 370 380 390 400
IIKISNTSHD LFSYNTKNNN DWFSDIIFNL LTDKSIRKIT YSLKPLLKFY
410 420 430 440 450
AEQSHEITAI EDLELMQYAL SAGLSQKNLF EEALKEDNRH LSKPAYREEF
460 470 480 490 500
KGDTEALATA AYKSVREDAS TGSTSKLPLE TKFGKMSNVI NESARIVAEF
510 520 530 540 550
TSLYKQNILE LKDNKAFRLY SNIDLPICFI LDKMEKIGIK VDANYLNQLS
560 570 580 590 600
AEFGAEILKL EEEIFALSGT KFNIGSPKQL GEILFEKMQL PFGKASAKAS
610 620 630 640 650
SYSTGAEILE KLSEHGYNIA DLLLRWRQLT KLKNTYTDSL PKQIDNITHR
660 670 680 690 700
VHTTFLQTST TTGRLSSQEP NLQNVPIRSS EGNKIRQAFI AEEGYKLISA
710 720 730 740 750
DYSQIELRIL SHIANIDALK QAFINKDDIH TQTACQIFNL QKHELTSEHR
760 770 780 790 800
RKAKAINFGI IYGISAFGLA KQLNVSNGEA SEYIKKYFAE YKGVQEYMEQ
810 820 830 840 850
TKAFASSNGY VINFFGRKCF VPLIHDKKLK QFAERAAINA PIQGTNADII
860 870 880 890 900
KIAMINLDQE IEKNNLKTRL VLQIHDELLF EVPEDEVELV TPIIKKIMEN
910 920
STNMDVPIIT EIRVGNNWME IH
Length:922
Mass (Da):104,006
Last modified:May 1, 2000 - v1
Checksum:iEA47F799536948A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238763 Genomic DNA. Translation: CAB56067.1.
CP000053 Genomic DNA. Translation: AAY62090.1.
RefSeqiWP_011271539.1. NC_007109.1.
YP_247255.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY62090; AAY62090; RF_1239.
KEGGirfe:RF_1239.
PATRICi17893358. VBIRicFel64634_1491.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238763 Genomic DNA. Translation: CAB56067.1.
CP000053 Genomic DNA. Translation: AAY62090.1.
RefSeqiWP_011271539.1. NC_007109.1.
YP_247255.1. NC_007109.1.

3D structure databases

ProteinModelPortaliQ9RAA9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315456.RF_1239.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY62090; AAY62090; RF_1239.
KEGGirfe:RF_1239.
PATRICi17893358. VBIRicFel64634_1491.

Phylogenomic databases

eggNOGiCOG0258.
HOGENOMiHOG000020998.
KOiK02335.
OMAiKANRPPM.
OrthoDBiEOG6SJJH7.

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-1274-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.50.1010. 1 hit.
InterProiIPR020046. 5-3_exonucl_a-hlix_arch_N.
IPR020045. 5-3_exonuclease_C.
IPR002421. 5-3_exonuclease_N.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR005728. Rickett_RPE.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF01367. 5_3_exonuc. 1 hit.
PF02739. 5_3_exonuc_N. 1 hit.
PF00476. DNA_pol_A. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00475. 53EXOc. 1 hit.
SM00279. HhH2. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
TIGR01045. RPE1. 1 hit.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome degradation is an ongoing process in Rickettsia."
    Andersson J.O., Andersson S.G.E.
    Mol. Biol. Evol. 16:1178-1191(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.
  3. Cited for: DOMAIN RPE1.

Entry informationi

Entry nameiDPO1_RICFE
AccessioniPrimary (citable) accession number: Q9RAA9
Secondary accession number(s): Q4UK45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: May 1, 2000
Last modified: April 1, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.