Q9RAA9 (DPO1_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase I
Short name=POL I
EC=2.7.7.7

Gene names

Name:	polA
Ordered Locus Names:	RF_1239

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	922 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	In addition to polymerase activity, this DNA polymerase 5' to 3' exonuclease activity By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	Single-chain monomer with multiple functions By similarity.
Sequence similarities	Belongs to the DNA polymerase type-A family. Contains 1 5'-3' exonuclease domain. Contains 1 RPE1 insert domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	5'-3' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 922	922	DNA polymerase I		PRO_0000101249
Regions
	Domain	1 – 283	283	5'-3' exonuclease
	Domain	439 – 487	49	RPE1 insert

Sequences

	Sequence		Length	Mass (Da)
	Q9RAA9 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: EA47F799536948A0	FASTA	922	104,006
	10 20 30 40 50 60 MTQKNTLLLI DGYGFVFRAY YAQQPLTSPK GEPVGALYGF ASMLLKLLSD FKPKHVAVVF 70 80 90 100 110 120 DSGGKNFRHH IYPEYKANRP PPPEDLVVQL PLVRDVASNL NFPILEKNGY EADDIIATFA 130 140 150 160 170 180 AKTAALGEDV VVISSDKDLL QLMGENIKIY DPLKGKYITE DDVVKKFGTT SDKLREVMAL 190 200 210 220 230 240 IGDRSDNIPG VPSIGPKTAS SLITQFGSVE NIFNSLEQVS SLKQRETLQN SKEAALISWQ 250 260 270 280 290 300 LIGLDSNVDL DFQLNNLEWS PPNSDKLTGF LQEYGFKSLY KRAENLFDIK INDHKEIVEN 310 320 330 340 350 360 KVTEAKEISN ASELADFAKK AEKIGIFGIY LLQHKGDNVA LILSLQNQSY IIKISNTSHD 370 380 390 400 410 420 LFSYNTKNNN DWFSDIIFNL LTDKSIRKIT YSLKPLLKFY AEQSHEITAI EDLELMQYAL 430 440 450 460 470 480 SAGLSQKNLF EEALKEDNRH LSKPAYREEF KGDTEALATA AYKSVREDAS TGSTSKLPLE 490 500 510 520 530 540 TKFGKMSNVI NESARIVAEF TSLYKQNILE LKDNKAFRLY SNIDLPICFI LDKMEKIGIK 550 560 570 580 590 600 VDANYLNQLS AEFGAEILKL EEEIFALSGT KFNIGSPKQL GEILFEKMQL PFGKASAKAS 610 620 630 640 650 660 SYSTGAEILE KLSEHGYNIA DLLLRWRQLT KLKNTYTDSL PKQIDNITHR VHTTFLQTST 670 680 690 700 710 720 TTGRLSSQEP NLQNVPIRSS EGNKIRQAFI AEEGYKLISA DYSQIELRIL SHIANIDALK 730 740 750 760 770 780 QAFINKDDIH TQTACQIFNL QKHELTSEHR RKAKAINFGI IYGISAFGLA KQLNVSNGEA 790 800 810 820 830 840 SEYIKKYFAE YKGVQEYMEQ TKAFASSNGY VINFFGRKCF VPLIHDKKLK QFAERAAINA 850 860 870 880 890 900 PIQGTNADII KIAMINLDQE IEKNNLKTRL VLQIHDELLF EVPEDEVELV TPIIKKIMEN 910 920 STNMDVPIIT EIRVGNNWME IH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome degradation is an ongoing process in Rickettsia." Andersson J.O., Andersson S.G.E. Mol. Biol. Evol. 16:1178-1191(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.
[3]	"Selfish DNA in protein-coding genes of Rickettsia." Ogata H., Audic S., Barbe V., Artiguenave F., Fournier P.-E., Raoult D., Claverie J.-M. Science 290:347-350(2000) [PubMed] [Europe PMC] [Abstract] Cited for: DOMAIN RPE1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ238763 Genomic DNA. Translation: CAB56067.1. CP000053 Genomic DNA. Translation: AAY62090.1.
RefSeq	YP_247255.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q9RAA9.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_1239.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY62090; AAY62090; RF_1239.
GeneID	3401350.
KEGG	rfe:RF_1239.
PATRIC	17893358. VBIRicFel64634_1491.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0258.
HOGENOM	HOG000020998.
KO	K02335.
OMA	EQRRYAK.
ProtClustDB	PRK05755.
Family and domain databases
InterPro	IPR020046. 5-3_exonucl_a-hlix_arch_N. IPR020045. 5-3_exonuclease_C. IPR002421. 5-3_exonuclease_N. IPR019760. DNA-dir_DNA_pol_A_CS. IPR001098. DNA-dir_DNA_pol_A_palm_dom. IPR018320. DNA_polymerase_1. IPR002298. DNA_polymerase_A. IPR008918. HhH2. IPR005728. Rickett_RPE. IPR012337. RNaseH-like_dom. [Graphical view]
Pfam	PF01367. 5_3_exonuc. 1 hit. PF02739. 5_3_exonuc_N. 1 hit. PF00476. DNA_pol_A. 1 hit. [Graphical view]
PRINTS	PR00868. DNAPOLI.
SMART	SM00475. 53EXOc. 1 hit. SM00279. HhH2. 1 hit. SM00482. POLAc. 1 hit. [Graphical view]
SUPFAM	SSF47807. 5_3_exo_C. 1 hit. SSF53098. RNaseH_fold. 1 hit.
TIGRFAMs	TIGR00593. pola. 1 hit. TIGR01045. RPE1. 1 hit.
PROSITE	PS00447. DNA_POLYMERASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DPO1_RICFE

Accession

Primary (citable) accession number: Q9RAA9
Secondary accession number(s): Q4UK45

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program