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Q9R9N4

- ODPB_RHIME

UniProt

Q9R9N4 - ODPB_RHIME

Protein

Pyruvate dehydrogenase E1 component subunit beta

Gene

pdhB

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.By similarity

    Catalytic activityi

    Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

    Cofactori

    Binds 1 lipoyl cofactor covalently.By similarity
    Binds 1 thiamine pyrophosphate per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei194 – 1941Thiamine pyrophosphateBy similarity

    GO - Molecular functioni

    1. pyruvate dehydrogenase (acetyl-transferring) activity Source: UniProtKB-EC

    GO - Biological processi

    1. acetyl-CoA biosynthetic process from pyruvate Source: InterPro
    2. glycolytic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Pyruvate, Thiamine pyrophosphate

    Enzyme and pathway databases

    BioCyciSMEL266834:GJF6-1481-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate dehydrogenase E1 component subunit beta (EC:1.2.4.1)
    Gene namesi
    Name:pdhB
    Synonyms:pdhAbeta
    Ordered Locus Names:R01446
    ORF Names:SMc01031
    OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
    Taxonomic identifieri266834 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001976: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 460460Pyruvate dehydrogenase E1 component subunit betaPRO_0000162225Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei43 – 431N6-lipoyllysineSequence Analysis

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and a beta chain.

    Protein-protein interaction databases

    STRINGi266834.SMc01031.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R9N4.
    SMRiQ9R9N4. Positions 137-457.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 7777Lipoyl-bindingAdd
    BLAST

    Sequence similaritiesi

    Contains 1 lipoyl-binding domain.Curated

    Keywords - Domaini

    Lipoyl

    Phylogenomic databases

    eggNOGiCOG0508.
    HOGENOMiHOG000281450.
    KOiK00162.
    OMAiEISTHSF.
    OrthoDBiEOG6JQH4C.

    Family and domain databases

    Gene3Di3.40.50.920. 1 hit.
    3.40.50.970. 1 hit.
    InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
    IPR000089. Biotin_lipoyl.
    IPR027110. PDHB.
    IPR011053. Single_hybrid_motif.
    IPR029061. THDP-binding.
    IPR009014. Transketo_C/Pyr-ferredox_oxred.
    IPR005475. Transketolase-like_Pyr-bd.
    IPR005476. Transketolase_C.
    [Graphical view]
    PANTHERiPTHR11624:SF56. PTHR11624:SF56. 1 hit.
    PfamiPF00364. Biotin_lipoyl. 1 hit.
    PF02779. Transket_pyr. 1 hit.
    PF02780. Transketolase_C. 1 hit.
    [Graphical view]
    SMARTiSM00861. Transket_pyr. 1 hit.
    [Graphical view]
    SUPFAMiSSF51230. SSF51230. 1 hit.
    SSF52518. SSF52518. 1 hit.
    SSF52922. SSF52922. 1 hit.
    PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
    PS00189. LIPOYL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9R9N4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPVEILMPAL SPTMEEGTLS KWLKNEGDKV SSGDVIAEIE TDKATMEVEA    50
    VDEGTIGKLL IAAGTEGVKV NTPIAVLLQD GEAASDIDSM KTEAPKAETP 100
    KPAAAEAPAA SAAPVAAQPK ADVPSDPAIP AGTEMATMTV REALRDAMAE 150
    EMRANEDVFV MGEEVAEYQG AYKVTQGLLQ EFGARRVVDT PITEHGFAGV 200
    GVGAAMTGLR PIVEFMTFNF AMQAIDQIIN SAAKTLYMSG GQMGAPIVFR 250
    GPSGAAARVA AQHSQCYAAW YSHIPGLKVV MPYTAADAKG LLKAAIRDPN 300
    PVIFLENEIL YGQSFEVPKL DDFVLPIGKA RIHRTGKDAT LVSFGIGMTY 350
    AIKAAAELEA QGIDVEIIDL RTIRPMDLPT VIESVKKTGR LVTVEEGYPQ 400
    SSVGTEIATR VMQQAFDYLD APILTIAGKD VPMPYAANLE KLALPNVAEV 450
    VDAVKAVCYK 460
    Length:460
    Mass (Da):48,812
    Last modified:May 30, 2000 - v2
    Checksum:i9FA3E5A30EDC3C6F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti53 – 531E → K in AAF04588. (PubMed:10796014)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF190792 Genomic DNA. Translation: AAF04588.1.
    AL591688 Genomic DNA. Translation: CAC46025.1.
    RefSeqiNP_385552.1. NC_003047.1.

    Genome annotation databases

    EnsemblBacteriaiCAC46025; CAC46025; SMc01031.
    GeneIDi1233099.
    KEGGisme:SMc01031.
    PATRICi23632229. VBISinMel96828_2866.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF190792 Genomic DNA. Translation: AAF04588.1 .
    AL591688 Genomic DNA. Translation: CAC46025.1 .
    RefSeqi NP_385552.1. NC_003047.1.

    3D structure databases

    ProteinModelPortali Q9R9N4.
    SMRi Q9R9N4. Positions 137-457.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 266834.SMc01031.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC46025 ; CAC46025 ; SMc01031 .
    GeneIDi 1233099.
    KEGGi sme:SMc01031.
    PATRICi 23632229. VBISinMel96828_2866.

    Phylogenomic databases

    eggNOGi COG0508.
    HOGENOMi HOG000281450.
    KOi K00162.
    OMAi EISTHSF.
    OrthoDBi EOG6JQH4C.

    Enzyme and pathway databases

    BioCyci SMEL266834:GJF6-1481-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.920. 1 hit.
    3.40.50.970. 1 hit.
    InterProi IPR003016. 2-oxoA_DH_lipoyl-BS.
    IPR000089. Biotin_lipoyl.
    IPR027110. PDHB.
    IPR011053. Single_hybrid_motif.
    IPR029061. THDP-binding.
    IPR009014. Transketo_C/Pyr-ferredox_oxred.
    IPR005475. Transketolase-like_Pyr-bd.
    IPR005476. Transketolase_C.
    [Graphical view ]
    PANTHERi PTHR11624:SF56. PTHR11624:SF56. 1 hit.
    Pfami PF00364. Biotin_lipoyl. 1 hit.
    PF02779. Transket_pyr. 1 hit.
    PF02780. Transketolase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00861. Transket_pyr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51230. SSF51230. 1 hit.
    SSF52518. SSF52518. 1 hit.
    SSF52922. SSF52922. 1 hit.
    PROSITEi PS50968. BIOTINYL_LIPOYL. 1 hit.
    PS00189. LIPOYL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Symbiotic induction of pyruvate dehydrogenase genes from Sinorhizobium meliloti."
      Cabanes D., Boistard P., Batut J.
      Mol. Plant Microbe Interact. 13:483-493(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: RCR2011 / SU47.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.

    Entry informationi

    Entry nameiODPB_RHIME
    AccessioniPrimary (citable) accession number: Q9R9N4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3