Q9R9N4 (ODPB_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate dehydrogenase E1 component subunit beta
EC=1.2.4.1

Gene names

Name:	pdhB
Synonyms:	pdhAbeta
Ordered Locus Names:	R01446
ORF Names:	SMc01031

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Rhizobium meliloti

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.
Catalytic activity	Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.
Cofactor	Binds 1 lipoyl cofactor covalently By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Subunit structure	Heterodimer of an alpha and a beta chain.
Sequence similarities	Contains 1 lipoyl-binding domain.

Ontologies

Keywords
Biological process	Glycolysis
Domain	Lipoyl
Ligand	Pyruvate Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	acetyl-CoA biosynthetic process from pyruvate Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	pyruvate dehydrogenase (acetyl-transferring) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	Pyruvate dehydrogenase E1 component subunit beta		PRO_0000162225
Regions
	Domain	1 – 77	77	Lipoyl-binding
Sites
	Binding site	194	1	Thiamine pyrophosphate By similarity
Amino acid modifications
	Modified residue	43	1	N6-lipoyllysine Potential
Experimental info
	Sequence conflict	53	1	E → K in AAF04588. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9R9N4 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 9FA3E5A30EDC3C6F	FASTA	460	48,812
	10 20 30 40 50 60 MPVEILMPAL SPTMEEGTLS KWLKNEGDKV SSGDVIAEIE TDKATMEVEA VDEGTIGKLL 70 80 90 100 110 120 IAAGTEGVKV NTPIAVLLQD GEAASDIDSM KTEAPKAETP KPAAAEAPAA SAAPVAAQPK 130 140 150 160 170 180 ADVPSDPAIP AGTEMATMTV REALRDAMAE EMRANEDVFV MGEEVAEYQG AYKVTQGLLQ 190 200 210 220 230 240 EFGARRVVDT PITEHGFAGV GVGAAMTGLR PIVEFMTFNF AMQAIDQIIN SAAKTLYMSG 250 260 270 280 290 300 GQMGAPIVFR GPSGAAARVA AQHSQCYAAW YSHIPGLKVV MPYTAADAKG LLKAAIRDPN 310 320 330 340 350 360 PVIFLENEIL YGQSFEVPKL DDFVLPIGKA RIHRTGKDAT LVSFGIGMTY AIKAAAELEA 370 380 390 400 410 420 QGIDVEIIDL RTIRPMDLPT VIESVKKTGR LVTVEEGYPQ SSVGTEIATR VMQQAFDYLD 430 440 450 460 APILTIAGKD VPMPYAANLE KLALPNVAEV VDAVKAVCYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Symbiotic induction of pyruvate dehydrogenase genes from Sinorhizobium meliloti." Cabanes D., Boistard P., Batut J. Mol. Plant Microbe Interact. 13:483-493(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RCR2011 / SU47.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[3]	"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF190792 Genomic DNA. Translation: AAF04588.1. AL591688 Genomic DNA. Translation: CAC46025.1.
RefSeq	NP_385552.1. NC_003047.1.
3D structure databases
ProteinModelPortal	Q9R9N4.
SMR	Q9R9N4. Positions 137-457.
ModBase	Search...
Protein-protein interaction databases
STRING	266834.SMc01031.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC46025; CAC46025; SMc01031.
GeneID	1233099.
KEGG	sme:SMc01031.
PATRIC	23632229. VBISinMel96828_2866.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0508.
HOGENOM	HOG000281450.
KO	K00162.
OMA	QHSQDYS.
ProtClustDB	PRK11892.
Enzyme and pathway databases
BioCyc	SMEL266834:GJF6-1481-MONOMER.
Family and domain databases
Gene3D	3.40.50.920. 1 hit.
InterPro	IPR003016. 2-oxoA_DH_lipoyl-BS. IPR000089. Biotin_lipoyl. IPR027110. PDHB. IPR011053. Single_hybrid_motif. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. [Graphical view]
PANTHER	PTHR11624:SF11. PTHR11624:SF11. 1 hit.
Pfam	PF00364. Biotin_lipoyl. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF51230. Hybrid_motif. 1 hit. SSF52922. Transketo_C_like. 1 hit.
PROSITE	PS50968. BIOTINYL_LIPOYL. 1 hit. PS00189. LIPOYL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ODPB_RHIME

Accession

Primary (citable) accession number: Q9R9N4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program