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Q9R9J2

- FENF_BACIU

UniProt

Q9R9J2 - FENF_BACIU

Protein

Malonyl CoA-acyl carrier protein transacylase

Gene

fenF

Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Is involved in the mycosubtilin synthetase assembly, by catalyzing the transfer of malonyl groups to a specific acyl-carrier-protein domain on MycA.1 Publication

    Catalytic activityi

    Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein].1 Publication

    Kineticsi

    1. KM=45 µM for malonyl-CoA1 Publication
    2. KM=950 µM for acyl-carrier-protein domain of MycA1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei92 – 921By similarity
    Active sitei201 – 2011By similarity

    GO - Molecular functioni

    1. [acyl-carrier-protein] S-malonyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fatty acid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malonyl CoA-acyl carrier protein transacylase (EC:2.3.1.39)
    Short name:
    MCT
    Gene namesi
    Name:fenF
    OrganismiBacillus subtilis
    Taxonomic identifieri1423 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 400400Malonyl CoA-acyl carrier protein transacylasePRO_0000385454Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R9J2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FabD family.Curated

    Family and domain databases

    Gene3Di3.40.366.10. 2 hits.
    InterProiIPR001227. Ac_transferase_dom.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR004410. Malonyl_CoA-ACP_transAc_FabD.
    IPR016036. Malonyl_transacylase_ACP-bd.
    [Graphical view]
    PfamiPF00698. Acyl_transf_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF52151. SSF52151. 2 hits.
    SSF55048. SSF55048. 1 hit.
    TIGRFAMsiTIGR00128. fabD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9R9J2-1 [UniParc]FASTAAdd to Basket

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    MNNLAFLFPG QGSQFVGMGK SFWNDFVLAK RLFEEASDAI SMDVKKLCFD    50
    GDMTELTRTM NAQPAILTVS VIAYQVYMQE IGIKPHFLAG HSLGEYSALV 100
    CAGVLSFQEA VKLIRQRGIL MQNADPEQLG TMAAITQVYI QPLQDLCTEI 150
    STEDFPVGVA CMNSDQQHVI SGHRQAVEFV IKKAERMGAN HTYLNVSAPF 200
    HSSMMRSASE QFQTALNQYS FRDAEWPIIS NVTAIPYNNG HSVREHLQTH 250
    MTMPVRWAES MHYLLLHGVT EVIEMGPKNV LVGLLKKITN HIAAYPLGQT 300
    SDLHLLSDSA ERNENIVNLR KKQLNKMMIQ SIIARNYNKD AKTYSNLTTP 350
    LFPQIQLLKE RVERKEVELS AEELEHSIHL CQLICEAKQL PTWEQLRILK 400
    Length:400
    Mass (Da):45,222
    Last modified:May 1, 2000 - v1
    Checksum:i2A4430664C90C578
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF184956 Genomic DNA. Translation: AAF08794.1.
    PIRiT44805.

    Genome annotation databases

    PATRICi37933545. VBIBacSub155824_1076.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF184956 Genomic DNA. Translation: AAF08794.1 .
    PIRi T44805.

    3D structure databases

    ProteinModelPortali Q9R9J2.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    PATRICi 37933545. VBIBacSub155824_1076.

    Family and domain databases

    Gene3Di 3.40.366.10. 2 hits.
    InterProi IPR001227. Ac_transferase_dom.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR004410. Malonyl_CoA-ACP_transAc_FabD.
    IPR016036. Malonyl_transacylase_ACP-bd.
    [Graphical view ]
    Pfami PF00698. Acyl_transf_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52151. SSF52151. 2 hits.
    SSF55048. SSF55048. 1 hit.
    TIGRFAMsi TIGR00128. fabD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The mycosubtilin synthetase of Bacillus subtilis ATCC6633: a multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase."
      Duitman E.H., Hamoen L.W., Rembold M., Venema G., Seitz H., Saenger W., Bernhard F., Reinhardt R., Schmidt M., Ullrich C., Stein T., Leenders F., Vater J.
      Proc. Natl. Acad. Sci. U.S.A. 96:13294-13299(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 6633 / DSM 347 / IFO 3134 / PCI 219 / NRS 231.
    2. "FenF: servicing the mycosubtilin synthetase assembly line in trans."
      Aron Z.D., Fortin P.D., Calderone C.T., Walsh C.T.
      ChemBioChem 8:613-616(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: ATCC 6633 / DSM 347 / IFO 3134 / PCI 219 / NRS 231.

    Entry informationi

    Entry nameiFENF_BACIU
    AccessioniPrimary (citable) accession number: Q9R9J2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3