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Q9R9J2 (FENF_BACIU) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malonyl CoA-acyl carrier protein transacylase

Short name=MCT
EC=2.3.1.39
Gene names
Name:fenF
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Is involved in the mycosubtilin synthetase assembly, by catalyzing the transfer of malonyl groups to a specific acyl-carrier-protein domain on MycA. Ref.2

Catalytic activity

Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein]. Ref.2

Sequence similarities

Belongs to the FabD family.

Biophysicochemical properties

Kinetic parameters:

KM=45 µM for malonyl-CoA Ref.2

KM=950 µM for acyl-carrier-protein domain of MycA

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 400400Malonyl CoA-acyl carrier protein transacylase
PRO_0000385454

Sites

Active site921 By similarity
Active site2011 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9R9J2 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 2A4430664C90C578

FASTA40045,222
        10         20         30         40         50         60 
MNNLAFLFPG QGSQFVGMGK SFWNDFVLAK RLFEEASDAI SMDVKKLCFD GDMTELTRTM 

        70         80         90        100        110        120 
NAQPAILTVS VIAYQVYMQE IGIKPHFLAG HSLGEYSALV CAGVLSFQEA VKLIRQRGIL 

       130        140        150        160        170        180 
MQNADPEQLG TMAAITQVYI QPLQDLCTEI STEDFPVGVA CMNSDQQHVI SGHRQAVEFV 

       190        200        210        220        230        240 
IKKAERMGAN HTYLNVSAPF HSSMMRSASE QFQTALNQYS FRDAEWPIIS NVTAIPYNNG 

       250        260        270        280        290        300 
HSVREHLQTH MTMPVRWAES MHYLLLHGVT EVIEMGPKNV LVGLLKKITN HIAAYPLGQT 

       310        320        330        340        350        360 
SDLHLLSDSA ERNENIVNLR KKQLNKMMIQ SIIARNYNKD AKTYSNLTTP LFPQIQLLKE 

       370        380        390        400 
RVERKEVELS AEELEHSIHL CQLICEAKQL PTWEQLRILK 

« Hide

References

[1]"The mycosubtilin synthetase of Bacillus subtilis ATCC6633: a multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase."
Duitman E.H., Hamoen L.W., Rembold M., Venema G., Seitz H., Saenger W., Bernhard F., Reinhardt R., Schmidt M., Ullrich C., Stein T., Leenders F., Vater J.
Proc. Natl. Acad. Sci. U.S.A. 96:13294-13299(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 6633 / DSM 347 / IFO 3134 / PCI 219 / NRS 231.
[2]"FenF: servicing the mycosubtilin synthetase assembly line in trans."
Aron Z.D., Fortin P.D., Calderone C.T., Walsh C.T.
ChemBioChem 8:613-616(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: ATCC 6633 / DSM 347 / IFO 3134 / PCI 219 / NRS 231.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF184956 Genomic DNA. Translation: AAF08794.1.
PIRT44805.

3D structure databases

ProteinModelPortalQ9R9J2.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

PATRIC37933545. VBIBacSub155824_1076.

Family and domain databases

Gene3D3.40.366.10. 2 hits.
InterProIPR001227. Ac_transferase_dom.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR004410. Malonyl_CoA-ACP_transAc_FabD.
IPR016036. Malonyl_transacylase_ACP-bd.
[Graphical view]
PfamPF00698. Acyl_transf_1. 1 hit.
[Graphical view]
SUPFAMSSF52151. SSF52151. 2 hits.
SSF55048. SSF55048. 1 hit.
TIGRFAMsTIGR00128. fabD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameFENF_BACIU
AccessionPrimary (citable) accession number: Q9R9J2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 1, 2000
Last modified: October 16, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families