Q9R9J2 (FENF_BACIU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malonyl CoA-acyl carrier protein transacylase
Short name=MCT
EC=2.3.1.39

Gene names

Name:

fenF

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Is involved in the mycosubtilin synthetase assembly, by catalyzing the transfer of malonyl groups to a specific acyl-carrier-protein domain on MycA. Ref.2
Catalytic activity	Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein]. Ref.2
Sequence similarities	Belongs to the FabD family.
Biophysicochemical properties	Kinetic parameters: KM=45 µM for malonyl-CoA Ref.2 KM=950 µM for acyl-carrier-protein domain of MycA

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	[acyl-carrier-protein] S-malonyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Malonyl CoA-acyl carrier protein transacylase		PRO_0000385454
Sites
	Active site	92	1	By similarity
	Active site	201	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9R9J2 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 2A4430664C90C578	FASTA	400	45,222
	10 20 30 40 50 60 MNNLAFLFPG QGSQFVGMGK SFWNDFVLAK RLFEEASDAI SMDVKKLCFD GDMTELTRTM 70 80 90 100 110 120 NAQPAILTVS VIAYQVYMQE IGIKPHFLAG HSLGEYSALV CAGVLSFQEA VKLIRQRGIL 130 140 150 160 170 180 MQNADPEQLG TMAAITQVYI QPLQDLCTEI STEDFPVGVA CMNSDQQHVI SGHRQAVEFV 190 200 210 220 230 240 IKKAERMGAN HTYLNVSAPF HSSMMRSASE QFQTALNQYS FRDAEWPIIS NVTAIPYNNG 250 260 270 280 290 300 HSVREHLQTH MTMPVRWAES MHYLLLHGVT EVIEMGPKNV LVGLLKKITN HIAAYPLGQT 310 320 330 340 350 360 SDLHLLSDSA ERNENIVNLR KKQLNKMMIQ SIIARNYNKD AKTYSNLTTP LFPQIQLLKE 370 380 390 400 RVERKEVELS AEELEHSIHL CQLICEAKQL PTWEQLRILK

References

[1]

"The mycosubtilin synthetase of Bacillus subtilis ATCC6633: a multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase." Duitman E.H., Hamoen L.W., Rembold M., Venema G., Seitz H., Saenger W., Bernhard F., Reinhardt R., Schmidt M., Ullrich C., Stein T., Leenders F., Vater J. Proc. Natl. Acad. Sci. U.S.A. 96:13294-13299(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6633 / DSM 347 / IFO 3134 / PCI 219 / NRS 231.

[2]

"FenF: servicing the mycosubtilin synthetase assembly line in trans." Aron Z.D., Fortin P.D., Calderone C.T., Walsh C.T. ChemBioChem 8:613-616(2007) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: ATCC 6633 / DSM 347 / IFO 3134 / PCI 219 / NRS 231.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF184956 Genomic DNA. Translation: AAF08794.1.
PIR	T44805.
3D structure databases
ProteinModelPortal	Q9R9J2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
PATRIC	37933545. VBIBacSub155824_1076.
Family and domain databases
Gene3D	3.40.366.10. 2 hits.
InterPro	IPR001227. Ac_transferase_dom. IPR014043. Acyl_transferase. IPR016035. Acyl_Trfase/lysoPLipase. IPR004410. Malonyl_CoA-ACP_transAc_FabD. IPR016036. Malonyl_transacylase_ACP-bd. [Graphical view]
Pfam	PF00698. Acyl_transf_1. 1 hit. [Graphical view]
SUPFAM	SSF52151. Acyl_Trfase/lysoPlipase. 1 hit. SSF55048. Malonyl_transacylase_ACP-bd. 1 hit.
TIGRFAMs	TIGR00128. fabD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FENF_BACIU

Accession

Primary (citable) accession number: Q9R9J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	May 1, 2000
Last modified:	May 29, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program