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Protein

Malonyl CoA-acyl carrier protein transacylase

Gene

fenF

Organism
Bacillus subtilis
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Is involved in the mycosubtilin synthetase assembly, by catalyzing the transfer of malonyl groups to a specific acyl-carrier-protein domain on MycA.1 Publication

Catalytic activityi

Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein].1 Publication

Kineticsi

  1. KM=45 µM for malonyl-CoA1 Publication
  2. KM=950 µM for acyl-carrier-protein domain of MycA1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei92 – 921By similarity
    Active sitei201 – 2011By similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malonyl CoA-acyl carrier protein transacylase (EC:2.3.1.39)
    Short name:
    MCT
    Gene namesi
    Name:fenF
    OrganismiBacillus subtilis
    Taxonomic identifieri1423 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 400400Malonyl CoA-acyl carrier protein transacylasePRO_0000385454Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R9J2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FabD family.Curated

    Family and domain databases

    Gene3Di3.40.366.10. 2 hits.
    InterProiIPR001227. Ac_transferase_dom.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR004410. Malonyl_CoA-ACP_transAc_FabD.
    IPR016036. Malonyl_transacylase_ACP-bd.
    [Graphical view]
    PfamiPF00698. Acyl_transf_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF52151. SSF52151. 2 hits.
    SSF55048. SSF55048. 1 hit.
    TIGRFAMsiTIGR00128. fabD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9R9J2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNNLAFLFPG QGSQFVGMGK SFWNDFVLAK RLFEEASDAI SMDVKKLCFD
    60 70 80 90 100
    GDMTELTRTM NAQPAILTVS VIAYQVYMQE IGIKPHFLAG HSLGEYSALV
    110 120 130 140 150
    CAGVLSFQEA VKLIRQRGIL MQNADPEQLG TMAAITQVYI QPLQDLCTEI
    160 170 180 190 200
    STEDFPVGVA CMNSDQQHVI SGHRQAVEFV IKKAERMGAN HTYLNVSAPF
    210 220 230 240 250
    HSSMMRSASE QFQTALNQYS FRDAEWPIIS NVTAIPYNNG HSVREHLQTH
    260 270 280 290 300
    MTMPVRWAES MHYLLLHGVT EVIEMGPKNV LVGLLKKITN HIAAYPLGQT
    310 320 330 340 350
    SDLHLLSDSA ERNENIVNLR KKQLNKMMIQ SIIARNYNKD AKTYSNLTTP
    360 370 380 390 400
    LFPQIQLLKE RVERKEVELS AEELEHSIHL CQLICEAKQL PTWEQLRILK
    Length:400
    Mass (Da):45,222
    Last modified:May 1, 2000 - v1
    Checksum:i2A4430664C90C578
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF184956 Genomic DNA. Translation: AAF08794.1.
    PIRiT44805.
    RefSeqiWP_003220190.1. NZ_KN049967.1.

    Genome annotation databases

    PATRICi37933545. VBIBacSub155824_1076.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF184956 Genomic DNA. Translation: AAF08794.1.
    PIRiT44805.
    RefSeqiWP_003220190.1. NZ_KN049967.1.

    3D structure databases

    ProteinModelPortaliQ9R9J2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    PATRICi37933545. VBIBacSub155824_1076.

    Family and domain databases

    Gene3Di3.40.366.10. 2 hits.
    InterProiIPR001227. Ac_transferase_dom.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR004410. Malonyl_CoA-ACP_transAc_FabD.
    IPR016036. Malonyl_transacylase_ACP-bd.
    [Graphical view]
    PfamiPF00698. Acyl_transf_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF52151. SSF52151. 2 hits.
    SSF55048. SSF55048. 1 hit.
    TIGRFAMsiTIGR00128. fabD. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    1. "The mycosubtilin synthetase of Bacillus subtilis ATCC6633: a multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase."
      Duitman E.H., Hamoen L.W., Rembold M., Venema G., Seitz H., Saenger W., Bernhard F., Reinhardt R., Schmidt M., Ullrich C., Stein T., Leenders F., Vater J.
      Proc. Natl. Acad. Sci. U.S.A. 96:13294-13299(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 6633 / DSM 347 / IFO 3134 / PCI 219 / NRS 231.
    2. "FenF: servicing the mycosubtilin synthetase assembly line in trans."
      Aron Z.D., Fortin P.D., Calderone C.T., Walsh C.T.
      ChemBioChem 8:613-616(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: ATCC 6633 / DSM 347 / IFO 3134 / PCI 219 / NRS 231.

    Entry informationi

    Entry nameiFENF_BACIU
    AccessioniPrimary (citable) accession number: Q9R9J2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: May 1, 2000
    Last modified: January 7, 2015
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.