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Q9R9J1

- MYCA_BACIU

UniProt

Q9R9J1 - MYCA_BACIU

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Protein

Mycosubtilin synthase subunit A

Gene

mycA

Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This protein is a multifunctional enzyme, able to activate a long chain fatty acid and link it with the amino acid Asn as part of the synthesis of mycosubtilin. The activation sites consist of individual domains.1 Publication

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei843 – 8431For beta-ketoacyl synthase activityPROSITE-ProRule annotation

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. phosphopantetheine binding Source: InterPro
  3. pyridoxal phosphate binding Source: InterPro
  4. transaminase activity Source: InterPro

GO - Biological processi

  1. antibiotic biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Antibiotic biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Mycosubtilin synthase subunit A (EC:2.3.1.-)
Including the following 2 domains:
Glutamate-1-semialdehyde aminotransferase
Short name:
GSA-AT
ATP-dependent asparagine adenylase 1
Short name:
AsnA 1
Alternative name(s):
Asparagine activase 1
Gene namesi
Name:mycA
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 39713971Mycosubtilin synthase subunit APRO_0000360848Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei613 – 6131O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation
Modified residuei1324 – 13241O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation
Modified residuei1759 – 17591N6-(pyridoxal phosphate)lysineBy similarity
Modified residuei2440 – 24401O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation
Modified residuei3477 – 34771O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliQ9R9J1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini583 – 65068Acyl carrier 1PROSITE-ProRule annotationAdd
BLAST
Domaini1292 – 136170Acyl carrier 2PROSITE-ProRule annotationAdd
BLAST
Domaini2410 – 247768Acyl carrier 3PROSITE-ProRule annotationAdd
BLAST
Domaini3447 – 351468Acyl carrier 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni160 – 479320Acyl-CoA ligaseAdd
BLAST
Regioni672 – 1095424Beta-ketoacyl synthaseAdd
BLAST
Regioni1529 – 1856328GSA-ATAdd
BLAST
Regioni1938 – 2240303Condensation 1Add
BLAST
Regioni2492 – 2781290Condensation 2Add
BLAST
Regioni2937 – 3823887Domain 1 (asparagine-activating)Add
BLAST
Regioni2967 – 3364398Adenylation 1Add
BLAST
Regioni3529 – 3818290Condensation 3Add
BLAST

Sequence similaritiesi

Contains 4 acyl carrier domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.10.1200.10. 4 hits.
3.40.47.10. 2 hits.
3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProiIPR010071. AA_adenyl_domain.
IPR009081. Acyl_carrier_prot-like.
IPR005814. Aminotrans_3.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR001242. Condensatn.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR020806. PKS_PP-bd.
IPR006162. PPantetheine_attach_site.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00202. Aminotran_3. 1 hit.
PF00501. AMP-binding. 2 hits.
PF13193. AMP-binding_C. 1 hit.
PF00668. Condensation. 3 hits.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF00550. PP-binding. 4 hits.
[Graphical view]
SMARTiSM00823. PKS_PP. 4 hits.
[Graphical view]
SUPFAMiSSF47336. SSF47336. 4 hits.
SSF53383. SSF53383. 1 hit.
SSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01733. AA-adenyl-dom. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
PS50075. ACP_DOMAIN. 4 hits.
PS00455. AMP_BINDING. 2 hits.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R9J1-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MYTSQFQTLV DVIRNRSNIS DRGIRFIESD KIETFVSYRQ LFDEAQGFLG
60 70 80 90 100
YLQHIGIQPK QEIVFQIQEN KSFVVAFWAC LLGGMIPVPV SIGEDNDHKL
110 120 130 140 150
KVWRIWNILN NPFLLASETV LDKMKKFAAD HDLQDFHHQL IEKSDIIQDR
160 170 180 190 200
IYDHPASQYE PEADELAFIQ FSSGSTGDPK GVMLTHHNLI HNTCAIRNAL
210 220 230 240 250
AIDLKDTLLS WMPLTHDMGL IACHLVPALA GINQNLMPTE LFIRRPILWM
260 270 280 290 300
KKAHEHKASI LSSPNFGYNY FLKFLKDNKS YDWDLSHIRV IANGAEPILP
310 320 330 340 350
ELCDEFLTRC AAFNMKRSAI LNVYGLAEAS VGATFSNIGE RFVPVYLHRD
360 370 380 390 400
HLNLGERAVE VSKEDQNCAS FVEVGKPIDY CQIRICNEAN EGLEDGFIGH
410 420 430 440 450
IQIKGENVTQ GYYNNPESTN RALTPDGWVK TGDLGFIRKG NLVVTGREKD
460 470 480 490 500
IIFVNGKNVY PHDIERVAIE LEDIDLGRVA ACGVYDQETR SREIVLFAVY
510 520 530 540 550
KKSADRFAPL VKDIKKHLYQ RGGWSIKEIL PIRKLPKTTS GKVKRYELAE
560 570 580 590 600
QYESGKFALE STKIKEFLEG HSTEPVQTPI HEIETALLSI FSEVMDGKKI
610 620 630 640 650
HLNDHYFDMG ATSLQLSQIA ERIEQKFGCE LTVADLFTYP SIADLAAFLV
660 670 680 690 700
ENHSEIKQTD TAKPSRSSSK DIAIIGMSLN VPGASNKSDF WHLLENGEHG
710 720 730 740 750
IREYPAPRVK DAIDYLRSIK SERNEKQFVR GGYLDEIDRF DYSFFGLAPK
760 770 780 790 800
TAKFMDPNQR LFLQSAWHAI EDAGYAGDTI SGSQLGVYVG YSKVGYDYER
810 820 830 840 850
LLSANYPEEL HHYIVGNLPS VLASRIAYFL NLKGPAVTVD TACSSSLVAV
860 870 880 890 900
HMACKALLTG DCEMALAGGI RTSLLPMRIG LDMESSDGLT KTFSKDSDGT
910 920 930 940 950
GSGEGVAAVL LKPLQAAIRD GDHIYGVIKG SAINQDGTTV GITAPSPAAQ
960 970 980 990 1000
TEVIEMAWKD AGIAPETLSF IEAHGTGTKL GDPVEFNGLC KAFEKVTEKK
1010 1020 1030 1040 1050
QFCAIGSVKA NIGHLFEAAG IVGLIKSALM LNHKKIPPLA HFNKPNPLIP
1060 1070 1080 1090 1100
FHSSPFYVNQ EVMDFTPEDR PLRGGISSFG FSGTNAHVVL EEYTPESEYA
1110 1120 1130 1140 1150
PEDGNDPHLF VLSAHTEASL YELTHQYRQY ISDDSQSSLR SICYTASTGR
1160 1170 1180 1190 1200
AHLDYCLAMI VSSNQELIDK LTSLIQGERN LPQVHFGYKN IKEMQPAEKD
1210 1220 1230 1240 1250
NLSKQISDLM QHRPCTKDER ITWLNRIAEL YVQRAVIDWR AVYSNEVVQK
1260 1270 1280 1290 1300
TPLPLYPFER NRCWVEAVYE SAKERKEKGE VALDINHTKT HIESFLKTVI
1310 1320 1330 1340 1350
SNASGIRADE IDSNAHFIGF GLDSIMLTQV KKAIADEFNV DIPMERFFDT
1360 1370 1380 1390 1400
MNNIESVVDY LAENVPSAAS TPPQESVTAQ EELVISGAQP ELEHQEHMLD
1410 1420 1430 1440 1450
KIIASQNQLI QQTLQAQLDS FNLLRNNSHF VSKESEISQD KTSLSPKSVT
1460 1470 1480 1490 1500
AKKNSAQEAK PYIPFQRQTL NEQVNYTPQQ RQYLESFIEK YVDKTKGSKQ
1510 1520 1530 1540 1550
YTDETRFAHA NNRNLSSFRS YWKEMVYPII AERSDGSRMW DIDGNEYIDI
1560 1570 1580 1590 1600
TMGFGVNLFG HHPSFITQTV VDSTHSALPP LGPMSNVAGE VADRIRACTG
1610 1620 1630 1640 1650
VERVAFYNSG TEAVMVALRL ARAATGRTKV VVFAGSYHGT FDGVLGVANT
1660 1670 1680 1690 1700
KGGAEPANPL APGIPQSFMN DLIILHYNHP DSLDVIRNLG NELAAVLVEP
1710 1720 1730 1740 1750
VQSRRPDLQP ESFLKELRAI TQQSGTALIM DEIITGFRIG LGGAQEWFDI
1760 1770 1780 1790 1800
QADLVTYGKI IGGGQPLGIV AGKAEFMNTI DGGTWQYGDD SYPTDEAKRT
1810 1820 1830 1840 1850
FVAGTFNTHP LTMRMSLAVL RYLQAEGETL YERLNQKTTY LVDQLNSYFE
1860 1870 1880 1890 1900
QSQVPIRMVQ FGSLFRFVSS VDNDLFFYHL NYKGVYVWEG RNCFLSTAHT
1910 1920 1930 1940 1950
SDDIAYIIQA VQETVKDLRR GGFIPEGPDS PNDGGHKEPE TYELSPEQKQ
1960 1970 1980 1990 2000
LAVVSQYGND ASAALNQSIM LKVKGAVQHT LLKQAVRNIV KRHDALRTVI
2010 2020 2030 2040 2050
HVDDEVQQVQ ARINVEIPII DFTGYPNEQR ESEVQKWLTE DAKRPFHFHE
2060 2070 2080 2090 2100
QKPLFRVHVL TSKQDEHLIV LTFHHIIADG WSIAVFVQEL ESTYAAIVQG
2110 2120 2130 2140 2150
SPLPSHEVVS FRQYLDWQQA QIENGHYEEG IRYWRQYLSE PIPQAILTSM
2160 2170 2180 2190 2200
SSSRYPHGYE GDRYTVTLDR PLSKAIKSLS IRMKNSVFAT ILGAFHLFLQ
2210 2220 2230 2240 2250
QLTKQAGLVI GIPTAGQLHM KQPMLVGNCV NMVPVKNTAS SESTLADYLG
2260 2270 2280 2290 2300
HMKENMDQVM RHQDVPMTLV ASQLPHDQMP DMRIIFNLDR PFRKLHFGQM
2310 2320 2330 2340 2350
EAELIAYPIK CISYDLFLNV TEFDQEYVLD FDFNTSVISS EIMNKWGTGF
2360 2370 2380 2390 2400
VNLLKKMVEG DSASLDSLKM FSKEDQHDLL ELYADHQLRI SSTLDHKGVR
2410 2420 2430 2440 2450
AVYEEPENET ELQIAQIWAE LLGLEKVGRS DHFLSLGGNS LKATLMLSKI
2460 2470 2480 2490 2500
QQTFNQKVSI GQFFSHQTVK ELANFIRGEK NVKYPPMKPV EQKAFYRTSP
2510 2520 2530 2540 2550
AQQRVYFLHQ MEPNQVSQNM FGQISIIGKY DEKALIASLQ QVMQRHEAFR
2560 2570 2580 2590 2600
TSFHIIDGEI VQQIAGELDF NVRVHSMDRE EFEAYADGYV KPFRLEQAPL
2610 2620 2630 2640 2650
VRAELIKVDN EQAELLIDMH HIISDGYSMS ILTNELFALY HGNPLPEIPF
2660 2670 2680 2690 2700
EYKDFAEWQN QLLIGEVMEQ QEEYWLEQFK QEVPILQLPA DGSRAMEWSS
2710 2720 2730 2740 2750
EGQRVTCSLQ SSLIRSLQEM AQQKGTTLYM VLLAAYNVLL HKYTGQEDIV
2760 2770 2780 2790 2800
VGTPVSGRNQ PNIESMIGIF IQTMGIRTKP QANKRFTDYL DEVKRQTLDA
2810 2820 2830 2840 2850
FENQDYPFDW LVEKVNVQRE TTGKSLFNTM FVYQNIEFQE IHQDGCTFRV
2860 2870 2880 2890 2900
KERNPGVSLY DLMLTIEDAE KQLDIHFDFN PNQFEQETIE QIIRHYTSLL
2910 2920 2930 2940 2950
DSLVKEPEKS LSSVPMLSDI ERHQLLMGCN DTETPFPHND TVCQWFETQA
2960 2970 2980 2990 3000
EQRPDDEAVI FGNERCTYGQ LNERVNQLAR TLRTKGVQAD QFVAIICPHR
3010 3020 3030 3040 3050
IELIVGILAV LKAGGAYVPI DPEYPEDRIQ YMLKDSEAKI VLAQLDLHKH
3060 3070 3080 3090 3100
LTFDADVVLL DEESSYHEDR SNLEPTCGAN DLAYMIYTSG STGNPKGVLI
3110 3120 3130 3140 3150
EHRGLANYIE WAKEVYVNDE KTNFPLYSSI SFDLTVTSIF TPLVTGNTII
3160 3170 3180 3190 3200
VFDGEDKSAV LSTIMQDPRI DIIKLTPAHL HVLKEMKIAD GTTIRKMIVG
3210 3220 3230 3240 3250
GENLSTRLAQ SVSEQFKGQL DIFNEYGPTE AVVGCMIYRY DTKRDRREFV
3260 3270 3280 3290 3300
PIGSPAANTS IYVLDASMNL VPVGVPGEMY IGGAGVARGY WNRPDLTAEK
3310 3320 3330 3340 3350
FVHNPFAPGT IMYKTGDLAK RLRDGNLIYL GRIDEQVKIR GHRIELGEVE
3360 3370 3380 3390 3400
AAMHKVEAVQ KAVVLAREEE DGLQQLCAYY VSNKPITIAE IREQLSLELP
3410 3420 3430 3440 3450
DYMVPSHYIQ LEQLPLTSNG KINRKALPAP EVSLEQIAEY VPPGNEVESK
3460 3470 3480 3490 3500
LAVLWQEMLG IHRVGIKHNF FDLGGNSIRA TALAARIHKE LDVNLSVKDI
3510 3520 3530 3540 3550
FKFPTIEQLA NMALRMEKIR YVSIPSAQKI SYYPVSSAQK RMYLLSHTEG
3560 3570 3580 3590 3600
GELTYNMTGA MSVEGAIDLE RLTAAFQKLI ERHEVLRTSF ELYEGEPAQR
3610 3620 3630 3640 3650
IHPSIEFTIE QIQAREEEVE DHVLDFIKSF DLAKPPLMRV GLIELTPEKH
3660 3670 3680 3690 3700
VLLVDMHHII SDGVSMNILM KDLNQFYKGI EPDPLPIQYK DYAVWQQTEA
3710 3720 3730 3740 3750
QRQNIKKQEA YWLNRFHDEI PVLDMPTDYE RPAIRDYEGE SFEFLIPIEL
3760 3770 3780 3790 3800
KQRLSQMEEA TGTTLYMILM AAYTILLSKY SGQEDIVVGT PVSGRSHMDV
3810 3820 3830 3840 3850
ESVVGMFVNT LVIRNHPAGR KIFEDYLNEV KENMLNAYQN QDYPLEELIQ
3860 3870 3880 3890 3900
HVHLLKDSSR NPLFDTMFVL QNLDQVELNL DSLRFTPYKL HHTVAKFDLT
3910 3920 3930 3940 3950
LSIQTDQDKH HGLFEYSKKL FKKSRIEALS KDYLHILSVI SQQPSIQIEH
3960 3970
IELSGSTAED DNLIHSIELN F
Length:3,971
Mass (Da):449,268
Last modified:May 1, 2000 - v1
Checksum:iC8BF27D87F10065B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF184956 Genomic DNA. Translation: AAF08795.1.
PIRiT44806.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF184956 Genomic DNA. Translation: AAF08795.1 .
PIRi T44806.

3D structure databases

ProteinModelPortali Q9R9J1.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.1200.10. 4 hits.
3.40.47.10. 2 hits.
3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProi IPR010071. AA_adenyl_domain.
IPR009081. Acyl_carrier_prot-like.
IPR005814. Aminotrans_3.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR001242. Condensatn.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR020806. PKS_PP-bd.
IPR006162. PPantetheine_attach_site.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF00202. Aminotran_3. 1 hit.
PF00501. AMP-binding. 2 hits.
PF13193. AMP-binding_C. 1 hit.
PF00668. Condensation. 3 hits.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF00550. PP-binding. 4 hits.
[Graphical view ]
SMARTi SM00823. PKS_PP. 4 hits.
[Graphical view ]
SUPFAMi SSF47336. SSF47336. 4 hits.
SSF53383. SSF53383. 1 hit.
SSF53901. SSF53901. 2 hits.
TIGRFAMsi TIGR01733. AA-adenyl-dom. 1 hit.
PROSITEi PS00600. AA_TRANSFER_CLASS_3. 1 hit.
PS50075. ACP_DOMAIN. 4 hits.
PS00455. AMP_BINDING. 2 hits.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The mycosubtilin synthetase of Bacillus subtilis ATCC6633: a multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase."
    Duitman E.H., Hamoen L.W., Rembold M., Venema G., Seitz H., Saenger W., Bernhard F., Reinhardt R., Schmidt M., Ullrich C., Stein T., Leenders F., Vater J.
    Proc. Natl. Acad. Sci. U.S.A. 96:13294-13299(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 6633 / PCI 219 / NRS 231.
  2. "Characterization of a new tailoring domain in polyketide biogenesis: the amine transferase domain of MycA in the mycosubtilin gene cluster."
    Aron Z.D., Dorrestein P.C., Blackhall J.R., Kelleher N.L., Walsh C.T.
    J. Am. Chem. Soc. 127:14986-14987(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, COFACTOR OF AMINOTRANSFERASE DOMAIN.

Entry informationi

Entry nameiMYCA_BACIU
AccessioniPrimary (citable) accession number: Q9R9J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: May 1, 2000
Last modified: November 26, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3