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Q9R9J1 (MYCA_BACIU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mycosubtilin synthase subunit A

EC=2.3.1.-

Including the following 2 domains:

  1. Glutamate-1-semialdehyde aminotransferase
    Short name=GSA-AT
  2. ATP-dependent asparagine adenylase 1
    Short name=AsnA 1
    Alternative name(s):
    Asparagine activase 1
Gene names
Name:mycA
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length3971 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This protein is a multifunctional enzyme, able to activate a long chain fatty acid and link it with the amino acid Asn as part of the synthesis of mycosubtilin. The activation sites consist of individual domains. Ref.2

Cofactor

Pyridoxal phosphate. Ref.2

Binds 2 phosphopantetheines covalently Potential. Ref.2

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Contains 4 acyl carrier domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 39713971Mycosubtilin synthase subunit A
PRO_0000360848

Regions

Domain583 – 65068Acyl carrier 1
Domain1292 – 136170Acyl carrier 2
Domain2410 – 247768Acyl carrier 3
Domain3447 – 351468Acyl carrier 4
Region160 – 479320Acyl-CoA ligase
Region672 – 1095424Beta-ketoacyl synthase
Region1529 – 1856328GSA-AT
Region1938 – 2240303Condensation 1
Region2492 – 2781290Condensation 2
Region2937 – 3823887Domain 1 (asparagine-activating)
Region2967 – 3364398Adenylation 1
Region3529 – 3818290Condensation 3

Sites

Active site8431For beta-ketoacyl synthase activity By similarity

Amino acid modifications

Modified residue6131O-(pantetheine 4'-phosphoryl)serine By similarity
Modified residue13241O-(pantetheine 4'-phosphoryl)serine By similarity
Modified residue17591N6-(pyridoxal phosphate)lysine By similarity
Modified residue24401O-(pantetheine 4'-phosphoryl)serine By similarity
Modified residue34771O-(pantetheine 4'-phosphoryl)serine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9R9J1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: C8BF27D87F10065B

FASTA3,971449,268
        10         20         30         40         50         60 
MYTSQFQTLV DVIRNRSNIS DRGIRFIESD KIETFVSYRQ LFDEAQGFLG YLQHIGIQPK 

        70         80         90        100        110        120 
QEIVFQIQEN KSFVVAFWAC LLGGMIPVPV SIGEDNDHKL KVWRIWNILN NPFLLASETV 

       130        140        150        160        170        180 
LDKMKKFAAD HDLQDFHHQL IEKSDIIQDR IYDHPASQYE PEADELAFIQ FSSGSTGDPK 

       190        200        210        220        230        240 
GVMLTHHNLI HNTCAIRNAL AIDLKDTLLS WMPLTHDMGL IACHLVPALA GINQNLMPTE 

       250        260        270        280        290        300 
LFIRRPILWM KKAHEHKASI LSSPNFGYNY FLKFLKDNKS YDWDLSHIRV IANGAEPILP 

       310        320        330        340        350        360 
ELCDEFLTRC AAFNMKRSAI LNVYGLAEAS VGATFSNIGE RFVPVYLHRD HLNLGERAVE 

       370        380        390        400        410        420 
VSKEDQNCAS FVEVGKPIDY CQIRICNEAN EGLEDGFIGH IQIKGENVTQ GYYNNPESTN 

       430        440        450        460        470        480 
RALTPDGWVK TGDLGFIRKG NLVVTGREKD IIFVNGKNVY PHDIERVAIE LEDIDLGRVA 

       490        500        510        520        530        540 
ACGVYDQETR SREIVLFAVY KKSADRFAPL VKDIKKHLYQ RGGWSIKEIL PIRKLPKTTS 

       550        560        570        580        590        600 
GKVKRYELAE QYESGKFALE STKIKEFLEG HSTEPVQTPI HEIETALLSI FSEVMDGKKI 

       610        620        630        640        650        660 
HLNDHYFDMG ATSLQLSQIA ERIEQKFGCE LTVADLFTYP SIADLAAFLV ENHSEIKQTD 

       670        680        690        700        710        720 
TAKPSRSSSK DIAIIGMSLN VPGASNKSDF WHLLENGEHG IREYPAPRVK DAIDYLRSIK 

       730        740        750        760        770        780 
SERNEKQFVR GGYLDEIDRF DYSFFGLAPK TAKFMDPNQR LFLQSAWHAI EDAGYAGDTI 

       790        800        810        820        830        840 
SGSQLGVYVG YSKVGYDYER LLSANYPEEL HHYIVGNLPS VLASRIAYFL NLKGPAVTVD 

       850        860        870        880        890        900 
TACSSSLVAV HMACKALLTG DCEMALAGGI RTSLLPMRIG LDMESSDGLT KTFSKDSDGT 

       910        920        930        940        950        960 
GSGEGVAAVL LKPLQAAIRD GDHIYGVIKG SAINQDGTTV GITAPSPAAQ TEVIEMAWKD 

       970        980        990       1000       1010       1020 
AGIAPETLSF IEAHGTGTKL GDPVEFNGLC KAFEKVTEKK QFCAIGSVKA NIGHLFEAAG 

      1030       1040       1050       1060       1070       1080 
IVGLIKSALM LNHKKIPPLA HFNKPNPLIP FHSSPFYVNQ EVMDFTPEDR PLRGGISSFG 

      1090       1100       1110       1120       1130       1140 
FSGTNAHVVL EEYTPESEYA PEDGNDPHLF VLSAHTEASL YELTHQYRQY ISDDSQSSLR 

      1150       1160       1170       1180       1190       1200 
SICYTASTGR AHLDYCLAMI VSSNQELIDK LTSLIQGERN LPQVHFGYKN IKEMQPAEKD 

      1210       1220       1230       1240       1250       1260 
NLSKQISDLM QHRPCTKDER ITWLNRIAEL YVQRAVIDWR AVYSNEVVQK TPLPLYPFER 

      1270       1280       1290       1300       1310       1320 
NRCWVEAVYE SAKERKEKGE VALDINHTKT HIESFLKTVI SNASGIRADE IDSNAHFIGF 

      1330       1340       1350       1360       1370       1380 
GLDSIMLTQV KKAIADEFNV DIPMERFFDT MNNIESVVDY LAENVPSAAS TPPQESVTAQ 

      1390       1400       1410       1420       1430       1440 
EELVISGAQP ELEHQEHMLD KIIASQNQLI QQTLQAQLDS FNLLRNNSHF VSKESEISQD 

      1450       1460       1470       1480       1490       1500 
KTSLSPKSVT AKKNSAQEAK PYIPFQRQTL NEQVNYTPQQ RQYLESFIEK YVDKTKGSKQ 

      1510       1520       1530       1540       1550       1560 
YTDETRFAHA NNRNLSSFRS YWKEMVYPII AERSDGSRMW DIDGNEYIDI TMGFGVNLFG 

      1570       1580       1590       1600       1610       1620 
HHPSFITQTV VDSTHSALPP LGPMSNVAGE VADRIRACTG VERVAFYNSG TEAVMVALRL 

      1630       1640       1650       1660       1670       1680 
ARAATGRTKV VVFAGSYHGT FDGVLGVANT KGGAEPANPL APGIPQSFMN DLIILHYNHP 

      1690       1700       1710       1720       1730       1740 
DSLDVIRNLG NELAAVLVEP VQSRRPDLQP ESFLKELRAI TQQSGTALIM DEIITGFRIG 

      1750       1760       1770       1780       1790       1800 
LGGAQEWFDI QADLVTYGKI IGGGQPLGIV AGKAEFMNTI DGGTWQYGDD SYPTDEAKRT 

      1810       1820       1830       1840       1850       1860 
FVAGTFNTHP LTMRMSLAVL RYLQAEGETL YERLNQKTTY LVDQLNSYFE QSQVPIRMVQ 

      1870       1880       1890       1900       1910       1920 
FGSLFRFVSS VDNDLFFYHL NYKGVYVWEG RNCFLSTAHT SDDIAYIIQA VQETVKDLRR 

      1930       1940       1950       1960       1970       1980 
GGFIPEGPDS PNDGGHKEPE TYELSPEQKQ LAVVSQYGND ASAALNQSIM LKVKGAVQHT 

      1990       2000       2010       2020       2030       2040 
LLKQAVRNIV KRHDALRTVI HVDDEVQQVQ ARINVEIPII DFTGYPNEQR ESEVQKWLTE 

      2050       2060       2070       2080       2090       2100 
DAKRPFHFHE QKPLFRVHVL TSKQDEHLIV LTFHHIIADG WSIAVFVQEL ESTYAAIVQG 

      2110       2120       2130       2140       2150       2160 
SPLPSHEVVS FRQYLDWQQA QIENGHYEEG IRYWRQYLSE PIPQAILTSM SSSRYPHGYE 

      2170       2180       2190       2200       2210       2220 
GDRYTVTLDR PLSKAIKSLS IRMKNSVFAT ILGAFHLFLQ QLTKQAGLVI GIPTAGQLHM 

      2230       2240       2250       2260       2270       2280 
KQPMLVGNCV NMVPVKNTAS SESTLADYLG HMKENMDQVM RHQDVPMTLV ASQLPHDQMP 

      2290       2300       2310       2320       2330       2340 
DMRIIFNLDR PFRKLHFGQM EAELIAYPIK CISYDLFLNV TEFDQEYVLD FDFNTSVISS 

      2350       2360       2370       2380       2390       2400 
EIMNKWGTGF VNLLKKMVEG DSASLDSLKM FSKEDQHDLL ELYADHQLRI SSTLDHKGVR 

      2410       2420       2430       2440       2450       2460 
AVYEEPENET ELQIAQIWAE LLGLEKVGRS DHFLSLGGNS LKATLMLSKI QQTFNQKVSI 

      2470       2480       2490       2500       2510       2520 
GQFFSHQTVK ELANFIRGEK NVKYPPMKPV EQKAFYRTSP AQQRVYFLHQ MEPNQVSQNM 

      2530       2540       2550       2560       2570       2580 
FGQISIIGKY DEKALIASLQ QVMQRHEAFR TSFHIIDGEI VQQIAGELDF NVRVHSMDRE 

      2590       2600       2610       2620       2630       2640 
EFEAYADGYV KPFRLEQAPL VRAELIKVDN EQAELLIDMH HIISDGYSMS ILTNELFALY 

      2650       2660       2670       2680       2690       2700 
HGNPLPEIPF EYKDFAEWQN QLLIGEVMEQ QEEYWLEQFK QEVPILQLPA DGSRAMEWSS 

      2710       2720       2730       2740       2750       2760 
EGQRVTCSLQ SSLIRSLQEM AQQKGTTLYM VLLAAYNVLL HKYTGQEDIV VGTPVSGRNQ 

      2770       2780       2790       2800       2810       2820 
PNIESMIGIF IQTMGIRTKP QANKRFTDYL DEVKRQTLDA FENQDYPFDW LVEKVNVQRE 

      2830       2840       2850       2860       2870       2880 
TTGKSLFNTM FVYQNIEFQE IHQDGCTFRV KERNPGVSLY DLMLTIEDAE KQLDIHFDFN 

      2890       2900       2910       2920       2930       2940 
PNQFEQETIE QIIRHYTSLL DSLVKEPEKS LSSVPMLSDI ERHQLLMGCN DTETPFPHND 

      2950       2960       2970       2980       2990       3000 
TVCQWFETQA EQRPDDEAVI FGNERCTYGQ LNERVNQLAR TLRTKGVQAD QFVAIICPHR 

      3010       3020       3030       3040       3050       3060 
IELIVGILAV LKAGGAYVPI DPEYPEDRIQ YMLKDSEAKI VLAQLDLHKH LTFDADVVLL 

      3070       3080       3090       3100       3110       3120 
DEESSYHEDR SNLEPTCGAN DLAYMIYTSG STGNPKGVLI EHRGLANYIE WAKEVYVNDE 

      3130       3140       3150       3160       3170       3180 
KTNFPLYSSI SFDLTVTSIF TPLVTGNTII VFDGEDKSAV LSTIMQDPRI DIIKLTPAHL 

      3190       3200       3210       3220       3230       3240 
HVLKEMKIAD GTTIRKMIVG GENLSTRLAQ SVSEQFKGQL DIFNEYGPTE AVVGCMIYRY 

      3250       3260       3270       3280       3290       3300 
DTKRDRREFV PIGSPAANTS IYVLDASMNL VPVGVPGEMY IGGAGVARGY WNRPDLTAEK 

      3310       3320       3330       3340       3350       3360 
FVHNPFAPGT IMYKTGDLAK RLRDGNLIYL GRIDEQVKIR GHRIELGEVE AAMHKVEAVQ 

      3370       3380       3390       3400       3410       3420 
KAVVLAREEE DGLQQLCAYY VSNKPITIAE IREQLSLELP DYMVPSHYIQ LEQLPLTSNG 

      3430       3440       3450       3460       3470       3480 
KINRKALPAP EVSLEQIAEY VPPGNEVESK LAVLWQEMLG IHRVGIKHNF FDLGGNSIRA 

      3490       3500       3510       3520       3530       3540 
TALAARIHKE LDVNLSVKDI FKFPTIEQLA NMALRMEKIR YVSIPSAQKI SYYPVSSAQK 

      3550       3560       3570       3580       3590       3600 
RMYLLSHTEG GELTYNMTGA MSVEGAIDLE RLTAAFQKLI ERHEVLRTSF ELYEGEPAQR 

      3610       3620       3630       3640       3650       3660 
IHPSIEFTIE QIQAREEEVE DHVLDFIKSF DLAKPPLMRV GLIELTPEKH VLLVDMHHII 

      3670       3680       3690       3700       3710       3720 
SDGVSMNILM KDLNQFYKGI EPDPLPIQYK DYAVWQQTEA QRQNIKKQEA YWLNRFHDEI 

      3730       3740       3750       3760       3770       3780 
PVLDMPTDYE RPAIRDYEGE SFEFLIPIEL KQRLSQMEEA TGTTLYMILM AAYTILLSKY 

      3790       3800       3810       3820       3830       3840 
SGQEDIVVGT PVSGRSHMDV ESVVGMFVNT LVIRNHPAGR KIFEDYLNEV KENMLNAYQN 

      3850       3860       3870       3880       3890       3900 
QDYPLEELIQ HVHLLKDSSR NPLFDTMFVL QNLDQVELNL DSLRFTPYKL HHTVAKFDLT 

      3910       3920       3930       3940       3950       3960 
LSIQTDQDKH HGLFEYSKKL FKKSRIEALS KDYLHILSVI SQQPSIQIEH IELSGSTAED 

      3970 
DNLIHSIELN F 

« Hide

References

[1]"The mycosubtilin synthetase of Bacillus subtilis ATCC6633: a multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase."
Duitman E.H., Hamoen L.W., Rembold M., Venema G., Seitz H., Saenger W., Bernhard F., Reinhardt R., Schmidt M., Ullrich C., Stein T., Leenders F., Vater J.
Proc. Natl. Acad. Sci. U.S.A. 96:13294-13299(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 6633 / PCI 219 / NRS 231.
[2]"Characterization of a new tailoring domain in polyketide biogenesis: the amine transferase domain of MycA in the mycosubtilin gene cluster."
Aron Z.D., Dorrestein P.C., Blackhall J.R., Kelleher N.L., Walsh C.T.
J. Am. Chem. Soc. 127:14986-14987(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, COFACTOR OF AMINOTRANSFERASE DOMAIN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF184956 Genomic DNA. Translation: AAF08795.1.
PIRT44806.

3D structure databases

ProteinModelPortalQ9R9J1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.1200.10. 4 hits.
3.40.47.10. 2 hits.
3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProIPR010071. AA_adenyl_domain.
IPR009081. Acyl_carrier_prot-like.
IPR005814. Aminotrans_3.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR001242. Condensatn.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR020806. PKS_PP-bd.
IPR006162. PPantetheine_attach_site.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF00202. Aminotran_3. 1 hit.
PF00501. AMP-binding. 2 hits.
PF13193. AMP-binding_C. 1 hit.
PF00668. Condensation. 3 hits.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF00550. PP-binding. 4 hits.
[Graphical view]
SMARTSM00823. PKS_PP. 4 hits.
[Graphical view]
SUPFAMSSF47336. SSF47336. 4 hits.
SSF53383. SSF53383. 1 hit.
SSF53901. SSF53901. 2 hits.
TIGRFAMsTIGR01733. AA-adenyl-dom. 1 hit.
PROSITEPS00600. AA_TRANSFER_CLASS_3. 1 hit.
PS50075. ACP_DOMAIN. 4 hits.
PS00455. AMP_BINDING. 2 hits.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMYCA_BACIU
AccessionPrimary (citable) accession number: Q9R9J1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: May 1, 2000
Last modified: February 19, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families