Q9R9J0 (MYCB_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mycosubtilin synthase subunit B
EC=2.3.1.-

Including the following 4 domains:

1. ATP-dependent tyrosine adenylase
   Short name=TyrA
   Alternative name(s):
   Tyrosine activase
2. ATP-dependent asparagine adenylase 2
   Short name=AsnA 2
   Alternative name(s):
   Asparagine activase 2
3. ATP-dependent glutamine adenylase
   Short name=GlnA
   Alternative name(s):
   Glutamine activase
4. ATP-dependent proline adenylase
   Short name=ProA
   Alternative name(s):
   Proline activase

Gene names

Name:

mycB

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	5369 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein is a multifunctional enzyme, able to activate and polymerize the amino acids Tyr, Asn, Gln and Pro as part of the synthesis of mycosubtilin. The Asn and Gln residues are further epimerized to the D-isomer form. The activation sites for these amino acids consist of individual domains.
Cofactor	Binds 4 phosphopantetheines covalently Potential.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family. Contains 4 acyl carrier domains.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Domain	Repeat
Ligand	Phosphopantetheine
Molecular function	Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acyl carrier activity Inferred from electronic annotation. Source: InterPro cofactor binding Inferred from electronic annotation. Source: InterPro ligase activity Inferred from electronic annotation. Source: InterPro phosphopantetheine binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 5369	5369	Mycosubtilin synthase subunit B		PRO_0000360849
Regions
	Domain	769 – 835	67	Acyl carrier 1
	Domain	2280 – 2346	67	Acyl carrier 2
	Domain	3806 – 3873	68	Acyl carrier 3
	Domain	4845 – 4912	68	Acyl carrier 4
	Region	260 – 1620	1361	Domain 1 (D-tyrosine-activating)
	Region	290 – 686	397	Adenylation 1
	Region	846 – 1305	460	Epimerization 1
	Region	1315 – 1615	301	Condensation 1
	Region	1770 – 3132	1363	Domain 2 (D-asparagine-activating)
	Region	1800 – 2197	398	Adenylation 2
	Region	2357 – 2816	460	Epimerization 2
	Region	2826 – 3127	302	Condensation 2
	Region	3281 – 4182	902	Domain 3 (glutamine-activating)
	Region	3311 – 3723	413	Adenylation 3
	Region	3888 – 4177	290	Condensation 3
	Region	4334 – 5221	888	Domain 4 (proline-activating)
	Region	4364 – 4762	399	Adenylation 4
	Region	4927 – 5216	290	Condensation 4
	Compositional bias	101 – 104	4	Poly-Ser
Amino acid modifications
	Modified residue	799	1	O-(pantetheine 4'-phosphoryl)serine By similarity
	Modified residue	2310	1	O-(pantetheine 4'-phosphoryl)serine By similarity
	Modified residue	3836	1	O-(pantetheine 4'-phosphoryl)serine By similarity
	Modified residue	4875	1	O-(pantetheine 4'-phosphoryl)serine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9R9J0 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: E99AD8C33B45EBE2	FASTA	5,369	612,337
	10 20 30 40 50 60 MSVFKNQVTY WANLFDEEDS LSVIPYFKTA ENASLTRVGY QEKSIYRSLS PEVSQRILTM 70 80 90 100 110 120 ANHSEMATYL ILLAGIECLL YKYTDRASTI LGIPTVSKQK SSSSTVNTIV LLKNTLSCQS 130 140 150 160 170 180 TFKTVFEQLK KAVNDSLKNQ NLPFRKIVQH VNVQYDNENI PLIHTVVSLN EIHSLQFKED 190 200 210 220 230 240 IATDTLFHFD LENSEIHLKL IYNGNLYDED YMDQMVSHLN QLLSVILFQP QAAIHTAEGI 250 260 270 280 290 300 PEAVKQQILF DFNDTAADYS GNKTVSQLFE EQAERTPDHV AVKFVNNHMT YRELNEKSNR 310 320 330 340 350 360 LARTLRNYGV QADTLVAIMA ERSLEMIVSI MAIWKAGGAY VPLDPEYPEE RLQYVLNDAN 370 380 390 400 410 420 ADVLVVQRHF KNSLVFDGPM IDLNDETSYH ADCSLLSPIA EHSHLAYVIY TSGTTGKPKG 430 440 450 460 470 480 VMVEHGGIVN SLQWKKAFFK HSAEDRVLVL YPYVFDAFIL NFFGPLISGA ALYLLPNEDN 490 500 510 520 530 540 KDLFAIQNVL KLERITHFST SPRLLQAMTE QMNAEDFYHV QHVVVGGEKL EPDTVERLFS 550 560 570 580 590 600 LQPQIRINNE YGPTENSVVS TFQPVYSADE QITIGKPVAN HQAYILGAHR QIQPIGVPGE 610 620 630 640 650 660 LYVGGSGVAR GYLNQPDLTE EKFVDHLLIP RRKMYKTGDL ARWLPDGRIE YLGRIDHQVK 670 680 690 700 710 720 IRGYRIELGE VEAALSNLEE VRETTVESRE GIDGTKQLYA YYVGEPSLSA GQFREILSRE 730 740 750 760 770 780 LPDYMIPSYF IHLERIPLTS NGKIDLKALP VADEKTRMEN EYIAPQNSIE ELLASIWQEV 790 800 810 820 830 840 LGTERIGILD NFFDFGGDSI KSIQVSSRLY QSGYKIDMKH LFTYPTIAEL SPFVEPVGRM 850 860 870 880 890 900 ADQGEVKGRT SLTPIQHWFF EQNMPHANHY NQAVMLYSAQ GFKETPLRRA IESIAVHHDA 910 920 930 940 950 960 LRMVFEETPN GYTARIAGTE ESKLYQLEVM NCKEDADPAH AVANKANQIQ SSMELSQGPL 970 980 990 1000 1010 1020 MKLGLFKCAD GDHLLIAIHH LVIDGVSWRI LLEDFASSYE QAERGQMVRL PQKTDSFPFW 1030 1040 1050 1060 1070 1080 AEQLSKYAQE TDREQELAYW NELARLELEP LPKDNAVEDS LLKDSGDVTI QWTREETQQL 1090 1100 1110 1120 1130 1140 LKQANRAYNT EINDLLLTSL GLAVHRWTGM EEVVVNLEGH GREPVIPDVD ITRTVGWFTS 1150 1160 1170 1180 1190 1200 QYPVVLKMEA GKELSQRIKT VKEGLRRVPN KGMNYSVIQY LSGRAEADSL QLHPEIRFNY 1210 1220 1230 1240 1250 1260 LGQFDQDLQQ HALQISPYST GVSLNENQPR TAVLDLNGMV AEGKLSLSLS YSHKQYERST 1270 1280 1290 1300 1310 1320 MEQFARSLKE SLQEIIVHCV NQQQTSLTPS DVLLKDIKID ELEELLDQTR ELGEVENIYP 1330 1340 1350 1360 1370 1380 LTPMQKGMLF HSLFDSHSGA YFQQTMFELH GDLDIDSFSK SLDDLSKRYE IFRTNFLKGK 1390 1400 1410 1420 1430 1440 DQPLQIIFKT KKIGFQFIDL REMDQARQDD RICAYAKEDK LRGFDLAKDV LMRVIVLRTD 1450 1460 1470 1480 1490 1500 DTTYRFIWSF HHILMDGWCL PLITKEIFDH YFALLQQKQP EQAAITPYSQ YIEWLDRQDA 1510 1520 1530 1540 1550 1560 EEAKRYWDQY LEGYEEQTVL LKDSHQAEDE HYFPEKVSCA IDTDLTLKMK QTASKHHVTL 1570 1580 1590 1600 1610 1620 NTLLQTAWGI LLQKYNRSRD VVFGSVVSGR PSAIPNVETM IGLFINTIPV RIQCEAGTTF 1630 1640 1650 1660 1670 1680 AELMKRTQER AVASQTYETY PLYDIQALTT QKQNLITHLM IFENYPVDQY MESIGQHNES 1690 1700 1710 1720 1730 1740 PIDISNVKME EQTNYHLNVT VIPGDVININ FEYNAKVYDR ESMERVRGHL LQILHQVVAD 1750 1760 1770 1780 1790 1800 ADIRVEQLEM LTEGEKRQLL QNLNDTAAPY PQSTVGQWFE AQSQQIPDQA AVIDGDKQIT 1810 1820 1830 1840 1850 1860 YRQLNERANR LARTLRARGV QADQPVATIS RNSIELVTGI LAILKAGGAY VPIDPEYPQD 1870 1880 1890 1900 1910 1920 RIQYILEDSK AGIILMPRDV RQQITYEGVV ILLDEESSYH EEAFNLEPLS NANHLAYVIY 1930 1940 1950 1960 1970 1980 TSGSTGKPKG VLIEHRGLSN YIWWAKEVYV KNEKTNFPLY SSISFDLTVT SIFTPLVTGN 1990 2000 2010 2020 2030 2040 TIIVYDGEDK TALLSSIVQD QRVDIIKLTP AHLHVLKAMN IANKIAIRKM IVGGENLSTQ 2050 2060 2070 2080 2090 2100 LAQSIHEQFD GQIEICNEYG PTETVVGCML YRYDAVKDRR ESVPIGTAAA NTSIYVLDED 2110 2120 2130 2140 2150 2160 MKPVPIGVPG EMYISGAGVA RGYLNRPELT AEKFVENPFV TGERMYKTGD LAKWLPDGNI 2170 2180 2190 2200 2210 2220 EYLGRMDEQV KIRGFRIELG EIETAMLQAE EIKEAVVTAR EDVHGLKQLC GYYVSSQPIT 2230 2240 2250 2260 2270 2280 VSQIREQLSQ SLPGYMIPAY FIKLEKMPLT PNGKINQKEL PTPDLQLQDR VAYKPPRTQV 2290 2300 2310 2320 2330 2340 EQLLVSTWES VLGAEKIGIL DNFFDLGGDS IKSIQVSSRL NQLGYKMEIK HLFQYATIAE 2350 2360 2370 2380 2390 2400 LSPHIEQNVR IPDQDEVKGK VSLTPIQHWF FEQTTTDPHY YNQAVMLYAP QGFSGIAASP 2410 2420 2430 2440 2450 2460 NATKAWRAPR CVRMTFQATE HGYEAWNAEI TQSELYHLDV INLKTEADPG PAIEAKANQI 2470 2480 2490 2500 2510 2520 QSSMQLSNGP LMKAGLFQCA DGDHLLIAIH HLIVDGISWR ILMEDIVSGY KQAENGQDIQ 2530 2540 2550 2560 2570 2580 LPYKTDSFRL WAEKLSAYAQ SDAIKQEQEY WARIEQTDVK PLPKDFQESH AFSIDSETVT 2590 2600 2610 2620 2630 2640 VEWTKEETEQ LLKQANRAYN TEINDLLLSS LGLSISHWSG LEQIALHLEG HGREQVIPNM 2650 2660 2670 2680 2690 2700 DISRTVGWFT SLFPVVLHIE PGKEISHYIK TAKEELRQIP HKGIGYGVLR YLSGSTTPLP 2710 2720 2730 2740 2750 2760 AKMTPEISFN YLGQFDQDIQ NQAVQLSSYS CGSDSSGNQI RPYVLNINGM IVNDRLMVTI 2770 2780 2790 2800 2810 2820 SYSTKQYAKE TIDQLSAIIQ NNLRTVIEHC VHKEQTELTP SDILLKGMAI DELDQLLIQL 2830 2840 2850 2860 2870 2880 PDAGEIENVY PLTPMQKGML FHSLLDEDSN SYFEQASFDL QGELKIDRFE ASLDHLFAKY 2890 2900 2910 2920 2930 2940 AVLRTRFYSG WNDIPLQIVY KTQRMKVHFT DLRDIDENQR KDEIASYQSE DKAKGFDLAR 2950 2960 2970 2980 2990 3000 DPLMRIAIFR IEERKYHLIW SFHHIVMDGW CLPLITKEAF EHYIGLQEGR ETDLAYTDPY 3010 3020 3030 3040 3050 3060 SKYIEWLDQQ DQNAAKRYWR EYLEGYKGET RILHKRPQHE RKAYAYANEI CRFNQKQTRQ 3070 3080 3090 3100 3110 3120 LQRIANQHHV TLNTLIQTLW GILLQKYSGT GDVVFGSVVS GRPAEISGVE QMIGLFINTI 3130 3140 3150 3160 3170 3180 PVRICCDEGS SFVETMKMVQ DNALASQSYD TYPLYEIQAQ TEQKQNLIDH ILIFENYPIG 3190 3200 3210 3220 3230 3240 QQVEEGHNAA ELNIMNFHME EHSHYDFNMV VIPGEQLNVH FGYNQNVYEQ SEVERISGHF 3250 3260 3270 3280 3290 3300 EQLMHQVLEH PNIKVEELEL LTQQEKEQLL SRFQAREMQY SREQTIHERF SKQAFRTPDR 3310 3320 3330 3340 3350 3360 TAVVFEGESL TYGELNKRAN QLAQALRVEG VQAGQLVGIM AERSLEMIIG IFGILKAGGA 3370 3380 3390 3400 3410 3420 YLPIDPDSPV ERIHYIARDS GINILLTHGE LPENLNFSGT CINMKEEQAY HETDINLAVP 3430 3440 3450 3460 3470 3480 CQFDQLAYCI YTSGTTGTPK GTPKGTLIEH RQVIHLIEGL RNAVYSAYDG VLHVAMLAPY 3490 3500 3510 3520 3530 3540 YFDASVQQIY ASLLLGHTLF IVPKEAVSDG EALCQYYRQH RIDVTDGTPA HLKLLVAADD 3550 3560 3570 3580 3590 3600 GEGVPLRHLL IGGEALPKTT VTKFIHLFGA DRAAPAITNV YGPTETCVDA SLFNIEVSAD 3610 3620 3630 3640 3650 3660 AWTRSQVHIP IGKPLGNNRM YILDSQQKLQ PVGVQGELYI AGDGVGRGYL NLPELTNKKF 3670 3680 3690 3700 3710 3720 VNDPFVPSGR MYRTGDLARL LPDGNIEFIE RVDHQVKIHG FRIELGEIES IMLNIPEIQE 3730 3740 3750 3760 3770 3780 AVASVLEDAD GEHYICGYYV ANKPFPTSQL RDRLTRHLPG YMIPAYFVQM DQMPLTPNGK 3790 3800 3810 3820 3830 3840 LNRNLLPEPD GKRYGDTEYV PPRNSTEMKL TKIWQDVLGL EQVGIRDNFF DIGGHSLRAT 3850 3860 3870 3880 3890 3900 TLIAKIQKQL HVQIPLRNIF QFPTIEQLAQ AIMTMEETEY ASIPLIEKRP YYPVSSAQKR 3910 3920 3930 3940 3950 3960 LYILNHLEGG ELSYNMLGLM TVKGELDRDK LQQAFDKLIH RHESLRTGFK MVDGEPVQYV 3970 3980 3990 4000 4010 4020 LDHVEFAVES YYAKEDEIDH CISQFVRAFQ LEEPPLLRVG LIELQPNHAI LMFDMHHIIS 4030 4040 4050 4060 4070 4080 DGTSMNVLIK EFVRAYQGAE LPPLRIQYKD YSVWQTGEAR LEQIQKQEDY WLELYSGDIP 4090 4100 4110 4120 4130 4140 VLHLPTDYIR PSTRDFTGAT LHFTIDEKKS EGLKQLALKT ESTLYMVLLA SYTLLLSKYS 4150 4160 4170 4180 4190 4200 GQEDIVVGSP IAGRPHADLD SIIGMFVNTL AMRNYPAKEK TFSQYLAEVK ENALKAYEHQ 4210 4220 4230 4240 4250 4260 DYPFEALVDQ LNIARDLSRN PLFDTMFVLQ NTEQEQLGMD GLTFKPYPSK HTMAKFDLTL 4270 4280 4290 4300 4310 4320 TAVEEETHIH CTMEYLTTLF KPETVERMMR HFVQLIDAII EHPEARLASL EMMRSREKNE 4330 4340 4350 4360 4370 4380 IWNLFNDTAV IDERMPTTIH QQFEQQAEDT PDRVAILFEN QTWTYRQLNE RSNQLARVLR 4390 4400 4410 4420 4430 4440 NQGVGADRVA AILTERSANM MIGILAILKA GGAFLPIDPE LPDERRAYLM EDSGADVLVT 4450 4460 4470 4480 4490 4500 CVEHTVPPSF EGSVVLLDDP LVYQGDASNL NLSYAENHLL YVIYTSGTTG KPKGVQLEHK 4510 4520 4530 4540 4550 4560 TMLNLLAYER EYTQLRFDRV LQFAAMSFDV CYQEIFSTIL SGGTLYIIDN EAKREIRELN 4570 4580 4590 4600 4610 4620 EFVKTHRIQT AFLPTAFLKL LASEKQYFEP FAECVDHIIT AGEQLIMTNT LREMMMRHQV 4630 4640 4650 4660 4670 4680 SLHNHYGPSE THVVTMCTVD PEIHQEMPPI GKPISNTEIL ILNEAGTLQP IGIVGELCIA 4690 4700 4710 4720 4730 4740 GISLARGYHN RESLTHEKFV PHPYDANKRM YKTGDLARYL PDGNIEYAGR MDHQVKIRGY 4750 4760 4770 4780 4790 4800 RIELNEVEAA LLNIEHVQEA VVLARENTEG QSDLYAYFVA EQALPISQFK EKLAQQIPGY 4810 4820 4830 4840 4850 4860 MIPSYLMQLE QMPLTSNGKV NRSALPLPAA GMQTGIDHVA PRTRLEEQLV LIWKEVLKLE 4870 4880 4890 4900 4910 4920 QVGVKDNFFD LGGHSLRGMT LVGKIHKQFN KTISLREVFQ GPTIEEMAKV IANSETCGPD 4930 4940 4950 4960 4970 4980 YIPAVEVKDV YPVSSVQKMV YLSTQIEGGE LSYNMPGILT LEGRIDMDRL QTAFHRLIQR 4990 5000 5010 5020 5030 5040 HESLRTGFEM IRGEPMQMVK PEVEFTIERY KATAEEVEEL FRTFVRPFDL SQAPLLRVGL 5050 5060 5070 5080 5090 5100 IELEQEKHIF MFDMHHIVTD GASMNIFIEE LIQLYDGKEL APLRIQYKDF TAWQQQAEQK 5110 5120 5130 5140 5150 5160 ERIKKQEDYW LDVFHEALPS FELPKDFARP QVRSFEGKRY NFVLNESVVQ GVKQLEELTG 5170 5180 5190 5200 5210 5220 STTYMILFAA YTILLAKYSG QEDIVVGTPV AGRVHDDLQH IIGMFVNTLA IRTAPAGEKT 5230 5240 5250 5260 5270 5280 FKDYVTETKE TMLKAYENQE YPFEELVEKL GVQRDLSRNP LFDTMFVLQN TEQTDIEIDS 5290 5300 5310 5320 5330 5340 LAVRPYEETH AVAKFDLQLT FEMHQHEIQG SFDYCTKLFK KRTIATLAQD YVMILSAVIQ 5350 5360 NSSIPLKEIQ LSEKVNKKEH FASVIELDF

References

[1]

"The mycosubtilin synthetase of Bacillus subtilis ATCC6633: a multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase." Duitman E.H., Hamoen L.W., Rembold M., Venema G., Seitz H., Saenger W., Bernhard F., Reinhardt R., Schmidt M., Ullrich C., Stein T., Leenders F., Vater J. Proc. Natl. Acad. Sci. U.S.A. 96:13294-13299(1999) [PubMed: 10557314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6633 / PCI 219 / NRS 231.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF184956 Genomic DNA. Translation: AAF08796.1.
PIR	T44807.
3D structure databases
HSSP	HSSP built from PDB template 1DNY based on UniProtKB O30409.
ProteinModelPortal	Q9R9J0.
SMR	Q9R9J0. Positions 3797-4313.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR010071. AA_adenyl_domain. IPR009081. Acyl_carrier_prot-like. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. IPR001242. Condensatn. IPR010060. NRPS_synth. IPR006163. Phsphopanteth-bd. IPR006162. PPantetheine_attach_site. [Graphical view]
Gene3D	G3DSA:1.10.1200.10. ACP_like. 4 hits.
Pfam	PF00501. AMP-binding. 4 hits. PF00668. Condensation. 7 hits. PF00550. PP-binding. 4 hits. [Graphical view]
SUPFAM	SSF47336. ACP_like. 4 hits.
TIGRFAMs	TIGR01733. AA-adenyl-dom. 4 hits. TIGR01720. NRPS-para261. 2 hits.
PROSITE	PS50075. ACP_DOMAIN. 4 hits. PS00455. AMP_BINDING. 3 hits. PS00012. PHOSPHOPANTETHEINE. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MYCB_BACSU

Accession

Primary (citable) accession number: Q9R9J0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	May 1, 2000
Last modified:	September 21, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program