Q9R9H8 (BBMA2_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Intracellular maltogenic amylase
EC=3.2.1.-

Gene names

Name:

bbmA

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes beta-cyclodextrin to maltose and glucose, soluble starch to maltose and glucose, and pullulan to panose with trace amounts of maltose and glucose. It is also able to hydrolyze acarbose. Can also exhibit a transglycosylation activity transferring glucose or maltose to another moiety of sugars by forming alpha-(1,6)- and alpha-(1,3)-glycosidic linkages upon the hydrolysis of substrate at concentrations of 5% or higher. Ref.1
Subunit structure	Monomer or homodimer; in equilibrium. Ref.1
Subcellular location	Cytoplasm Ref.1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. BbmA subfamily.
Biophysicochemical properties	pH dependence: Optimum pH is 7.0. Stable at pH 6.0 and about 80% of the enzyme activity remained between pH 7.0 and pH 8.0. Ref.1 Temperature dependence: Optimum temperature is 40-45 degrees Celsius.
Sequence caution	The sequence AAF23874.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	Intracellular maltogenic amylase		PRO_0000366978
Sites
	Active site	327	1	Nucleophile By similarity
	Active site	356	1	Proton donor By similarity
	Site	423	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9R9H8 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: 00EE31A2A54BE14F	FASTA	588	68,700
	10 20 30 40 50 60 MEYAAIHHQP FSTDAYSYDG RTVHIKIRTK KGDADHIRFI WGDPYEYNDG KWSANEQPMR 70 80 90 100 110 120 KIAATEMHDY WFAEVVPPFR RLQYAFVVTD DHEDIFFGSS GVCPYNEKTL ETIHYYFKFP 130 140 150 160 170 180 FVHEADTFQA PEWVKSTVWY QIFPERFANG REDLSPKNAL PWGSKDPGVN DFFGGDLQGI 190 200 210 220 230 240 VDKLDYLEDL GVNGIYLTPI FSAPSNHKYD TLDYFSIDPH FGDPEIFRTL VSQLHQRGMR 250 260 270 280 290 300 IMLDAVFNHI GSASPQWQDV VKNGDQSRYK DWFHIHSFPV TDDNYDRFAF TADMPKLNTA 310 320 330 340 350 360 NPEVQKYLLD IALYWIREFD IDGWRLDVAN EVDHVFWKTF RQAVSTEKPD VYILGEIWHS 370 380 390 400 410 420 AEPWLRGDEF HAAMNYPFTE PMIEYFADQT ISASRMAHRV NAHLMNGMKQ ANEVMFNLLD 430 440 450 460 470 480 SHDTKRLLTR CRNDEKKARA LLAFMFAQTG SPCIYYGTEI GLDGENDPLC RKCMVWEKEK 490 500 510 520 530 540 QNQDMLQFMK RLIALRKQEN TLLTEGHLEW NLLDDKNDFI SFSRTLDEKI LIYFFNQGNV 550 560 570 580 VQHISLRELN IDRNNKICDA WTEQPLHYHD VIAVQPGEFL ILSAAAPV

References

[1]

"Molecular characterization of a dimeric intracellular maltogenic amylase of Bacillus subtilis SUH4-2." Cho H.-Y., Kim Y.-W., Kim T.-J., Lee H.-S., Kim D.-Y., Kim J.-W., Lee Y.-W., Leed S.-B., Park K.-H. Biochim. Biophys. Acta 1478:333-340(2000) [PubMed: 10825545] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, SUBUNIT. Strain: SUH4-2.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF115340 Genomic DNA. Translation: AAF23874.1. Different initiation.
3D structure databases
HSSP	HSSP built from PDB template 1J0H based on UniProtKB P38940.
ProteinModelPortal	Q9R9H8.
ModBase	Search...
Protein family/group databases
CAZy	CBM34. Carbohydrate-Binding Module Family 34. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR004185. Glyco_hydro_13_lg-like_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
PANTHER	PTHR10357. Alpha_amylase. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02903. Alpha-amylase_N. 1 hit. [Graphical view]
SMART	SM00642. Aamy. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BBMA2_BACSU

Accession

Primary (citable) accession number: Q9R9H8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	June 28, 2011
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program