Reviewed, UniProtKB/Swiss-Prot Q9R708 (KATG_RHIRD)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase
      Short name=CP
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase

Gene names

Name:	katG
Synonyms:	katA

Organism

Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter)

Taxonomic identifier

358 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. Involved in tumorigenesis.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 723	723	Catalase-peroxidase HAMAP MF_01961		PRO_0000354715
Sites
	Active site	98	1	Proton acceptor By similarity
	Metal binding	266	1	Iron (heme axial ligand) By similarity
	Site	94	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	97 ↔ 225		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-251) By similarity
	Cross-link	225 ↔ 251		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-97) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9R708-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: AA531987F535B508	FASTA	723	78,741
	10 20 30 40 50 60 MDATSKPAGK CPVMHGGNTV SGKSVTEWWP NALNLDILHQ HDTKTNPLGT SFNYREALKT 70 80 90 100 110 120 LDVEALKADL RALMTDSQEW WPADWGSYVG MMARVTWHAA GSYRVTDGRG GANTGNQRFA 130 140 150 160 170 180 PLNSWPDNVN TDKGRRLLWP IKKKYGNKIS WADLIALAGT IAYDVAGLKT FGFAFGREDI 190 200 210 220 230 240 WAPEKDTYWG DEKEWLAPSD GRYGDVSKPE TLENPLAAVQ MGLIYVNPEG VNGKSDPLAT 250 260 270 280 290 300 AAQMRETFAR MGMDDEETVA LTAGGHTIGK SHGNGSAANL SPDPEAAGPE YQGLGWINTK 310 320 330 340 350 360 GRGIGRDTVV SGIEGAWTSE PTKWDNGFFD MLFKHEWTLT HSPAGASQWA PITIAEEDKP 370 380 390 400 410 420 VDVEDASIRT IPMMTDADMA LKVDPIYREI SLKFKDDQDH FSDVFARAWF KLTHRDMGPK 430 440 450 460 470 480 SRYVGPDVPA EDLIWQDPIP AGSTSYDVAA VKAKIAASGL SVADLVSTAW DSARTFRGSD 490 500 510 520 530 540 KRGGANGARI RLAPQKDWEG NEPARLSRVL SVLEPIARET GASIADVIVL AGNYGVEQAA 550 560 570 580 590 600 KAAGFDIAVP FAAGRGDASA EQTDADSFAP LEPLADGFRN WVKKDYVVSP EELLLDRAQL 610 620 630 640 650 660 LGLTAPELTV LIGGLRVIGA NYGGAAHGVF TDKPGALTTD FFTTLTDMAY SWVPTGNNLY 670 680 690 700 710 720 EIRDRKTGAA RYSATRVDLV IGSNSILRAY AEVYAQDDNR EKFARDFIAA WTKVMNADRF DLI

References

[1]	"An Agrobacterium catalase is a virulence factor involved in tumorigenesis." Xu X.Q., Pan S.Q. Mol. Microbiol. 35:407-414(2000) [PubMed: 10652101] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: A348.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB033631 Genomic DNA. Translation: BAA89349.1.
3D structure databases
HSSP	HSSP built from PDB template 1MWV based on UniProtKB Q939D2.
ModBase	Search...
Protein family/group databases
PeroxiBase	2692. AtuCP01_C58.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_RHIRD

Accession

Primary (citable) accession number: Q9R708

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	May 1, 2000
Last modified:	September 22, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)