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Reviewed, UniProtKB/Swiss-Prot Q9R4E4 (AROA_AGRSC)

Last modified September 22, 2009. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    3-phosphoshikimate 1-carboxyvinyltransferase
    EC=2.5.1.19
Alternative name(s):
    5-enolpyruvylshikimate-3-phosphate synthase
      Short name=EPSP synthase
      Short name=EPSPS
Gene names
Name: aroA
OrganismAgrobacterium sp. (strain CP4)
Taxonomic identifier268951 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

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Protein attributes

Sequence length455 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210

Pathway

Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm Probable.

Biotechnological use

Introduced by genetic manipulation and expressed in glyphosate-tolerant soybean, canola, cotton and maize by Monsanto. Developed to provide new weed-control options for farmers. Expression of this protein in plants imparts high levels of glyphosate tolerance. HAMAP MF_00210

Sequence similarities

Belongs to the EPSP synthase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4554553-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210
PRO_0000088215

Experimental info

Sequence conflict21S → L AA sequence Ref.2

Secondary structure

1 ........................................................................... 455
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Q9R4E4-1 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: 236580D08D6EF422

FASTA45547,588
        10         20         30         40         50         60 
MSHGASSRPA TARKSSGLSG TVRIPGDKSI SHRSFMFGGL ASGETRITGL LEGEDVINTG 

        70         80         90        100        110        120 
KAMQAMGARI RKEGDTWIID GVGNGGLLAP EAPLDFGNAA TGCRLTMGLV GVYDFDSTFI 

       130        140        150        160        170        180 
GDASLTKRPM GRVLNPLREM GVQVKSEDGD RLPVTLRGPK TPTPITYRVP MASAQVKSAV 

       190        200        210        220        230        240 
LLAGLNTPGI TTVIEPIMTR DHTEKMLQGF GANLTVETDA DGVRTIRLEG RGKLTGQVID 

       250        260        270        280        290        300 
VPGDPSSTAF PLVAALLVPG SDVTILNVLM NPTRTGLILT LQEMGADIEV INPRLAGGED 

       310        320        330        340        350        360 
VADLRVRSST LKGVTVPEDR APSMIDEYPI LAVAAAFAEG ATVMNGLEEL RVKESDRLSA 

       370        380        390        400        410        420 
VANGLKLNGV DCDEGETSLV VRGRPDGKGL GNASGAAVAT HLDHRIAMSF LVMGLVSENP 

       430        440        450 
VTVDDATMIA TSFPEFMDLM AGLGAKIELS DTKAA

« Hide

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References

[1]"Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases."
Barry G.F., Kishore G.M., Padgette S.R., Stallings W.C.
Patent number US5633435, 27-MAY-1997
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-28; 47-61 AND 321-333.
[2]"The expressed protein in glyphosate-tolerant soybean, 5-enolpyruvylshikimate-3-phosphate synthase from Agrobacterium sp. strain CP4, is rapidly digested in vitro and is not toxic to acutely gavaged mice."
Harrison L.A., Bailey M.R., Naylor M.W., Ream J.E., Hammond B.G., Nida D.L., Burnette B.L., Nickson T.E., Mitsky T.A., Taylor M.L., Fuchs R.L., Padgette S.R.
J. Nutr. 126:728-740(1996) [PubMed: 8598558] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-15.
+Additional computationally mapped references.

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Cross-references

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2GG4X-ray2.10A1-455[»]
2GG6X-ray1.64A1-455[»]
2GGAX-ray1.70A1-455[»]
2GGDX-ray1.70A1-455[»]
2PQBX-ray1.80A6-450[»]
2PQCX-ray1.60A6-450[»]
2PQDX-ray1.77A6-450[»]
ModBaseSearch...

Family and domain databases

HAMAPMF_00210.
[Tree]
InterProIPR016228. EPSP_synthase.
IPR001986. EPSP_synthase_core.
IPR006264. EPSP_synthase_subgroup.
[Graphical view]
Gene3DG3DSA:3.65.10.10. EPSP_synthase. 2 hits.
PfamPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF000505. EPSPS. 1 hit.
ProDomPD001867. EPSP_synth. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR01356. aroA. 1 hit.
PROSITEPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameAROA_AGRSC
AccessionPrimary (citable) accession number: Q9R4E4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: September 22, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents