Q9R435 (Q9R435_KLEPN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Extended spectrum beta-lactamase EMBL AAB84239.1

Submitted name:
Extended-spectrum TEM-type beta-lactamase variant, TEM-52 EMBL CAA73933.1

Gene names

Name:

blaTEM-52 EMBL AAB84239.1

Encoded on

Plasmid pKK50-2 Ref.1 Ref.3 EMBL AAB84239.1

Organism

Klebsiella pneumoniae EMBL AAB84239.1

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid. SAAS SAAS012338

Ontologies

Keywords
Biological process	Antibiotic resistance SAAS SAAS012338
Molecular function	Hydrolase SAAS SAAS023650
Technical term	3D-structure PDB 1HTZ Plasmid EMBL AAB84239.1
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q9R435 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 13717ACCB58F6FB6	FASTA	286	31,514
	10 20 30 40 50 60 MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP 70 80 90 100 110 120 EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VKYSPVTEKH LTDGMTVREL 130 140 150 160 170 180 CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTT 190 200 210 220 230 240 PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGASERGS 250 260 270 280 RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW

References

[1]	"Predicting the emergence of antibiotic resistance by directed evolution and structural analysis." Orencia M.C., Yoon J.S., Ness J.E., Stemmer W.P., Stevens R.C. Nat. Struct. Biol. 8:238-242(2001) [PubMed: 11224569] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 24-286.
[2]	"A novel extended-spectrum TEM-type beta-lactamase (TEM-52) associated with decreased susceptibility to moxalactam in Klebsiella pneumoniae." Poyart C., Mugnier P., Quesne G., Berche P., Trieu-Cuot P. Antimicrob. Agents Chemother. 42:108-113(1998) [PubMed: 9449269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Molecular Evolution of blaTEM of Extended Spectrum of beta-lactamase (ESBL) in Klebsiella pneumoniae isolated in Korea." Cho D.T., Shin H.S. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: KMK107 EMBL AAB84239.1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF027199 Genomic DNA. Translation: AAB84239.1.
Y13612 Genomic DNA. Translation: CAA73933.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HTZ	X-ray	2.40	A/B/C/D/E/F	24-286	[»]

ProteinModelPortal

Q9R435.

SMR

Q9R435. Positions 24-286.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]

Gene3D

G3DSA:3.40.710.10. G3DSA:3.40.710.10. 1 hit.

Pfam

PF00144. Beta-lactamase. 1 hit.
[Graphical view]

PRINTS

PR00118. BLACTAMASEA.

SUPFAM

SSF56601. PBP_transp_fold. 1 hit.

PROSITE

PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q9R435_KLEPN

Accession

Primary (citable) accession number: Q9R435

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 2000
Last sequence update:	May 1, 2000
Last modified:	July 27, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)