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Protein

Aminoglycoside N(6')-acetyltransferase type 1

Gene
N/A
Organism
Salmonella enteritidis
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of an acetyl group from acetyl-CoA to the 6'-amino group of aminoglycoside molecules conferring resistance to antibiotics containing the purpurosamine ring including amikacin, tobramycin, dibekacin and ribostamycin. Able to acetylate eukaryotic histone proteins.2 Publications

Catalytic activityi

Acetyl-CoA + kanamycin-B = CoA + N(6')-acetylkanamycin-B.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22Substrate1 Publication1
Binding sitei25Substrate2 Publications1
Binding sitei66Substrate2 Publications1
Binding sitei79Substrate2 Publications1
Binding sitei115Substrate2 Publications1
Binding sitei120Acetyl-CoA2 Publications1
Binding sitei136Substrate2 Publications1

GO - Molecular functioni

  • aminoglycoside 6'-N-acetyltransferase activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

  • acetyl-CoA metabolic process Source: UniProtKB
  • histone acetylation Source: UniProtKB
  • response to antibiotic Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoglycoside N(6')-acetyltransferase type 1By similarity (EC:2.3.1.822 Publications)
Alternative name(s):
AAC(6')-IyBy similarity
Aminoglycoside resistance proteinBy similarity
OrganismiSalmonella enteritidis
Taxonomic identifieri149539 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella

Pathology & Biotechi

Chemistry databases

DrugBankiDB03615. Ribostamycin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004168291 – 145Aminoglycoside N(6')-acetyltransferase type 1Add BLAST145

Interactioni

Subunit structurei

Homodimer.2 Publications

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Structurei

Secondary structure

1145
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 5Combined sources5
Helixi8 – 10Combined sources3
Helixi11 – 21Combined sources11
Helixi27 – 39Combined sources13
Beta strandi41 – 50Combined sources10
Beta strandi53 – 63Combined sources11
Beta strandi71 – 83Combined sources13
Helixi85 – 87Combined sources3
Beta strandi89 – 91Combined sources3
Helixi92 – 106Combined sources15
Beta strandi110 – 116Combined sources7
Helixi121 – 129Combined sources9
Beta strandi133 – 144Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S3ZX-ray2.00A/B1-145[»]
1S5KX-ray2.40A/B1-145[»]
1S60X-ray3.00A1-145[»]
2VBQX-ray2.00A/B1-145[»]
ProteinModelPortaliQ9R381.
SMRiQ9R381.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9R381.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 145N-acetyltransferasePROSITE-ProRule annotationAdd BLAST145

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni81 – 83Acetyl-CoA binding2 Publications3
Regioni89 – 94Acetyl-CoA binding2 Publications6

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK18816.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR024170. Aminoglycoside_N6-AcTrfrase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000452. 6-N-acetyltransf. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R381-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIRQMNKTH LEHWRGLRKQ LWPGHPDDAH LADGEEILQA DHLASFIAMA
60 70 80 90 100
DGVAIGFADA SIRHDYVNGC DSSPVVFLEG IFVLPSFRQR GVAKQLIAAV
110 120 130 140
QRWGTNKGCR EMASDTSPEN TISQKVHQAL GFEETERVIF YRKRC
Length:145
Mass (Da):16,362
Last modified:May 1, 2000 - v1
Checksum:i9CD8BBFD48BC43A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF144880 Genomic DNA. Translation: AAF03531.1.
AF144881 Genomic DNA. Translation: AAF03532.1.
RefSeqiWP_000354853.1. NZ_JYXI01000003.1.

Genome annotation databases

KEGGiag:AAF03531.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF144880 Genomic DNA. Translation: AAF03531.1.
AF144881 Genomic DNA. Translation: AAF03532.1.
RefSeqiWP_000354853.1. NZ_JYXI01000003.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S3ZX-ray2.00A/B1-145[»]
1S5KX-ray2.40A/B1-145[»]
1S60X-ray3.00A1-145[»]
2VBQX-ray2.00A/B1-145[»]
ProteinModelPortaliQ9R381.
SMRiQ9R381.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

DrugBankiDB03615. Ribostamycin.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAF03531.

Phylogenomic databases

KOiK18816.

Miscellaneous databases

EvolutionaryTraceiQ9R381.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR024170. Aminoglycoside_N6-AcTrfrase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000452. 6-N-acetyltransf. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAAC6_SALEN
AccessioniPrimary (citable) accession number: Q9R381
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Strain BM4361 does not express or weakly expresses aminoglycoside resistance gene and is thus aminoglycoside-sensitive. Strain BM4362 expresses it due to a chromosomal deletion leading to aminoglycoside resistance.1 Publication

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.