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Protein

Alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3-N-acetyl-galactosaminide alpha-2,6-sialyltransferase

Gene

St6galnac4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of ganglioside GD1A from GM1B. Transfers CMP-NeuAc with an alpha-2,6-linkage to GalNAc residue on NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc of glycoproteins and glycolipids. Prefers glycoproteins to glycolipids.

Catalytic activityi

CMP-N-acetylneuraminate + N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-D-galactosaminyl-R = CMP + N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-(N-acetyl-alpha-neuraminyl-(2->6))-N-acetyl-D-galactosaminyl-R.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Chemistry

SwissLipidsiSLP:000000785.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3-N-acetyl-galactosaminide alpha-2,6-sialyltransferase (EC:2.4.99.7)
Alternative name(s):
NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc-alpha-2,6-sialyltransferase
ST6GalNAc IV
Short name:
ST6GalNAcIV
Sialyltransferase 7D
Short name:
SIAT7-D
Gene namesi
Name:St6galnac4
Synonyms:Siat7d
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1341894. St6galnac4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7171CytoplasmicSequence analysisAdd
BLAST
Transmembranei72 – 9423Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini95 – 360266LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360Alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3-N-acetyl-galactosaminide alpha-2,6-sialyltransferasePRO_0000149279Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi134 ↔ 283By similarity
Glycosylationi193 – 1931N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9R2B6.
PaxDbiQ9R2B6.
PRIDEiQ9R2B6.

PTM databases

iPTMnetiQ9R2B6.
PhosphoSiteiQ9R2B6.

Expressioni

Tissue specificityi

High expression in brain and colon and to a lesser extent in lung, heart, kidney, spleen and thymus.1 Publication

Developmental stagei

Developmentally regulated.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099882.

Structurei

3D structure databases

ProteinModelPortaliQ9R2B6.
SMRiQ9R2B6. Positions 118-324.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
HOGENOMiHOG000293316.
HOVERGENiHBG058710.
InParanoidiQ9R2B6.
KOiK03374.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Experimental confirmation may be lacking for some isoforms.

Isoform 1 (identifier: Q9R2B6-1) [UniParc]FASTAAdd to basket

Also known as: Long 2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEHVVTCWRL KLLSWPVFLI WICLSLASVS LISWDQLPAF LIPSTGDSSL
60 70 80 90 100
QTAKSRDSMK APGRLLLLTL CILTFSAVCV FLCCWACLPL CLATCLDRHL
110 120 130 140 150
PAAPRSTVPG PLHFSGYSSV PDGKPLIREL CHSCAVVSNS GQMLGSGLGA
160 170 180 190 200
QIDGAECVLR MNQAPTVGFE EDVGQRTTLR VISHTSVPLL LRNYSHYFQH
210 220 230 240 250
ARDTLYVVWG QGRHMDRVLG GRTYRTLLQL TRMYPGLQVY TFTERMMAYC
260 270 280 290 300
DQIFQDETGK NRRQSGSFLS TGWFTMIPAL ELCEEIVVYG MVSDSYCSEK
310 320 330 340 350
SPRSVPYHYF EKGRLDECQM YRLHEQAPRS AHRFITEKAV FSRWAKKRPI
360
VFAHPSWRAK
Length:360
Mass (Da):40,773
Last modified:May 1, 2000 - v1
Checksum:i7CF4101B8FDA369A
GO
Isoform 2 (identifier: Q9R2B6-2) [UniParc]FASTAAdd to basket

Also known as: Long 1

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: MEHVVTCWRLKLLSWPVFLIWICLSLAS → MSSEQRILSPQRTPIRSF

Show »
Length:350
Mass (Da):39,562
Checksum:i30537BE72B636786
GO
Isoform 3 (identifier: Q9R2B6-3) [UniParc]FASTAAdd to basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     1-58: Missing.

Show »
Length:302
Mass (Da):34,244
Checksum:i39A3E973F2D80EA6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5858Missing in isoform 3. CuratedVSP_001787Add
BLAST
Alternative sequencei1 – 2828MEHVV…LSLAS → MSSEQRILSPQRTPIRSF in isoform 2. CuratedVSP_001788Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15780 mRNA. Translation: CAB43514.1.
Y15780 mRNA. Translation: CAB43515.1.
Y15779 mRNA. Translation: CAB43507.1.
Y15779 mRNA. Translation: CAB43508.1.
AJ007310 mRNA. Translation: CAA07446.1.
Y19057 Genomic DNA. Translation: CAB93948.1.
CCDSiCCDS15921.1. [Q9R2B6-3]
RefSeqiNP_001263354.1. NM_001276425.1.
NP_035503.1. NM_011373.3.
UniGeneiMm.27446.

Genome annotation databases

GeneIDi20448.
KEGGimmu:20448.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST6GalNAc IV

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15780 mRNA. Translation: CAB43514.1.
Y15780 mRNA. Translation: CAB43515.1.
Y15779 mRNA. Translation: CAB43507.1.
Y15779 mRNA. Translation: CAB43508.1.
AJ007310 mRNA. Translation: CAA07446.1.
Y19057 Genomic DNA. Translation: CAB93948.1.
CCDSiCCDS15921.1. [Q9R2B6-3]
RefSeqiNP_001263354.1. NM_001276425.1.
NP_035503.1. NM_011373.3.
UniGeneiMm.27446.

3D structure databases

ProteinModelPortaliQ9R2B6.
SMRiQ9R2B6. Positions 118-324.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099882.

Chemistry

SwissLipidsiSLP:000000785.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

iPTMnetiQ9R2B6.
PhosphoSiteiQ9R2B6.

Proteomic databases

MaxQBiQ9R2B6.
PaxDbiQ9R2B6.
PRIDEiQ9R2B6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20448.
KEGGimmu:20448.

Organism-specific databases

CTDi27090.
MGIiMGI:1341894. St6galnac4.

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
HOGENOMiHOG000293316.
HOVERGENiHBG058710.
InParanoidiQ9R2B6.
KOiK03374.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

ChiTaRSiSt6galnac4. mouse.
PROiQ9R2B6.
SOURCEiSearch...

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and functional expression of two members of mouse NeuAc-alpha-2,3Gal-beta-1,3GalNAc GalNAc-alpha2,6-sialyltransferase family, ST6GalNAc III and IV."
    Lee Y.-C., Kaufman M., Kitazume-Kawaguchi S., Kono M., Takashima S., Kurosawa N., Liu H., Pircher H., Tsuji S.
    J. Biol. Chem. 274:11958-11967(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, TISSUE SPECIFICITY, ALTERNATIVE SPLICING, DEVELOPMENTAL STAGE.
    Strain: ICR.
    Tissue: Brain.
  2. "Comparative analysis of the genomic structures and promoter activities of mouse Siaa2,3Galb1,3GalNAc GalNAca2,6-sialyltransferase genes (ST6GalNAc III and IV): characterization of their Sp1 binding."
    Takashima S., Kurosawa N., Tachida Y., Inoue M., Tsuji S.
    J. Biochem. 127:399-409(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 299-360.
    Strain: C57BL/6J.

Entry informationi

Entry nameiSIA7D_MOUSE
AccessioniPrimary (citable) accession number: Q9R2B6
Secondary accession number(s): O88725
, Q9JHP0, Q9QUP9, Q9R2B5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.