Q9R283 (TRPC2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Short transient receptor potential channel 2
Short name=TrpC2

Alternative name(s):
Transient receptor protein 2
Short name=TRP-2
Short name=rTrp2

Gene names

Name:	Trpc2
Synonyms:	Trp2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1170 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Thought to form a receptor-activated calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Is not activated by intracellular calcium store depletion.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Tissue specificity	Expressed exclusively in vomeronasal organ neurons (sensory microvilli).
Sequence similarities	Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC2 sub-subfamily. [View classification] Contains 4 ANK repeats.

Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	ANK repeat Coiled coil Repeat Transmembrane Transmembrane helix
Ligand	Calcium
Molecular function	Calcium channel Ion channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	single strand break repair Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: InterPro
Molecular_function	calcium channel activity Inferred from electronic annotation. Source: UniProtKB-KW damaged DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9R283-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available. Inferred from mouse sequence.

Isoform 2 (identifier: Q9R283-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-283: Missing.
     284-288: PLIPK → MDPLS

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9R283-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAPVKISHVV...PFVTLLPATM
     274-315: RCLHVALTPV...LRAIQEGQLG → WVSSPESSPP...ASVCGEVFPA
     316-1170: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1170	1170	Short transient receptor potential channel 2		PRO_0000215308
Regions
	Topological domain	1 – 627	627	Cytoplasmic Potential
	Transmembrane	628 – 648	21	Helical; Potential
	Topological domain	649 – 658	10	Extracellular Potential
	Transmembrane	659 – 679	21	Helical; Potential
	Topological domain	680 – 701	22	Cytoplasmic Potential
	Transmembrane	702 – 722	21	Helical; Potential
	Topological domain	723 – 737	15	Extracellular Potential
	Transmembrane	738 – 758	21	Helical; Potential
	Topological domain	759 – 788	30	Cytoplasmic Potential
	Transmembrane	789 – 809	21	Helical; Potential
	Topological domain	810 – 832	23	Extracellular Potential
	Transmembrane	833 – 853	21	Helical; Potential
	Topological domain	854 – 898	45	Cytoplasmic Potential
	Transmembrane	899 – 919	21	Helical; Potential
	Topological domain	920 – 1170	251	Extracellular Potential
	Repeat	300 – 329	30	ANK 1
	Repeat	346 – 376	31	ANK 2
	Repeat	377 – 405	29	ANK 3
	Repeat	429 – 458	30	ANK 4
	Coiled coil	1030 – 1068	39	Potential
	Compositional bias	197 – 201	5	Poly-Ser
	Compositional bias	1136 – 1141	6	Poly-Ser
Natural variations
	Alternative sequence	1 – 283	283	Missing in isoform 2.		VSP_037236
	Alternative sequence	1	1	M → MAPVKISHVVSFSSQDPKYP VENLLNPDSHKGPWLSCPRD KTGQLKVEFQLERAVPISYI DVGNCGCAFLQIDVGRSSWP LDRPFVTLLPATM in isoform 3.		VSP_038807
	Alternative sequence	274 – 315	42	RCLHV…EGQLG → WVSSPESSPPPSSWVQCPIC ELQFSAREIEEHASVCGEVF PA in isoform 3.		VSP_037238
	Alternative sequence	284 – 288	5	PLIPK → MDPLS in isoform 2.		VSP_037237
	Alternative sequence	316 – 1170	855	Missing in isoform 3.		VSP_037239
Experimental info
	Sequence conflict	1010	1	H → R in AAD31453. Ref.1
	Sequence conflict	1069 – 1070	2	Missing in AAD31453. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: BDF876A83B28CA5C	FASTA	1,170	130,467
	10 20 30 40 50 60 MLMSLTDSKE GKNRSGVRMF KDDDFLIPAS GESWDRLRLT CSQPFTRHQS FGLAFLRVRS 70 80 90 100 110 120 SLDSLSDPVK DPSSPGSSGL NQNSSDKLES DPSPWLTNPS IRRTFFPDPQ TSTKEISALK 130 140 150 160 170 180 GMLKQLQPGP LGRAARMVLS AAHKAPPASV VSPNNSHGEP DSSHPERAEP RAEEPNRKNN 190 200 210 220 230 240 ASRGKRRKVQ EQRRPLSSSS SQPNRRATGR TKQRQQRPQA KSDGSGVQAT GQCPICTGSF 250 260 270 280 290 300 SIEALPRHAA TCGESSPPQP ASPTSLSSSE SVLRCLHVAL TPVPLIPKPN WTEIVNKKLK 310 320 330 340 350 360 FPPTLLRAIQ EGQLGLVQQL LESGSDPSGA GPGGPLRNVE ESEDRSWREA LNLAIRLGHE 370 380 390 400 410 420 VITDVLLANV KFDFRQIHEA LLVAVDTNQP AVVRRLLARL EREKGRKVDT KSFSLAFFDS 430 440 450 460 470 480 SIDGSRFAPG VTPLTLACQK DLYEIAQLLM DQGHTIARPH PVSCACLECS NARRYDLLKF 490 500 510 520 530 540 SLSRINTYRG IASRAHLSLA SEDAMLAAFQ LSRELRRLAR KEPEFKPQYI ALESLCQDYG 550 560 570 580 590 600 FELLGMCRNQ SEVTAVLNDL GEDSETEPEA EGLGQAFEEG IPNLARLRLA VNYNQKQFVA 610 620 630 640 650 660 HPICQQVLSS IWCGNLAGWR GSTTIWKLFV AFLIFLTMPF LCIGYWLAPK SRLGRLLKIP 670 680 690 700 710 720 VLKFLLHSAS YLWFLIFLLG ESLVMETQLS TFKGRSQSVW ETSLHMIWVT GFLWFECKEV 730 740 750 760 770 780 WIEGLRSYLL DWWNFLDVVI LSLYLASFAL RLLLAGLAYM HCRDASDSST CRYFTTAERS 790 800 810 820 830 840 EWRTEDPQFL AEVLFAVTSM LSFTRLAYIL PAHESLGTLQ ISIGKMIDDM IRFMFILMII 850 860 870 880 890 900 LTAFLCGLNN IYVPYQETEK LGNFNETFQF LFWTMFGMEE HSVVDMPQFL VPEFVGRAMY 910 920 930 940 950 960 GIFTIVMVIV LLNMLIAMIT NSFQKIEDDA DVEWKFARSK LYLSYFREGL TLPVPFNILP 970 980 990 1000 1010 1020 SPKAAFYLLR RIFRFICCGS SCCKAKKSDY PPIPTFTNPG ARAGPGEGEH VSYRLRVIKA 1030 1040 1050 1060 1070 1080 LVQRYIETAR REFEETRRKD LGNRLTELTK TVSRLQSEVA SVQKTVAAGG ALRPPDGASI 1090 1100 1110 1120 1130 1140 LSRYITRVRN SFQNLGPPAP DTPAELTMPG IVETEVSLED SLDATGEAGT PASGESSSSS 1150 1160 1170 SAHVLVHREQ EAEGAGDLPL GEDLETKGES
	Isoform 2 [UniParc]. Checksum: F8A516AEB39F3A50	FASTA	887	99,809
	10 20 30 40 50 60 MDPLSPNWTE IVNKKLKFPP TLLRAIQEGQ LGLVQQLLES GSDPSGAGPG GPLRNVEESE 70 80 90 100 110 120 DRSWREALNL AIRLGHEVIT DVLLANVKFD FRQIHEALLV AVDTNQPAVV RRLLARLERE 130 140 150 160 170 180 KGRKVDTKSF SLAFFDSSID GSRFAPGVTP LTLACQKDLY EIAQLLMDQG HTIARPHPVS 190 200 210 220 230 240 CACLECSNAR RYDLLKFSLS RINTYRGIAS RAHLSLASED AMLAAFQLSR ELRRLARKEP 250 260 270 280 290 300 EFKPQYIALE SLCQDYGFEL LGMCRNQSEV TAVLNDLGED SETEPEAEGL GQAFEEGIPN 310 320 330 340 350 360 LARLRLAVNY NQKQFVAHPI CQQVLSSIWC GNLAGWRGST TIWKLFVAFL IFLTMPFLCI 370 380 390 400 410 420 GYWLAPKSRL GRLLKIPVLK FLLHSASYLW FLIFLLGESL VMETQLSTFK GRSQSVWETS 430 440 450 460 470 480 LHMIWVTGFL WFECKEVWIE GLRSYLLDWW NFLDVVILSL YLASFALRLL LAGLAYMHCR 490 500 510 520 530 540 DASDSSTCRY FTTAERSEWR TEDPQFLAEV LFAVTSMLSF TRLAYILPAH ESLGTLQISI 550 560 570 580 590 600 GKMIDDMIRF MFILMIILTA FLCGLNNIYV PYQETEKLGN FNETFQFLFW TMFGMEEHSV 610 620 630 640 650 660 VDMPQFLVPE FVGRAMYGIF TIVMVIVLLN MLIAMITNSF QKIEDDADVE WKFARSKLYL 670 680 690 700 710 720 SYFREGLTLP VPFNILPSPK AAFYLLRRIF RFICCGSSCC KAKKSDYPPI PTFTNPGARA 730 740 750 760 770 780 GPGEGEHVSY RLRVIKALVQ RYIETARREF EETRRKDLGN RLTELTKTVS RLQSEVASVQ 790 800 810 820 830 840 KTVAAGGALR PPDGASILSR YITRVRNSFQ NLGPPAPDTP AELTMPGIVE TEVSLEDSLD 850 860 870 880 ATGEAGTPAS GESSSSSSAH VLVHREQEAE GAGDLPLGED LETKGES
	Isoform 3 [UniParc]. Checksum: 3C1FAF331F007681	FASTA	407	44,359
	10 20 30 40 50 60 MAPVKISHVV SFSSQDPKYP VENLLNPDSH KGPWLSCPRD KTGQLKVEFQ LERAVPISYI 70 80 90 100 110 120 DVGNCGCAFL QIDVGRSSWP LDRPFVTLLP ATMLMSLTDS KEGKNRSGVR MFKDDDFLIP 130 140 150 160 170 180 ASGESWDRLR LTCSQPFTRH QSFGLAFLRV RSSLDSLSDP VKDPSSPGSS GLNQNSSDKL 190 200 210 220 230 240 ESDPSPWLTN PSIRRTFFPD PQTSTKEISA LKGMLKQLQP GPLGRAARMV LSAAHKAPPA 250 260 270 280 290 300 SVVSPNNSHG EPDSSHPERA EPRAEEPNRK NNASRGKRRK VQEQRRPLSS SSSQPNRRAT 310 320 330 340 350 360 GRTKQRQQRP QAKSDGSGVQ ATGQCPICTG SFSIEALPRH AATCGESSPP QPASPTSLSS 370 380 390 400 SESVLWVSSP ESSPPPSSWV QCPICELQFS AREIEEHASV CGEVFPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TRP2: a candidate transduction channel for mammalian pheromone sensory signaling." Liman E.R., Corey D.P., Dulac C. Proc. Natl. Acad. Sci. U.S.A. 96:5791-5796(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S. Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Embryo.
[4]	"Expression patterns of mammalian trp homologues in rat excitable cells." Koizumi S., Ripley S.J., Bootman M.D., Lipp P., Berridge M.J., Bobanovic L.K. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 867-930 (ISOFORM 1/2). Strain: Wistar. Tissue: Heart.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF136401 mRNA. Translation: AAD31453.1. AABR03002379 Genomic DNA. No translation available. AABR03010502 Genomic DNA. No translation available. AABR03010734 Genomic DNA. No translation available. AABR03001253 Genomic DNA. No translation available. BC169022 mRNA. Translation: AAI69022.1. AF061874 mRNA. Translation: AAC16726.1.
IPI	IPI00569206. IPI00914168. IPI00948958.
UniGene	Rn.48766.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000027391.
Proteomic databases
PaxDb	Q9R283.
PRIDE	Q9R283.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000027358; ENSRNOP00000027358; ENSRNOG00000020188. ENSRNOT00000045726; ENSRNOP00000042378; ENSRNOG00000020188. ENSRNOT00000048172; ENSRNOP00000047354; ENSRNOG00000020188.
UCSC	RGD:628819. rat.
Organism-specific databases
RGD	628819. Trpc2.
Phylogenomic databases
eggNOG	NOG257288.
GeneTree	ENSGT00680000099764.
HOGENOM	HOG000137678.
HOVERGEN	HBG061894.
InParanoid	O70485.
OMA	SEWRTED.
OrthoDB	EOG49CQ72.
Gene expression databases
ArrayExpress	Q9R283.
Genevestigator	Q9R283.
GermOnline	ENSRNOG00000020188. Rattus norvegicus.
Family and domain databases
Gene3D	1.25.40.20. 1 hit.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR008979. Galactose-bd-like. IPR005821. Ion_trans_dom. IPR013555. TRP_dom. IPR005458. TRPC2_channel. IPR002153. TRPC_channel. IPR002706. Xrcc1_N. [Graphical view]
PANTHER	PTHR10117. PTHR10117. 1 hit. PTHR10117:SF6. PTHR10117:SF6. 1 hit.
Pfam	PF12796. Ank_2. 1 hit. PF00520. Ion_trans. 1 hit. PF08344. TRP_2. 1 hit. PF01834. XRCC1_N. 1 hit. [Graphical view]
PRINTS	PR01097. TRNSRECEPTRP. PR01643. TRPCHANNEL2.
SMART	SM00248. ANK. 3 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit. SSF49785. Gal_bind_like. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. False negative. PS50088. ANK_REPEAT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPC2_RAT

Accession

Primary (citable) accession number: Q9R283
Secondary accession number(s): B5DFD7, O70485

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	May 5, 2009
Last modified:	April 3, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program