ID   TRPC2_MOUSE             Reviewed;        1172 AA.
AC   Q9R244; Q9ES59; Q9ES60; Q9R243;
DT   20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   23-MAR-2010, sequence version 2.
DT   24-JAN-2024, entry version 183.
DE   RecName: Full=Short transient receptor potential channel 2;
DE            Short=TrpC2;
DE   AltName: Full=Transient receptor protein 2;
DE            Short=TRP-2;
DE            Short=mTrp2;
GN   Name=Trpc2; Synonyms=Trp2, Trrp2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Testis;
RX   PubMed=10051594; DOI=10.1073/pnas.96.5.2060;
RA   Vannier B., Peyton M., Boulay G., Brown D., Qin N., Jiang M., Zhu X.,
RA   Birnbaumer L.;
RT   "Mouse trp2, the homologue of the human trpc2 pseudogene, encodes mTrp2, a
RT   store depletion-activated capacitative Ca2+ entry channel.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:2060-2064(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4).
RX   PubMed=10998353; DOI=10.1042/0264-6021:3510115;
RA   Hofmann T., Schaefer M., Schultz G., Gudermann T.;
RT   "Cloning, expression and subcellular localization of two novel splice
RT   variants of mouse transient receptor potential channel 2.";
RL   Biochem. J. 351:115-122(2000).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=11972034; DOI=10.1073/pnas.082127599;
RA   Leypold B.G., Yu C.R., Leinders-Zufall T., Kim M.M., Zufall F., Axel R.;
RT   "Altered sexual and social behaviors in trp2 mutant mice.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:6376-6381(2002).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=11823606; DOI=10.1126/science.1069259;
RA   Stowers L., Holy T.E., Meister M., Dulac C., Koentges G.;
RT   "Loss of sex discrimination and male-male aggression in mice deficient for
RT   TRP2.";
RL   Science 295:1493-1500(2002).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=20596023; DOI=10.1038/nature09142;
RA   Haga S., Hattori T., Sato T., Sato K., Matsuda S., Kobayakawa R.,
RA   Sakano H., Yoshihara Y., Kikusui T., Touhara K.;
RT   "The male mouse pheromone ESP1 enhances female sexual receptive behaviour
RT   through a specific vomeronasal receptor.";
RL   Nature 466:118-122(2010).
RN   [6]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=24089208; DOI=10.1038/nature12579;
RA   Ferrero D.M., Moeller L.M., Osakada T., Horio N., Li Q., Roy D.S.,
RA   Cichy A., Spehr M., Touhara K., Liberles S.D.;
RT   "A juvenile mouse pheromone inhibits sexual behaviour through the
RT   vomeronasal system.";
RL   Nature 502:368-371(2013).
CC   -!- FUNCTION: Thought to form a receptor-activated non-selective calcium
CC       permeant cation channel. Probably is operated by a phosphatidylinositol
CC       second messenger system activated by receptor tyrosine kinases or G-
CC       protein coupled receptors. May also be activated by intracellular
CC       calcium store depletion. Plays a role in mediating responsivity to
CC       pheromones that elicit aggressive and mating behaviors. Required for
CC       response to the Esp1 pheromone which enhances female sexual receptive
CC       behavior and to the Esp22 pheromone which inhibits adult male mating
CC       behavior. {ECO:0000269|PubMed:11823606, ECO:0000269|PubMed:11972034,
CC       ECO:0000269|PubMed:20596023, ECO:0000269|PubMed:24089208}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=4;
CC       Name=1; Synonyms=A;
CC         IsoId=Q9R244-1; Sequence=Displayed;
CC       Name=2; Synonyms=B;
CC         IsoId=Q9R244-2; Sequence=VSP_006562;
CC       Name=3; Synonyms=Alpha;
CC         IsoId=Q9R244-3; Sequence=VSP_006564;
CC       Name=4; Synonyms=Beta;
CC         IsoId=Q9R244-4; Sequence=VSP_006563, VSP_006565;
CC   -!- TISSUE SPECIFICITY: Isoform 3 is ubiquitously expressed at low levels.
CC       Isoform 4 is expressed exclusively in vomeronasal organ.
CC   -!- DISRUPTION PHENOTYPE: Reduced electrophysiological response to
CC       pheromone mixtures in the vomeronasal organ. Mutant males and nursing
CC       females are docile and fail to initiate aggressive attacks on intruder
CC       males. Males display normal mating behavior towards females but display
CC       increased sexual behavior towards prepubescent females even when
CC       presented simultaneously with adult estrus females and also show
CC       increased sexual behavior towards juvenile and adult males. Lack of
CC       response to Esp1. {ECO:0000269|PubMed:11823606,
CC       ECO:0000269|PubMed:11972034, ECO:0000269|PubMed:20596023,
CC       ECO:0000269|PubMed:24089208}.
CC   -!- SIMILARITY: Belongs to the transient receptor (TC 1.A.4) family. STrpC
CC       subfamily. TRPC2 sub-subfamily. {ECO:0000305}.
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DR   EMBL; AF111108; AAD17196.1; -; mRNA.
DR   EMBL; AF111107; AAD17195.1; -; mRNA.
DR   EMBL; AF230803; AAG29951.1; -; mRNA.
DR   EMBL; AF230802; AAG29950.1; -; mRNA.
DR   CCDS; CCDS52333.1; -. [Q9R244-4]
DR   RefSeq; NP_001103367.1; NM_001109897.2. [Q9R244-4]
DR   AlphaFoldDB; Q9R244; -.
DR   SMR; Q9R244; -.
DR   BioGRID; 204328; 1.
DR   CORUM; Q9R244; -.
DR   DIP; DIP-61090N; -.
DR   IntAct; Q9R244; 2.
DR   STRING; 10090.ENSMUSP00000081903; -.
DR   iPTMnet; Q9R244; -.
DR   PhosphoSitePlus; Q9R244; -.
DR   PaxDb; 10090-ENSMUSP00000081903; -.
DR   DNASU; 22064; -.
DR   Ensembl; ENSMUST00000124189.3; ENSMUSP00000116934.3; ENSMUSG00000100254.2. [Q9R244-4]
DR   GeneID; 22064; -.
DR   KEGG; mmu:22064; -.
DR   UCSC; uc009iqm.2; mouse. [Q9R244-3]
DR   UCSC; uc009iqn.2; mouse. [Q9R244-2]
DR   UCSC; uc012fqr.2; mouse. [Q9R244-1]
DR   UCSC; uc012fqs.2; mouse. [Q9R244-4]
DR   AGR; MGI:109527; -.
DR   CTD; 7221; -.
DR   MGI; MGI:109527; Trpc2.
DR   VEuPathDB; HostDB:ENSMUSG00000100254; -.
DR   eggNOG; KOG3609; Eukaryota.
DR   HOGENOM; CLU_005716_2_0_1; -.
DR   InParanoid; Q9R244; -.
DR   OMA; MIDDMMF; -.
DR   OrthoDB; 5406916at2759; -.
DR   PhylomeDB; Q9R244; -.
DR   BioGRID-ORCS; 22064; 2 hits in 80 CRISPR screens.
DR   ChiTaRS; Dct; mouse.
DR   PRO; PR:Q9R244; -.
DR   Proteomes; UP000000589; Chromosome 7.
DR   RNAct; Q9R244; Protein.
DR   Bgee; ENSMUSG00000100254; Expressed in mesentery of urinary system and 45 other cell types or tissues.
DR   GO; GO:0034703; C:cation channel complex; IBA:GO_Central.
DR   GO; GO:0032590; C:dendrite membrane; IMP:MGI.
DR   GO; GO:0012505; C:endomembrane system; IDA:MGI.
DR   GO; GO:0005634; C:nucleus; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR   GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR   GO; GO:0019992; F:diacylglycerol binding; IMP:MGI.
DR   GO; GO:0070679; F:inositol 1,4,5 trisphosphate binding; IDA:BHF-UCL.
DR   GO; GO:0015279; F:store-operated calcium channel activity; IDA:MGI.
DR   GO; GO:0007340; P:acrosome reaction; IMP:MGI.
DR   GO; GO:0002118; P:aggressive behavior; IMP:MGI.
DR   GO; GO:0070588; P:calcium ion transmembrane transport; IBA:GO_Central.
DR   GO; GO:0006816; P:calcium ion transport; IMP:MGI.
DR   GO; GO:0008050; P:female courtship behavior; IMP:MGI.
DR   GO; GO:0002121; P:inter-male aggressive behavior; IMP:MGI.
DR   GO; GO:0048047; P:mating behavior, sex discrimination; IMP:MGI.
DR   GO; GO:1902436; P:negative regulation of male mating behavior; IGI:MGI.
DR   GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IPI:MGI.
DR   GO; GO:0051480; P:regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR   GO; GO:0010468; P:regulation of gene expression; IMP:MGI.
DR   GO; GO:0019236; P:response to pheromone; IMP:MGI.
DR   GO; GO:0007338; P:single fertilization; IBA:GO_Central.
DR   GO; GO:0000012; P:single strand break repair; IEA:InterPro.
DR   GO; GO:0002124; P:territorial aggressive behavior; IMP:MGI.
DR   Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 1.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR005821; Ion_trans_dom.
DR   InterPro; IPR013555; TRP_dom.
DR   InterPro; IPR005458; TRPC2_channel.
DR   InterPro; IPR002153; TRPC_channel.
DR   InterPro; IPR002706; Xrcc1_N.
DR   PANTHER; PTHR10117:SF6; SHORT TRANSIENT RECEPTOR POTENTIAL CHANNEL 2; 1.
DR   PANTHER; PTHR10117; TRANSIENT RECEPTOR POTENTIAL CHANNEL; 1.
DR   Pfam; PF00520; Ion_trans; 1.
DR   Pfam; PF08344; TRP_2; 1.
DR   Pfam; PF01834; XRCC1_N; 1.
DR   PRINTS; PR01097; TRNSRECEPTRP.
DR   PRINTS; PR01643; TRPCHANNEL2.
DR   SMART; SM01420; TRP_2; 1.
DR   SUPFAM; SSF48403; Ankyrin repeat; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR   Genevisible; Q9R244; MM.
PE   2: Evidence at transcript level;
KW   Alternative splicing; ANK repeat; Calcium; Calcium channel;
KW   Calcium transport; Ion channel; Ion transport; Membrane;
KW   Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..1172
FT                   /note="Short transient receptor potential channel 2"
FT                   /id="PRO_0000215307"
FT   TOPO_DOM        1..659
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        660..680
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        681..702
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        703..723
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        724..738
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        739..759
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        760..789
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        790..810
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        811..833
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        834..854
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        855..899
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        900..920
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        921..1172
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REPEAT          301..330
FT                   /note="ANK 1"
FT   REPEAT          377..406
FT                   /note="ANK 2"
FT   REPEAT          430..459
FT                   /note="ANK 3"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          69..98
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          140..227
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          249..271
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1118..1172
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        75..91
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        164..180
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1148..1172
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         1..111
FT                   /note="MLMSRTDSKSGKNRSGVRMFKDGDFLTPASGESWDRLRLTCSQPFTRHQSFG
FT                   LAFLRVRSSLGSLADPVVDPSAPGSSGLNQNSTDVLESDPRPWLTNPSIRRTFFPDPQT
FT                   -> MGTKTHPVVPW (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:10051594"
FT                   /id="VSP_006562"
FT   VAR_SEQ         2..287
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:10998353"
FT                   /id="VSP_006564"
FT   VAR_SEQ         2..283
FT                   /note="Missing (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:10998353"
FT                   /id="VSP_006563"
FT   VAR_SEQ         284..287
FT                   /note="LVPK -> DPLS (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:10998353"
FT                   /id="VSP_006565"
FT   CONFLICT        628
FT                   /note="K -> R (in Ref. 1; AAD17195/AAD17196)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        633
FT                   /note="F -> S (in Ref. 1; AAD17195/AAD17196)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        653
FT                   /note="Q -> R (in Ref. 2; AAG29951/AAG29950)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        774
FT                   /note="Y -> C (in Ref. 1; AAD17195/AAD17196)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        797
FT                   /note="A -> T (in Ref. 1; AAD17195/AAD17196)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1161
FT                   /note="L -> P (in Ref. 2; AAG29951/AAG29950)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1172 AA;  130531 MW;  D9A9A9FEE01D1536 CRC64;
     MLMSRTDSKS GKNRSGVRMF KDGDFLTPAS GESWDRLRLT CSQPFTRHQS FGLAFLRVRS
     SLGSLADPVV DPSAPGSSGL NQNSTDVLES DPRPWLTNPS IRRTFFPDPQ TSTKEISELK
     GMLKQLQPGP LGRAARMVLS AARKAPPASV VSPNNSHGEP GPSRAESAEP RAEEPNRKTA
     VGRRKRRKVQ EPRRSLSNSS SQPNRRTGRT RQRQHRPQTK SDDGGVQAAG QCPICAGFFS
     IETLPQHAAT CGESPPPQPA SPASLSSSES VLRRHHVALT PVPLVPKPQP NWTEIVNKKL
     KFPPTLLRAI QEGQLGLVQQ LLESSSDASG AGPGGPLRNV EESEDRSWRE ALNLAIRLGH
     EVITDVLLAN VKFDFRQIHE ALLVAVDTNQ PAVVRRLLAR LEREKGRKVD TKSFSLAFFD
     SSIDGSRFAP GVTPLTLACQ KDLYEIAQLL MDQGHTIARP HPVSCACLEC SNARRYDLLK
     FSLSRINTYR GIASRAHLSL ASEDAMLAAF QLSRELRRLA RKEPEFKPQY IALESLCQDY
     GFELLGMCRN QSEVTAVLND LGEDSETEPE AEGLGQAFEE GIPNLARLRL AVNYNQKQFV
     AHPICQQVLS SIWCGNLAGW RGSTTIWKLF VAFLIFLTMP FLCIGYWLAP KSQLGRLLKI
     PVLKFLLHSA SYLWFLIFLL GESLVMETQL STFKGRSQSV WETSLHMIWV TGFLWFECKE
     VWIEGLRSYL LDWWNFLDVV ILSLYLASFA LRLLLAGLAY MHCRDASDST TCRYFTTAER
     SEWRTEDPQF LAEVLFAVTS MLSFTRLAYI LPAHESLGTL QISIGKMIDD MIRFMFILMI
     ILTAFLCGLN NIYVPYQESE KLGNFNETFQ FLFWTMFGME EHTVVDMPQF LVPEFVGRAM
     YGIFTIVMVI VLLNMLIAMI TNSFQKIEDD ADVEWKFARS KLYLSYFREG LTLPVPFNIL
     PSPKAAFYLV RRIFRFLCCG SSCCKAKKSD YPPIGTFTNP GARAGSAGEG ERVSYRLRVI
     KALVQRYIET ARREFEETRR KDLGNRLTEL TKTVSRLQSE VASVQKNLAA GGAPRPPDGA
     SILSRYITRV RNSFQNLGPP TSDTPAELTM PGIVETEVSL GDGLDGTGEA GAPAPGEPGS
     SSSAHVLVHR EQEAEGSGDL LLEGDLETKG ES
//