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Q9R244 (TRPC2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Short transient receptor potential channel 2

Short name=TrpC2
Alternative name(s):
Transient receptor protein 2
Short name=TRP-2
Short name=mTrp2
Gene names
Name:Trpc2
Synonyms:Trp2, Trrp2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1172 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. May also be activated by intracellular calcium store depletion. Plays a role in mediating responsivity to pheromones that elicit aggressive and mating behaviors. Required for response to the Esp1 pheromone which enhances female sexual receptive behavior and to the Esp22 pheromone which inhibits adult male mating behavior. Ref.3 Ref.4 Ref.5 Ref.6

Subcellular location

Membrane; Multi-pass membrane protein Probable.

Tissue specificity

Isoform 3 is ubiquitously expressed at low levels. Isoform 4 is expressed exclusively in vomeronasal organ.

Disruption phenotype

Reduced electrophysiological response to pheromone mixtures in the vomeronasal organ. Mutant males and nursing females are docile and fail to initiate aggressive attacks on intruder males. Males display normal mating behavior towards females but display increased sexual behavior towards prepubescent females even when presented simultaneously with adult estrus females and also show increased sexual behavior towards juvenile and adult males. Lack of response to Esp1. Ref.3 Ref.4 Ref.5 Ref.6

Sequence similarities

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC2 sub-subfamily. [View classification]

Contains 3 ANK repeats.

Ontologies

Keywords
   Biological processCalcium transport
Ion transport
Transport
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainANK repeat
Repeat
Transmembrane
Transmembrane helix
   LigandCalcium
   Molecular functionCalcium channel
Ion channel
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processacrosome reaction

Inferred from mutant phenotype PubMed 11331878. Source: MGI

calcium ion transmembrane transport

Inferred from direct assay Ref.1. Source: GOC

calcium ion transport

Inferred from mutant phenotype PubMed 11331878. Source: MGI

female courtship behavior

Inferred from mutant phenotype PubMed 17676034. Source: MGI

inter-male aggressive behavior

Inferred from mutant phenotype Ref.4. Source: MGI

mating behavior, sex discrimination

Inferred from mutant phenotype Ref.4Ref.3. Source: MGI

negative regulation of male mating behavior

Inferred from genetic interaction Ref.6. Source: MGI

positive regulation of cytosolic calcium ion concentration

Inferred from physical interaction Ref.1. Source: MGI

response to pheromone

Inferred from mutant phenotype Ref.4Ref.3Ref.6. Source: MGI

single strand break repair

Inferred from electronic annotation. Source: InterPro

territorial aggressive behavior

Inferred from mutant phenotype Ref.3PubMed 17676034. Source: MGI

   Cellular_componentGolgi membrane

Inferred from direct assay Ref.2. Source: MGI

dendrite membrane

Inferred from mutant phenotype PubMed 14642279. Source: MGI

endomembrane system

Inferred from direct assay Ref.2. Source: MGI

endoplasmic reticulum membrane

Inferred from direct assay Ref.2. Source: MGI

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

nuclear envelope

Inferred from direct assay Ref.2. Source: MGI

nucleus

Inferred from electronic annotation. Source: InterPro

plasma membrane

Inferred from direct assay PubMed 14699131. Source: MGI

   Molecular_functioncalcium channel activity

Inferred from electronic annotation. Source: UniProtKB-KW

calmodulin binding

Inferred from direct assay PubMed 12601176. Source: MGI

damaged DNA binding

Inferred from electronic annotation. Source: InterPro

diacylglycerol binding

Inferred from mutant phenotype PubMed 14642279. Source: MGI

inositol 1,4,5 trisphosphate binding

Inferred from direct assay PubMed 14505576. Source: BHF-UCL

store-operated calcium channel activity

Inferred from direct assay Ref.1. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9R244-1)

Also known as: A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9R244-2)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     1-111: MLMSRTDSKS...RRTFFPDPQT → MGTKTHPVVPW
Isoform 3 (identifier: Q9R244-3)

Also known as: Alpha;

The sequence of this isoform differs from the canonical sequence as follows:
     2-287: Missing.
Isoform 4 (identifier: Q9R244-4)

Also known as: Beta;

The sequence of this isoform differs from the canonical sequence as follows:
     2-283: Missing.
     284-287: LVPK → DPLS

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11721172Short transient receptor potential channel 2
PRO_0000215307

Regions

Topological domain1 – 659659Cytoplasmic Potential
Transmembrane660 – 68021Helical; Potential
Topological domain681 – 70222Extracellular Potential
Transmembrane703 – 72321Helical; Potential
Topological domain724 – 73815Cytoplasmic Potential
Transmembrane739 – 75921Helical; Potential
Topological domain760 – 78930Extracellular Potential
Transmembrane790 – 81021Helical; Potential
Topological domain811 – 83323Cytoplasmic Potential
Transmembrane834 – 85421Helical; Potential
Topological domain855 – 89945Extracellular Potential
Transmembrane900 – 92021Helical; Potential
Topological domain921 – 1172252Cytoplasmic Potential
Repeat301 – 33030ANK 1
Repeat377 – 40630ANK 2
Repeat430 – 45930ANK 3
Compositional bias1140 – 11434Poly-Ser

Natural variations

Alternative sequence1 – 111111MLMSR…PDPQT → MGTKTHPVVPW in isoform 2.
VSP_006562
Alternative sequence2 – 287286Missing in isoform 3.
VSP_006564
Alternative sequence2 – 283282Missing in isoform 4.
VSP_006563
Alternative sequence284 – 2874LVPK → DPLS in isoform 4.
VSP_006565

Experimental info

Sequence conflict6281K → R in AAD17195. Ref.1
Sequence conflict6281K → R in AAD17196. Ref.1
Sequence conflict6331F → S in AAD17195. Ref.1
Sequence conflict6331F → S in AAD17196. Ref.1
Sequence conflict6531Q → R in AAG29951. Ref.2
Sequence conflict6531Q → R in AAG29950. Ref.2
Sequence conflict7741Y → C in AAD17195. Ref.1
Sequence conflict7741Y → C in AAD17196. Ref.1
Sequence conflict7971A → T in AAD17195. Ref.1
Sequence conflict7971A → T in AAD17196. Ref.1
Sequence conflict11611L → P in AAG29951. Ref.2
Sequence conflict11611L → P in AAG29950. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (A) [UniParc].

Last modified March 23, 2010. Version 2.
Checksum: D9A9A9FEE01D1536

FASTA1,172130,531
        10         20         30         40         50         60 
MLMSRTDSKS GKNRSGVRMF KDGDFLTPAS GESWDRLRLT CSQPFTRHQS FGLAFLRVRS 

        70         80         90        100        110        120 
SLGSLADPVV DPSAPGSSGL NQNSTDVLES DPRPWLTNPS IRRTFFPDPQ TSTKEISELK 

       130        140        150        160        170        180 
GMLKQLQPGP LGRAARMVLS AARKAPPASV VSPNNSHGEP GPSRAESAEP RAEEPNRKTA 

       190        200        210        220        230        240 
VGRRKRRKVQ EPRRSLSNSS SQPNRRTGRT RQRQHRPQTK SDDGGVQAAG QCPICAGFFS 

       250        260        270        280        290        300 
IETLPQHAAT CGESPPPQPA SPASLSSSES VLRRHHVALT PVPLVPKPQP NWTEIVNKKL 

       310        320        330        340        350        360 
KFPPTLLRAI QEGQLGLVQQ LLESSSDASG AGPGGPLRNV EESEDRSWRE ALNLAIRLGH 

       370        380        390        400        410        420 
EVITDVLLAN VKFDFRQIHE ALLVAVDTNQ PAVVRRLLAR LEREKGRKVD TKSFSLAFFD 

       430        440        450        460        470        480 
SSIDGSRFAP GVTPLTLACQ KDLYEIAQLL MDQGHTIARP HPVSCACLEC SNARRYDLLK 

       490        500        510        520        530        540 
FSLSRINTYR GIASRAHLSL ASEDAMLAAF QLSRELRRLA RKEPEFKPQY IALESLCQDY 

       550        560        570        580        590        600 
GFELLGMCRN QSEVTAVLND LGEDSETEPE AEGLGQAFEE GIPNLARLRL AVNYNQKQFV 

       610        620        630        640        650        660 
AHPICQQVLS SIWCGNLAGW RGSTTIWKLF VAFLIFLTMP FLCIGYWLAP KSQLGRLLKI 

       670        680        690        700        710        720 
PVLKFLLHSA SYLWFLIFLL GESLVMETQL STFKGRSQSV WETSLHMIWV TGFLWFECKE 

       730        740        750        760        770        780 
VWIEGLRSYL LDWWNFLDVV ILSLYLASFA LRLLLAGLAY MHCRDASDST TCRYFTTAER 

       790        800        810        820        830        840 
SEWRTEDPQF LAEVLFAVTS MLSFTRLAYI LPAHESLGTL QISIGKMIDD MIRFMFILMI 

       850        860        870        880        890        900 
ILTAFLCGLN NIYVPYQESE KLGNFNETFQ FLFWTMFGME EHTVVDMPQF LVPEFVGRAM 

       910        920        930        940        950        960 
YGIFTIVMVI VLLNMLIAMI TNSFQKIEDD ADVEWKFARS KLYLSYFREG LTLPVPFNIL 

       970        980        990       1000       1010       1020 
PSPKAAFYLV RRIFRFLCCG SSCCKAKKSD YPPIGTFTNP GARAGSAGEG ERVSYRLRVI 

      1030       1040       1050       1060       1070       1080 
KALVQRYIET ARREFEETRR KDLGNRLTEL TKTVSRLQSE VASVQKNLAA GGAPRPPDGA 

      1090       1100       1110       1120       1130       1140 
SILSRYITRV RNSFQNLGPP TSDTPAELTM PGIVETEVSL GDGLDGTGEA GAPAPGEPGS 

      1150       1160       1170 
SSSAHVLVHR EQEAEGSGDL LLEGDLETKG ES 

« Hide

Isoform 2 (B) [UniParc].

Checksum: 0566C84794F190C6
Show »

FASTA1,072119,508
Isoform 3 (Alpha) [UniParc].

Checksum: 19A4673B0B861E56
Show »

FASTA88699,545
Isoform 4 (Beta) [UniParc].

Checksum: 50791C101A98C5B8
Show »

FASTA89099,957

References

[1]"Mouse trp2, the homologue of the human trpc2 pseudogene, encodes mTrp2, a store depletion-activated capacitative Ca2+ entry channel."
Vannier B., Peyton M., Boulay G., Brown D., Qin N., Jiang M., Zhu X., Birnbaumer L.
Proc. Natl. Acad. Sci. U.S.A. 96:2060-2064(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Testis.
[2]"Cloning, expression and subcellular localization of two novel splice variants of mouse transient receptor potential channel 2."
Hofmann T., Schaefer M., Schultz G., Gudermann T.
Biochem. J. 351:115-122(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4).
[3]"Altered sexual and social behaviors in trp2 mutant mice."
Leypold B.G., Yu C.R., Leinders-Zufall T., Kim M.M., Zufall F., Axel R.
Proc. Natl. Acad. Sci. U.S.A. 99:6376-6381(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[4]"Loss of sex discrimination and male-male aggression in mice deficient for TRP2."
Stowers L., Holy T.E., Meister M., Dulac C., Koentges G.
Science 295:1493-1500(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[5]"The male mouse pheromone ESP1 enhances female sexual receptive behaviour through a specific vomeronasal receptor."
Haga S., Hattori T., Sato T., Sato K., Matsuda S., Kobayakawa R., Sakano H., Yoshihara Y., Kikusui T., Touhara K.
Nature 466:118-122(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[6]"A juvenile mouse pheromone inhibits sexual behaviour through the vomeronasal system."
Ferrero D.M., Moeller L.M., Osakada T., Horio N., Li Q., Roy D.S., Cichy A., Spehr M., Touhara K., Liberles S.D.
Nature 502:368-371(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF111108 mRNA. Translation: AAD17196.1.
AF111107 mRNA. Translation: AAD17195.1.
AF230803 mRNA. Translation: AAG29951.1.
AF230802 mRNA. Translation: AAG29950.1.
RefSeqNP_001103367.1. NM_001109897.2.
UniGeneMm.292904.

3D structure databases

ProteinModelPortalQ9R244.
SMRQ9R244. Positions 2-60, 303-460.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000081903.

Chemistry

GuidetoPHARMACOLOGY487.

PTM databases

PhosphoSiteQ9R244.

Proteomic databases

PaxDbQ9R244.
PRIDEQ9R244.

Protocols and materials databases

DNASU22064.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000106943; ENSMUSP00000102556; ENSMUSG00000070425. [Q9R244-4]
GeneID22064.
KEGGmmu:22064.
UCSCuc009iqm.1. mouse. [Q9R244-3]
uc009iqn.1. mouse. [Q9R244-2]
uc012fqr.1. mouse. [Q9R244-1]
uc012fqs.1. mouse. [Q9R244-4]

Organism-specific databases

CTD7221.
MGIMGI:109527. Trpc2.

Phylogenomic databases

eggNOGNOG257288.
GeneTreeENSGT00680000099764.
HOGENOMHOG000137678.
HOVERGENHBG101351.
InParanoidQ9R244.
KOK04965.
OrthoDBEOG76X5ZD.
PhylomeDBQ9R244.

Gene expression databases

BgeeQ9R244.
GenevestigatorQ9R244.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
2.60.120.260. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR008979. Galactose-bd-like.
IPR005821. Ion_trans_dom.
IPR013555. TRP_dom.
IPR005458. TRPC2_channel.
IPR002153. TRPC_channel.
IPR002706. Xrcc1_N.
[Graphical view]
PANTHERPTHR10117. PTHR10117. 1 hit.
PTHR10117:SF6. PTHR10117:SF6. 1 hit.
PfamPF00520. Ion_trans. 1 hit.
PF08344. TRP_2. 1 hit.
PF01834. XRCC1_N. 1 hit.
[Graphical view]
PRINTSPR01097. TRNSRECEPTRP.
PR01643. TRPCHANNEL2.
SMARTSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
SSF49785. SSF49785. 1 hit.
ProtoNetSearch...

Other

NextBio301876.
PROQ9R244.
SOURCESearch...

Entry information

Entry nameTRPC2_MOUSE
AccessionPrimary (citable) accession number: Q9R244
Secondary accession number(s): Q9ES59, Q9ES60, Q9R243
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: March 23, 2010
Last modified: April 16, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot