Short transient receptor potential channel 2

Preferred order of sections

Names and origin

Protein names

Recommended name:
Short transient receptor potential channel 2
Short name=TrpC2

Alternative name(s):
Transient receptor protein 2
Short name=TRP-2
Short name=mTrp2

Gene names

Name:	Trpc2
Synonyms:	Trp2, Trrp2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1172 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. May also be activated by intracellular calcium store depletion.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Tissue specificity	Isoform 3 is ubiquitously expressed at low levels. Isoform 4 is expressed exclusively in vomeronasal organ.
Sequence similarities	Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC2 sub-subfamily. [View classification] Contains 3 ANK repeats.

Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	ANK repeat Repeat Transmembrane Transmembrane helix
Ligand	Calcium
Molecular function	Calcium channel Ion channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	acrosome reaction Inferred from mutant phenotype PubMed 11331878. Source: MGI elevation of cytosolic calcium ion concentration Inferred from physical interaction Ref.1. Source: MGI female courtship behavior Inferred from mutant phenotype PubMed 17676034. Source: MGI inter-male aggressive behavior Inferred from mutant phenotype PubMed 11823606. Source: MGI mating behavior, sex discrimination Inferred from mutant phenotype PubMed 11823606 PubMed 11972034. Source: MGI response to pheromone Inferred from mutant phenotype PubMed 11823606 PubMed 11972034. Source: MGI single strand break repair Inferred from electronic annotation. Source: InterPro territorial aggressive behavior Inferred from mutant phenotype PubMed 11972034 PubMed 17676034. Source: MGI
Cellular_component	Golgi membrane Inferred from direct assay Ref.2. Source: MGI dendrite membrane Inferred from mutant phenotype PubMed 14642279. Source: MGI endoplasmic reticulum membrane Inferred from direct assay Ref.2. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope Inferred from direct assay Ref.2. Source: MGI nucleus Inferred from electronic annotation. Source: InterPro
Molecular_function	calcium channel activity Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from direct assay PubMed 12601176. Source: MGI damaged DNA binding Inferred from electronic annotation. Source: InterPro diacylglycerol binding Inferred from mutant phenotype PubMed 14642279. Source: MGI inositol 1,4,5 trisphosphate binding Inferred from direct assay PubMed 14505576. Source: BHF-UCL store-operated calcium channel activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9R244-1) Also known as: A; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9R244-2) Also known as: B; The sequence of this isoform differs from the canonical sequence as follows: 1-111: MLMSRTDSKS...RRTFFPDPQT → MGTKTHPVVPW

Isoform 3 (identifier: Q9R244-3) Also known as: Alpha; The sequence of this isoform differs from the canonical sequence as follows: 2-287: Missing.

Isoform 4 (identifier: Q9R244-4) Also known as: Beta; The sequence of this isoform differs from the canonical sequence as follows: 2-283: Missing. 284-287: LVPK → DPLS

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1172

1172

Short transient receptor potential channel 2

PRO_0000215307

Regions

Topological domain

1 – 659

659

Cytoplasmic Potential

Transmembrane

660 – 680

21

Helical; Potential

Topological domain

681 – 702

22

Extracellular Potential

Transmembrane

703 – 723

21

Helical; Potential

Topological domain

724 – 738

15

Cytoplasmic Potential

Transmembrane

739 – 759

21

Helical; Potential

Topological domain

760 – 789

30

Extracellular Potential

Transmembrane

790 – 810

21

Helical; Potential

Topological domain

811 – 833

23

Cytoplasmic Potential

Transmembrane

834 – 854

21

Helical; Potential

Topological domain

855 – 899

45

Extracellular Potential

Transmembrane

900 – 920

21

Helical; Potential

Topological domain

921 – 1172

252

Cytoplasmic Potential

Repeat

301 – 330

30

ANK 1

Repeat

377 – 406

30

ANK 2

Repeat

430 – 459

30

ANK 3

Compositional bias

1140 – 1143

4

Poly-Ser

Natural variations

Alternative sequence

1 – 111

111

MLMSR…PDPQT → MGTKTHPVVPW in isoform 2.

VSP_006562

Alternative sequence

2 – 287

286

Missing in isoform 3.

VSP_006564

Alternative sequence

2 – 283

282

Missing in isoform 4.

VSP_006563

Alternative sequence

284 – 287

4

LVPK → DPLS in isoform 4.

VSP_006565

Experimental info

Sequence conflict

628

1

K → R in AAD17195. Ref.1

Sequence conflict

628

1

K → R in AAD17196. Ref.1

Sequence conflict

633

1

F → S in AAD17195. Ref.1

Sequence conflict

633

1

F → S in AAD17196. Ref.1

Sequence conflict

653

1

Q → R in AAG29951. Ref.2

Sequence conflict

653

1

Q → R in AAG29950. Ref.2

Sequence conflict

774

1

Y → C in AAD17195. Ref.1

Sequence conflict

774

1

Y → C in AAD17196. Ref.1

Sequence conflict

797

1

A → T in AAD17195. Ref.1

Sequence conflict

797

1

A → T in AAD17196. Ref.1

Sequence conflict

1161

1

L → P in AAG29951. Ref.2

Sequence conflict

1161

1

L → P in AAG29950. Ref.2

Sequences

Sequence		Length	Mass (Da)
Isoform 1 (A) [UniParc]. Last modified March 23, 2010. Version 2. Checksum: D9A9A9FEE01D1536 Show »	FASTA	1,172	130,531
10 20 30 40 50 60 MLMSRTDSKS GKNRSGVRMF KDGDFLTPAS GESWDRLRLT CSQPFTRHQS FGLAFLRVRS 70 80 90 100 110 120 SLGSLADPVV DPSAPGSSGL NQNSTDVLES DPRPWLTNPS IRRTFFPDPQ TSTKEISELK 130 140 150 160 170 180 GMLKQLQPGP LGRAARMVLS AARKAPPASV VSPNNSHGEP GPSRAESAEP RAEEPNRKTA 190 200 210 220 230 240 VGRRKRRKVQ EPRRSLSNSS SQPNRRTGRT RQRQHRPQTK SDDGGVQAAG QCPICAGFFS 250 260 270 280 290 300 IETLPQHAAT CGESPPPQPA SPASLSSSES VLRRHHVALT PVPLVPKPQP NWTEIVNKKL 310 320 330 340 350 360 KFPPTLLRAI QEGQLGLVQQ LLESSSDASG AGPGGPLRNV EESEDRSWRE ALNLAIRLGH 370 380 390 400 410 420 EVITDVLLAN VKFDFRQIHE ALLVAVDTNQ PAVVRRLLAR LEREKGRKVD TKSFSLAFFD 430 440 450 460 470 480 SSIDGSRFAP GVTPLTLACQ KDLYEIAQLL MDQGHTIARP HPVSCACLEC SNARRYDLLK 490 500 510 520 530 540 FSLSRINTYR GIASRAHLSL ASEDAMLAAF QLSRELRRLA RKEPEFKPQY IALESLCQDY 550 560 570 580 590 600 GFELLGMCRN QSEVTAVLND LGEDSETEPE AEGLGQAFEE GIPNLARLRL AVNYNQKQFV 610 620 630 640 650 660 AHPICQQVLS SIWCGNLAGW RGSTTIWKLF VAFLIFLTMP FLCIGYWLAP KSQLGRLLKI 670 680 690 700 710 720 PVLKFLLHSA SYLWFLIFLL GESLVMETQL STFKGRSQSV WETSLHMIWV TGFLWFECKE 730 740 750 760 770 780 VWIEGLRSYL LDWWNFLDVV ILSLYLASFA LRLLLAGLAY MHCRDASDST TCRYFTTAER 790 800 810 820 830 840 SEWRTEDPQF LAEVLFAVTS MLSFTRLAYI LPAHESLGTL QISIGKMIDD MIRFMFILMI 850 860 870 880 890 900 ILTAFLCGLN NIYVPYQESE KLGNFNETFQ FLFWTMFGME EHTVVDMPQF LVPEFVGRAM 910 920 930 940 950 960 YGIFTIVMVI VLLNMLIAMI TNSFQKIEDD ADVEWKFARS KLYLSYFREG LTLPVPFNIL 970 980 990 1000 1010 1020 PSPKAAFYLV RRIFRFLCCG SSCCKAKKSD YPPIGTFTNP GARAGSAGEG ERVSYRLRVI 1030 1040 1050 1060 1070 1080 KALVQRYIET ARREFEETRR KDLGNRLTEL TKTVSRLQSE VASVQKNLAA GGAPRPPDGA 1090 1100 1110 1120 1130 1140 SILSRYITRV RNSFQNLGPP TSDTPAELTM PGIVETEVSL GDGLDGTGEA GAPAPGEPGS 1150 1160 1170 SSSAHVLVHR EQEAEGSGDL LLEGDLETKG ES « Hide
Isoform 2 (B) [UniParc]. Checksum: 0566C84794F190C6 Show »	FASTA	1,072	119,508
10 20 30 40 50 60 MGTKTHPVVP WSTKEISELK GMLKQLQPGP LGRAARMVLS AARKAPPASV VSPNNSHGEP 70 80 90 100 110 120 GPSRAESAEP RAEEPNRKTA VGRRKRRKVQ EPRRSLSNSS SQPNRRTGRT RQRQHRPQTK 130 140 150 160 170 180 SDDGGVQAAG QCPICAGFFS IETLPQHAAT CGESPPPQPA SPASLSSSES VLRRHHVALT 190 200 210 220 230 240 PVPLVPKPQP NWTEIVNKKL KFPPTLLRAI QEGQLGLVQQ LLESSSDASG AGPGGPLRNV 250 260 270 280 290 300 EESEDRSWRE ALNLAIRLGH EVITDVLLAN VKFDFRQIHE ALLVAVDTNQ PAVVRRLLAR 310 320 330 340 350 360 LEREKGRKVD TKSFSLAFFD SSIDGSRFAP GVTPLTLACQ KDLYEIAQLL MDQGHTIARP 370 380 390 400 410 420 HPVSCACLEC SNARRYDLLK FSLSRINTYR GIASRAHLSL ASEDAMLAAF QLSRELRRLA 430 440 450 460 470 480 RKEPEFKPQY IALESLCQDY GFELLGMCRN QSEVTAVLND LGEDSETEPE AEGLGQAFEE 490 500 510 520 530 540 GIPNLARLRL AVNYNQKQFV AHPICQQVLS SIWCGNLAGW RGSTTIWKLF VAFLIFLTMP 550 560 570 580 590 600 FLCIGYWLAP KSQLGRLLKI PVLKFLLHSA SYLWFLIFLL GESLVMETQL STFKGRSQSV 610 620 630 640 650 660 WETSLHMIWV TGFLWFECKE VWIEGLRSYL LDWWNFLDVV ILSLYLASFA LRLLLAGLAY 670 680 690 700 710 720 MHCRDASDST TCRYFTTAER SEWRTEDPQF LAEVLFAVTS MLSFTRLAYI LPAHESLGTL 730 740 750 760 770 780 QISIGKMIDD MIRFMFILMI ILTAFLCGLN NIYVPYQESE KLGNFNETFQ FLFWTMFGME 790 800 810 820 830 840 EHTVVDMPQF LVPEFVGRAM YGIFTIVMVI VLLNMLIAMI TNSFQKIEDD ADVEWKFARS 850 860 870 880 890 900 KLYLSYFREG LTLPVPFNIL PSPKAAFYLV RRIFRFLCCG SSCCKAKKSD YPPIGTFTNP 910 920 930 940 950 960 GARAGSAGEG ERVSYRLRVI KALVQRYIET ARREFEETRR KDLGNRLTEL TKTVSRLQSE 970 980 990 1000 1010 1020 VASVQKNLAA GGAPRPPDGA SILSRYITRV RNSFQNLGPP TSDTPAELTM PGIVETEVSL 1030 1040 1050 1060 1070 GDGLDGTGEA GAPAPGEPGS SSSAHVLVHR EQEAEGSGDL LLEGDLETKG ES « Hide
Isoform 3 (Alpha) [UniParc]. Checksum: 19A4673B0B861E56 Show »	FASTA	886	99,545
10 20 30 40 50 60 MPQPNWTEIV NKKLKFPPTL LRAIQEGQLG LVQQLLESSS DASGAGPGGP LRNVEESEDR 70 80 90 100 110 120 SWREALNLAI RLGHEVITDV LLANVKFDFR QIHEALLVAV DTNQPAVVRR LLARLEREKG 130 140 150 160 170 180 RKVDTKSFSL AFFDSSIDGS RFAPGVTPLT LACQKDLYEI AQLLMDQGHT IARPHPVSCA 190 200 210 220 230 240 CLECSNARRY DLLKFSLSRI NTYRGIASRA HLSLASEDAM LAAFQLSREL RRLARKEPEF 250 260 270 280 290 300 KPQYIALESL CQDYGFELLG MCRNQSEVTA VLNDLGEDSE TEPEAEGLGQ AFEEGIPNLA 310 320 330 340 350 360 RLRLAVNYNQ KQFVAHPICQ QVLSSIWCGN LAGWRGSTTI WKLFVAFLIF LTMPFLCIGY 370 380 390 400 410 420 WLAPKSQLGR LLKIPVLKFL LHSASYLWFL IFLLGESLVM ETQLSTFKGR SQSVWETSLH 430 440 450 460 470 480 MIWVTGFLWF ECKEVWIEGL RSYLLDWWNF LDVVILSLYL ASFALRLLLA GLAYMHCRDA 490 500 510 520 530 540 SDSTTCRYFT TAERSEWRTE DPQFLAEVLF AVTSMLSFTR LAYILPAHES LGTLQISIGK 550 560 570 580 590 600 MIDDMIRFMF ILMIILTAFL CGLNNIYVPY QESEKLGNFN ETFQFLFWTM FGMEEHTVVD 610 620 630 640 650 660 MPQFLVPEFV GRAMYGIFTI VMVIVLLNML IAMITNSFQK IEDDADVEWK FARSKLYLSY 670 680 690 700 710 720 FREGLTLPVP FNILPSPKAA FYLVRRIFRF LCCGSSCCKA KKSDYPPIGT FTNPGARAGS 730 740 750 760 770 780 AGEGERVSYR LRVIKALVQR YIETARREFE ETRRKDLGNR LTELTKTVSR LQSEVASVQK 790 800 810 820 830 840 NLAAGGAPRP PDGASILSRY ITRVRNSFQN LGPPTSDTPA ELTMPGIVET EVSLGDGLDG 850 860 870 880 TGEAGAPAPG EPGSSSSAHV LVHREQEAEG SGDLLLEGDL ETKGES « Hide
Isoform 4 (Beta) [UniParc]. Checksum: 50791C101A98C5B8 Show »	FASTA	890	99,957
10 20 30 40 50 60 MDPLSPQPNW TEIVNKKLKF PPTLLRAIQE GQLGLVQQLL ESSSDASGAG PGGPLRNVEE 70 80 90 100 110 120 SEDRSWREAL NLAIRLGHEV ITDVLLANVK FDFRQIHEAL LVAVDTNQPA VVRRLLARLE 130 140 150 160 170 180 REKGRKVDTK SFSLAFFDSS IDGSRFAPGV TPLTLACQKD LYEIAQLLMD QGHTIARPHP 190 200 210 220 230 240 VSCACLECSN ARRYDLLKFS LSRINTYRGI ASRAHLSLAS EDAMLAAFQL SRELRRLARK 250 260 270 280 290 300 EPEFKPQYIA LESLCQDYGF ELLGMCRNQS EVTAVLNDLG EDSETEPEAE GLGQAFEEGI 310 320 330 340 350 360 PNLARLRLAV NYNQKQFVAH PICQQVLSSI WCGNLAGWRG STTIWKLFVA FLIFLTMPFL 370 380 390 400 410 420 CIGYWLAPKS QLGRLLKIPV LKFLLHSASY LWFLIFLLGE SLVMETQLST FKGRSQSVWE 430 440 450 460 470 480 TSLHMIWVTG FLWFECKEVW IEGLRSYLLD WWNFLDVVIL SLYLASFALR LLLAGLAYMH 490 500 510 520 530 540 CRDASDSTTC RYFTTAERSE WRTEDPQFLA EVLFAVTSML SFTRLAYILP AHESLGTLQI 550 560 570 580 590 600 SIGKMIDDMI RFMFILMIIL TAFLCGLNNI YVPYQESEKL GNFNETFQFL FWTMFGMEEH 610 620 630 640 650 660 TVVDMPQFLV PEFVGRAMYG IFTIVMVIVL LNMLIAMITN SFQKIEDDAD VEWKFARSKL 670 680 690 700 710 720 YLSYFREGLT LPVPFNILPS PKAAFYLVRR IFRFLCCGSS CCKAKKSDYP PIGTFTNPGA 730 740 750 760 770 780 RAGSAGEGER VSYRLRVIKA LVQRYIETAR REFEETRRKD LGNRLTELTK TVSRLQSEVA 790 800 810 820 830 840 SVQKNLAAGG APRPPDGASI LSRYITRVRN SFQNLGPPTS DTPAELTMPG IVETEVSLGD 850 860 870 880 890 GLDGTGEAGA PAPGEPGSSS SAHVLVHREQ EAEGSGDLLL EGDLETKGES « Hide

References

[1]	"Mouse trp2, the homologue of the human trpc2 pseudogene, encodes mTrp2, a store depletion-activated capacitative Ca2+ entry channel." Vannier B., Peyton M., Boulay G., Brown D., Qin N., Jiang M., Zhu X., Birnbaumer L. Proc. Natl. Acad. Sci. U.S.A. 96:2060-2064(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Testis.
[2]	"Cloning, expression and subcellular localization of two novel splice variants of mouse transient receptor potential channel 2." Hofmann T., Schaefer M., Schultz G., Gudermann T. Biochem. J. 351:115-122(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF111108 mRNA. Translation: AAD17196.1. AF111107 mRNA. Translation: AAD17195.1. AF230803 mRNA. Translation: AAG29951.1. AF230802 mRNA. Translation: AAG29950.1.
IPI	IPI00230561. IPI00230562. IPI00230563. IPI00974849.
RefSeq	NP_001103367.1. NM_001109897.1. NP_035774.2. NM_011644.2.
UniGene	Mm.292904.
3D structure databases
ProteinModelPortal	Q9R244.
SMR	Q9R244. Positions 2-60, 304-458.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000081903.
PTM databases
PhosphoSite	Q9R244.
Proteomic databases
PaxDb	Q9R244.
PRIDE	Q9R244.
Protocols and materials databases
DNASU	22064.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000106943; ENSMUSP00000102556; ENSMUSG00000070425.
GeneID	22064.
KEGG	mmu:22064.
UCSC	uc009iql.1. mouse. uc009iqm.1. mouse. uc009iqn.1. mouse. uc012fqs.1. mouse.
Organism-specific databases
CTD	7221.
MGI	MGI:109527. Trpc2.
Phylogenomic databases
eggNOG	NOG257288.
GeneTree	ENSGT00680000099764.
HOGENOM	HOG000137678.
HOVERGEN	HBG101351.
InParanoid	Q9R244.
KO	K04965.
OrthoDB	EOG49CQ72.
Gene expression databases
ArrayExpress	Q9R244.
Bgee	Q9R244.
Genevestigator	Q9R244.
GermOnline	ENSMUSG00000070425. Mus musculus.
Family and domain databases
Gene3D	1.25.40.20. 1 hit.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR008979. Galactose-bd-like. IPR005821. Ion_trans_dom. IPR013555. TRP_dom. IPR005458. TRPC2_channel. IPR002153. TRPC_channel. IPR002706. Xrcc1_N. [Graphical view]
PANTHER	PTHR10117. PTHR10117. 1 hit. PTHR10117:SF6. PTHR10117:SF6. 1 hit.
Pfam	PF00520. Ion_trans. 1 hit. PF08344. TRP_2. 1 hit. PF01834. XRCC1_N. 1 hit. [Graphical view]
PRINTS	PR01097. TRNSRECEPTRP. PR01643. TRPCHANNEL2.
SMART	SM00248. ANK. 2 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit. SSF49785. Gal_bind_like. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. False negative. PS50088. ANK_REPEAT. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	301876.
SOURCE	Search...

Entry information

Entry name

TRPC2_MOUSE

Accession

Primary (citable) accession number: Q9R244
Secondary accession number(s): Q9ES59, Q9ES60, Q9R243

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	March 23, 2010
Last modified:	May 1, 2013
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents