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Protein

Tapasin

Gene

Tapbp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei431 – 4311May be involved in interaction with TAP

GO - Molecular functioni

GO - Biological processi

  • antigen processing and presentation of endogenous peptide antigen via MHC class I Source: InterPro
  • antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Source: MGI
  • defense response Source: MGI
  • MHC class I protein complex assembly Source: Ensembl
  • peptide antigen stabilization Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_308964. ER-Phagosome pathway.
REACT_339401. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.
REACT_341075. ER-Phagosome pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Tapasin
Short name:
TPN
Short name:
TPSN
Alternative name(s):
TAP-associated protein
TAP-binding protein
Gene namesi
Name:Tapbp
Synonyms:Tapa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1201689. Tapbp.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 416393LumenalSequence AnalysisAdd
BLAST
Transmembranei417 – 43721HelicalSequence AnalysisAdd
BLAST
Topological domaini438 – 46528CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323By similarityAdd
BLAST
Chaini24 – 465442TapasinPRO_0000014991Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 94PROSITE-ProRule annotation
Disulfide bondi118 – 118Interchain (with C-57 in PDIA3)PROSITE-ProRule annotation
Glycosylationi256 – 2561N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi318 ↔ 385PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9R233.
PaxDbiQ9R233.
PRIDEiQ9R233.

PTM databases

PhosphoSiteiQ9R233.

Expressioni

Gene expression databases

BgeeiQ9R233.
CleanExiMM_TAPBP.
ExpressionAtlasiQ9R233. baseline and differential.
GenevestigatoriQ9R233.

Interactioni

Subunit structurei

Interacts with TAP1 and is thus a subunit of the TAP complex. Interaction with TAP1 is TAP2 independent and is required for efficient peptide-TAP interaction. Obligatory mediator for the interaction between newly assembled MHC class I molecules, calreticulin, ERp57 and TAP. Up to 4 MHC class I/tapasin complexes bind to 1 TAP.

Protein-protein interaction databases

IntActiQ9R233. 2 interactions.
MINTiMINT-4138357.

Structurei

3D structure databases

ProteinModelPortaliQ9R233.
SMRiQ9R233. Positions 24-404.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini295 – 402108Ig-like C1-typeAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi37 – 404Poly-Gly

Domaini

The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminal region (By similarity).By similarity

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG41000.
HOGENOMiHOG000231214.
HOVERGENiHBG005156.
InParanoidiQ9R233.
KOiK08058.
PhylomeDBiQ9R233.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR003599. Ig_sub.
IPR008056. Tapasin.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
[Graphical view]
PRINTSiPR01669. TAPASIN.
SMARTiSM00409. IG. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q9R233-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKPLLLLVAV ALGLATVVSV VSAGPEAIEC WFVEDAGGGG LSKKPATLLL
60 70 80 90 100
RHGPRGPPPR PDLDPKLYFK VDDPAGMLLA AFRRYPAGAS APHCEMSRFI
110 120 130 140 150
PFPASAKWAR SLSPEQNCPR ALDGDWLLVS VSSTLFSLSS LLRPQPEPLR
160 170 180 190 200
EPVVITMATV VLTVLTHNPA PRVQLGKDAV LDLRFAYAPS ALEGSPSLDA
210 220 230 240 250
GPPPFGLEWR RQHRGKGHLL LAATPGLAGR MPPAQEKATA FAAWDDDEPW
260 270 280 290 300
GPWTGNGTFW LPAVKPSQEG VYLATVHLPY LQGQVSLELT VHKAPRVSLT
310 320 330 340 350
PAPVVWAAPG EAPPELLCLA SHFFPAEGLE VKWELRGGPG GSSRKVEGKT
360 370 380 390 400
WLSTIRHHSD GSVSQSGHLQ LPPVTAKQHG VHYVCRVYHS SLPASGRSAD
410 420 430 440 450
VTLEVAGFSG PSIEDGIGLF LSAFLLLGLL KVLGWLAAYW TIPEVSKEKA
460
TAASLTIPRN SKKSQ
Length:465
Mass (Da):49,736
Last modified:October 3, 2003 - v2
Checksum:iDCADF445A1437C3C
GO
Isoform Short (identifier: Q9R233-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     407-436: Missing.

Show »
Length:435
Mass (Da):46,621
Checksum:iEF81A50547D8A004
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171V → F in AAC61676 (PubMed:9716645).Curated
Sequence conflicti274 – 2741A → G in AAC61676 (PubMed:9716645).Curated
Sequence conflicti294 – 2941A → G in AAC61676 (PubMed:9716645).Curated
Sequence conflicti326 – 3261A → S in AAD17964 (PubMed:10085102).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei407 – 43630Missing in isoform Short. CuratedVSP_002578Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106278 mRNA. Translation: AAD17964.1.
AF110520 Genomic DNA. Translation: AAC97973.1.
AF100956 Genomic DNA. Translation: AAC69893.1.
AF043943 mRNA. Translation: AAC61676.1.
AJ316612 Genomic DNA. Translation: CAC69993.1.
AJ316613 mRNA. Translation: CAC70721.2.
RefSeqiNP_033344.1. NM_009318.2. [Q9R233-1]
UniGeneiMm.154457.

Genome annotation databases

GeneIDi21356.
KEGGimmu:21356.
UCSCiuc008cae.1. mouse. [Q9R233-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106278 mRNA. Translation: AAD17964.1.
AF110520 Genomic DNA. Translation: AAC97973.1.
AF100956 Genomic DNA. Translation: AAC69893.1.
AF043943 mRNA. Translation: AAC61676.1.
AJ316612 Genomic DNA. Translation: CAC69993.1.
AJ316613 mRNA. Translation: CAC70721.2.
RefSeqiNP_033344.1. NM_009318.2. [Q9R233-1]
UniGeneiMm.154457.

3D structure databases

ProteinModelPortaliQ9R233.
SMRiQ9R233. Positions 24-404.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9R233. 2 interactions.
MINTiMINT-4138357.

PTM databases

PhosphoSiteiQ9R233.

Proteomic databases

MaxQBiQ9R233.
PaxDbiQ9R233.
PRIDEiQ9R233.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi21356.
KEGGimmu:21356.
UCSCiuc008cae.1. mouse. [Q9R233-1]

Organism-specific databases

CTDi6892.
MGIiMGI:1201689. Tapbp.

Phylogenomic databases

eggNOGiNOG41000.
HOGENOMiHOG000231214.
HOVERGENiHBG005156.
InParanoidiQ9R233.
KOiK08058.
PhylomeDBiQ9R233.

Enzyme and pathway databases

ReactomeiREACT_308964. ER-Phagosome pathway.
REACT_339401. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.
REACT_341075. ER-Phagosome pathway.

Miscellaneous databases

ChiTaRSiTapbp. mouse.
NextBioi300560.
PROiQ9R233.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R233.
CleanExiMM_TAPBP.
ExpressionAtlasiQ9R233. baseline and differential.
GenevestigatoriQ9R233.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR003599. Ig_sub.
IPR008056. Tapasin.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
[Graphical view]
PRINTSiPR01669. TAPASIN.
SMARTiSM00409. IG. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Peptide-bound major histocompatibility complex class I molecules associate with tapasin before dissociation from transporter associated with antigen processing."
    Li S., Paulsson K.M., Sjoegren H.-O., Wang P.
    J. Biol. Chem. 274:8649-8654(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
    Tissue: Liver.
  2. "Sequence of the mouse major histocomaptibility locus class II region."
    Rowen L., Qin S., Madan A., Loretz C., Hall J., James R., Dors M., Shaffer T., Abbasi N., Ratcliffe A., Dickhoff R., Lasky S., Hood L.
    Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS LONG AND SHORT).
    Strain: 129.
  3. "Sequence, linkage to H2-K, and function of mouse tapasin in MHC class I assembly."
    Grandea A.G. III, Comber P.G., Wenderfer S.E., Schoenhals G., Frueh K., Monaco J.J., Spies T.
    Immunogenetics 48:260-265(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
  4. "Regulation of the expression of mouse TAP-associated glycoprotein (tapasin) by cytokines."
    Abarca-Heidemann K., Friederichs S., Klamp T., Boehm U., Guethlein L.A., Ortmann B.
    Immunol. Lett. 83:197-207(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM LONG).
    Strain: C57BL/10.

Entry informationi

Entry nameiTPSN_MOUSE
AccessioniPrimary (citable) accession number: Q9R233
Secondary accession number(s): O70317
, Q91YE0, Q91YE1, Q9QWT7, Q9Z1W3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: October 3, 2003
Last modified: April 1, 2015
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.