Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9R233

- TPSN_MOUSE

UniProt

Q9R233 - TPSN_MOUSE

Protein

Tapasin

Gene

Tapbp

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (03 Oct 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei431 – 4311May be involved in interaction with TAP

    GO - Molecular functioni

    1. protein binding Source: MGI

    GO - Biological processi

    1. antigen processing and presentation of endogenous peptide antigen via MHC class I Source: InterPro
    2. antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Source: MGI
    3. defense response Source: MGI
    4. MHC class I protein complex assembly Source: Ensembl
    5. peptide antigen stabilization Source: UniProtKB

    Enzyme and pathway databases

    ReactomeiREACT_197102. ER-Phagosome pathway.
    REACT_199105. ER-Phagosome pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tapasin
    Short name:
    TPN
    Short name:
    TPSN
    Alternative name(s):
    TAP-associated protein
    TAP-binding protein
    Gene namesi
    Name:Tapbp
    Synonyms:Tapa
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1201689. Tapbp.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: MGI
    2. MHC class I peptide loading complex Source: Ensembl
    3. phagocytic vesicle membrane Source: Reactome

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323By similarityAdd
    BLAST
    Chaini24 – 465442TapasinPRO_0000014991Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi30 ↔ 94PROSITE-ProRule annotation
    Disulfide bondi118 – 118Interchain (with C-57 in PDIA3)PROSITE-ProRule annotation
    Glycosylationi256 – 2561N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi318 ↔ 385PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ9R233.
    PaxDbiQ9R233.
    PRIDEiQ9R233.

    PTM databases

    PhosphoSiteiQ9R233.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9R233.
    BgeeiQ9R233.
    CleanExiMM_TAPBP.
    GenevestigatoriQ9R233.

    Interactioni

    Subunit structurei

    Interacts with TAP1 and is thus a subunit of the TAP complex. Interaction with TAP1 is TAP2 independent and is required for efficient peptide-TAP interaction. Obligatory mediator for the interaction between newly assembled MHC class I molecules, calreticulin, ERp57 and TAP. Up to 4 MHC class I/tapasin complexes bind to 1 TAP.

    Protein-protein interaction databases

    IntActiQ9R233. 2 interactions.
    MINTiMINT-4138357.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R233.
    SMRiQ9R233. Positions 24-404.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 416393LumenalSequence AnalysisAdd
    BLAST
    Topological domaini438 – 46528CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei417 – 43721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini295 – 402108Ig-like C1-typeAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi37 – 404Poly-Gly

    Domaini

    The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminal region By similarity.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG41000.
    HOGENOMiHOG000231214.
    HOVERGENiHBG005156.
    InParanoidiQ9R233.
    KOiK08058.
    PhylomeDBiQ9R233.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    InterProiIPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003006. Ig/MHC_CS.
    IPR003597. Ig_C1-set.
    IPR003599. Ig_sub.
    IPR008056. Tapasin.
    [Graphical view]
    PfamiPF07654. C1-set. 1 hit.
    [Graphical view]
    PRINTSiPR01669. TAPASIN.
    SMARTiSM00409. IG. 1 hit.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 1 hit.
    PS00290. IG_MHC. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: Q9R233-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKPLLLLVAV ALGLATVVSV VSAGPEAIEC WFVEDAGGGG LSKKPATLLL    50
    RHGPRGPPPR PDLDPKLYFK VDDPAGMLLA AFRRYPAGAS APHCEMSRFI 100
    PFPASAKWAR SLSPEQNCPR ALDGDWLLVS VSSTLFSLSS LLRPQPEPLR 150
    EPVVITMATV VLTVLTHNPA PRVQLGKDAV LDLRFAYAPS ALEGSPSLDA 200
    GPPPFGLEWR RQHRGKGHLL LAATPGLAGR MPPAQEKATA FAAWDDDEPW 250
    GPWTGNGTFW LPAVKPSQEG VYLATVHLPY LQGQVSLELT VHKAPRVSLT 300
    PAPVVWAAPG EAPPELLCLA SHFFPAEGLE VKWELRGGPG GSSRKVEGKT 350
    WLSTIRHHSD GSVSQSGHLQ LPPVTAKQHG VHYVCRVYHS SLPASGRSAD 400
    VTLEVAGFSG PSIEDGIGLF LSAFLLLGLL KVLGWLAAYW TIPEVSKEKA 450
    TAASLTIPRN SKKSQ 465
    Length:465
    Mass (Da):49,736
    Last modified:October 3, 2003 - v2
    Checksum:iDCADF445A1437C3C
    GO
    Isoform Short (identifier: Q9R233-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         407-436: Missing.

    Show »
    Length:435
    Mass (Da):46,621
    Checksum:iEF81A50547D8A004
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 171V → F in AAC61676. (PubMed:9716645)Curated
    Sequence conflicti274 – 2741A → G in AAC61676. (PubMed:9716645)Curated
    Sequence conflicti294 – 2941A → G in AAC61676. (PubMed:9716645)Curated
    Sequence conflicti326 – 3261A → S in AAD17964. (PubMed:10085102)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei407 – 43630Missing in isoform Short. CuratedVSP_002578Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF106278 mRNA. Translation: AAD17964.1.
    AF110520 Genomic DNA. Translation: AAC97973.1.
    AF100956 Genomic DNA. Translation: AAC69893.1.
    AF043943 mRNA. Translation: AAC61676.1.
    AJ316612 Genomic DNA. Translation: CAC69993.1.
    AJ316613 mRNA. Translation: CAC70721.2.
    RefSeqiNP_033344.1. NM_009318.2. [Q9R233-1]
    UniGeneiMm.154457.

    Genome annotation databases

    GeneIDi21356.
    KEGGimmu:21356.
    UCSCiuc008cae.1. mouse. [Q9R233-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF106278 mRNA. Translation: AAD17964.1 .
    AF110520 Genomic DNA. Translation: AAC97973.1 .
    AF100956 Genomic DNA. Translation: AAC69893.1 .
    AF043943 mRNA. Translation: AAC61676.1 .
    AJ316612 Genomic DNA. Translation: CAC69993.1 .
    AJ316613 mRNA. Translation: CAC70721.2 .
    RefSeqi NP_033344.1. NM_009318.2. [Q9R233-1 ]
    UniGenei Mm.154457.

    3D structure databases

    ProteinModelPortali Q9R233.
    SMRi Q9R233. Positions 24-404.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9R233. 2 interactions.
    MINTi MINT-4138357.

    PTM databases

    PhosphoSitei Q9R233.

    Proteomic databases

    MaxQBi Q9R233.
    PaxDbi Q9R233.
    PRIDEi Q9R233.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 21356.
    KEGGi mmu:21356.
    UCSCi uc008cae.1. mouse. [Q9R233-1 ]

    Organism-specific databases

    CTDi 6892.
    MGIi MGI:1201689. Tapbp.

    Phylogenomic databases

    eggNOGi NOG41000.
    HOGENOMi HOG000231214.
    HOVERGENi HBG005156.
    InParanoidi Q9R233.
    KOi K08058.
    PhylomeDBi Q9R233.

    Enzyme and pathway databases

    Reactomei REACT_197102. ER-Phagosome pathway.
    REACT_199105. ER-Phagosome pathway.

    Miscellaneous databases

    ChiTaRSi TAPBP. mouse.
    NextBioi 300560.
    PROi Q9R233.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9R233.
    Bgeei Q9R233.
    CleanExi MM_TAPBP.
    Genevestigatori Q9R233.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    InterProi IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003006. Ig/MHC_CS.
    IPR003597. Ig_C1-set.
    IPR003599. Ig_sub.
    IPR008056. Tapasin.
    [Graphical view ]
    Pfami PF07654. C1-set. 1 hit.
    [Graphical view ]
    PRINTSi PR01669. TAPASIN.
    SMARTi SM00409. IG. 1 hit.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 1 hit.
    PS00290. IG_MHC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Peptide-bound major histocompatibility complex class I molecules associate with tapasin before dissociation from transporter associated with antigen processing."
      Li S., Paulsson K.M., Sjoegren H.-O., Wang P.
      J. Biol. Chem. 274:8649-8654(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
      Tissue: Liver.
    2. "Sequence of the mouse major histocomaptibility locus class II region."
      Rowen L., Qin S., Madan A., Loretz C., Hall J., James R., Dors M., Shaffer T., Abbasi N., Ratcliffe A., Dickhoff R., Lasky S., Hood L.
      Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS LONG AND SHORT).
      Strain: 129.
    3. "Sequence, linkage to H2-K, and function of mouse tapasin in MHC class I assembly."
      Grandea A.G. III, Comber P.G., Wenderfer S.E., Schoenhals G., Frueh K., Monaco J.J., Spies T.
      Immunogenetics 48:260-265(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
    4. "Regulation of the expression of mouse TAP-associated glycoprotein (tapasin) by cytokines."
      Abarca-Heidemann K., Friederichs S., Klamp T., Boehm U., Guethlein L.A., Ortmann B.
      Immunol. Lett. 83:197-207(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORM LONG).
      Strain: C57BL/10.

    Entry informationi

    Entry nameiTPSN_MOUSE
    AccessioniPrimary (citable) accession number: Q9R233
    Secondary accession number(s): O70317
    , Q91YE0, Q91YE1, Q9QWT7, Q9Z1W3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: October 3, 2003
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3