Q9R233 (TPSN_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tapasin
Short name=TPN
Short name=TPSN

Alternative name(s):
TAP-associated protein
TAP-binding protein

Gene names

Name:	Tapbp
Synonyms:	Tapa

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).
Subunit structure	Interacts with TAP1 and is thus a subunit of the TAP complex. Interaction with TAP1 is TAP2 independent and is required for efficient peptide-TAP interaction. Obligatory mediator for the interaction between newly assembled MHC class I molecules, calreticulin, ERp57 and TAP. Up to 4 MHC class I/tapasin complexes bind to 1 TAP.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein Probable.
Domain	The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminus region By similarity.
Sequence similarities	Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation of endogenous peptide antigen via MHC class I Inferred from electronic annotation. Source: InterPro antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Inferred from direct assay Ref.3. Source: MGI defense response Inferred from mutant phenotype PubMed 10981964. Source: MGI peptide antigen stabilization Inferred from mutant phenotype PubMed 12594855. Source: UniProtKB
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: Compara TAP complex Inferred from electronic annotation. Source: Compara integral to plasma membrane Traceable author statement PubMed 10981964. Source: MGI phagocytic vesicle membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q9R233-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q9R233-2)

The sequence of this isoform differs from the canonical sequence as follows:
     407-436: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	By similarity
	Chain	24 – 465	442	Tapasin		PRO_0000014991
Regions
	Topological domain	24 – 416	393	Lumenal Potential
	Transmembrane	417 – 437	21	Helical; Potential
	Topological domain	438 – 465	28	Cytoplasmic Potential
	Domain	295 – 402	108	Ig-like C1-type
	Compositional bias	37 – 40	4	Poly-Gly
Sites
	Site	431	1	May be involved in interaction with TAP
Amino acid modifications
	Glycosylation	256	1	N-linked (GlcNAc...) Potential
	Disulfide bond	30 ↔ 94		By similarity
	Disulfide bond	118		Interchain (with C-57 in PDIA3) By similarity
	Disulfide bond	318 ↔ 385		By similarity
Natural variations
	Alternative sequence	407 – 436	30	Missing in isoform Short.		VSP_002578
Experimental info
	Sequence conflict	17	1	V → F in AAC61676. Ref.3
	Sequence conflict	274	1	A → G in AAC61676. Ref.3
	Sequence conflict	294	1	A → G in AAC61676. Ref.3
	Sequence conflict	326	1	A → S in AAD17964. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified October 3, 2003. Version 2. Checksum: DCADF445A1437C3C	FASTA	465	49,736
	10 20 30 40 50 60 MKPLLLLVAV ALGLATVVSV VSAGPEAIEC WFVEDAGGGG LSKKPATLLL RHGPRGPPPR 70 80 90 100 110 120 PDLDPKLYFK VDDPAGMLLA AFRRYPAGAS APHCEMSRFI PFPASAKWAR SLSPEQNCPR 130 140 150 160 170 180 ALDGDWLLVS VSSTLFSLSS LLRPQPEPLR EPVVITMATV VLTVLTHNPA PRVQLGKDAV 190 200 210 220 230 240 LDLRFAYAPS ALEGSPSLDA GPPPFGLEWR RQHRGKGHLL LAATPGLAGR MPPAQEKATA 250 260 270 280 290 300 FAAWDDDEPW GPWTGNGTFW LPAVKPSQEG VYLATVHLPY LQGQVSLELT VHKAPRVSLT 310 320 330 340 350 360 PAPVVWAAPG EAPPELLCLA SHFFPAEGLE VKWELRGGPG GSSRKVEGKT WLSTIRHHSD 370 380 390 400 410 420 GSVSQSGHLQ LPPVTAKQHG VHYVCRVYHS SLPASGRSAD VTLEVAGFSG PSIEDGIGLF 430 440 450 460 LSAFLLLGLL KVLGWLAAYW TIPEVSKEKA TAASLTIPRN SKKSQ
	Isoform Short [UniParc]. Checksum: EF81A50547D8A004	FASTA	435	46,621
	10 20 30 40 50 60 MKPLLLLVAV ALGLATVVSV VSAGPEAIEC WFVEDAGGGG LSKKPATLLL RHGPRGPPPR 70 80 90 100 110 120 PDLDPKLYFK VDDPAGMLLA AFRRYPAGAS APHCEMSRFI PFPASAKWAR SLSPEQNCPR 130 140 150 160 170 180 ALDGDWLLVS VSSTLFSLSS LLRPQPEPLR EPVVITMATV VLTVLTHNPA PRVQLGKDAV 190 200 210 220 230 240 LDLRFAYAPS ALEGSPSLDA GPPPFGLEWR RQHRGKGHLL LAATPGLAGR MPPAQEKATA 250 260 270 280 290 300 FAAWDDDEPW GPWTGNGTFW LPAVKPSQEG VYLATVHLPY LQGQVSLELT VHKAPRVSLT 310 320 330 340 350 360 PAPVVWAAPG EAPPELLCLA SHFFPAEGLE VKWELRGGPG GSSRKVEGKT WLSTIRHHSD 370 380 390 400 410 420 GSVSQSGHLQ LPPVTAKQHG VHYVCRVYHS SLPASGRSAD VTLEVAAAYW TIPEVSKEKA 430 TAASLTIPRN SKKSQ

References

[1]	"Peptide-bound major histocompatibility complex class I molecules associate with tapasin before dissociation from transporter associated with antigen processing." Li S., Paulsson K.M., Sjoegren H.-O., Wang P. J. Biol. Chem. 274:8649-8654(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG). Tissue: Liver.
[2]	"Sequence of the mouse major histocomaptibility locus class II region." Rowen L., Qin S., Madan A., Loretz C., Hall J., James R., Dors M., Shaffer T., Abbasi N., Ratcliffe A., Dickhoff R., Lasky S., Hood L. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS LONG AND SHORT). Strain: 129.
[3]	"Sequence, linkage to H2-K, and function of mouse tapasin in MHC class I assembly." Grandea A.G. III, Comber P.G., Wenderfer S.E., Schoenhals G., Frueh K., Monaco J.J., Spies T. Immunogenetics 48:260-265(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
[4]	"Regulation of the expression of mouse TAP-associated glycoprotein (tapasin) by cytokines." Abarca-Heidemann K., Friederichs S., Klamp T., Boehm U., Guethlein L.A., Ortmann B. Immunol. Lett. 83:197-207(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM LONG). Strain: C57BL/10.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF106278 mRNA. Translation: AAD17964.1. AF110520 Genomic DNA. Translation: AAC97973.1. AF100956 Genomic DNA. Translation: AAC69893.1. AF043943 mRNA. Translation: AAC61676.1. AJ316612 Genomic DNA. Translation: CAC69993.1. AJ316613 mRNA. Translation: CAC70721.2.
IPI	IPI00226205. IPI00467062.
RefSeq	NP_033344.1. NM_009318.2.
UniGene	Mm.154457.
3D structure databases
ProteinModelPortal	Q9R233.
SMR	Q9R233. Positions 24-404.
ModBase	Search...
PTM databases
PhosphoSite	Q9R233.
Proteomic databases
PaxDb	Q9R233.
PRIDE	Q9R233.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	21356.
KEGG	mmu:21356.
Organism-specific databases
CTD	6892.
MGI	MGI:1201689. Tapbp.
Phylogenomic databases
eggNOG	NOG41000.
HOGENOM	HOG000231214.
HOVERGEN	HBG005156.
InParanoid	Q9R233.
KO	K08058.
OrthoDB	EOG469QV7.
Enzyme and pathway databases
Reactome	REACT_102124. Immune System.
Gene expression databases
ArrayExpress	Q9R233.
Bgee	Q9R233.
CleanEx	MM_TAPBP.
Genevestigator	Q9R233.
GermOnline	ENSMUSG00000024308. Mus musculus.
Family and domain databases
Gene3D	2.60.40.10. 1 hit.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003006. Ig/MHC_CS. IPR003597. Ig_C1-set. IPR003599. Ig_sub. IPR008056. Tapasin. [Graphical view]
PANTHER	PTHR23411. PTHR23411. 1 hit.
Pfam	PF07654. C1-set. 1 hit. [Graphical view]
PRINTS	PR01669. TAPASIN.
SMART	SM00409. IG. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. PS00290. IG_MHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TAPBP. mouse.
NextBio	300560.
SOURCE	Search...

Entry information

Entry name

TPSN_MOUSE

Accession

Primary (citable) accession number: Q9R233
Secondary accession number(s): O70317 , Q91YE0, Q91YE1, Q9QWT7, Q9Z1W3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	October 3, 2003
Last modified:	April 3, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program