Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9R233 (TPSN_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tapasin

Short name=TPN
Short name=TPSN
Alternative name(s):
TAP-associated protein
TAP-binding protein
Gene names
Name:Tapbp
Synonyms:Tapa
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).

Subunit structure

Interacts with TAP1 and is thus a subunit of the TAP complex. Interaction with TAP1 is TAP2 independent and is required for efficient peptide-TAP interaction. Obligatory mediator for the interaction between newly assembled MHC class I molecules, calreticulin, ERp57 and TAP. Up to 4 MHC class I/tapasin complexes bind to 1 TAP.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type I membrane protein Probable.

Domain

The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminus region By similarity.

Sequence similarities

Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q9R233-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q9R233-2)

The sequence of this isoform differs from the canonical sequence as follows:
     407-436: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 By similarity
Chain24 – 465442Tapasin
PRO_0000014991

Regions

Topological domain24 – 416393Lumenal Potential
Transmembrane417 – 43721Helical; Potential
Topological domain438 – 46528Cytoplasmic Potential
Domain295 – 402108Ig-like C1-type
Compositional bias37 – 404Poly-Gly

Sites

Site4311May be involved in interaction with TAP

Amino acid modifications

Glycosylation2561N-linked (GlcNAc...) Potential
Disulfide bond30 ↔ 94 By similarity
Disulfide bond118Interchain (with C-57 in PDIA3) By similarity
Disulfide bond318 ↔ 385 By similarity

Natural variations

Alternative sequence407 – 43630Missing in isoform Short.
VSP_002578

Experimental info

Sequence conflict171V → F in AAC61676. Ref.3
Sequence conflict2741A → G in AAC61676. Ref.3
Sequence conflict2941A → G in AAC61676. Ref.3
Sequence conflict3261A → S in AAD17964. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified October 3, 2003. Version 2.
Checksum: DCADF445A1437C3C

FASTA46549,736
        10         20         30         40         50         60 
MKPLLLLVAV ALGLATVVSV VSAGPEAIEC WFVEDAGGGG LSKKPATLLL RHGPRGPPPR 

        70         80         90        100        110        120 
PDLDPKLYFK VDDPAGMLLA AFRRYPAGAS APHCEMSRFI PFPASAKWAR SLSPEQNCPR 

       130        140        150        160        170        180 
ALDGDWLLVS VSSTLFSLSS LLRPQPEPLR EPVVITMATV VLTVLTHNPA PRVQLGKDAV 

       190        200        210        220        230        240 
LDLRFAYAPS ALEGSPSLDA GPPPFGLEWR RQHRGKGHLL LAATPGLAGR MPPAQEKATA 

       250        260        270        280        290        300 
FAAWDDDEPW GPWTGNGTFW LPAVKPSQEG VYLATVHLPY LQGQVSLELT VHKAPRVSLT 

       310        320        330        340        350        360 
PAPVVWAAPG EAPPELLCLA SHFFPAEGLE VKWELRGGPG GSSRKVEGKT WLSTIRHHSD 

       370        380        390        400        410        420 
GSVSQSGHLQ LPPVTAKQHG VHYVCRVYHS SLPASGRSAD VTLEVAGFSG PSIEDGIGLF 

       430        440        450        460 
LSAFLLLGLL KVLGWLAAYW TIPEVSKEKA TAASLTIPRN SKKSQ 

« Hide

Isoform Short [UniParc].

Checksum: EF81A50547D8A004
Show »

FASTA43546,621

References

[1]"Peptide-bound major histocompatibility complex class I molecules associate with tapasin before dissociation from transporter associated with antigen processing."
Li S., Paulsson K.M., Sjoegren H.-O., Wang P.
J. Biol. Chem. 274:8649-8654(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
Tissue: Liver.
[2]"Sequence of the mouse major histocomaptibility locus class II region."
Rowen L., Qin S., Madan A., Loretz C., Hall J., James R., Dors M., Shaffer T., Abbasi N., Ratcliffe A., Dickhoff R., Lasky S., Hood L.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS LONG AND SHORT).
Strain: 129.
[3]"Sequence, linkage to H2-K, and function of mouse tapasin in MHC class I assembly."
Grandea A.G. III, Comber P.G., Wenderfer S.E., Schoenhals G., Frueh K., Monaco J.J., Spies T.
Immunogenetics 48:260-265(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
[4]"Regulation of the expression of mouse TAP-associated glycoprotein (tapasin) by cytokines."
Abarca-Heidemann K., Friederichs S., Klamp T., Boehm U., Guethlein L.A., Ortmann B.
Immunol. Lett. 83:197-207(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM LONG).
Strain: C57BL/10.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF106278 mRNA. Translation: AAD17964.1.
AF110520 Genomic DNA. Translation: AAC97973.1.
AF100956 Genomic DNA. Translation: AAC69893.1.
AF043943 mRNA. Translation: AAC61676.1.
AJ316612 Genomic DNA. Translation: CAC69993.1.
AJ316613 mRNA. Translation: CAC70721.2.
RefSeqNP_033344.1. NM_009318.2.
UniGeneMm.154457.

3D structure databases

ProteinModelPortalQ9R233.
SMRQ9R233. Positions 24-404.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9R233. 2 interactions.
MINTMINT-4138357.

PTM databases

PhosphoSiteQ9R233.

Proteomic databases

PaxDbQ9R233.
PRIDEQ9R233.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID21356.
KEGGmmu:21356.
UCSCuc008cae.1. mouse. [Q9R233-1]

Organism-specific databases

CTD6892.
MGIMGI:1201689. Tapbp.

Phylogenomic databases

eggNOGNOG41000.
HOGENOMHOG000231214.
HOVERGENHBG005156.
InParanoidQ9R233.
KOK08058.
PhylomeDBQ9R233.

Enzyme and pathway databases

ReactomeREACT_102124. Immune System.

Gene expression databases

ArrayExpressQ9R233.
BgeeQ9R233.
CleanExMM_TAPBP.
GenevestigatorQ9R233.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR003599. Ig_sub.
IPR008056. Tapasin.
[Graphical view]
PANTHERPTHR23411:SF0. PTHR23411:SF0. 1 hit.
PfamPF07654. C1-set. 1 hit.
[Graphical view]
PRINTSPR01669. TAPASIN.
SMARTSM00409. IG. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTAPBP. mouse.
NextBio300560.
PROQ9R233.
SOURCESearch...

Entry information

Entry nameTPSN_MOUSE
AccessionPrimary (citable) accession number: Q9R233
Secondary accession number(s): O70317 expand/collapse secondary AC list , Q91YE0, Q91YE1, Q9QWT7, Q9Z1W3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: October 3, 2003
Last modified: April 16, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot