Q9R229 (BMP10_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bone morphogenetic protein 10
Short name=BMP-10

Gene names

Name:

Bmp10

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. Ref.4 Ref.5 Ref.6
Subunit structure	Homodimer; disulfide-linked By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	In the embryo, expressed exclusively in the ventricular trabecular myocardium of the developing heart from E9.0-E13.5. By E16.5-E18.5, only detectable in atria. Highly expressed in the adult heart where it is found in the right atrium but not in the left atrium. Lower levels in adult liver and lung. Ref.1 Ref.4 Ref.5
Developmental stage	Down-regulation after E14.5 is critical for cardiomyocytes to undergo normal developmental hypertrophic growth in early postnatal life. Ref.6
Disruption phenotype	Mice die in utero between E9.5 and E10.5. They appear normal at E8.5-E8.75 but display cardiac dysgenesis at E9.0-E9.5 with profound hypoplastic ventricular walls and absence of ventricular trabeculae and have a significantly lower heart rate than wild type embryos. Mutants show up-regulation of Cdkn1c/p57KIP throughout the ventricular wall while levels of Mef2c and Nkx2-5 are normal at E8.5-E8.75 but are down-regulated at E9.25-E9.5. Ref.5
Sequence similarities	Belongs to the TGF-beta family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine Developmental protein Growth factor
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	BMP signaling pathway Inferred from electronic annotation. Source: Compara activin receptor signaling pathway Inferred from electronic annotation. Source: Compara adult heart development Inferred from mutant phenotype Ref.6. Source: BHF-UCL atrial cardiac muscle tissue morphogenesis Inferred from expression pattern Ref.1. Source: BHF-UCL cardiac muscle cell proliferation Inferred from mutant phenotype Ref.5. Source: UniProtKB heart trabecula formation Inferred from mutant phenotype Ref.5. Source: BHF-UCL negative regulation of cardiac muscle hypertrophy Inferred from mutant phenotype Ref.6. Source: BHF-UCL negative regulation of cell growth Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell migration Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of endothelial cell migration Inferred from electronic annotation. Source: Compara pathway-restricted SMAD protein phosphorylation Inferred from direct assay Ref.6. Source: BHF-UCL positive regulation of cardiac muscle cell proliferation Inferred from mutant phenotype PubMed 17336907. Source: BHF-UCL positive regulation of cardiac muscle hypertrophy Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation involved in heart morphogenesis Inferred from genetic interaction PubMed 17336907. Source: BHF-UCL positive regulation of gene expression Inferred from direct assay PubMed 22783020. Source: MGI positive regulation of pathway-restricted SMAD protein phosphorylation Inferred from direct assay Ref.6. Source: BHF-UCL positive regulation of sarcomere organization Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara regulation of cardiac muscle contraction Inferred from electronic annotation. Source: Compara sarcomere organization Inferred from electronic annotation. Source: Compara ventricular cardiac muscle cell development Inferred from direct assay Ref.6. Source: BHF-UCL ventricular cardiac muscle tissue morphogenesis Inferred from mutant phenotype Ref.5PubMed 17336907. Source: BHF-UCL
Cellular_component	Z disc Inferred from electronic annotation. Source: Compara cell surface Inferred from electronic annotation. Source: Compara extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	receptor serine/threonine kinase binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 313	292	Potential		PRO_0000033890
	Chain	314 – 421	108	Bone morphogenetic protein 10		PRO_0000033891
Amino acid modifications
	Glycosylation	67	1	N-linked (GlcNAc...) Potential
	Glycosylation	131	1	N-linked (GlcNAc...) Potential
	Disulfide bond	320 ↔ 386		By similarity
	Disulfide bond	349 ↔ 418		By similarity
	Disulfide bond	353 ↔ 420		By similarity
	Disulfide bond	385		Interchain By similarity
Experimental info
	Sequence conflict	83	1	E → K in AAC95357. Ref.1
	Sequence conflict	83	1	E → K in AAC77461. Ref.2
	Sequence conflict	112	1	Missing in AAC95357. Ref.1
	Sequence conflict	309	1	A → T in AAC95357. Ref.1
	Sequence conflict	309	1	A → T in AAC77461. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9R229 [UniParc]. Last modified November 13, 2007. Version 2. Checksum: 770AF401A64C3F49	FASTA	421	47,888
	10 20 30 40 50 60 MGSLVLPLSA VFCLVAHSAS GSPIMGLEQS PLEEDMPFFD DIFTEQDGID FNTLLQSMKN 70 80 90 100 110 120 EFLKTLNLSD IPVQDTGRVD PPEYMLELYN KFATDRTSMP SANIIRSFKN EDLFSQPVTF 130 140 150 160 170 180 NGLRKYPLLF NVSIPHHEEV VMAELRLYTL VQRDRMMYDG VDRKITIFEV LESADGSEEE 190 200 210 220 230 240 RSMLVLVSTE IYGTNSEWET FDVTDATRRW QKSGPSTHQL EIHIESRQNQ AEDTGRGQLE 250 260 270 280 290 300 IDMSAQNKHD PLLVVFSDDQ SNDKEQKEEL NELITHEQDL DLDSDAFFSG PDEEALLQMR 310 320 330 340 350 360 SNMIDDSSAR IRRNAKGNYC KKTPLYIDFK EIGWDSWIIA PPGYEAYECR GVCNYPLAEH 370 380 390 400 410 420 LTPTKHAIIQ ALVHLKNSQK ASKACCVPTK LDPISILYLD KGVVTYKFKY EGMAVSECGC R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Heart specific expression of mouse BMP-10 a novel member of the TGF-beta superfamily." Neuhaus H., Rosen V., Thies R.S. Mech. Dev. 80:181-184(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: NIH Swiss. Tissue: Heart.
[2]	"Mouse bone morphogenetic protein 10 (BMP-10) genomic sequence, full coding region of exon 2." Celeste A.J. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Nkx2-5 pathways and congenital heart disease: loss of ventricular myocyte lineage specification leads to progressive cardiomyopathy and complete heart block." Pashmforoush M., Lu J.T., Chen H., Amand T.S., Kondo R., Pradervand S., Evans S.M., Clark B., Feramisco J.R., Giles W., Ho S.Y., Benson D.W., Silberbach M., Shou W., Chien K.R. Cell 117:373-386(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[5]	"BMP10 is essential for maintaining cardiac growth during murine cardiogenesis." Chen H., Shi S., Acosta L., Li W., Lu J., Bao S., Chen Z., Yang Z., Schneider M.D., Chien K.R., Conway S.J., Yoder M.C., Haneline L.S., Franco D., Shou W. Development 131:2219-2231(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[6]	"Overexpression of bone morphogenetic protein 10 in myocardium disrupts cardiac postnatal hypertrophic growth." Chen H., Yong W., Ren S., Shen W., He Y., Cox K.A., Zhu W., Li W., Soonpaa M., Payne R.M., Franco D., Field L.J., Rosen V., Wang Y., Shou W. J. Biol. Chem. 281:27481-27491(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF101033 mRNA. Translation: AAC95357.1. AF101440, AF101439 Genomic DNA. Translation: AAC77461.1. BC145983 mRNA. Translation: AAI45984.1.
IPI	IPI00314523.
RefSeq	NP_033886.2. NM_009756.2.
UniGene	Mm.483321.
3D structure databases
ProteinModelPortal	Q9R229.
SMR	Q9R229. Positions 317-421.
ModBase	Search...
PTM databases
PhosphoSite	Q9R229.
Proteomic databases
PaxDb	Q9R229.
PRIDE	Q9R229.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032125; ENSMUSP00000032125; ENSMUSG00000030046.
GeneID	12154.
KEGG	mmu:12154.
UCSC	uc012eol.1. mouse.
Organism-specific databases
CTD	27302.
MGI	MGI:1338820. Bmp10.
Phylogenomic databases
eggNOG	NOG280573.
GeneTree	ENSGT00620000087601.
HOGENOM	HOG000249477.
HOVERGEN	HBG106648.
InParanoid	Q9R229.
KO	K05503.
OMA	YEAYECR.
OrthoDB	EOG40GCQR.
Gene expression databases
Bgee	Q9R229.
CleanEx	MM_BMP10.
Genevestigator	Q9R229.
GermOnline	ENSMUSG00000030046. Mus musculus.
Family and domain databases
InterPro	IPR002405. Inhibin_asu. IPR001839. TGF-b_C. IPR001111. TGF-b_N. IPR015615. TGF-beta-rel. IPR017948. TGFb_CS. [Graphical view]
PANTHER	PTHR11848. PTHR11848. 1 hit.
Pfam	PF00019. TGF_beta. 1 hit. PF00688. TGFb_propeptide. 1 hit. [Graphical view]
PRINTS	PR00669. INHIBINA.
SMART	SM00204. TGFB. 1 hit. [Graphical view]
PROSITE	PS00250. TGF_BETA_1. 1 hit. PS51362. TGF_BETA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	280491.
SOURCE	Search...

Entry information

Entry name

BMP10_MOUSE

Accession

Primary (citable) accession number: Q9R229
Secondary accession number(s): A6H6S5, Q9Z1V8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 13, 2007
Last modified:	April 3, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program