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Protein

Bone morphogenetic protein 10

Gene

Bmp10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. May reduce cell migration and cell matrix adhesion in breast cancer cell lines (By similarity).By similarity

GO - Molecular functioni

  1. cytokine activity Source: GO_Central
  2. hormone activity Source: MGI
  3. receptor serine/threonine kinase binding Source: UniProtKB
  4. telethonin binding Source: MGI
  5. transforming growth factor beta receptor binding Source: GO_Central

GO - Biological processi

  1. activin receptor signaling pathway Source: MGI
  2. adult heart development Source: BHF-UCL
  3. atrial cardiac muscle tissue morphogenesis Source: BHF-UCL
  4. BMP signaling pathway Source: MGI
  5. cardiac muscle cell proliferation Source: UniProtKB
  6. cell adhesion Source: UniProtKB-KW
  7. heart development Source: MGI
  8. heart trabecula formation Source: BHF-UCL
  9. negative regulation of cardiac muscle hypertrophy Source: BHF-UCL
  10. negative regulation of cell growth Source: UniProtKB
  11. negative regulation of cell migration Source: UniProtKB
  12. negative regulation of endothelial cell migration Source: MGI
  13. pathway-restricted SMAD protein phosphorylation Source: BHF-UCL
  14. positive regulation of cardiac muscle cell proliferation Source: BHF-UCL
  15. positive regulation of cardiac muscle hypertrophy Source: MGI
  16. positive regulation of cell proliferation involved in heart morphogenesis Source: BHF-UCL
  17. positive regulation of gene expression Source: MGI
  18. positive regulation of pathway-restricted SMAD protein phosphorylation Source: BHF-UCL
  19. positive regulation of sarcomere organization Source: MGI
  20. positive regulation of transcription, DNA-templated Source: MGI
  21. regulation of apoptotic process Source: GO_Central
  22. regulation of cardiac muscle contraction Source: MGI
  23. regulation of cardiac muscle hypertrophy in response to stress Source: MGI
  24. regulation of MAPK cascade Source: GO_Central
  25. sarcomere organization Source: Ensembl
  26. SMAD protein signal transduction Source: GO_Central
  27. ventricular cardiac muscle cell development Source: BHF-UCL
  28. ventricular cardiac muscle tissue morphogenesis Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Developmental protein, Growth factor

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiREACT_332220. Molecules associated with elastic fibres.

Names & Taxonomyi

Protein namesi
Recommended name:
Bone morphogenetic protein 10
Short name:
BMP-10
Gene namesi
Name:Bmp10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1338820. Bmp10.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. cell surface Source: MGI
  2. cytoplasm Source: MGI
  3. extracellular space Source: MGI
  4. Z disc Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Mice die in utero between E9.5 and E10.5. They appear normal at E8.5-E8.75 but display cardiac dysgenesis at E9.0-E9.5 with profound hypoplastic ventricular walls and absence of ventricular trabeculae and have a significantly lower heart rate than wild type embryos. Mutants show up-regulation of Cdkn1c/p57KIP throughout the ventricular wall while levels of Mef2c and Nkx2-5 are normal at E8.5-E8.75 but are down-regulated at E9.25-E9.5.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Propeptidei22 – 313292Sequence AnalysisPRO_0000033890Add
BLAST
Chaini314 – 421108Bone morphogenetic protein 10PRO_0000033891Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi67 – 671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi320 ↔ 386By similarity
Disulfide bondi349 ↔ 418By similarity
Disulfide bondi353 ↔ 420By similarity
Disulfide bondi385 – 385InterchainBy similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9R229.
PRIDEiQ9R229.

PTM databases

PhosphoSiteiQ9R229.

Expressioni

Tissue specificityi

In the embryo, expressed exclusively in the ventricular trabecular myocardium of the developing heart from E9.0-E13.5. By E16.5-E18.5, only detectable in atria. Highly expressed in the adult heart where it is found in the right atrium but not in the left atrium. Lower levels in adult liver and lung.3 Publications

Developmental stagei

Down-regulation after E14.5 is critical for cardiomyocytes to undergo normal developmental hypertrophic growth in early postnatal life.1 Publication

Gene expression databases

BgeeiQ9R229.
CleanExiMM_BMP10.
GenevestigatoriQ9R229.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

IntActiQ9R229. 1 interaction.
MINTiMINT-4089291.

Structurei

3D structure databases

ProteinModelPortaliQ9R229.
SMRiQ9R229. Positions 317-421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TGF-beta family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG280573.
GeneTreeiENSGT00760000118883.
HOGENOMiHOG000249477.
HOVERGENiHBG106648.
InParanoidiQ9R229.
KOiK05503.
OMAiNVSIPHH.
OrthoDBiEOG7ZD1V3.
PhylomeDBiQ9R229.
TreeFamiTF316134.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR002405. Inhibin_asu.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 1 hit.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
PRINTSiPR00669. INHIBINA.
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R229-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSLVLPLSA VFCLVAHSAS GSPIMGLEQS PLEEDMPFFD DIFTEQDGID
60 70 80 90 100
FNTLLQSMKN EFLKTLNLSD IPVQDTGRVD PPEYMLELYN KFATDRTSMP
110 120 130 140 150
SANIIRSFKN EDLFSQPVTF NGLRKYPLLF NVSIPHHEEV VMAELRLYTL
160 170 180 190 200
VQRDRMMYDG VDRKITIFEV LESADGSEEE RSMLVLVSTE IYGTNSEWET
210 220 230 240 250
FDVTDATRRW QKSGPSTHQL EIHIESRQNQ AEDTGRGQLE IDMSAQNKHD
260 270 280 290 300
PLLVVFSDDQ SNDKEQKEEL NELITHEQDL DLDSDAFFSG PDEEALLQMR
310 320 330 340 350
SNMIDDSSAR IRRNAKGNYC KKTPLYIDFK EIGWDSWIIA PPGYEAYECR
360 370 380 390 400
GVCNYPLAEH LTPTKHAIIQ ALVHLKNSQK ASKACCVPTK LDPISILYLD
410 420
KGVVTYKFKY EGMAVSECGC R
Length:421
Mass (Da):47,888
Last modified:November 13, 2007 - v2
Checksum:i770AF401A64C3F49
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti83 – 831E → K in AAC95357 (PubMed:10072785).Curated
Sequence conflicti83 – 831E → K in AAC77461 (Ref. 2) Curated
Sequence conflicti112 – 1121Missing in AAC95357 (PubMed:10072785).Curated
Sequence conflicti309 – 3091A → T in AAC95357 (PubMed:10072785).Curated
Sequence conflicti309 – 3091A → T in AAC77461 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF101033 mRNA. Translation: AAC95357.1.
AF101440, AF101439 Genomic DNA. Translation: AAC77461.1.
BC145983 mRNA. Translation: AAI45984.1.
CCDSiCCDS51838.1.
RefSeqiNP_033886.2. NM_009756.2.
UniGeneiMm.483321.

Genome annotation databases

EnsembliENSMUST00000032125; ENSMUSP00000032125; ENSMUSG00000030046.
GeneIDi12154.
KEGGimmu:12154.
UCSCiuc012eol.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF101033 mRNA. Translation: AAC95357.1.
AF101440, AF101439 Genomic DNA. Translation: AAC77461.1.
BC145983 mRNA. Translation: AAI45984.1.
CCDSiCCDS51838.1.
RefSeqiNP_033886.2. NM_009756.2.
UniGeneiMm.483321.

3D structure databases

ProteinModelPortaliQ9R229.
SMRiQ9R229. Positions 317-421.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9R229. 1 interaction.
MINTiMINT-4089291.

PTM databases

PhosphoSiteiQ9R229.

Proteomic databases

PaxDbiQ9R229.
PRIDEiQ9R229.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032125; ENSMUSP00000032125; ENSMUSG00000030046.
GeneIDi12154.
KEGGimmu:12154.
UCSCiuc012eol.1. mouse.

Organism-specific databases

CTDi27302.
MGIiMGI:1338820. Bmp10.

Phylogenomic databases

eggNOGiNOG280573.
GeneTreeiENSGT00760000118883.
HOGENOMiHOG000249477.
HOVERGENiHBG106648.
InParanoidiQ9R229.
KOiK05503.
OMAiNVSIPHH.
OrthoDBiEOG7ZD1V3.
PhylomeDBiQ9R229.
TreeFamiTF316134.

Enzyme and pathway databases

ReactomeiREACT_332220. Molecules associated with elastic fibres.

Miscellaneous databases

NextBioi280491.
PROiQ9R229.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R229.
CleanExiMM_BMP10.
GenevestigatoriQ9R229.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR002405. Inhibin_asu.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 1 hit.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
PRINTSiPR00669. INHIBINA.
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Heart specific expression of mouse BMP-10 a novel member of the TGF-beta superfamily."
    Neuhaus H., Rosen V., Thies R.S.
    Mech. Dev. 80:181-184(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: NIH Swiss.
    Tissue: Heart.
  2. "Mouse bone morphogenetic protein 10 (BMP-10) genomic sequence, full coding region of exon 2."
    Celeste A.J.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Nkx2-5 pathways and congenital heart disease: loss of ventricular myocyte lineage specification leads to progressive cardiomyopathy and complete heart block."
    Pashmforoush M., Lu J.T., Chen H., Amand T.S., Kondo R., Pradervand S., Evans S.M., Clark B., Feramisco J.R., Giles W., Ho S.Y., Benson D.W., Silberbach M., Shou W., Chien K.R.
    Cell 117:373-386(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  5. Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  6. "Overexpression of bone morphogenetic protein 10 in myocardium disrupts cardiac postnatal hypertrophic growth."
    Chen H., Yong W., Ren S., Shen W., He Y., Cox K.A., Zhu W., Li W., Soonpaa M., Payne R.M., Franco D., Field L.J., Rosen V., Wang Y., Shou W.
    J. Biol. Chem. 281:27481-27491(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiBMP10_MOUSE
AccessioniPrimary (citable) accession number: Q9R229
Secondary accession number(s): A6H6S5, Q9Z1V8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 13, 2007
Last modified: April 1, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.