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Q9R229 (BMP10_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bone morphogenetic protein 10

Short name=BMP-10
Gene names
Name:Bmp10
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length421 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. May reduce cell migration and cell matrix adhesion in breast cancer cell lines By similarity. Ref.4 Ref.5 Ref.6

Subunit structure

Homodimer; disulfide-linked By similarity.

Subcellular location

Secreted By similarity.

Tissue specificity

In the embryo, expressed exclusively in the ventricular trabecular myocardium of the developing heart from E9.0-E13.5. By E16.5-E18.5, only detectable in atria. Highly expressed in the adult heart where it is found in the right atrium but not in the left atrium. Lower levels in adult liver and lung. Ref.1 Ref.4 Ref.5

Developmental stage

Down-regulation after E14.5 is critical for cardiomyocytes to undergo normal developmental hypertrophic growth in early postnatal life. Ref.6

Disruption phenotype

Mice die in utero between E9.5 and E10.5. They appear normal at E8.5-E8.75 but display cardiac dysgenesis at E9.0-E9.5 with profound hypoplastic ventricular walls and absence of ventricular trabeculae and have a significantly lower heart rate than wild type embryos. Mutants show up-regulation of Cdkn1c/p57KIP throughout the ventricular wall while levels of Mef2c and Nkx2-5 are normal at E8.5-E8.75 but are down-regulated at E9.25-E9.5. Ref.5

Sequence similarities

Belongs to the TGF-beta family.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentSecreted
   DomainSignal
   Molecular functionCytokine
Developmental protein
Growth factor
   PTMCleavage on pair of basic residues
Disulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processBMP signaling pathway

Inferred from electronic annotation. Source: Ensembl

activin receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

adult heart development

Inferred from mutant phenotype Ref.6. Source: BHF-UCL

atrial cardiac muscle tissue morphogenesis

Inferred from expression pattern Ref.1. Source: BHF-UCL

cardiac muscle cell proliferation

Inferred from mutant phenotype Ref.5. Source: UniProtKB

cell adhesion

Inferred from electronic annotation. Source: UniProtKB-KW

heart development

Inferred from mutant phenotype Ref.5. Source: MGI

heart trabecula formation

Inferred from mutant phenotype Ref.5. Source: BHF-UCL

negative regulation of cardiac muscle hypertrophy

Inferred from mutant phenotype Ref.6. Source: BHF-UCL

negative regulation of cell growth

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of cell migration

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of endothelial cell migration

Inferred from electronic annotation. Source: Ensembl

pathway-restricted SMAD protein phosphorylation

Inferred from direct assay Ref.6. Source: BHF-UCL

positive regulation of cardiac muscle cell proliferation

Inferred from mutant phenotype PubMed 17336907. Source: BHF-UCL

positive regulation of cardiac muscle hypertrophy

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation involved in heart morphogenesis

Inferred from genetic interaction PubMed 17336907. Source: BHF-UCL

positive regulation of gene expression

Inferred from direct assay PubMed 22783020. Source: MGI

positive regulation of pathway-restricted SMAD protein phosphorylation

Inferred from direct assay Ref.6. Source: BHF-UCL

positive regulation of sarcomere organization

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

regulation of cardiac muscle contraction

Inferred from electronic annotation. Source: Ensembl

sarcomere organization

Inferred from electronic annotation. Source: Ensembl

ventricular cardiac muscle cell development

Inferred from direct assay Ref.6. Source: BHF-UCL

ventricular cardiac muscle tissue morphogenesis

Inferred from mutant phenotype Ref.5PubMed 17336907. Source: BHF-UCL

   Cellular_componentZ disc

Inferred from electronic annotation. Source: Ensembl

cell surface

Inferred from electronic annotation. Source: Ensembl

extracellular space

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionreceptor serine/threonine kinase binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Propeptide22 – 313292 Potential
PRO_0000033890
Chain314 – 421108Bone morphogenetic protein 10
PRO_0000033891

Amino acid modifications

Glycosylation671N-linked (GlcNAc...) Potential
Glycosylation1311N-linked (GlcNAc...) Potential
Disulfide bond320 ↔ 386 By similarity
Disulfide bond349 ↔ 418 By similarity
Disulfide bond353 ↔ 420 By similarity
Disulfide bond385Interchain By similarity

Experimental info

Sequence conflict831E → K in AAC95357. Ref.1
Sequence conflict831E → K in AAC77461. Ref.2
Sequence conflict1121Missing in AAC95357. Ref.1
Sequence conflict3091A → T in AAC95357. Ref.1
Sequence conflict3091A → T in AAC77461. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9R229 [UniParc].

Last modified November 13, 2007. Version 2.
Checksum: 770AF401A64C3F49

FASTA42147,888
        10         20         30         40         50         60 
MGSLVLPLSA VFCLVAHSAS GSPIMGLEQS PLEEDMPFFD DIFTEQDGID FNTLLQSMKN 

        70         80         90        100        110        120 
EFLKTLNLSD IPVQDTGRVD PPEYMLELYN KFATDRTSMP SANIIRSFKN EDLFSQPVTF 

       130        140        150        160        170        180 
NGLRKYPLLF NVSIPHHEEV VMAELRLYTL VQRDRMMYDG VDRKITIFEV LESADGSEEE 

       190        200        210        220        230        240 
RSMLVLVSTE IYGTNSEWET FDVTDATRRW QKSGPSTHQL EIHIESRQNQ AEDTGRGQLE 

       250        260        270        280        290        300 
IDMSAQNKHD PLLVVFSDDQ SNDKEQKEEL NELITHEQDL DLDSDAFFSG PDEEALLQMR 

       310        320        330        340        350        360 
SNMIDDSSAR IRRNAKGNYC KKTPLYIDFK EIGWDSWIIA PPGYEAYECR GVCNYPLAEH 

       370        380        390        400        410        420 
LTPTKHAIIQ ALVHLKNSQK ASKACCVPTK LDPISILYLD KGVVTYKFKY EGMAVSECGC 


R 

« Hide

References

« Hide 'large scale' references
[1]"Heart specific expression of mouse BMP-10 a novel member of the TGF-beta superfamily."
Neuhaus H., Rosen V., Thies R.S.
Mech. Dev. 80:181-184(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: NIH Swiss.
Tissue: Heart.
[2]"Mouse bone morphogenetic protein 10 (BMP-10) genomic sequence, full coding region of exon 2."
Celeste A.J.
Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Nkx2-5 pathways and congenital heart disease: loss of ventricular myocyte lineage specification leads to progressive cardiomyopathy and complete heart block."
Pashmforoush M., Lu J.T., Chen H., Amand T.S., Kondo R., Pradervand S., Evans S.M., Clark B., Feramisco J.R., Giles W., Ho S.Y., Benson D.W., Silberbach M., Shou W., Chien K.R.
Cell 117:373-386(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[5]"BMP10 is essential for maintaining cardiac growth during murine cardiogenesis."
Chen H., Shi S., Acosta L., Li W., Lu J., Bao S., Chen Z., Yang Z., Schneider M.D., Chien K.R., Conway S.J., Yoder M.C., Haneline L.S., Franco D., Shou W.
Development 131:2219-2231(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[6]"Overexpression of bone morphogenetic protein 10 in myocardium disrupts cardiac postnatal hypertrophic growth."
Chen H., Yong W., Ren S., Shen W., He Y., Cox K.A., Zhu W., Li W., Soonpaa M., Payne R.M., Franco D., Field L.J., Rosen V., Wang Y., Shou W.
J. Biol. Chem. 281:27481-27491(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF101033 mRNA. Translation: AAC95357.1.
AF101440, AF101439 Genomic DNA. Translation: AAC77461.1.
BC145983 mRNA. Translation: AAI45984.1.
CCDSCCDS51838.1.
RefSeqNP_033886.2. NM_009756.2.
UniGeneMm.483321.

3D structure databases

ProteinModelPortalQ9R229.
SMRQ9R229. Positions 317-421.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9R229. 1 interaction.
MINTMINT-4089291.

PTM databases

PhosphoSiteQ9R229.

Proteomic databases

PaxDbQ9R229.
PRIDEQ9R229.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032125; ENSMUSP00000032125; ENSMUSG00000030046.
GeneID12154.
KEGGmmu:12154.
UCSCuc012eol.1. mouse.

Organism-specific databases

CTD27302.
MGIMGI:1338820. Bmp10.

Phylogenomic databases

eggNOGNOG280573.
GeneTreeENSGT00740000115456.
HOGENOMHOG000249477.
HOVERGENHBG106648.
InParanoidQ9R229.
KOK05503.
OMANVSIPHH.
OrthoDBEOG7ZD1V3.
PhylomeDBQ9R229.
TreeFamTF316134.

Gene expression databases

BgeeQ9R229.
CleanExMM_BMP10.
GenevestigatorQ9R229.

Family and domain databases

Gene3D2.10.90.10. 1 hit.
InterProIPR029034. Cystine-knot_cytokine.
IPR002405. Inhibin_asu.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERPTHR11848. PTHR11848. 1 hit.
PfamPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
PRINTSPR00669. INHIBINA.
SMARTSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMSSF57501. SSF57501. 1 hit.
PROSITEPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio280491.
PROQ9R229.
SOURCESearch...

Entry information

Entry nameBMP10_MOUSE
AccessionPrimary (citable) accession number: Q9R229
Secondary accession number(s): A6H6S5, Q9Z1V8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 13, 2007
Last modified: July 9, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot