Q9R226 (KHDR3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
KH domain-containing, RNA-binding, signal transduction-associated protein 3

Alternative name(s):
RNA-binding protein Etoile
Sam68-like mammalian protein 2
Short name=SLM-2

Gene names

Name:	Khdrbs3
Synonyms:	Salp, Slm2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. May play a role as a negative regulator of cell growth. Inhibits cell proliferation. Involved in splice site selection of vascular endothelial growth factor. Induces an increased concentration-dependent incorporation of exon in CD44 pre-mRNA by direct binding to purine-rich exonic enhancer. RNA-binding abilities are down-regulated by tyrosine kinase PTK6. Binds preferentially to the 5'-[AU]UAAA-3' motif in vitro. Ref.4
Subunit structure	Self-associates to form homooligomers. Interacts with the splicing regulatory proteins SFRS9, SAFB and YTHDC1. Interacts also with HNRPL and SLM1/KHDRBS2. Interacts with KHDRBS1, RBMX, RBMY1A1 and with p85 subunit of PI3-kinase By similarity.
Subcellular location	Nucleus. Note: Localized in a compartment adjacent to the nucleolus, but distinct from the peri-nucleolar one. Ref.1 Ref.4
Tissue specificity	Highly expressed in testis. Ref.1 Ref.4
Domain	The proline-rich site binds the SH3 domain of the p85 subunit of PI3-kinase.
Post-translational modification	Phosphorylated on tyrosine residues by PTK6. Ref.4
Sequence similarities	Belongs to the KHDRBS family. Contains 1 KH domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	SH3-binding
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	SH3 domain binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	KH domain-containing, RNA-binding, signal transduction-associated protein 3		PRO_0000232523
Regions
	Domain	61 – 127	67	KH
	Compositional bias	250 – 261	12	Pro-rich
	Compositional bias	266 – 316	51	Tyr-rich
Amino acid modifications
	Modified residue	5	1	Phosphotyrosine By similarity
Experimental info
	Mutagenesis	215 – 217	3	AVG → GVV: Confers SIAH1-mediated degradation and strong SIAH1 binding. Ref.3
	Sequence conflict	160	1	N → Y in AAC31753. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9R226 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F061C85FC698FF40	FASTA	346	38,807
	10 20 30 40 50 60 MEEKYLPELM AEKDSLDPSF THALRLVNRE IEKFQKGEGK EEEKYIDVVI NKNMKLGQKV 70 80 90 100 110 120 LIPVKQFPKF NFVGKLLGPR GNSLKRLQEE TLTKMSILGK GSMRDKAKEE ELRKSGEAKY 130 140 150 160 170 180 FHLNDDLHVL IEVFAPPAEA YARMGHALEE IKKFLIPDYN DEIRQAQLQE LTYLNGGSEN 190 200 210 220 230 240 ADVPVVRGKS TLRTRGVTTP AITRGRGGVT ARPVAVGVPR GTPTPRGVLS TRGPVSRGRG 250 260 270 280 290 300 LLTPRARGVP PTGYRPPPPP PTQETYGEYD YDDGYGTAYD EQSYDSYDNS YSTPAQSAAD 310 320 330 340 YYDYGHGLSE DAYDSYGQEE WTNSRHKAPS ARTAKGVYRD QPYGRY

References

[1]	"T-STAR/ETOILE: a novel relative of SAM68 that interacts with an RNA-binding protein implicated in spermatogenesis." Venables J.P., Vernet C., Chew S.L., Elliott D.J., Cowmeadow R.B., Wu J., Cooke H.J., Artzt K., Eperon I.C. Hum. Mol. Genet. 8:959-969(1999) [PubMed: 10332027] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[2]	"Characterization of Sam68-like mammalian proteins SLM-1 and SLM-2: SLM-1 is a Src substrate during mitosis." Di Fruscio M., Chen T., Richard S. Proc. Natl. Acad. Sci. U.S.A. 96:2710-2715(1999) [PubMed: 10077576] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"SIAH1 targets the alternative splicing factor T-STAR for degradation by the proteasome." Venables J.P., Dalgliesh C., Paronetto M.P., Skitt L., Thornton J.K., Saunders P.T., Sette C., Jones K.T., Elliott D.J. Hum. Mol. Genet. 13:1525-1534(2004) [PubMed: 15163637] [Abstract] Cited for: MUTAGENESIS OF 215-ALA--GLY-217.
[4]	"The nuclear tyrosine kinase BRK/Sik phosphorylates and inhibits the RNA-binding activities of the Sam68-like mammalian proteins SLM-1 and SLM-2." Haegebarth A., Heap D., Bie W., Derry J.J., Richard S., Tyner A.L. J. Biol. Chem. 279:54398-54404(2004) [PubMed: 15471878] [Abstract] Cited for: PHOSPHORYLATION BY PTK6, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF079763 mRNA. Translation: AAC31753.1. AF099092 mRNA. Translation: AAC72396.1. BC031507 mRNA. Translation: AAH31507.1. BC057577 mRNA. Translation: AAH57577.1.
IPI	IPI00314520.
RefSeq	NP_034288.2. NM_010158.2.
UniGene	Mm.17964.
3D structure databases
ProteinModelPortal	Q9R226.
SMR	Q9R226. Positions 2-37, 55-185.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9R226. 1 interaction.
STRING	Q9R226.
PTM databases
PhosphoSite	Q9R226.
Proteomic databases
PRIDE	Q9R226.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022954; ENSMUSP00000022954; ENSMUSG00000022332.
GeneID	13992.
KEGG	mmu:13992.
UCSC	uc007wbf.1. mouse.
Organism-specific databases
CTD	10656.
MGI	MGI:1313312. Khdrbs3.
Phylogenomic databases
eggNOG	roNOG08706.
GeneTree	ENSGT00550000074434.
HOGENOM	HBG713086.
HOVERGEN	HBG079164.
InParanoid	Q9R226.
OMA	GEYEYDD.
OrthoDB	EOG4DBTFN.
PhylomeDB	Q9R226.
Gene expression databases
ArrayExpress	Q9R226.
Bgee	Q9R226.
Genevestigator	Q9R226.
GermOnline	ENSMUSG00000022332. Mus musculus.
Family and domain databases
InterPro	IPR004087. KH. [Graphical view]
KO	K14942.
SMART	SM00322. KH. 1 hit. [Graphical view]
PROSITE	PS50084. KH_TYPE_1. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	284872.
SOURCE	Search...

Entry information

Entry name

KHDR3_MOUSE

Accession

Primary (citable) accession number: Q9R226
Secondary accession number(s): O88624

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	May 1, 2000
Last modified:	November 16, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program