Transcription factor EB - Mus musculus (Mouse)

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Q9R210 (TFEB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 86. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Transcription factor EB

Gene names

Name:	Tfeb
Synonyms:	Tcfeb

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4⁺ T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Ref.2 Ref.3
Subunit structure	Homodimer and heterodimer; with TFE3 or MITF By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Mainly present in the cytoplasm. Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus By similarity.
Tissue specificity	Widely expressed. Ref.3
Developmental stage	Expressed at low levels in the embryo but at high levels in the labyrinthine trophoblast cells of the placenta. Ref.2
Domain	The leucin zipper region is essential for homo- or heterodimerization and high-affinity DNA binding. DNA binding is mediated by the basic region By similarity.
Post-translational modification	Sumoylated; does not affect dimerization with MITF By similarity.
Disruption phenotype	Death between 9.5 and 10.5 days in embryonic development. Embryos display severe defects in placental vascularization. The embryonic vasculature forms normally but few vessels are seen entering the placenta and those that do enter fail to thrive and branch normally. Ref.2
Sequence similarities	Belongs to the MiT/TFE family. Contains 1 basic helix-loop-helix (bHLH) domain.
Sequence caution	The sequence AAD20979.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Adaptive immunity Immunity Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	embryonic placenta development Inferred from mutant phenotype Ref.2. Source: UniProtKB humoral immune response Inferred from mutant phenotype Ref.3. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.3. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB transcription factor complex Inferred from direct assay. Source: MGI
Molecular function	protein heterodimerization activity Inferred from physical interaction Ref.3. Source: UniProtKB sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.3. Source: UniProtKB transcription regulatory region DNA binding Inferred from direct assay Ref.3. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 475

475

Transcription factor EB

PRO_0000127474

Regions

Domain

248 – 288

Helix-loop-helix motif

Domain

297 – 318

Leucine-zipper

DNA binding

232 – 247

Basic motif Ref.3

Region

155 – 164

Strong transcription activation domain Potential

Compositional bias

10 – 43

Gln-rich

Compositional bias

35 – 43

Poly-Gln

Compositional bias

363 – 413

Pro-rich

Amino acid modifications

Modified residue

108

Phosphoserine By similarity

Modified residue

113

Phosphoserine Ref.5

Modified residue

121

Phosphoserine Ref.5 Ref.6

Modified residue

133

Phosphoserine By similarity

Modified residue

137

Phosphoserine By similarity

Modified residue

141

Phosphoserine By similarity

Modified residue

466

Phosphoserine Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

Q9R210 [UniParc].

Last modified August 29, 2001. Version 2.
Checksum: 4249C6F501968326
Show »

FASTA

475

52,614

        10         20         30         40         50         60 
MASRIGLRMQ LMREQAQQEE QRERMQQQAV MHYMQQQQQQ QQQLGGPPTP AINTPVHFQS 

        70         80         90        100        110        120 
PPPVPGEVLK VQSYLENPTS YHLQQSQHQK VREYLSETYG NKFAAHVSPA QGSPKPAPAA 

       130        140        150        160        170        180 
SPGVRAGHVL STSAGNSAPN SPMAMLHISS NPEKEFDDVI DNIMRLDSVL GYINPEMQMP 

       190        200        210        220        230        240 
NTLPLSSSHL NVYSGDPQVT ASMVGVTSSS CPADLTQKRE LTDAESRALA KERQKKDNHN 

       250        260        270        280        290        300 
LIERRRRFNI NDRIKELGML IPKANDLDVR WNKGTILKAS VDYIRRMQKD LQKSRELENH 

       310        320        330        340        350        360 
SRRLEMTNKQ LWLRIQELEM QARVHGLPTT SPSGVNMAEL AQQVVKQELP SEDGPGEALM 

       370        380        390        400        410        420 
LGPEVPEPEQ MPALPPQAPL PSAAQPQSPF HHLDFSHGLS FGGGGDEGPT GYPDTLGTEH 

       430        440        450        460        470 
GSPFPNLSKK DLDLMLLDDS LLPLASDPLF STMSPEASKA SSRRSSFSME EGDVL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the murine genes for bHLH-ZIP transcription factors TFEC and TFEB reveal a common gene organization for all MiT subfamily members." Rehli M., Den Elzen N., Cassady A.I., Ostrowski M.C., Hume D.A. Genomics 56:111-120(1999) [PubMed: 10036191] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"The bHLH-Zip transcription factor Tfeb is essential for placental vascularization." Steingrimsson E., Tessarollo L., Reid S.W., Jenkins N.A., Copeland N.G. Development 125:4607-4616(1998) [PubMed: 9806910] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
[3]	"Transcription factors TFE3 and TFEB are critical for CD40 ligand expression and thymus-dependent humoral immunity." Huan C., Kelly M.L., Steele R., Shapira I., Gottesman S.R.S., Roman C.A.J. Nat. Immunol. 7:1082-1091(2006) [PubMed: 16936731] [Abstract] Cited for: FUNCTION, DNA-BINDING, TISSUE SPECIFICITY.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-466, MASS SPECTROMETRY. Tissue: Liver.
[5]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 19367708] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-113 AND SER-121, MASS SPECTROMETRY. Tissue: Melanoma.
[6]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-121, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF079095 mRNA. Translation: AAD20979.1. Different initiation.
IPI	IPI00314502.
RefSeq	NP_001155194.1. NM_001161722.1. NP_001155195.1. NM_001161723.1. NP_035679.3. NM_011549.3.
UniGene	Mm.2305.
3D structure databases
ProteinModelPortal	Q9R210.
SMR	Q9R210. Positions 232-296.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9R210. 1 interaction.
STRING	Q9R210.
PTM databases
PhosphoSite	Q9R210.
Proteomic databases
PRIDE	Q9R210.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000086932; ENSMUSP00000084151; ENSMUSG00000023990. ENSMUST00000113288; ENSMUSP00000108913; ENSMUSG00000023990.
GeneID	21425.
KEGG	mmu:21425.
Organism-specific databases
CTD	7942.
MGI	MGI:103270. Tfeb.
Phylogenomic databases
GeneTree	ENSGT00390000004402.
HOVERGEN	HBG006768.
InParanoid	Q9R210.
OrthoDB	EOG469QTV.
Gene expression databases
ArrayExpress	Q9R210.
Bgee	Q9R210.
CleanEx	MM_TCFEB.
Genevestigator	Q9R210.
GermOnline	ENSMUSG00000023990. Mus musculus.
Family and domain databases
InterPro	IPR024097. bHLH_ZIP_TF. IPR021802. bHLH_ZIP_TF_MiT/TFE. IPR011598. HLH_DNA-bd. IPR024098. TFEB. [Graphical view]
Gene3D	G3DSA:4.10.280.10. HLH_DNA_bd. 1 hit.
KO	K15590.
PANTHER	PTHR10014. PTHR10014. 1 hit. PTHR10014:SF9. PTHR10014:SF9. 1 hit.
Pfam	PF11851. DUF3371. 1 hit. PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. HLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

TFEB_MOUSE

Accession

Primary (citable) accession number: Q9R210

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	August 29, 2001
Last modified:	January 25, 2012
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents