Reviewed, UniProtKB/Swiss-Prot Q9R210 (TFEB_MOUSE)

Names and origin

Protein names

Recommended name:
    Transcription factor EB

Gene names

Name:	Tfeb
Synonyms:	Tcfeb

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable transcription factor binds to the USF/MLTF site and probably recognizes E-box sequences in the heavy-chain immunoglobulin enhancer By similarity.
Subunit structure	Efficient DNA binding requires dimerization with another bHLH protein By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the MiT/TFE family. Contains 1 basic helix-loop-helix (bHLH) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	positive regulation of transcription, DNA-dependent Inferred from direct assay. Source: MGI transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	transcription factor complex Inferred from direct assay. Source: MGI
Molecular function	transcription factor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	Transcription factor EB		PRO_0000127474
Regions
	Domain	248 – 288	41	Helix-loop-helix motif
	Domain	297 – 318	22	Leucine-zipper Potential
	DNA binding	232 – 247	16	Basic motif
	Region	155 – 164	10	Strong transcription activation domain Potential
	Compositional bias	10 – 43	34	Gln-rich
	Compositional bias	35 – 43	9	Poly-Gln
	Compositional bias	363 – 413	51	Pro-rich
Amino acid modifications
	Modified residue	108	1	Phosphoserine By similarity
	Modified residue	113	1	Phosphoserine By similarity
	Modified residue	121	1	Phosphoserine
	Modified residue	133	1	Phosphoserine By similarity
	Modified residue	137	1	Phosphoserine By similarity
	Modified residue	141	1	Phosphoserine By similarity
	Modified residue	466	1	Phosphoserine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9R210-1 [UniParc]. Last modified August 29, 2001. Version 2. Checksum: 4249C6F501968326	FASTA	475	52,614
	10 20 30 40 50 60 MASRIGLRMQ LMREQAQQEE QRERMQQQAV MHYMQQQQQQ QQQLGGPPTP AINTPVHFQS 70 80 90 100 110 120 PPPVPGEVLK VQSYLENPTS YHLQQSQHQK VREYLSETYG NKFAAHVSPA QGSPKPAPAA 130 140 150 160 170 180 SPGVRAGHVL STSAGNSAPN SPMAMLHISS NPEKEFDDVI DNIMRLDSVL GYINPEMQMP 190 200 210 220 230 240 NTLPLSSSHL NVYSGDPQVT ASMVGVTSSS CPADLTQKRE LTDAESRALA KERQKKDNHN 250 260 270 280 290 300 LIERRRRFNI NDRIKELGML IPKANDLDVR WNKGTILKAS VDYIRRMQKD LQKSRELENH 310 320 330 340 350 360 SRRLEMTNKQ LWLRIQELEM QARVHGLPTT SPSGVNMAEL AQQVVKQELP SEDGPGEALM 370 380 390 400 410 420 LGPEVPEPEQ MPALPPQAPL PSAAQPQSPF HHLDFSHGLS FGGGGDEGPT GYPDTLGTEH 430 440 450 460 470 GSPFPNLSKK DLDLMLLDDS LLPLASDPLF STMSPEASKA SSRRSSFSME EGDVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the murine genes for bHLH-ZIP transcription factors TFEC and TFEB reveal a common gene organization for all MiT subfamily members." Rehli M., Den Elzen N., Cassady A.I., Ostrowski M.C., Hume D.A. Genomics 56:111-120(1999) [PubMed: 10036191] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-466, MASS SPECTROMETRY. Tissue: Liver.
[3]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-121, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF079095 mRNA. Translation: AAD20979.1. Different initiation.
IPI	IPI00314502.
UniGene	Mm.2305
3D structure databases
HSSP	HSSP built from PDB template 1AM9 based on UniProtKB P36956.
ModBase	Search...
PTM databases
PhosphoSite	Q9R210.
Genome annotation databases
Ensembl	ENSMUSG00000023990. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:103270. Tcfeb.
Phylogenomic databases
HOVERGEN	Q9R210.
Gene expression databases
ArrayExpress	Q9R210.
Bgee	Q9R210.
CleanEx	MM_TCFEB.
GermOnline	ENSMUSG00000023990. Mus musculus.
Family and domain databases
InterPro	IPR001092. HLH_basic. IPR011598. HLH_DNA_bd. [Graphical view]
Gene3D	G3DSA:4.10.280.10. HLH_DNA_bd. 1 hit.
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
PROSITE	PS50888. HLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

TFEB_MOUSE

Accession

Primary (citable) accession number: Q9R210

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	August 29, 2001
Last modified:	June 16, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)