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Protein

Vinexin

Gene

Sorbs3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes up-regulation of actin stress fiber formation.

GO - Molecular functioni

  • transcription factor binding Source: MGI
  • vinculin binding Source: MGI

GO - Biological processi

  • actin filament organization Source: InterPro
  • cell-substrate adhesion Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of MAPK cascade Source: MGI
  • positive regulation of stress fiber assembly Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-445355. Smooth Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Vinexin
Alternative name(s):
SH3 domain-containing protein SH3P3
SH3-containing adapter molecule 1
Short name:
SCAM-1
Sorbin and SH3 domain-containing protein 3
Gene namesi
Name:Sorbs3
Synonyms:Scam1, Sh3d4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:700013. Sorbs3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytoskeleton Source: UniProtKB-SubCell
  • focal adhesion Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 733733VinexinPRO_0000065831Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei412 – 4121PhosphoserineCombined sources
Modified residuei459 – 4591PhosphoserineCombined sources
Modified residuei594 – 5941Phosphoserine; by MAPK11 Publication
Modified residuei607 – 6071PhosphoserineBy similarity
Modified residuei610 – 6101PhosphoserineBy similarity
Modified residuei624 – 6241PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated at Ser-594 by MAPK1/ERK2 during cell spreading.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9R1Z8.
PaxDbiQ9R1Z8.
PRIDEiQ9R1Z8.

PTM databases

iPTMnetiQ9R1Z8.
PhosphoSiteiQ9R1Z8.

Expressioni

Gene expression databases

BgeeiQ9R1Z8.
CleanExiMM_SORBS3.
GenevisibleiQ9R1Z8. MM.

Interactioni

Subunit structurei

Interacts with vinculin by the first two SH3 domains and the proline rich region of vinculin. Binds to SOS (guanine nucleotide exchange factor of RAS and RAC), through its third SH3 domain. The formation of this complex is down-regulated by phosphorylation of SOS. Interacts with SAFB2, INPPL1/SHIP2 and SRCIN1 (By similarity). Interacts with DLG5 through its third SH3 domain. Interacts with SOCS7 and MAPK1/ERK2. Interacts with FASLG (By similarity).By similarity

GO - Molecular functioni

  • transcription factor binding Source: MGI
  • vinculin binding Source: MGI

Protein-protein interaction databases

BioGridi203212. 3 interactions.
IntActiQ9R1Z8. 5 interactions.
MINTiMINT-4998453.
STRINGi10090.ENSMUSP00000022682.

Structurei

3D structure databases

ProteinModelPortaliQ9R1Z8.
SMRiQ9R1Z8. Positions 418-589, 677-733.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini164 – 23269SoHoPROSITE-ProRule annotationAdd
BLAST
Domaini444 – 50360SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini518 – 57962SH3 2PROSITE-ProRule annotationAdd
BLAST
Domaini674 – 73360SH3 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni444 – 579136Binds to vinculinAdd
BLAST
Regioni674 – 73360Binds to SOSBy similarityAdd
BLAST

Sequence similaritiesi

Contains 3 SH3 domains.PROSITE-ProRule annotation
Contains 1 SoHo domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiENOG410KCZS. Eukaryota.
ENOG410XP8Q. LUCA.
GeneTreeiENSGT00760000119190.
HOGENOMiHOG000294090.
HOVERGENiHBG054842.
InParanoidiQ9R1Z8.
OMAiQFHDPAP.
OrthoDBiEOG7WX08W.
PhylomeDBiQ9R1Z8.
TreeFamiTF320680.

Family and domain databases

InterProiIPR001452. SH3_domain.
IPR003127. SoHo_dom.
IPR028510. Vinexin.
[Graphical view]
PANTHERiPTHR10663:SF204. PTHR10663:SF204. 1 hit.
PfamiPF00018. SH3_1. 1 hit.
PF14604. SH3_9. 2 hits.
PF02208. Sorb. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 3 hits.
SM00459. Sorb. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
PROSITEiPS50002. SH3. 3 hits.
PS50831. SOHO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R1Z8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARILGVGRS SASSLNNKED NESDVALLSP KDPNRVHTKE QLAHPASSNL
60 70 80 90 100
DPSMQGLPAG LSLDDFIPGH LRTHIGSSSR GTRVPVIRNG GSNTLNFQFH
110 120 130 140 150
DPAPRTVCNG CPPPRRDGSL NPDPAWYQTW PGPGSRPSMS PKPPASQHAQ
160 170 180 190 200
NWSATWTKDS KRQDKRWVKY EGIGPVDESG MPIAPRSSVD SPRDWYRRMF
210 220 230 240 250
QQIHRKMPDL QLDWTLEDPP KVVSARASSA EPRHLGTLQR PASRPGTTET
260 270 280 290 300
SSGRNWNHSE ETSRNTFNYN FRPSSSGLHP PNQVPRHREK VENVWTEDSW
310 320 330 340 350
NQFLHELETG HKPKKPLVDD PVEKPAQPIE VLLERELAKL SAELDKDLRA
360 370 380 390 400
IETRLPSPKN SQAPRRPLEQ PGLEQQPSAR LSSAWRPNSP HAPYFSSSRP
410 420 430 440 450
LSPHRMADGG GSPFLGRRDF VYPSSAREPS ASERGSSPSR KEEKKRKAAR
460 470 480 490 500
LKFDFQAQSP KELSLQKGDI VYIHKEVDKN WLEGEHHGRL GIFPANYVEV
510 520 530 540 550
LPADEIPKPI KPPTYQVLEY GDAVAQYTFK GDLEVELSFR KGERICLIRK
560 570 580 590 600
VNEHWYEGRI TGTGRQGIFP ASYVQINREP RLRLCDDGPQ LPASPNPTTT
610 620 630 640 650
AHLSSHSHPS SIPVDPTDWG GRTSPRRSAF PFPITLQEPR SQTQSLNTPG
660 670 680 690 700
PTLSHPRATS RPINLGPSSP NTEIHWTPYR AMYQYRPQNE DELELREGDR
710 720 730
VDVMQQCDDG WFVGVSRRTQ KFGTFPGNYV APV
Length:733
Mass (Da):82,349
Last modified:May 1, 2000 - v1
Checksum:iD7716FA1D4F0E3CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064806 mRNA. Translation: AAD32303.1.
U58889 mRNA. Translation: AAC52642.1.
CCDSiCCDS27249.1.
RefSeqiNP_035496.1. NM_011366.3.
UniGeneiMm.5068.

Genome annotation databases

EnsembliENSMUST00000022682; ENSMUSP00000022682; ENSMUSG00000022091.
GeneIDi20410.
KEGGimmu:20410.
UCSCiuc007unk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064806 mRNA. Translation: AAD32303.1.
U58889 mRNA. Translation: AAC52642.1.
CCDSiCCDS27249.1.
RefSeqiNP_035496.1. NM_011366.3.
UniGeneiMm.5068.

3D structure databases

ProteinModelPortaliQ9R1Z8.
SMRiQ9R1Z8. Positions 418-589, 677-733.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203212. 3 interactions.
IntActiQ9R1Z8. 5 interactions.
MINTiMINT-4998453.
STRINGi10090.ENSMUSP00000022682.

PTM databases

iPTMnetiQ9R1Z8.
PhosphoSiteiQ9R1Z8.

Proteomic databases

MaxQBiQ9R1Z8.
PaxDbiQ9R1Z8.
PRIDEiQ9R1Z8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022682; ENSMUSP00000022682; ENSMUSG00000022091.
GeneIDi20410.
KEGGimmu:20410.
UCSCiuc007unk.1. mouse.

Organism-specific databases

CTDi10174.
MGIiMGI:700013. Sorbs3.

Phylogenomic databases

eggNOGiENOG410KCZS. Eukaryota.
ENOG410XP8Q. LUCA.
GeneTreeiENSGT00760000119190.
HOGENOMiHOG000294090.
HOVERGENiHBG054842.
InParanoidiQ9R1Z8.
OMAiQFHDPAP.
OrthoDBiEOG7WX08W.
PhylomeDBiQ9R1Z8.
TreeFamiTF320680.

Enzyme and pathway databases

ReactomeiR-MMU-445355. Smooth Muscle Contraction.

Miscellaneous databases

ChiTaRSiSorbs3. mouse.
NextBioi298392.
PROiQ9R1Z8.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R1Z8.
CleanExiMM_SORBS3.
GenevisibleiQ9R1Z8. MM.

Family and domain databases

InterProiIPR001452. SH3_domain.
IPR003127. SoHo_dom.
IPR028510. Vinexin.
[Graphical view]
PANTHERiPTHR10663:SF204. PTHR10663:SF204. 1 hit.
PfamiPF00018. SH3_1. 1 hit.
PF14604. SH3_9. 2 hits.
PF02208. Sorb. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 3 hits.
SM00459. Sorb. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
PROSITEiPS50002. SH3. 3 hits.
PS50831. SOHO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Vinexin: a novel vinculin-binding protein with multiple SH3 domains enhances actin cytoskeletal organization."
    Kioka N., Sakata S., Kawauchi T., Amachi T., Akiyama S.K., Okazaki K., Yaen C., Yamada K.M., Aota S.
    J. Cell Biol. 144:59-69(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.
  2. "Cloning of ligand targets: systematic isolation of SH3 domain-containing proteins."
    Sparks A.B., Hoffman N.G., McConnell S.J., Fowlkes D.M., Kay B.K.
    Nat. Biotechnol. 14:741-744(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 559-733.
    Tissue: Embryo.
  3. "Interaction of lp-dlg/KIAA0583, a membrane-associated guanylate kinase family protein, with vinexin and beta-catenin at sites of cell-cell contact."
    Wakabayashi M., Ito T., Mitsushima M., Aizawa S., Ueda K., Amachi T., Kioka N.
    J. Biol. Chem. 278:21709-21714(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH DLG5.
  4. "Extracellular signal-regulated kinase activated by epidermal growth factor and cell adhesion interacts with and phosphorylates vinexin."
    Mitsushima M., Suwa A., Amachi T., Ueda K., Kioka N.
    J. Biol. Chem. 279:34570-34577(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-594, INTERACTION WITH MAPK1/ERK2, SUBCELLULAR LOCATION.
  5. "The suppressor of cytokine signaling (SOCS)-7 interacts with the actin cytoskeleton through vinexin."
    Martens N., Wery M., Wang P., Braet F., Gertler A., Hooghe R., Vandenhaute J., Hooghe-Peters E.L.
    Exp. Cell Res. 298:239-248(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SOCS7.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-412, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-412 AND SER-459, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Kidney and Lung.

Entry informationi

Entry nameiVINEX_MOUSE
AccessioniPrimary (citable) accession number: Q9R1Z8
Secondary accession number(s): Q62423
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.