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Q9R1Z7

- PTPS_MOUSE

UniProt

Q9R1Z7 - PTPS_MOUSE

Protein

6-pyruvoyl tetrahydrobiopterin synthase

Gene

Pts

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin.

    Catalytic activityi

    7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi23 – 231ZincPROSITE-ProRule annotation
    Active sitei42 – 421Proton acceptorPROSITE-ProRule annotation
    Metal bindingi48 – 481ZincPROSITE-ProRule annotation
    Metal bindingi50 – 501ZincPROSITE-ProRule annotation
    Active sitei89 – 891Charge relay systemPROSITE-ProRule annotation
    Active sitei133 – 1331Charge relay systemPROSITE-ProRule annotation

    GO - Molecular functioni

    1. 6-pyruvoyltetrahydropterin synthase activity Source: MGI
    2. identical protein binding Source: MGI
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: MGI
    5. protein homodimerization activity Source: MGI

    GO - Biological processi

    1. tetrahydrobiopterin biosynthetic process Source: MGI

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Tetrahydrobiopterin biosynthesis

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_199047. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
    UniPathwayiUPA00849; UER00819.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    6-pyruvoyl tetrahydrobiopterin synthase (EC:4.2.3.12)
    Short name:
    PTP synthase
    Short name:
    PTPS
    Gene namesi
    Name:Pts
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1338783. Pts.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 1441446-pyruvoyl tetrahydrobiopterin synthasePRO_0000057915Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei18 – 181PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation of Ser-18 is required for maximal enzyme activity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9R1Z7.
    PaxDbiQ9R1Z7.
    PRIDEiQ9R1Z7.

    PTM databases

    PhosphoSiteiQ9R1Z7.

    Expressioni

    Gene expression databases

    BgeeiQ9R1Z7.
    CleanExiMM_PTS.
    GenevestigatoriQ9R1Z7.

    Interactioni

    Subunit structurei

    Homohexamer formed of two homotrimers in a head to head fashion.By similarity

    Protein-protein interaction databases

    IntActiQ9R1Z7. 5 interactions.
    MINTiMINT-217930.
    STRINGi10090.ENSMUSP00000034570.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R1Z7.
    SMRiQ9R1Z7. Positions 17-144.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PTPS family.Curated

    Phylogenomic databases

    eggNOGiCOG0720.
    GeneTreeiENSGT00390000002752.
    HOGENOMiHOG000225069.
    HOVERGENiHBG004358.
    InParanoidiQ9R1Z7.
    KOiK01737.
    OMAiCHRLHSK.
    OrthoDBiEOG7NSB3V.
    PhylomeDBiQ9R1Z7.
    TreeFamiTF105796.

    Family and domain databases

    InterProiIPR007115. 6-PTP_synth/QueD.
    IPR022470. PTPS_Cys_AS.
    IPR022469. PTPS_His_AS.
    [Graphical view]
    PANTHERiPTHR12589. PTHR12589. 1 hit.
    PfamiPF01242. PTPS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006113. PTP_synth. 1 hit.
    TIGRFAMsiTIGR00039. 6PTHBS. 1 hit.
    PROSITEiPS00987. PTPS_1. 1 hit.
    PS00988. PTPS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9R1Z7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAAGDLRRR ARLSRLVSFS ASHRLHSPSL SDEENLRVFG KCNNPNGHGH    50
    NYKVVVTVHG EIDPVTGMVM NLTDLKEYME EAIMKPLDHK NLDLDVPYFA 100
    DAVSTTENVA VYIWESLQKL LPVGALYKVK VFETDNNIVV YKGE 144
    Length:144
    Mass (Da):16,188
    Last modified:November 1, 2002 - v2
    Checksum:iAAB034E27ED7A9E3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 61D → G in AAD15827. (PubMed:9894812)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF061880, AF061878, AF061879 Genomic DNA. Translation: AAD15827.1.
    AF043225 mRNA. Translation: AAD02249.1.
    AK002614 mRNA. Translation: BAB22231.1.
    AK146987 mRNA. Translation: BAE27590.1.
    BC029013 mRNA. Translation: AAH29013.1.
    CCDSiCCDS23164.1.
    RefSeqiNP_035350.1. NM_011220.2.
    UniGeneiMm.35856.

    Genome annotation databases

    EnsembliENSMUST00000034570; ENSMUSP00000034570; ENSMUSG00000032067.
    GeneIDi19286.
    KEGGimmu:19286.
    UCSCiuc009pjp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF061880 , AF061878 , AF061879 Genomic DNA. Translation: AAD15827.1 .
    AF043225 mRNA. Translation: AAD02249.1 .
    AK002614 mRNA. Translation: BAB22231.1 .
    AK146987 mRNA. Translation: BAE27590.1 .
    BC029013 mRNA. Translation: AAH29013.1 .
    CCDSi CCDS23164.1.
    RefSeqi NP_035350.1. NM_011220.2.
    UniGenei Mm.35856.

    3D structure databases

    ProteinModelPortali Q9R1Z7.
    SMRi Q9R1Z7. Positions 17-144.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9R1Z7. 5 interactions.
    MINTi MINT-217930.
    STRINGi 10090.ENSMUSP00000034570.

    PTM databases

    PhosphoSitei Q9R1Z7.

    Proteomic databases

    MaxQBi Q9R1Z7.
    PaxDbi Q9R1Z7.
    PRIDEi Q9R1Z7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034570 ; ENSMUSP00000034570 ; ENSMUSG00000032067 .
    GeneIDi 19286.
    KEGGi mmu:19286.
    UCSCi uc009pjp.1. mouse.

    Organism-specific databases

    CTDi 5805.
    MGIi MGI:1338783. Pts.

    Phylogenomic databases

    eggNOGi COG0720.
    GeneTreei ENSGT00390000002752.
    HOGENOMi HOG000225069.
    HOVERGENi HBG004358.
    InParanoidi Q9R1Z7.
    KOi K01737.
    OMAi CHRLHSK.
    OrthoDBi EOG7NSB3V.
    PhylomeDBi Q9R1Z7.
    TreeFami TF105796.

    Enzyme and pathway databases

    UniPathwayi UPA00849 ; UER00819 .
    Reactomei REACT_199047. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

    Miscellaneous databases

    ChiTaRSi PTS. mouse.
    NextBioi 296210.
    PROi Q9R1Z7.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9R1Z7.
    CleanExi MM_PTS.
    Genevestigatori Q9R1Z7.

    Family and domain databases

    InterProi IPR007115. 6-PTP_synth/QueD.
    IPR022470. PTPS_Cys_AS.
    IPR022469. PTPS_His_AS.
    [Graphical view ]
    PANTHERi PTHR12589. PTHR12589. 1 hit.
    Pfami PF01242. PTPS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006113. PTP_synth. 1 hit.
    TIGRFAMsi TIGR00039. 6PTHBS. 1 hit.
    PROSITEi PS00987. PTPS_1. 1 hit.
    PS00988. PTPS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure, genomic localization and recombinant expression of the mouse 6-pyruvoyl-tetrahydropterin synthase gene."
      Turri M.O., Ilg E.C., Thony B., Blau N.
      Biol. Chem. 379:1441-1447(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Ola.
    2. Thony B., Turri M., Blau N.
      Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: C57BL/6J.
      Tissue: Placenta.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Kidney and Stomach.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Retina.

    Entry informationi

    Entry nameiPTPS_MOUSE
    AccessioniPrimary (citable) accession number: Q9R1Z7
    Secondary accession number(s): Q3UIB6, Q9Z2N2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 2002
    Last sequence update: November 1, 2002
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3