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Protein

6-pyruvoyl tetrahydrobiopterin synthase

Gene

Pts

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin.

Miscellaneous

The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit.

Catalytic activityi

7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: tetrahydrobiopterin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. 6-pyruvoyl tetrahydrobiopterin synthase (Pts)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway tetrahydrobiopterin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrobiopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi23ZincPROSITE-ProRule annotation1
Active sitei42Proton acceptorPROSITE-ProRule annotation1
Metal bindingi48ZincPROSITE-ProRule annotation1
Metal bindingi50ZincPROSITE-ProRule annotation1
Active sitei89Charge relay systemPROSITE-ProRule annotation1
Active sitei133Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

  • 6-pyruvoyltetrahydropterin synthase activity Source: MGI
  • identical protein binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • protein homodimerization activity Source: MGI

GO - Biological processi

  • tetrahydrobiopterin biosynthetic process Source: MGI

Keywordsi

Molecular functionLyase
Biological processTetrahydrobiopterin biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
UniPathwayiUPA00849; UER00819

Names & Taxonomyi

Protein namesi
Recommended name:
6-pyruvoyl tetrahydrobiopterin synthase (EC:4.2.3.12)
Short name:
PTP synthase
Short name:
PTPS
Gene namesi
Name:Pts
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1338783 Pts

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000579151 – 1446-pyruvoyl tetrahydrobiopterin synthaseAdd BLAST144

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineBy similarity1
Modified residuei27PhosphoserineCombined sources1
Modified residuei127PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylation of Ser-18 is required for maximal enzyme activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9R1Z7
MaxQBiQ9R1Z7
PaxDbiQ9R1Z7
PeptideAtlasiQ9R1Z7
PRIDEiQ9R1Z7

PTM databases

iPTMnetiQ9R1Z7
PhosphoSitePlusiQ9R1Z7

Expressioni

Gene expression databases

BgeeiENSMUSG00000032067
CleanExiMM_PTS
ExpressionAtlasiQ9R1Z7 baseline and differential
GenevisibleiQ9R1Z7 MM

Interactioni

Subunit structurei

Homohexamer formed of two homotrimers in a head to head fashion.By similarity

GO - Molecular functioni

  • identical protein binding Source: MGI
  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

IntActiQ9R1Z7, 5 interactors
MINTiQ9R1Z7
STRINGi10090.ENSMUSP00000034570

Structurei

3D structure databases

ProteinModelPortaliQ9R1Z7
SMRiQ9R1Z7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PTPS family.Curated

Phylogenomic databases

eggNOGiKOG4105 Eukaryota
COG0720 LUCA
GeneTreeiENSGT00390000002752
HOGENOMiHOG000225069
HOVERGENiHBG004358
InParanoidiQ9R1Z7
KOiK01737
OMAiNIAVVIW
OrthoDBiEOG091G0TKH
PhylomeDBiQ9R1Z7
TreeFamiTF105796

Family and domain databases

Gene3Di3.30.479.10, 1 hit
InterProiView protein in InterPro
IPR007115 6-PTP_synth/QueD
IPR038418 6-PTP_synth/QueD_sf
IPR022470 PTPS_Cys_AS
IPR022469 PTPS_His_AS
PANTHERiPTHR12589 PTHR12589, 1 hit
PfamiView protein in Pfam
PF01242 PTPS, 1 hit
PIRSFiPIRSF006113 PTP_synth, 1 hit
TIGRFAMsiTIGR00039 6PTHBS, 1 hit
PROSITEiView protein in PROSITE
PS00987 PTPS_1, 1 hit
PS00988 PTPS_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9R1Z7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAAGDLRRR ARLSRLVSFS ASHRLHSPSL SDEENLRVFG KCNNPNGHGH
60 70 80 90 100
NYKVVVTVHG EIDPVTGMVM NLTDLKEYME EAIMKPLDHK NLDLDVPYFA
110 120 130 140
DAVSTTENVA VYIWESLQKL LPVGALYKVK VFETDNNIVV YKGE
Length:144
Mass (Da):16,188
Last modified:November 1, 2002 - v2
Checksum:iAAB034E27ED7A9E3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6D → G in AAD15827 (PubMed:9894812).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061880, AF061878, AF061879 Genomic DNA Translation: AAD15827.1
AF043225 mRNA Translation: AAD02249.1
AK002614 mRNA Translation: BAB22231.1
AK146987 mRNA Translation: BAE27590.1
BC029013 mRNA Translation: AAH29013.1
CCDSiCCDS23164.1
RefSeqiNP_035350.1, NM_011220.2
UniGeneiMm.35856

Genome annotation databases

EnsembliENSMUST00000034570; ENSMUSP00000034570; ENSMUSG00000032067
GeneIDi19286
KEGGimmu:19286
UCSCiuc009pjp.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPTPS_MOUSE
AccessioniPrimary (citable) accession number: Q9R1Z7
Secondary accession number(s): Q3UIB6, Q9Z2N2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: November 1, 2002
Last modified: May 23, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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