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Protein

6-pyruvoyl tetrahydrobiopterin synthase

Gene

Pts

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin.

Catalytic activityi

7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: tetrahydrobiopterin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. 6-pyruvoyl tetrahydrobiopterin synthase (Pts)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway tetrahydrobiopterin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrobiopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi23ZincPROSITE-ProRule annotation1
Active sitei42Proton acceptorPROSITE-ProRule annotation1
Metal bindingi48ZincPROSITE-ProRule annotation1
Metal bindingi50ZincPROSITE-ProRule annotation1
Active sitei89Charge relay systemPROSITE-ProRule annotation1
Active sitei133Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

  • 6-pyruvoyltetrahydropterin synthase activity Source: MGI
  • identical protein binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • protein homodimerization activity Source: MGI

GO - Biological processi

  • tetrahydrobiopterin biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tetrahydrobiopterin biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
UniPathwayiUPA00849; UER00819.

Names & Taxonomyi

Protein namesi
Recommended name:
6-pyruvoyl tetrahydrobiopterin synthase (EC:4.2.3.12)
Short name:
PTP synthase
Short name:
PTPS
Gene namesi
Name:Pts
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1338783. Pts.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000579151 – 1446-pyruvoyl tetrahydrobiopterin synthaseAdd BLAST144

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineBy similarity1
Modified residuei27PhosphoserineCombined sources1
Modified residuei127PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylation of Ser-18 is required for maximal enzyme activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9R1Z7.
MaxQBiQ9R1Z7.
PaxDbiQ9R1Z7.
PeptideAtlasiQ9R1Z7.
PRIDEiQ9R1Z7.

PTM databases

iPTMnetiQ9R1Z7.
PhosphoSitePlusiQ9R1Z7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032067.
CleanExiMM_PTS.
GenevisibleiQ9R1Z7. MM.

Interactioni

Subunit structurei

Homohexamer formed of two homotrimers in a head to head fashion.By similarity

GO - Molecular functioni

  • identical protein binding Source: MGI
  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

IntActiQ9R1Z7. 5 interactors.
MINTiMINT-217930.
STRINGi10090.ENSMUSP00000034570.

Structurei

3D structure databases

ProteinModelPortaliQ9R1Z7.
SMRiQ9R1Z7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PTPS family.Curated

Phylogenomic databases

eggNOGiKOG4105. Eukaryota.
COG0720. LUCA.
GeneTreeiENSGT00390000002752.
HOGENOMiHOG000225069.
HOVERGENiHBG004358.
InParanoidiQ9R1Z7.
KOiK01737.
OMAiHGHNYVW.
OrthoDBiEOG091G0TKH.
PhylomeDBiQ9R1Z7.
TreeFamiTF105796.

Family and domain databases

InterProiIPR007115. 6-PTP_synth/QueD.
IPR022470. PTPS_Cys_AS.
IPR022469. PTPS_His_AS.
[Graphical view]
PANTHERiPTHR12589. PTHR12589. 1 hit.
PfamiPF01242. PTPS. 1 hit.
[Graphical view]
PIRSFiPIRSF006113. PTP_synth. 1 hit.
TIGRFAMsiTIGR00039. 6PTHBS. 1 hit.
PROSITEiPS00987. PTPS_1. 1 hit.
PS00988. PTPS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R1Z7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAAGDLRRR ARLSRLVSFS ASHRLHSPSL SDEENLRVFG KCNNPNGHGH
60 70 80 90 100
NYKVVVTVHG EIDPVTGMVM NLTDLKEYME EAIMKPLDHK NLDLDVPYFA
110 120 130 140
DAVSTTENVA VYIWESLQKL LPVGALYKVK VFETDNNIVV YKGE
Length:144
Mass (Da):16,188
Last modified:November 1, 2002 - v2
Checksum:iAAB034E27ED7A9E3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6D → G in AAD15827 (PubMed:9894812).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061880, AF061878, AF061879 Genomic DNA. Translation: AAD15827.1.
AF043225 mRNA. Translation: AAD02249.1.
AK002614 mRNA. Translation: BAB22231.1.
AK146987 mRNA. Translation: BAE27590.1.
BC029013 mRNA. Translation: AAH29013.1.
CCDSiCCDS23164.1.
RefSeqiNP_035350.1. NM_011220.2.
UniGeneiMm.35856.

Genome annotation databases

EnsembliENSMUST00000034570; ENSMUSP00000034570; ENSMUSG00000032067.
GeneIDi19286.
KEGGimmu:19286.
UCSCiuc009pjp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061880, AF061878, AF061879 Genomic DNA. Translation: AAD15827.1.
AF043225 mRNA. Translation: AAD02249.1.
AK002614 mRNA. Translation: BAB22231.1.
AK146987 mRNA. Translation: BAE27590.1.
BC029013 mRNA. Translation: AAH29013.1.
CCDSiCCDS23164.1.
RefSeqiNP_035350.1. NM_011220.2.
UniGeneiMm.35856.

3D structure databases

ProteinModelPortaliQ9R1Z7.
SMRiQ9R1Z7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9R1Z7. 5 interactors.
MINTiMINT-217930.
STRINGi10090.ENSMUSP00000034570.

PTM databases

iPTMnetiQ9R1Z7.
PhosphoSitePlusiQ9R1Z7.

Proteomic databases

EPDiQ9R1Z7.
MaxQBiQ9R1Z7.
PaxDbiQ9R1Z7.
PeptideAtlasiQ9R1Z7.
PRIDEiQ9R1Z7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034570; ENSMUSP00000034570; ENSMUSG00000032067.
GeneIDi19286.
KEGGimmu:19286.
UCSCiuc009pjp.1. mouse.

Organism-specific databases

CTDi5805.
MGIiMGI:1338783. Pts.

Phylogenomic databases

eggNOGiKOG4105. Eukaryota.
COG0720. LUCA.
GeneTreeiENSGT00390000002752.
HOGENOMiHOG000225069.
HOVERGENiHBG004358.
InParanoidiQ9R1Z7.
KOiK01737.
OMAiHGHNYVW.
OrthoDBiEOG091G0TKH.
PhylomeDBiQ9R1Z7.
TreeFamiTF105796.

Enzyme and pathway databases

UniPathwayiUPA00849; UER00819.
ReactomeiR-MMU-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

Miscellaneous databases

ChiTaRSiPts. mouse.
PROiQ9R1Z7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032067.
CleanExiMM_PTS.
GenevisibleiQ9R1Z7. MM.

Family and domain databases

InterProiIPR007115. 6-PTP_synth/QueD.
IPR022470. PTPS_Cys_AS.
IPR022469. PTPS_His_AS.
[Graphical view]
PANTHERiPTHR12589. PTHR12589. 1 hit.
PfamiPF01242. PTPS. 1 hit.
[Graphical view]
PIRSFiPIRSF006113. PTP_synth. 1 hit.
TIGRFAMsiTIGR00039. 6PTHBS. 1 hit.
PROSITEiPS00987. PTPS_1. 1 hit.
PS00988. PTPS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTPS_MOUSE
AccessioniPrimary (citable) accession number: Q9R1Z7
Secondary accession number(s): Q3UIB6, Q9Z2N2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: November 1, 2002
Last modified: November 2, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.